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The map label for this gene is phoB [H]

Identifier: 114798462

GI number: 114798462

Start: 2104884

End: 2105570

Strand: Reverse

Name: phoB [H]

Synonym: HNE_2028

Alternate gene names: 114798462

Gene position: 2105570-2104884 (Counterclockwise)

Preceding gene: 114799041

Following gene: 114798910

Centisome position: 56.83

GC content: 63.32

Gene sequence:

>687_bases
ATGAAACCCTACGTGCTTGTCGCAGAAGACGAAAGCGCCGTCTCCGAGCTGCTGCTCTACAATCTCAAGCGGGAAGACTA
TGACGTCGCCATCGCTGCTGATGGCGAGGAAGCGCTGATGATGATCGACGAACGCATACCAGACCTGCTGCTGTTGGACT
GGATGCTGCCCAAGGTCAGCGGTATCGAAATCTGCCGCCGCGTCCGCGCCACCGGTGTCAATCCAAACCTCCCGATCATC
ATGCTCACCGCGCGCGGTGAAGAGAGCGACCGCATCCGCGGACTTGATACAGGCGCGGACGATTACGTCACCAAGCCTTT
CTCGACCACCGAGCTGATGGCCCGCGTCCGCGCGGTCCTGCGCCGCATCCGTCCCGGCCTCAAGGAAGACCGCATCACGG
TCGGCGACATCGAGATCGACCGCGTCGCCCACCGCGTCTCCCGCAACAATGAAGACATCCACCTCGGCCCCACGGAATTC
CGCCTGCTGGACTATTTCATGCAGCATCCGGGCCGCGTCTTCTCGCGCGAACAGCTCCTCGACACAGTATGGGGCTCGGA
TGTCTATGTTGAAGCCCGTACGGTGGACGTGCATGTCGGCCGCCTGCGCAAGGCGCTTCGCCAGAGCGGCGGGGAAGACC
CCATCCGCACCGTCCGCTCCGCCGGCTACGCCCTCCGCGCAGACTGA

Upstream 100 bases:

>100_bases
TTCGTCTACTACCAGGCAGTGGGAGAATACCTGTCGACCATGGAACGGCCGAAGCTGGACCAGGTCTGACGTCCATCGGA
ACAATTTACGGAGCCGAATG

Downstream 100 bases:

>100_bases
GCCTCAGAAACAACCAAAGAAAAAGGCGGAAAACCCTGATGGGTTTTCCGCCTTTTCCGTATTCAGATAATCCGATTCCA
GATTAACCAAACAGTTCGGT

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKPYVLVAEDESAVSELLLYNLKREDYDVAIAADGEEALMMIDERIPDLLLLDWMLPKVSGIEICRRVRATGVNPNLPII
MLTARGEESDRIRGLDTGADDYVTKPFSTTELMARVRAVLRRIRPGLKEDRITVGDIEIDRVAHRVSRNNEDIHLGPTEF
RLLDYFMQHPGRVFSREQLLDTVWGSDVYVEARTVDVHVGRLRKALRQSGGEDPIRTVRSAGYALRAD

Sequences:

>Translated_228_residues
MKPYVLVAEDESAVSELLLYNLKREDYDVAIAADGEEALMMIDERIPDLLLLDWMLPKVSGIEICRRVRATGVNPNLPII
MLTARGEESDRIRGLDTGADDYVTKPFSTTELMARVRAVLRRIRPGLKEDRITVGDIEIDRVAHRVSRNNEDIHLGPTEF
RLLDYFMQHPGRVFSREQLLDTVWGSDVYVEARTVDVHVGRLRKALRQSGGEDPIRTVRSAGYALRAD
>Mature_228_residues
MKPYVLVAEDESAVSELLLYNLKREDYDVAIAADGEEALMMIDERIPDLLLLDWMLPKVSGIEICRRVRATGVNPNLPII
MLTARGEESDRIRGLDTGADDYVTKPFSTTELMARVRAVLRRIRPGLKEDRITVGDIEIDRVAHRVSRNNEDIHLGPTEF
RLLDYFMQHPGRVFSREQLLDTVWGSDVYVEARTVDVHVGRLRKALRQSGGEDPIRTVRSAGYALRAD

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=50.2283105022831, Blast_Score=233, Evalue=8e-63,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=41.4096916299559, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=38.1578947368421, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=37.10407239819, Blast_Score=143, Evalue=8e-36,
Organism=Escherichia coli, GI1786911, Length=213, Percent_Identity=38.4976525821596, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=36.5957446808511, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=35.6164383561644, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=34.9775784753363, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=38.8646288209607, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=32.6271186440678, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=33.0357142857143, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=32.579185520362, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=235, Percent_Identity=33.1914893617021, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=33.7662337662338, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25887; Mature: 25887

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYVLVAEDESAVSELLLYNLKREDYDVAIAADGEEALMMIDERIPDLLLLDWMLPKVS
CCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCC
GIEICRRVRATGVNPNLPIIMLTARGEESDRIRGLDTGADDYVTKPFSTTELMARVRAVL
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHH
RRIRPGLKEDRITVGDIEIDRVAHRVSRNNEDIHLGPTEFRLLDYFMQHPGRVFSREQLL
HHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHH
DTVWGSDVYVEARTVDVHVGRLRKALRQSGGEDPIRTVRSAGYALRAD
HHHCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECC
>Mature Secondary Structure
MKPYVLVAEDESAVSELLLYNLKREDYDVAIAADGEEALMMIDERIPDLLLLDWMLPKVS
CCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCC
GIEICRRVRATGVNPNLPIIMLTARGEESDRIRGLDTGADDYVTKPFSTTELMARVRAVL
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHH
RRIRPGLKEDRITVGDIEIDRVAHRVSRNNEDIHLGPTEFRLLDYFMQHPGRVFSREQLL
HHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHH
DTVWGSDVYVEARTVDVHVGRLRKALRQSGGEDPIRTVRSAGYALRAD
HHHCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]