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The map label for this gene is rluE [H]

Identifier: 114798241

GI number: 114798241

Start: 1409910

End: 1410446

Strand: Reverse

Name: rluE [H]

Synonym: HNE_1378

Alternate gene names: 114798241

Gene position: 1410446-1409910 (Counterclockwise)

Preceding gene: 114798946

Following gene: 114797476

Centisome position: 38.07

GC content: 64.8

Gene sequence:

>537_bases
ATGACCCTCATCCTGTTCAACAAGCCCTATGGCGTCCTCAGCCAGTTCACCGATGAGGGCAGCGGCAAGCGCACGCTCAA
GGAGTTTATCGCCCTTCCCGGCGTCTATCCCGCCGGCCGGCTGGACTTTGACAGCGAGGGCCTCCTCATCCTCACCGATG
ATGGCCGCGAACAGGCCCGCATCGCCGATCCGAAACACAAAATCCCGAAGACCTACCTTGCCCAGGTCGAAGGGATACCG
TCAGAGGAAGCTCTCCAGGCGCTGCGCAAGGGCGTGGACCTCAAAGACGGTCGCACCCGCCCGGCCACGGCGCGCCTCGC
CGCAGAACCCGAAAACCTCTGGCCGCGCGATCCGCCGATCCGCTACCGCAAATCCGTGCCCGACACGTGGATCGAACTCA
CCATCACCGAAGGGCGCAACCGTCAGGTGCGCCGCATGACGGCGGCCGCGGGCCACCCGACGCTGCGTCTGATCCGCCTA
CGGATTGGAGATTGGGACCTCGAAAATCTTCCGCCCGGAGCCTGGCGCAAAGCCTGA

Upstream 100 bases:

>100_bases
CGTAAAGGCGCGGTATGTAAACATGAGCGTTACCAATTCTGCATGATCGCGCGCTCGCAGGGCTTTACGGCCCGCCACCG
GGCAAGGCAAAGGGGCGCTC

Downstream 100 bases:

>100_bases
AGATTCCCGAAAAATTCAAAAATTTACAGCCTCGCCGGTTAACATCGCGGCCCACCCGGCCTAGGTGCCACCTCAGGCAA
CTGAAACCAGACAAGGGGAC

Product: RNA pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MTLILFNKPYGVLSQFTDEGSGKRTLKEFIALPGVYPAGRLDFDSEGLLILTDDGREQARIADPKHKIPKTYLAQVEGIP
SEEALQALRKGVDLKDGRTRPATARLAAEPENLWPRDPPIRYRKSVPDTWIELTITEGRNRQVRRMTAAAGHPTLRLIRL
RIGDWDLENLPPGAWRKA

Sequences:

>Translated_178_residues
MTLILFNKPYGVLSQFTDEGSGKRTLKEFIALPGVYPAGRLDFDSEGLLILTDDGREQARIADPKHKIPKTYLAQVEGIP
SEEALQALRKGVDLKDGRTRPATARLAAEPENLWPRDPPIRYRKSVPDTWIELTITEGRNRQVRRMTAAAGHPTLRLIRL
RIGDWDLENLPPGAWRKA
>Mature_177_residues
TLILFNKPYGVLSQFTDEGSGKRTLKEFIALPGVYPAGRLDFDSEGLLILTDDGREQARIADPKHKIPKTYLAQVEGIPS
EEALQALRKGVDLKDGRTRPATARLAAEPENLWPRDPPIRYRKSVPDTWIELTITEGRNRQVRRMTAAAGHPTLRLIRLR
IGDWDLENLPPGAWRKA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=61.9318181818182, Blast_Score=219, Evalue=8e-59,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=33.5195530726257, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787524, Length=179, Percent_Identity=33.5195530726257, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1790453, Length=177, Percent_Identity=30.5084745762712, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20075; Mature: 19944

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLILFNKPYGVLSQFTDEGSGKRTLKEFIALPGVYPAGRLDFDSEGLLILTDDGREQAR
CEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
IADPKHKIPKTYLAQVEGIPSEEALQALRKGVDLKDGRTRPATARLAAEPENLWPRDPPI
CCCCHHHCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
RYRKSVPDTWIELTITEGRNRQVRRMTAAAGHPTLRLIRLRIGDWDLENLPPGAWRKA
HHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLILFNKPYGVLSQFTDEGSGKRTLKEFIALPGVYPAGRLDFDSEGLLILTDDGREQAR
EEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
IADPKHKIPKTYLAQVEGIPSEEALQALRKGVDLKDGRTRPATARLAAEPENLWPRDPPI
CCCCHHHCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
RYRKSVPDTWIELTITEGRNRQVRRMTAAAGHPTLRLIRLRIGDWDLENLPPGAWRKA
HHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]