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The map label for this gene is otsA [H]

Identifier: 114798991

GI number: 114798991

Start: 1607217

End: 1608584

Strand: Direct

Name: otsA [H]

Synonym: HNE_1575

Alternate gene names: 114798991

Gene position: 1607217-1608584 (Clockwise)

Preceding gene: 114797699

Following gene: 114798428

Centisome position: 43.38

GC content: 56.73

Gene sequence:

>1368_bases
TTGGGACGCCTGGTAGCAATATCCAACAGAACGGCCGCCGATGCCGGCGCGCAGGCGGGCGGGCTTGCTGTGGCCGTATG
GGACGCCCTCAAAGCGACAGGCGGATTATGGGCAGGCTGGTCCGGCGATATTGTCAGCGACTCCCCAGGCAGCAAACAAT
TTGAATCAGAAGACGGCGTTTCTTTCCTTCTGACCGACATGACAGAAGACGAGCATGACGGCTTTTACCTGCGCTATTCC
AACAGCACGCTTTGGCCAATATTTCACTATCGGGTAGATCTGGCGACGTTCGATCGCGGTGCCTTTGACCATTATCTCGC
GGTAAACGAGCGATTGGCAAACCTCACAATTGAGCATCTGCGCGAACATGACATGGTTTGGGTGCATGACTACCATTTCC
TCCCGATGGCCGATGCGTTGCGCCGGGCCGGATGGAAGGGGCGCACCGGATTTTTCCTGCACATACCGTTTCCCACACCA
GACATGTTCCGCGCTCTTCCAGAGCATAGCTGGCTGGCACGCGCGCTTTGCCAGTTTGATCTGATCGGCTTCCAGTCCGA
ATCCGACCGCACGAATTTCGAACGTTATCTGGTCGACAGCCTGGATGCAGATGCTTCTGAAGCGCCGATTTATACTGTCG
CAGGCCGGAGAGTGCGTGTGGGTGTCTATCCCATTGGAATTGATGCTGCCGCTTTTGAAGAAGCCGCTCGAAGCCCTGCG
GCCGGGGACGCGGCTGCCCGCATCGGACGTTTCCTGGGAAATCGCGATCTCATCATCGGTGTGGACCGGATGGATTATTC
CAAAGGGCTGCCTCAACGATTTGAGGCCGTTGGCGCATTTTTTGACAACCATCCGGAGCTGCATGGCAAGGTGTCGGTAA
CGCAGATTGCGACCCCCTCACGAACCAAAGTGGAAGAGTATCGCCAGCTGCGCTATGAACTCGATCAGCTCGTGGGACGT
ATCAATGGAGATTATGGCGATCTCGACTGGATACCATTGCGCTATCTGGCGCGAAGCTATTCCAGAGATGAATTGGCCGG
CCTCTTCCGCATCGCACGCGTAGGGTTGGTTACGCCGCTTCGGGACGGGATGAACCTCGTGGCAAAGGAATACGTGATGG
CGCAGGACCCGGAAGATCCCGGCGTTCTTATACTTTCAGAATTCGCTGGGGCCGCGGAACAACTTCACGAAGCGCTGATC
GTAAATCCCCATGACCGCTCGACATTGGCGGAGACCATCCGGATCGCGCTGACTATGCCTCTTGAGGAACGAAAACGGCG
ATGGGAGGCCCTCCGCGATGTTGTAAGGCGGACAGATATTGCGTGGTGGCGGGACCTGTTCCTGAACGACCTTCGCGCCA
AAATCTGA

Upstream 100 bases:

>100_bases
CTGCCTACAAGGGATAACCCATGCGGACGGGTGGACATGATGGAGCGAAGCTGACGTAATCAAACCTGATCAGATAAAGC
AGTGAGGAATTGGAGCAAAT

Downstream 100 bases:

>100_bases
TCGCTCGCTTTCGACCTTTAAATCCATCTGCCAGGAATAAATGACCGCTCCTTTGCTTGATCCATCCGCCGCGCTGTTCC
TTGATTTTGACGGAACGATG

Product: UDP-forming alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MGRLVAISNRTAADAGAQAGGLAVAVWDALKATGGLWAGWSGDIVSDSPGSKQFESEDGVSFLLTDMTEDEHDGFYLRYS
NSTLWPIFHYRVDLATFDRGAFDHYLAVNERLANLTIEHLREHDMVWVHDYHFLPMADALRRAGWKGRTGFFLHIPFPTP
DMFRALPEHSWLARALCQFDLIGFQSESDRTNFERYLVDSLDADASEAPIYTVAGRRVRVGVYPIGIDAAAFEEAARSPA
AGDAAARIGRFLGNRDLIIGVDRMDYSKGLPQRFEAVGAFFDNHPELHGKVSVTQIATPSRTKVEEYRQLRYELDQLVGR
INGDYGDLDWIPLRYLARSYSRDELAGLFRIARVGLVTPLRDGMNLVAKEYVMAQDPEDPGVLILSEFAGAAEQLHEALI
VNPHDRSTLAETIRIALTMPLEERKRRWEALRDVVRRTDIAWWRDLFLNDLRAKI

Sequences:

>Translated_455_residues
MGRLVAISNRTAADAGAQAGGLAVAVWDALKATGGLWAGWSGDIVSDSPGSKQFESEDGVSFLLTDMTEDEHDGFYLRYS
NSTLWPIFHYRVDLATFDRGAFDHYLAVNERLANLTIEHLREHDMVWVHDYHFLPMADALRRAGWKGRTGFFLHIPFPTP
DMFRALPEHSWLARALCQFDLIGFQSESDRTNFERYLVDSLDADASEAPIYTVAGRRVRVGVYPIGIDAAAFEEAARSPA
AGDAAARIGRFLGNRDLIIGVDRMDYSKGLPQRFEAVGAFFDNHPELHGKVSVTQIATPSRTKVEEYRQLRYELDQLVGR
INGDYGDLDWIPLRYLARSYSRDELAGLFRIARVGLVTPLRDGMNLVAKEYVMAQDPEDPGVLILSEFAGAAEQLHEALI
VNPHDRSTLAETIRIALTMPLEERKRRWEALRDVVRRTDIAWWRDLFLNDLRAKI
>Mature_454_residues
GRLVAISNRTAADAGAQAGGLAVAVWDALKATGGLWAGWSGDIVSDSPGSKQFESEDGVSFLLTDMTEDEHDGFYLRYSN
STLWPIFHYRVDLATFDRGAFDHYLAVNERLANLTIEHLREHDMVWVHDYHFLPMADALRRAGWKGRTGFFLHIPFPTPD
MFRALPEHSWLARALCQFDLIGFQSESDRTNFERYLVDSLDADASEAPIYTVAGRRVRVGVYPIGIDAAAFEEAARSPAA
GDAAARIGRFLGNRDLIIGVDRMDYSKGLPQRFEAVGAFFDNHPELHGKVSVTQIATPSRTKVEEYRQLRYELDQLVGRI
NGDYGDLDWIPLRYLARSYSRDELAGLFRIARVGLVTPLRDGMNLVAKEYVMAQDPEDPGVLILSEFAGAAEQLHEALIV
NPHDRSTLAETIRIALTMPLEERKRRWEALRDVVRRTDIAWWRDLFLNDLRAKI

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=454, Percent_Identity=47.136563876652, Blast_Score=411, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71987012, Length=424, Percent_Identity=28.3018867924528, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI115533558, Length=423, Percent_Identity=27.6595744680851, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115533560, Length=423, Percent_Identity=27.6595744680851, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=34.2553191489362, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6320279, Length=401, Percent_Identity=30.1745635910224, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6323917, Length=445, Percent_Identity=27.4157303370787, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323537, Length=395, Percent_Identity=27.3417721518987, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19920676, Length=465, Percent_Identity=38.7096774193548, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 51344; Mature: 51212

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLVAISNRTAADAGAQAGGLAVAVWDALKATGGLWAGWSGDIVSDSPGSKQFESEDGV
CCCEEEECCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SFLLTDMTEDEHDGFYLRYSNSTLWPIFHYRVDLATFDRGAFDHYLAVNERLANLTIEHL
EEEEECCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
REHDMVWVHDYHFLPMADALRRAGWKGRTGFFLHIPFPTPDMFRALPEHSWLARALCQFD
HHCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHH
LIGFQSESDRTNFERYLVDSLDADASEAPIYTVAGRRVRVGVYPIGIDAAAFEEAARSPA
HCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEEECCCHHHHHHHHCCCC
AGDAAARIGRFLGNRDLIIGVDRMDYSKGLPQRFEAVGAFFDNHPELHGKVSVTQIATPS
CCHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
RTKVEEYRQLRYELDQLVGRINGDYGDLDWIPLRYLARSYSRDELAGLFRIARVGLVTPL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHH
RDGMNLVAKEYVMAQDPEDPGVLILSEFAGAAEQLHEALIVNPHDRSTLAETIRIALTMP
HHHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHEEECCCCHHHHHHHHHHEEECC
LEERKRRWEALRDVVRRTDIAWWRDLFLNDLRAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRLVAISNRTAADAGAQAGGLAVAVWDALKATGGLWAGWSGDIVSDSPGSKQFESEDGV
CCEEEECCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SFLLTDMTEDEHDGFYLRYSNSTLWPIFHYRVDLATFDRGAFDHYLAVNERLANLTIEHL
EEEEECCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
REHDMVWVHDYHFLPMADALRRAGWKGRTGFFLHIPFPTPDMFRALPEHSWLARALCQFD
HHCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHH
LIGFQSESDRTNFERYLVDSLDADASEAPIYTVAGRRVRVGVYPIGIDAAAFEEAARSPA
HCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEEECCCHHHHHHHHCCCC
AGDAAARIGRFLGNRDLIIGVDRMDYSKGLPQRFEAVGAFFDNHPELHGKVSVTQIATPS
CCHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
RTKVEEYRQLRYELDQLVGRINGDYGDLDWIPLRYLARSYSRDELAGLFRIARVGLVTPL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHH
RDGMNLVAKEYVMAQDPEDPGVLILSEFAGAAEQLHEALIVNPHDRSTLAETIRIALTMP
HHHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHEEECCCCHHHHHHHHHHEEECC
LEERKRRWEALRDVVRRTDIAWWRDLFLNDLRAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA