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The map label for this gene is fixJ [H]

Identifier: 114798134

GI number: 114798134

Start: 1255725

End: 1256342

Strand: Direct

Name: fixJ [H]

Synonym: HNE_1235

Alternate gene names: 114798134

Gene position: 1255725-1256342 (Clockwise)

Preceding gene: 114797394

Following gene: 114800503

Centisome position: 33.89

GC content: 58.58

Gene sequence:

>618_bases
ATGACTGACAACCGCCTTATCCATCTCGTCGATGACGACGAAGCCATCCGCCACTCCGCGAGCTTCATGCTGCGCCATGC
CGGCTTTCGCGTGAAAACCTATGTCGATGGCGTCGCCTTTCTGGAGCAGGCGGGCGAAGCCGAAAAAGGCTGCATCCTGC
TGGATGTCCAGATGCCGAAGATGGACGGCCTGCAGGTGCAGGAAGCGCTGAATGAACGCGGCGTGGCCATGCCGGTCATC
GTCCTGACGGGGCATGGCGATGTCGCCGTTGCCGTCCGTGCGATGAAAGCAGGCGCGGTGGATTTTGTCGAAAAGCCTTA
TGCAAAGCAGACCCTGGTCGACGCGCTCACCCGCGCGTTCGAGCGTCTGGAATCGCGCCGCAAGGAAGACGTGCTCGCCG
ATGAAGCGCGCGGCCTGATCGAAAATCTTACCGCCCGCGAGAAAGAAGTTCTTCATGGCCTGGTCGATGGCCAGACCAAC
AAGGAAATCGCCATCTCGCTGGATATTTCTCCGCGCACGGTTGAAATTCATCGAGCTAATGTGATGGAAAAGATGGGGGC
ATCCAACCTGTCCACGGTGCTTCGTATTGCCTTCGCCGCAGGCATTGGCCTTGAATAA

Upstream 100 bases:

>100_bases
TCGTGGAAGCGCATGGCGGTGTCATCCGCGCCATTACGCCGCCAGAGGGGGGAACCTGTTTCCGGTTCACACTGAGGAAA
GATGCAGGTAAATCAGGATC

Downstream 100 bases:

>100_bases
TCAGCAGCTTTAGGGGCTTCTACTTACAGACGGCCCAGGAGATTCCTGCGCAAGGTGCAGCCTCCCGGAATGCCGGAGTG
TCCCTTGAGCGCCATCGCAA

Product: DNA-binding response regulator FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTDNRLIHLVDDDEAIRHSASFMLRHAGFRVKTYVDGVAFLEQAGEAEKGCILLDVQMPKMDGLQVQEALNERGVAMPVI
VLTGHGDVAVAVRAMKAGAVDFVEKPYAKQTLVDALTRAFERLESRRKEDVLADEARGLIENLTAREKEVLHGLVDGQTN
KEIAISLDISPRTVEIHRANVMEKMGASNLSTVLRIAFAAGIGLE

Sequences:

>Translated_205_residues
MTDNRLIHLVDDDEAIRHSASFMLRHAGFRVKTYVDGVAFLEQAGEAEKGCILLDVQMPKMDGLQVQEALNERGVAMPVI
VLTGHGDVAVAVRAMKAGAVDFVEKPYAKQTLVDALTRAFERLESRRKEDVLADEARGLIENLTAREKEVLHGLVDGQTN
KEIAISLDISPRTVEIHRANVMEKMGASNLSTVLRIAFAAGIGLE
>Mature_204_residues
TDNRLIHLVDDDEAIRHSASFMLRHAGFRVKTYVDGVAFLEQAGEAEKGCILLDVQMPKMDGLQVQEALNERGVAMPVIV
LTGHGDVAVAVRAMKAGAVDFVEKPYAKQTLVDALTRAFERLESRRKEDVLADEARGLIENLTAREKEVLHGLVDGQTNK
EIAISLDISPRTVEIHRANVMEKMGASNLSTVLRIAFAAGIGLE

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=31.7073170731707, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790102, Length=145, Percent_Identity=35.8620689655172, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788521, Length=157, Percent_Identity=31.2101910828025, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=34.4, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788191, Length=127, Percent_Identity=33.0708661417323, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786599, Length=137, Percent_Identity=29.9270072992701, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788712, Length=158, Percent_Identity=25.9493670886076, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22460; Mature: 22329

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNRLIHLVDDDEAIRHSASFMLRHAGFRVKTYVDGVAFLEQAGEAEKGCILLDVQMPK
CCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEECCC
MDGLQVQEALNERGVAMPVIVLTGHGDVAVAVRAMKAGAVDFVEKPYAKQTLVDALTRAF
CCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERLESRRKEDVLADEARGLIENLTAREKEVLHGLVDGQTNKEIAISLDISPRTVEIHRAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHH
VMEKMGASNLSTVLRIAFAAGIGLE
HHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDNRLIHLVDDDEAIRHSASFMLRHAGFRVKTYVDGVAFLEQAGEAEKGCILLDVQMPK
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEECCC
MDGLQVQEALNERGVAMPVIVLTGHGDVAVAVRAMKAGAVDFVEKPYAKQTLVDALTRAF
CCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERLESRRKEDVLADEARGLIENLTAREKEVLHGLVDGQTNKEIAISLDISPRTVEIHRAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHH
VMEKMGASNLSTVLRIAFAAGIGLE
HHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]