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The map label for this gene is phaA [H]

Identifier: 114798910

GI number: 114798910

Start: 2100593

End: 2102281

Strand: Reverse

Name: phaA [H]

Synonym: HNE_2024

Alternate gene names: 114798910

Gene position: 2102281-2100593 (Counterclockwise)

Preceding gene: 114798462

Following gene: 114798279

Centisome position: 56.74

GC content: 59.15

Gene sequence:

>1689_bases
ATGACGTCACCGAAAGACGAGATTGCCCGCAATGCGGCTGAAAACACCGCCGCGCTGAACCCGCTGCTGGGCGGCTTCAA
CCGCCAGGAACTGCTCGGCGCCGTGGGTCTGATGCTGCGCTCCACGATGACCAACCCGGTCACCACCGCCAGGACCGCCG
GCAAGATCACGGCCGAAAACACCCAGATCCTGCTGGGCAAGTCCAAGCGCGAAGCCGACAAGAAAGACCGCCGCTTCAAG
GACCCCGCCTGGGAGCATAATCCCTTCTACAAGCGCGGCATGCAGACCTATCTGGCCACCCAGGAACACCTCCACGCCTG
GGTCAACGAGATCAAGATGGGCGAGCTGGAACAGGCGCGCGCCAAATTCGTCATGGGCATGATCACCGATGCCCTCGCGC
CCACAAACTCCCTCGTGGGCAATCCGGCCGCCACCAAGCGCGTCGTGGATTCGGGCGGCCTCTCCCTGCTCAAGGGCATG
AAAAACCTCTACGACGACCTCACCAAGAATGGCGGTCTCCCGTCCCAGGTCGATAAACGTCCCTTCAAGGTTGGTGAAAA
TCTCGCCGTTTCAAAAGGGCAGGTGGTCTGGAAAAACGAGATGCTGGAGCTGATCCAGTATGCCCCGCTCACCGAGAAGG
TCCACAAGACCCCGATCCTGATAATTCCCCCACAGATCAACAAATTCTACGCCATGGACCTCACGCCGATGACGTCGATG
GTCCAGTTCCTCCTCGCGATGGAACAGCAGACCTTTGTGATTTCGTGGCGCAACCCCACCAAGAAGCACAAAGACTGGGG
GATGAACGACTATATCGACAGCCTCGTCCAGGCCAGCGAAGTCATCCGCAAGATCACGAAGTCGCCTAAGATCAACGTCT
CCGGCGCCTGCTCGGGCGGCATCACCACGGCCACCTTCGCGAGCCTTCTTGCCGCCGCCGATGACAAACGCATCAACTCG
CTCACCTTCATGGTCTGCGTGCTCAACCCCCAGCGCGACGACAGCGACATTGGCCAGATCGTGTCGGATGGCAGCCTCGA
AATCGCGCGCAAGTATTCCAAATCCCGCGGCATCCTGAAGGGCGATGACCTTGCCCGCATGTTTGCCTGGATGCGCCCGA
ACGACCTCATTTGGAACTATGTCGTCAACAACTATCTCATGGGCGAAGATCCTCCGCCCTATGACGTTCTGTTCTGGAAC
AACGACACAACCAACCTCCCGGCCCAGCTGCATTCAGATTATCTGGATATTGCCCTCAGCCAACCTTTCGATAATCCGGG
TACGGTTGAAGTCTCCGGCCATATGGCAGACCTCAGCAAGGTCACCGCAGATGCCTTCGTCGTTGCTGGCGTCACAGATC
ACATCACCCCCTGGAAAGCCTGCTACCGCACACCGTCTCTGCTCGGCTCGAAGAATGTCGAATTCATCCTCTCTTCCAGC
GGTCACCTCCAATCCCTGATCAACCCGCCCGGTAATCCGAAGGCGAAGTATTTCCGGGGCAAGGAGATCAAACCGACGGC
GGATGAATGGGCGCTGGCCGCTGAAGAACAGGCCGGCTCCTGGTGGCCGCTCTGGGGCCAATGGCTCAAAGAACGCTCCG
GCGCCCTGAAAGCTGCACCTAAAGTGCTTGGCAACGAAGCCTTCCCCCCCATCTATGCAGCGCCAGGCCGCTACGTCTTC
AACGACTAG

Upstream 100 bases:

>100_bases
TGTCATGTTCTCTTTCAGGAAAAGAAAGGGATCATGGTTAAAACCGGCGGCTGGGTAAGGAACCCGGTGAACGTCCGGAA
GCCTTTGGGGAGGAACTACC

Downstream 100 bases:

>100_bases
GCCGTTTGAGAAAGATACGCTGATGCCCGCCACCGCCCGCAAACCCGTCATCACGATGGAAACCATCGACGGTATCACCC
TGCGCGTCGCTCATTGGCCC

Product: PHA-synthase 2

Products: NA

Alternate protein names: PHA polymerase 1; PHA synthase 1; Polyhydroxyalkanoic acid synthase 1 [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MTSPKDEIARNAAENTAALNPLLGGFNRQELLGAVGLMLRSTMTNPVTTARTAGKITAENTQILLGKSKREADKKDRRFK
DPAWEHNPFYKRGMQTYLATQEHLHAWVNEIKMGELEQARAKFVMGMITDALAPTNSLVGNPAATKRVVDSGGLSLLKGM
KNLYDDLTKNGGLPSQVDKRPFKVGENLAVSKGQVVWKNEMLELIQYAPLTEKVHKTPILIIPPQINKFYAMDLTPMTSM
VQFLLAMEQQTFVISWRNPTKKHKDWGMNDYIDSLVQASEVIRKITKSPKINVSGACSGGITTATFASLLAAADDKRINS
LTFMVCVLNPQRDDSDIGQIVSDGSLEIARKYSKSRGILKGDDLARMFAWMRPNDLIWNYVVNNYLMGEDPPPYDVLFWN
NDTTNLPAQLHSDYLDIALSQPFDNPGTVEVSGHMADLSKVTADAFVVAGVTDHITPWKACYRTPSLLGSKNVEFILSSS
GHLQSLINPPGNPKAKYFRGKEIKPTADEWALAAEEQAGSWWPLWGQWLKERSGALKAAPKVLGNEAFPPIYAAPGRYVF
ND

Sequences:

>Translated_562_residues
MTSPKDEIARNAAENTAALNPLLGGFNRQELLGAVGLMLRSTMTNPVTTARTAGKITAENTQILLGKSKREADKKDRRFK
DPAWEHNPFYKRGMQTYLATQEHLHAWVNEIKMGELEQARAKFVMGMITDALAPTNSLVGNPAATKRVVDSGGLSLLKGM
KNLYDDLTKNGGLPSQVDKRPFKVGENLAVSKGQVVWKNEMLELIQYAPLTEKVHKTPILIIPPQINKFYAMDLTPMTSM
VQFLLAMEQQTFVISWRNPTKKHKDWGMNDYIDSLVQASEVIRKITKSPKINVSGACSGGITTATFASLLAAADDKRINS
LTFMVCVLNPQRDDSDIGQIVSDGSLEIARKYSKSRGILKGDDLARMFAWMRPNDLIWNYVVNNYLMGEDPPPYDVLFWN
NDTTNLPAQLHSDYLDIALSQPFDNPGTVEVSGHMADLSKVTADAFVVAGVTDHITPWKACYRTPSLLGSKNVEFILSSS
GHLQSLINPPGNPKAKYFRGKEIKPTADEWALAAEEQAGSWWPLWGQWLKERSGALKAAPKVLGNEAFPPIYAAPGRYVF
ND
>Mature_561_residues
TSPKDEIARNAAENTAALNPLLGGFNRQELLGAVGLMLRSTMTNPVTTARTAGKITAENTQILLGKSKREADKKDRRFKD
PAWEHNPFYKRGMQTYLATQEHLHAWVNEIKMGELEQARAKFVMGMITDALAPTNSLVGNPAATKRVVDSGGLSLLKGMK
NLYDDLTKNGGLPSQVDKRPFKVGENLAVSKGQVVWKNEMLELIQYAPLTEKVHKTPILIIPPQINKFYAMDLTPMTSMV
QFLLAMEQQTFVISWRNPTKKHKDWGMNDYIDSLVQASEVIRKITKSPKINVSGACSGGITTATFASLLAAADDKRINSL
TFMVCVLNPQRDDSDIGQIVSDGSLEIARKYSKSRGILKGDDLARMFAWMRPNDLIWNYVVNNYLMGEDPPPYDVLFWNN
DTTNLPAQLHSDYLDIALSQPFDNPGTVEVSGHMADLSKVTADAFVVAGVTDHITPWKACYRTPSLLGSKNVEFILSSSG
HLQSLINPPGNPKAKYFRGKEIKPTADEWALAAEEQAGSWWPLWGQWLKERSGALKAAPKVLGNEAFPPIYAAPGRYVFN
D

Specific function: P.oleovorans accumulates poly(3-hydroxyalkanoates) after growth on medium chain length hydrocarbons. Large amounts of this polyester are synthesized when cells are grown under nitrogen- limiting conditions. When nitrogen is resupplied in the medium, the a

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011287
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 62301; Mature: 62170

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPKDEIARNAAENTAALNPLLGGFNRQELLGAVGLMLRSTMTNPVTTARTAGKITAEN
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECC
TQILLGKSKREADKKDRRFKDPAWEHNPFYKRGMQTYLATQEHLHAWVNEIKMGELEQAR
CEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKFVMGMITDALAPTNSLVGNPAATKRVVDSGGLSLLKGMKNLYDDLTKNGGLPSQVDKR
HHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
PFKVGENLAVSKGQVVWKNEMLELIQYAPLTEKVHKTPILIIPPQINKFYAMDLTPMTSM
CHHCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHCCCEEEECCCCCCEEEECCCCHHHH
VQFLLAMEQQTFVISWRNPTKKHKDWGMNDYIDSLVQASEVIRKITKSPKINVSGACSGG
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
ITTATFASLLAAADDKRINSLTFMVCVLNPQRDDSDIGQIVSDGSLEIARKYSKSRGILK
CHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCC
GDDLARMFAWMRPNDLIWNYVVNNYLMGEDPPPYDVLFWNNDTTNLPAQLHSDYLDIALS
CCHHHHHHHHCCCCHHHHHHHHHCEECCCCCCCCEEEEECCCCCCCCHHHHCHHHHHHCC
QPFDNPGTVEVSGHMADLSKVTADAFVVAGVTDHITPWKACYRTPSLLGSKNVEFILSSS
CCCCCCCEEEECCCHHHHHHHHHHHEEEECCHHCCCHHHHHHCCHHHCCCCCCEEEECCC
GHLQSLINPPGNPKAKYFRGKEIKPTADEWALAAEEQAGSWWPLWGQWLKERSGALKAAP
CCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHH
KVLGNEAFPPIYAAPGRYVFND
HHHCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
TSPKDEIARNAAENTAALNPLLGGFNRQELLGAVGLMLRSTMTNPVTTARTAGKITAEN
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECC
TQILLGKSKREADKKDRRFKDPAWEHNPFYKRGMQTYLATQEHLHAWVNEIKMGELEQAR
CEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKFVMGMITDALAPTNSLVGNPAATKRVVDSGGLSLLKGMKNLYDDLTKNGGLPSQVDKR
HHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
PFKVGENLAVSKGQVVWKNEMLELIQYAPLTEKVHKTPILIIPPQINKFYAMDLTPMTSM
CHHCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHCCCEEEECCCCCCEEEECCCCHHHH
VQFLLAMEQQTFVISWRNPTKKHKDWGMNDYIDSLVQASEVIRKITKSPKINVSGACSGG
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
ITTATFASLLAAADDKRINSLTFMVCVLNPQRDDSDIGQIVSDGSLEIARKYSKSRGILK
CHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCC
GDDLARMFAWMRPNDLIWNYVVNNYLMGEDPPPYDVLFWNNDTTNLPAQLHSDYLDIALS
CCHHHHHHHHCCCCHHHHHHHHHCEECCCCCCCCEEEEECCCCCCCCHHHHCHHHHHHCC
QPFDNPGTVEVSGHMADLSKVTADAFVVAGVTDHITPWKACYRTPSLLGSKNVEFILSSS
CCCCCCCEEEECCCHHHHHHHHHHHEEEECCHHCCCHHHHHHCCHHHCCCCCCEEEECCC
GHLQSLINPPGNPKAKYFRGKEIKPTADEWALAAEEQAGSWWPLWGQWLKERSGALKAAP
CCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHH
KVLGNEAFPPIYAAPGRYVFND
HHHCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]