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The map label for this gene is otsB [H]

Identifier: 114798428

GI number: 114798428

Start: 1608637

End: 1609338

Strand: Direct

Name: otsB [H]

Synonym: HNE_1576

Alternate gene names: 114798428

Gene position: 1608637-1609338 (Clockwise)

Preceding gene: 114798991

Following gene: 114799613

Centisome position: 43.42

GC content: 57.55

Gene sequence:

>702_bases
TTGCTTGATCCATCCGCCGCGCTGTTCCTTGATTTTGACGGAACGATGGCGCCACTCCAGTCTGATCCGGATACGGTGTT
TCTTCCAGAGGGAGGCGCCGAGATTTTGCTGAGAACAGCTGAGAATCTTCATGGCGCTCTGGTGATCCTCAGTGGGCGGG
ATATTCGTGATCTTGCCTCGCGCATTCCTGATGGACTTTGGCGCGCTGGCGGTCATGGTGCTTACGTTTGCCCTCCAGGA
CATGCGCCGCCGGGCGAACTTCCCGAAGCGCCGGAAAATCTCGTTGCGGAGATTTCTGCCTTGATCGAAGAGATCCCTGG
GGTGCGCATGGAATCAAAAGGACCGGTGCTGGCGGTACATTATCGCGAGATACCGGAAGCGGCGGGCCCCCTTGAAGCAG
GTTTGACAACACTGCTTGAAGCATTTCCGGAATACACGCTTCAATCTGGAAAGATGGTGATGGAAGCAAGACCAGCCAGT
GCGCACAAAGGAAAATGCGTTCGTGAGCTGATGAAGCAGGCGCCCTTTTCGGGCCGGGCGCCCATAATGGTTGGAGATGA
TACAACTGATGAAGATGCCATGCGCGCAAGTCTCGACTTCGGAGGCACTGCCATCAAGGTAGGCGCTGGGGCGACGGCTG
CGCCCTACCGGCTGGCCGACCCGGCAGCGGTTTGGGACTGGCTCAAGAGAGGACTGACATGA

Upstream 100 bases:

>100_bases
GCGTGGTGGCGGGACCTGTTCCTGAACGACCTTCGCGCCAAAATCTGATCGCTCGCTTTCGACCTTTAAATCCATCTGCC
AGGAATAAATGACCGCTCCT

Downstream 100 bases:

>100_bases
ACAATCTGGAACTTGGGATCATTGGCAACGGAACGATTGCTGCCTTGATTGATGAAGCGGGTGAAATCGGCTGGCTTTGC
TTGCCGAGGCTCGATGGAGA

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLDPSAALFLDFDGTMAPLQSDPDTVFLPEGGAEILLRTAENLHGALVILSGRDIRDLASRIPDGLWRAGGHGAYVCPPG
HAPPGELPEAPENLVAEISALIEEIPGVRMESKGPVLAVHYREIPEAAGPLEAGLTTLLEAFPEYTLQSGKMVMEARPAS
AHKGKCVRELMKQAPFSGRAPIMVGDDTTDEDAMRASLDFGGTAIKVGAGATAAPYRLADPAAVWDWLKRGLT

Sequences:

>Translated_233_residues
MLDPSAALFLDFDGTMAPLQSDPDTVFLPEGGAEILLRTAENLHGALVILSGRDIRDLASRIPDGLWRAGGHGAYVCPPG
HAPPGELPEAPENLVAEISALIEEIPGVRMESKGPVLAVHYREIPEAAGPLEAGLTTLLEAFPEYTLQSGKMVMEARPAS
AHKGKCVRELMKQAPFSGRAPIMVGDDTTDEDAMRASLDFGGTAIKVGAGATAAPYRLADPAAVWDWLKRGLT
>Mature_233_residues
MLDPSAALFLDFDGTMAPLQSDPDTVFLPEGGAEILLRTAENLHGALVILSGRDIRDLASRIPDGLWRAGGHGAYVCPPG
HAPPGELPEAPENLVAEISALIEEIPGVRMESKGPVLAVHYREIPEAAGPLEAGLTTLLEAFPEYTLQSGKMVMEARPAS
AHKGKCVRELMKQAPFSGRAPIMVGDDTTDEDAMRASLDFGGTAIKVGAGATAAPYRLADPAAVWDWLKRGLT

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=231, Percent_Identity=35.4978354978355, Blast_Score=137, Evalue=5e-34,
Organism=Drosophila melanogaster, GI221473368, Length=236, Percent_Identity=31.3559322033898, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI20129307, Length=236, Percent_Identity=31.3559322033898, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582490, Length=236, Percent_Identity=31.3559322033898, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19920676, Length=238, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 24645; Mature: 24645

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPSAALFLDFDGTMAPLQSDPDTVFLPEGGAEILLRTAENLHGALVILSGRDIRDLAS
CCCCCEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHH
RIPDGLWRAGGHGAYVCPPGHAPPGELPEAPENLVAEISALIEEIPGVRMESKGPVLAVH
HCCHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEE
YREIPEAAGPLEAGLTTLLEAFPEYTLQSGKMVMEARPASAHKGKCVRELMKQAPFSGRA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCC
PIMVGDDTTDEDAMRASLDFGGTAIKVGAGATAAPYRLADPAAVWDWLKRGLT
CEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLDPSAALFLDFDGTMAPLQSDPDTVFLPEGGAEILLRTAENLHGALVILSGRDIRDLAS
CCCCCEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHH
RIPDGLWRAGGHGAYVCPPGHAPPGELPEAPENLVAEISALIEEIPGVRMESKGPVLAVH
HCCHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEE
YREIPEAAGPLEAGLTTLLEAFPEYTLQSGKMVMEARPASAHKGKCVRELMKQAPFSGRA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCC
PIMVGDDTTDEDAMRASLDFGGTAIKVGAGATAAPYRLADPAAVWDWLKRGLT
CEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]