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The map label for this gene is phaB [H]

Identifier: 114798279

GI number: 114798279

Start: 2099728

End: 2100570

Strand: Reverse

Name: phaB [H]

Synonym: HNE_2023

Alternate gene names: 114798279

Gene position: 2100570-2099728 (Counterclockwise)

Preceding gene: 114798910

Following gene: 114800398

Centisome position: 56.7

GC content: 63.82

Gene sequence:

>843_bases
ATGCCCGCCACCGCCCGCAAACCCGTCATCACGATGGAAACCATCGACGGTATCACCCTGCGCGTCGCTCATTGGCCCGC
GACAGGGCAGGCGGGTGGCCGCCCGCTCGTCTTCTTCAACGGCATCGGCGCCAATCTGGAGCTGGCCTCCGGTCTCGGCG
GCATGTTCCCGGACCGGGAAATCCTCACCCTGGACGTCCCCGGCGTCGGCCTTAGCCCCGTCACCCAATGGCCCTACCGC
CACTGGATGCTCGCCCGCTGGATTCGCAAACTGGTCGACCGCTACGGCATCGATGAGATGGACGTCATGGGCGTCTCCTG
GGGCGGCGCGCTCGCCCAGCAATTCGCTTTCCAGTATCGCAGCCGCGTCAAACGTCTCATCCTGTGCGCCACCACAGCCG
GCGTCACCATGATCCCCGGCCGCCCGGCCTCGCTCTCGAAAATGGTCGATACGCGCCGCTATTCAGATCCCGAATTCATG
CGCGAAAACTTCGCCAAACTCTATGGCGACGCGGCCGATGAATCCGCAGGCGGACATATCGACAATCTCATGCCGCCAGA
CCCCAAAGGCTATCTCTACCAGATCCTCGCCTTCGCGGGCTGGTCGAGCCTTCCCTTCATCCGTTTCCTGAAGATGCCAA
CGCTGGTCATCATGGGCGATGCAGACTCCATCGTGCCCGTCGCCAACGGCCACATCCTGAACTTCGCCCTGCCAGATTCT
CGGCTGCGCATCATTAAGGGCGGCGGCCACCTCTTCCTCGTCACCCGCGCCGAGGAAACCGGCGCGCTGATCCGCGAATT
CATGGATGAGGAAAACGCGGCGCCGCTGAGCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTGCACCTAAAGTGCTTGGCAACGAAGCCTTCCCCCCCATCTATGCAGCGCCAGGCCGCTACGTCTTCAACGACTAGGC
CGTTTGAGAAAGATACGCTG

Downstream 100 bases:

>100_bases
GCCGCGCTCAGTCGTTCATCAGCCGCTCGGCCGCAATCCCCACGGCGCCATAATCCACCTTGCCCGTCCCCAGCACGGGG
ATCTCGGCCACACGGAACAC

Product: poly(3-hydroxyalkanoate) depolymerase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MPATARKPVITMETIDGITLRVAHWPATGQAGGRPLVFFNGIGANLELASGLGGMFPDREILTLDVPGVGLSPVTQWPYR
HWMLARWIRKLVDRYGIDEMDVMGVSWGGALAQQFAFQYRSRVKRLILCATTAGVTMIPGRPASLSKMVDTRRYSDPEFM
RENFAKLYGDAADESAGGHIDNLMPPDPKGYLYQILAFAGWSSLPFIRFLKMPTLVIMGDADSIVPVANGHILNFALPDS
RLRIIKGGGHLFLVTRAEETGALIREFMDEENAAPLSNAA

Sequences:

>Translated_280_residues
MPATARKPVITMETIDGITLRVAHWPATGQAGGRPLVFFNGIGANLELASGLGGMFPDREILTLDVPGVGLSPVTQWPYR
HWMLARWIRKLVDRYGIDEMDVMGVSWGGALAQQFAFQYRSRVKRLILCATTAGVTMIPGRPASLSKMVDTRRYSDPEFM
RENFAKLYGDAADESAGGHIDNLMPPDPKGYLYQILAFAGWSSLPFIRFLKMPTLVIMGDADSIVPVANGHILNFALPDS
RLRIIKGGGHLFLVTRAEETGALIREFMDEENAAPLSNAA
>Mature_279_residues
PATARKPVITMETIDGITLRVAHWPATGQAGGRPLVFFNGIGANLELASGLGGMFPDREILTLDVPGVGLSPVTQWPYRH
WMLARWIRKLVDRYGIDEMDVMGVSWGGALAQQFAFQYRSRVKRLILCATTAGVTMIPGRPASLSKMVDTRRYSDPEFMR
ENFAKLYGDAADESAGGHIDNLMPPDPKGYLYQILAFAGWSSLPFIRFLKMPTLVIMGDADSIVPVANGHILNFALPDSR
LRIIKGGGHLFLVTRAEETGALIREFMDEENAAPLSNAA

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30750; Mature: 30619

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATARKPVITMETIDGITLRVAHWPATGQAGGRPLVFFNGIGANLELASGLGGMFPDRE
CCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCE
ILTLDVPGVGLSPVTQWPYRHWMLARWIRKLVDRYGIDEMDVMGVSWGGALAQQFAFQYR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
SRVKRLILCATTAGVTMIPGRPASLSKMVDTRRYSDPEFMRENFAKLYGDAADESAGGHI
HHHHHEEEEEECCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DNLMPPDPKGYLYQILAFAGWSSLPFIRFLKMPTLVIMGDADSIVPVANGHILNFALPDS
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCEEEECCCEEEEEECCCC
RLRIIKGGGHLFLVTRAEETGALIREFMDEENAAPLSNAA
CEEEEECCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PATARKPVITMETIDGITLRVAHWPATGQAGGRPLVFFNGIGANLELASGLGGMFPDRE
CCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCE
ILTLDVPGVGLSPVTQWPYRHWMLARWIRKLVDRYGIDEMDVMGVSWGGALAQQFAFQYR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
SRVKRLILCATTAGVTMIPGRPASLSKMVDTRRYSDPEFMRENFAKLYGDAADESAGGHI
HHHHHEEEEEECCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DNLMPPDPKGYLYQILAFAGWSSLPFIRFLKMPTLVIMGDADSIVPVANGHILNFALPDS
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCEEEECCCEEEEEECCCC
RLRIIKGGGHLFLVTRAEETGALIREFMDEENAAPLSNAA
CEEEEECCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]