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The map label for this gene is entA [C]

Identifier: 114799268

GI number: 114799268

Start: 438602

End: 439570

Strand: Reverse

Name: entA [C]

Synonym: HNE_0442

Alternate gene names: 114799268

Gene position: 439570-438602 (Counterclockwise)

Preceding gene: 114798291

Following gene: 114798677

Centisome position: 11.86

GC content: 63.36

Gene sequence:

>969_bases
ATGGCGGCTAATGATCAGGTACCTGTCGGCTCAGGCTTTCACGCAAAGAGCACCGCGAGGGAGGTTGTTGAGGGGATCAG
CCTCTCGGGCAAGCACGCGATTGTGACCGGCGGATATTCGGGCATCGGCCTTGAGACAGTCCGCGCGCTGGCGGGCGCCG
GCGCGAAGGTGACGGTGCCAGCCCGCCGGCCGGACGCGGCAAAAGACGCGCTCACCGGGATCAATGGCGTTACGGTTGCC
GCGATGGACCTGGCCGATCTCGGCAGTGTGCGCCGCTTTGCGCAGGATTATTCTGCGGCGGCGCCGAAGCTGGACCTGCT
CATAAACAATGCCGGGATCATGGCGTGTCCGCTCGCCCACGTTGGCGATGGCTGGGAAGCCCAGTTCGGCACCAACCATC
TGGGTCACCTTGCCCTGTATCAGGGTCTCCTGCCCGCGTTGCGCGCCGCAAATGGCGCCCGCGTGGTAGCGCTCTCTTCC
ACCGGACATGTCCGCTCCGATGTGATCTGGGACGATCCCAACTATACGTCCCGCGCCTATGACAAATGGGAAGCCTACGG
ACAGTCCAAATCCGCCAATGCGCTGTTCGCGCTGGGGGTGGACATGCGGGAAAAAGCCAATGGCATTCGCGCCTTCTCCG
TGCATCCCGGCGGCATCTTTACGAACCTTCAGCGCCATCTGCCCGACGAGGAAATGGTGGCGCTCGGCTGGAAAAGTCCT
GACGGCACGATCCCGGAGATGATCCAGGCGATGTTCAAGACGCCGGAACAGGGCGCCTCCACCACCGTGTGGGCCGCAAC
GGCGCCAAAGCTGAACGGGAAAGGCGGCGTTTATTGTGAAGACTGTGACATCGCCCGCGCCGCGACGCCCGACAGCAAAC
GCTGGGAACATGCCCGCGCCTGGATCAGCGATGATGCCCGCGCCGAGAAACTGTGGGCGATGAGCGACACATTCCTGGCG
AATGCATGA

Upstream 100 bases:

>100_bases
GGCAAGGCTGGGGCAAAGCCGGTATTAGATTGCAGTATCCCCCAGATAGAGAGGGGGCTAGCATTGGGACAATACGTAGA
AACACATGGGAGAAATGACG

Downstream 100 bases:

>100_bases
GAGTCTGACGGGTTCAGATTGACGCACCCCGGCTTTCAGCGCACGAGAGCGCCATGAGCGAAACGCAACTCCCGACCACC
GCCCGGCAGGCTCCGCGGCG

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MAANDQVPVGSGFHAKSTAREVVEGISLSGKHAIVTGGYSGIGLETVRALAGAGAKVTVPARRPDAAKDALTGINGVTVA
AMDLADLGSVRRFAQDYSAAAPKLDLLINNAGIMACPLAHVGDGWEAQFGTNHLGHLALYQGLLPALRAANGARVVALSS
TGHVRSDVIWDDPNYTSRAYDKWEAYGQSKSANALFALGVDMREKANGIRAFSVHPGGIFTNLQRHLPDEEMVALGWKSP
DGTIPEMIQAMFKTPEQGASTTVWAATAPKLNGKGGVYCEDCDIARAATPDSKRWEHARAWISDDARAEKLWAMSDTFLA
NA

Sequences:

>Translated_322_residues
MAANDQVPVGSGFHAKSTAREVVEGISLSGKHAIVTGGYSGIGLETVRALAGAGAKVTVPARRPDAAKDALTGINGVTVA
AMDLADLGSVRRFAQDYSAAAPKLDLLINNAGIMACPLAHVGDGWEAQFGTNHLGHLALYQGLLPALRAANGARVVALSS
TGHVRSDVIWDDPNYTSRAYDKWEAYGQSKSANALFALGVDMREKANGIRAFSVHPGGIFTNLQRHLPDEEMVALGWKSP
DGTIPEMIQAMFKTPEQGASTTVWAATAPKLNGKGGVYCEDCDIARAATPDSKRWEHARAWISDDARAEKLWAMSDTFLA
NA
>Mature_321_residues
AANDQVPVGSGFHAKSTAREVVEGISLSGKHAIVTGGYSGIGLETVRALAGAGAKVTVPARRPDAAKDALTGINGVTVAA
MDLADLGSVRRFAQDYSAAAPKLDLLINNAGIMACPLAHVGDGWEAQFGTNHLGHLALYQGLLPALRAANGARVVALSST
GHVRSDVIWDDPNYTSRAYDKWEAYGQSKSANALFALGVDMREKANGIRAFSVHPGGIFTNLQRHLPDEEMVALGWKSPD
GTIPEMIQAMFKTPEQGASTTVWAATAPKLNGKGGVYCEDCDIARAATPDSKRWEHARAWISDDARAEKLWAMSDTFLAN
A

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI10190746, Length=319, Percent_Identity=32.2884012539185, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI186928839, Length=304, Percent_Identity=34.2105263157895, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI166795268, Length=306, Percent_Identity=33.9869281045752, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI225579078, Length=295, Percent_Identity=34.2372881355932, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI7706523, Length=321, Percent_Identity=31.4641744548287, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI193804850, Length=261, Percent_Identity=35.632183908046, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI19923931, Length=231, Percent_Identity=35.0649350649351, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI146231950, Length=302, Percent_Identity=32.4503311258278, Blast_Score=114, Evalue=8e-26,
Organism=Escherichia coli, GI1786812, Length=210, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193207620, Length=317, Percent_Identity=32.1766561514196, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17532805, Length=317, Percent_Identity=32.1766561514196, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71992402, Length=286, Percent_Identity=34.2657342657343, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17532791, Length=333, Percent_Identity=30.6306306306306, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17558006, Length=279, Percent_Identity=31.5412186379928, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17535059, Length=289, Percent_Identity=30.7958477508651, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32565532, Length=232, Percent_Identity=32.3275862068966, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17505416, Length=274, Percent_Identity=27.007299270073, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI72001364, Length=309, Percent_Identity=28.1553398058252, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324820, Length=282, Percent_Identity=29.0780141843972, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322227, Length=240, Percent_Identity=24.1666666666667, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI22024069, Length=296, Percent_Identity=34.7972972972973, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45554248, Length=296, Percent_Identity=35.8108108108108, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24640117, Length=296, Percent_Identity=35.8108108108108, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI28573296, Length=294, Percent_Identity=36.3945578231293, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19921754, Length=299, Percent_Identity=33.7792642140468, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24586328, Length=294, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161076371, Length=294, Percent_Identity=35.0340136054422, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI20129347, Length=322, Percent_Identity=28.8819875776398, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24656154, Length=286, Percent_Identity=34.2657342657343, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19922628, Length=286, Percent_Identity=34.2657342657343, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647946, Length=305, Percent_Identity=31.8032786885246, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45553409, Length=290, Percent_Identity=32.4137931034483, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24647948, Length=290, Percent_Identity=32.4137931034483, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24647931, Length=280, Percent_Identity=29.6428571428571, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI23397609, Length=220, Percent_Identity=31.3636363636364, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 34068; Mature: 33936

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANDQVPVGSGFHAKSTAREVVEGISLSGKHAIVTGGYSGIGLETVRALAGAGAKVTVP
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEC
ARRPDAAKDALTGINGVTVAAMDLADLGSVRRFAQDYSAAAPKLDLLINNAGIMACPLAH
CCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECHHH
VGDGWEAQFGTNHLGHLALYQGLLPALRAANGARVVALSSTGHVRSDVIWDDPNYTSRAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCHHHH
DKWEAYGQSKSANALFALGVDMREKANGIRAFSVHPGGIFTNLQRHLPDEEMVALGWKSP
HHHHHHCCCCCCCEEEEECCCHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCC
DGTIPEMIQAMFKTPEQGASTTVWAATAPKLNGKGGVYCEDCDIARAATPDSKRWEHARA
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
WISDDARAEKLWAMSDTFLANA
HHCCCHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
AANDQVPVGSGFHAKSTAREVVEGISLSGKHAIVTGGYSGIGLETVRALAGAGAKVTVP
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEC
ARRPDAAKDALTGINGVTVAAMDLADLGSVRRFAQDYSAAAPKLDLLINNAGIMACPLAH
CCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECHHH
VGDGWEAQFGTNHLGHLALYQGLLPALRAANGARVVALSSTGHVRSDVIWDDPNYTSRAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCHHHH
DKWEAYGQSKSANALFALGVDMREKANGIRAFSVHPGGIFTNLQRHLPDEEMVALGWKSP
HHHHHHCCCCCCCEEEEECCCHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCC
DGTIPEMIQAMFKTPEQGASTTVWAATAPKLNGKGGVYCEDCDIARAATPDSKRWEHARA
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
WISDDARAEKLWAMSDTFLANA
HHCCCHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]