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The map label for this gene is lipA [H]

Identifier: 114800398

GI number: 114800398

Start: 2096999

End: 2098054

Strand: Reverse

Name: lipA [H]

Synonym: HNE_2021

Alternate gene names: 114800398

Gene position: 2098054-2096999 (Counterclockwise)

Preceding gene: 114798279

Following gene: 114799654

Centisome position: 56.63

GC content: 63.64

Gene sequence:

>1056_bases
TTGCCAGTGCGCCCCACCTCCTGCATGGGTCGTCTGCAGACGCTGACGCTTGATGGAATCGCCTCGAAGGTCCATTTCCT
CCCCATGGCCAATCTCATAGACACCCGTTCCGGCGCCCCGCGCCCGCGCCATCCCGAAAAGCAGGCCCGGCCAGACACGC
CCGTTCTGCGCAAGCCGGACTGGATTCGCGTCAAGGCGCCCACCTCGCAGGGCTATAACGAGACCCGCAATATCGTGAAG
TCGAAGGGCCTCGTCACTGTCTGCGAGGAGGCCGGCTGCCCCAATATCGGCGAGTGCTGGGACAAGAAACACGCCACCAT
GATGATCCTGGGCGAGGTCTGCACCCGCGCCTGCTCCTTCTGCAACGTCGCCACCGGCAAGCCAAAGCCCGGCGTGGAAG
AAGACGAGCCCGCCCGCGTGGCCGAGGCCGTCGCCGAAATGGGCCTCTCCCATGTCGTCATCACATCCGTTGACCGCGAC
GATCTGGAAGACGGCGGCGCCATGCACTTCGTCAATACGATCAACGCCATCCGCGAACGCTCGCCCGGCACGACGATTGA
AATCCTCACGCCGGATTTCCTGCGCAAGGATGGCTGGGAAAACCGCGTCATCGATGCGCGGCCCGACGTGTTCAATCACA
ATCTGGAAACCGTCCCGCGCCTCTATCTCACCATCCGCCCCGGCGCGCGCTATTTCCATTCCCTCCGCCTGCTGCAAAAG
GTGAAGGACCGCGACCCCGCCCAGTTCACCAAATCCGGGCTGATGGTGGGCCTGGGTGAAACCAAGGAAGAGGTGATGCA
GGTGATGGACGACATGCGCTCTGCCGGCGTCGACTTCCTCACCATCGGCCAATATCTTCAGCCCACGCGCAAACACGCCG
CGATTGACCGCTTCGTCACGCCCGAAGAGTTCAAGGCGTATGAAGAAATCGCCCGCGCCAAAGGCTTCCTCATGGTCTCG
GCCAGCCCGCTCACGCGCTCCAGCCACCATGCGGGCGAAGACTTCGCCCAGCTGCGCGCCGCGCGCGAAGCCTTTCTCGC
CGCCCGCAAAGCGTAA

Upstream 100 bases:

>100_bases
TGGCTGGATCGATTGCTGCATAGGTTTCCGCCCTGAATCATACCGGCTTGAGCCGAATTTTGTAATATTCTGTCATGTTG
CGTCATATCTGCAACAGATA

Downstream 100 bases:

>100_bases
GGCATGCCGCGCTTCACCAAGACCCTGCGTGTGCCTTACGGGCCGCCGCAATGCTTTGCGCTGGTGTCAGACATCGCCCG
CTATCCTGATTTCATAAAAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPVRPTSCMGRLQTLTLDGIASKVHFLPMANLIDTRSGAPRPRHPEKQARPDTPVLRKPDWIRVKAPTSQGYNETRNIVK
SKGLVTVCEEAGCPNIGECWDKKHATMMILGEVCTRACSFCNVATGKPKPGVEEDEPARVAEAVAEMGLSHVVITSVDRD
DLEDGGAMHFVNTINAIRERSPGTTIEILTPDFLRKDGWENRVIDARPDVFNHNLETVPRLYLTIRPGARYFHSLRLLQK
VKDRDPAQFTKSGLMVGLGETKEEVMQVMDDMRSAGVDFLTIGQYLQPTRKHAAIDRFVTPEEFKAYEEIARAKGFLMVS
ASPLTRSSHHAGEDFAQLRAAREAFLAARKA

Sequences:

>Translated_351_residues
MPVRPTSCMGRLQTLTLDGIASKVHFLPMANLIDTRSGAPRPRHPEKQARPDTPVLRKPDWIRVKAPTSQGYNETRNIVK
SKGLVTVCEEAGCPNIGECWDKKHATMMILGEVCTRACSFCNVATGKPKPGVEEDEPARVAEAVAEMGLSHVVITSVDRD
DLEDGGAMHFVNTINAIRERSPGTTIEILTPDFLRKDGWENRVIDARPDVFNHNLETVPRLYLTIRPGARYFHSLRLLQK
VKDRDPAQFTKSGLMVGLGETKEEVMQVMDDMRSAGVDFLTIGQYLQPTRKHAAIDRFVTPEEFKAYEEIARAKGFLMVS
ASPLTRSSHHAGEDFAQLRAAREAFLAARKA
>Mature_350_residues
PVRPTSCMGRLQTLTLDGIASKVHFLPMANLIDTRSGAPRPRHPEKQARPDTPVLRKPDWIRVKAPTSQGYNETRNIVKS
KGLVTVCEEAGCPNIGECWDKKHATMMILGEVCTRACSFCNVATGKPKPGVEEDEPARVAEAVAEMGLSHVVITSVDRDD
LEDGGAMHFVNTINAIRERSPGTTIEILTPDFLRKDGWENRVIDARPDVFNHNLETVPRLYLTIRPGARYFHSLRLLQKV
KDRDPAQFTKSGLMVGLGETKEEVMQVMDDMRSAGVDFLTIGQYLQPTRKHAAIDRFVTPEEFKAYEEIARAKGFLMVSA
SPLTRSSHHAGEDFAQLRAAREAFLAARKA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=43.6860068259386, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=44.5344129554656, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=51.063829787234, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=41.522491349481, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=300, Percent_Identity=42, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=43.006993006993, Blast_Score=251, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 39075; Mature: 38944

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRPTSCMGRLQTLTLDGIASKVHFLPMANLIDTRSGAPRPRHPEKQARPDTPVLRKPD
CCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCC
WIRVKAPTSQGYNETRNIVKSKGLVTVCEEAGCPNIGECWDKKHATMMILGEVCTRACSF
EEEEECCCCCCHHHHHHHHHHCCCEEEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
CNVATGKPKPGVEEDEPARVAEAVAEMGLSHVVITSVDRDDLEDGGAMHFVNTINAIRER
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHHHC
SPGTTIEILTPDFLRKDGWENRVIDARPDVFNHNLETVPRLYLTIRPGARYFHSLRLLQK
CCCCEEEEECHHHHHCCCCCCCCCCCCCCHHCCCHHHCCEEEEEECCCHHHHHHHHHHHH
VKDRDPAQFTKSGLMVGLGETKEEVMQVMDDMRSAGVDFLTIGQYLQPTRKHAAIDRFVT
HHCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCC
PEEFKAYEEIARAKGFLMVSASPLTRSSHHAGEDFAQLRAAREAFLAARKA
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVRPTSCMGRLQTLTLDGIASKVHFLPMANLIDTRSGAPRPRHPEKQARPDTPVLRKPD
CCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCC
WIRVKAPTSQGYNETRNIVKSKGLVTVCEEAGCPNIGECWDKKHATMMILGEVCTRACSF
EEEEECCCCCCHHHHHHHHHHCCCEEEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
CNVATGKPKPGVEEDEPARVAEAVAEMGLSHVVITSVDRDDLEDGGAMHFVNTINAIRER
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHHHC
SPGTTIEILTPDFLRKDGWENRVIDARPDVFNHNLETVPRLYLTIRPGARYFHSLRLLQK
CCCCEEEEECHHHHHCCCCCCCCCCCCCCHHCCCHHHCCEEEEEECCCHHHHHHHHHHHH
VKDRDPAQFTKSGLMVGLGETKEEVMQVMDDMRSAGVDFLTIGQYLQPTRKHAAIDRFVT
HHCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCC
PEEFKAYEEIARAKGFLMVSASPLTRSSHHAGEDFAQLRAAREAFLAARKA
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA