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The map label for this gene is fixL [H]

Identifier: 114797394

GI number: 114797394

Start: 1254562

End: 1255728

Strand: Direct

Name: fixL [H]

Synonym: HNE_1234

Alternate gene names: 114797394

Gene position: 1254562-1255728 (Clockwise)

Preceding gene: 114800164

Following gene: 114798134

Centisome position: 33.86

GC content: 62.38

Gene sequence:

>1167_bases
ATGAGCGACACGCCGAAATCGACTCAGCCGCCCGTGAACCTCCTCGACGGGGCGACCCTTCAGCTTCATCTCGCCTCGAT
CCTGGCCTCGGTGCCCGATGGCATGGTCGTCATTGACGAGTCCGGAAAGGTCATGGCCTTCAGCCGGGCGGCCGAGGCAT
TGTTCGGGTTCACCGCAGAGGAAGTCATTGGCCAGCCGGTCAACATGTTGATGGCCGGCCGCGACAAGGTGAACCACGAC
AACTATATCGGCAACTATCTGCGCACCGGCGAGCGCCAGATCATCGGCAAGGGCCGCGTTGTCATTGCCTCACGCGCAGA
TGGTACGCAGTTTCCCATCGACCTCAAGATCGGCGAAGCGCGCATCGGTGATCATTTTCTCTTCACCGCCTTCATCCGTG
ACCTGACAGAGCAGCAGCGCTCCGAGCTGCGCATGCAGGAAATGCAGTCAGAGCTGGTCCACTTCTCCCGCCTCAGCGCG
GTGGGCACGATGGCGTCAGCGCTCGCCCATGAGCTCAACCAGCCGCTGACGGCGGTGGCAAATTACCTGGAAGCCAGCCG
CGACCTGCTCGATTCGCCCGACCCGGAGATGAAAGACGTTCTGCGCGAGGCGCTGACCGAAGCAGCCCGTCAGGCCGTGC
GCGCGGGCGAAATCGTCCGCAAGCTGCGCTCCTATGTCTCCCGTGGGGAGGTGGACGCCCGCCCGCTCGGCCTCGGCCCA
CTCCTTGCCGACGCCATCGCCCTGTCCAAACTCTCGCGTGACCTTGCCGACATCCCGATCAAGCTCGATCAGGAGGAGGC
CACCGATCATGTCATGGGCGACCCCATTCAGATCCAGCAGGTCGTCATAAACCTGATCCGCAACGCCATGGACGCCCTCC
CCAACACGCAGGACGCTCGCATCATGGTGCGCGTCTATTCTGCGGAGGAGCCGGGATTTGTTGCCATCGAAGTGTGCGAT
AACGGCCCTGGCCTGACGGACGACCTGCGGGAGAACATCTTCAAGCCCTTCGCCACCACCAAATCGCAGGGCATGGGCCT
CGGCCTCTCCATCTGTCAGACCATCGTGGAAGCGCATGGCGGTGTCATCCGCGCCATTACGCCGCCAGAGGGGGGAACCT
GTTTCCGGTTCACACTGAGGAAAGATGCAGGTAAATCAGGATCATGA

Upstream 100 bases:

>100_bases
CACCTGACGCCGCTGCGCCGCCGTTGAAAATAAGGCGAACGCCTTGCGCCGCAGCGCAGCTGAGGGCAGGGATAACGCCT
TACCGGATGGACCTTGACGT

Downstream 100 bases:

>100_bases
CTGACAACCGCCTTATCCATCTCGTCGATGACGACGAAGCCATCCGCCACTCCGCGAGCTTCATGCTGCGCCATGCCGGC
TTTCGCGTGAAAACCTATGT

Product: sensory box sensor histidine kinase FixL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSDTPKSTQPPVNLLDGATLQLHLASILASVPDGMVVIDESGKVMAFSRAAEALFGFTAEEVIGQPVNMLMAGRDKVNHD
NYIGNYLRTGERQIIGKGRVVIASRADGTQFPIDLKIGEARIGDHFLFTAFIRDLTEQQRSELRMQEMQSELVHFSRLSA
VGTMASALAHELNQPLTAVANYLEASRDLLDSPDPEMKDVLREALTEAARQAVRAGEIVRKLRSYVSRGEVDARPLGLGP
LLADAIALSKLSRDLADIPIKLDQEEATDHVMGDPIQIQQVVINLIRNAMDALPNTQDARIMVRVYSAEEPGFVAIEVCD
NGPGLTDDLRENIFKPFATTKSQGMGLGLSICQTIVEAHGGVIRAITPPEGGTCFRFTLRKDAGKSGS

Sequences:

>Translated_388_residues
MSDTPKSTQPPVNLLDGATLQLHLASILASVPDGMVVIDESGKVMAFSRAAEALFGFTAEEVIGQPVNMLMAGRDKVNHD
NYIGNYLRTGERQIIGKGRVVIASRADGTQFPIDLKIGEARIGDHFLFTAFIRDLTEQQRSELRMQEMQSELVHFSRLSA
VGTMASALAHELNQPLTAVANYLEASRDLLDSPDPEMKDVLREALTEAARQAVRAGEIVRKLRSYVSRGEVDARPLGLGP
LLADAIALSKLSRDLADIPIKLDQEEATDHVMGDPIQIQQVVINLIRNAMDALPNTQDARIMVRVYSAEEPGFVAIEVCD
NGPGLTDDLRENIFKPFATTKSQGMGLGLSICQTIVEAHGGVIRAITPPEGGTCFRFTLRKDAGKSGS
>Mature_387_residues
SDTPKSTQPPVNLLDGATLQLHLASILASVPDGMVVIDESGKVMAFSRAAEALFGFTAEEVIGQPVNMLMAGRDKVNHDN
YIGNYLRTGERQIIGKGRVVIASRADGTQFPIDLKIGEARIGDHFLFTAFIRDLTEQQRSELRMQEMQSELVHFSRLSAV
GTMASALAHELNQPLTAVANYLEASRDLLDSPDPEMKDVLREALTEAARQAVRAGEIVRKLRSYVSRGEVDARPLGLGPL
LADAIALSKLSRDLADIPIKLDQEEATDHVMGDPIQIQQVVINLIRNAMDALPNTQDARIMVRVYSAEEPGFVAIEVCDN
GPGLTDDLRENIFKPFATTKSQGMGLGLSICQTIVEAHGGVIRAITPPEGGTCFRFTLRKDAGKSGS

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=26.6106442577031, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=28.1632653061224, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786912, Length=223, Percent_Identity=30.0448430493274, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790300, Length=374, Percent_Identity=24.0641711229947, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081816, Length=156, Percent_Identity=34.6153846153846, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI48994928, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=109, Percent_Identity=33.0275229357798, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790346, Length=108, Percent_Identity=32.4074074074074, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42131; Mature: 42000

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPKSTQPPVNLLDGATLQLHLASILASVPDGMVVIDESGKVMAFSRAAEALFGFTAE
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHCCCHH
EVIGQPVNMLMAGRDKVNHDNYIGNYLRTGERQIIGKGRVVIASRADGTQFPIDLKIGEA
HHCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHEECCCCEEEEECCCCCCCCEEEEECCC
RIGDHFLFTAFIRDLTEQQRSELRMQEMQSELVHFSRLSAVGTMASALAHELNQPLTAVA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NYLEASRDLLDSPDPEMKDVLREALTEAARQAVRAGEIVRKLRSYVSRGEVDARPLGLGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LLADAIALSKLSRDLADIPIKLDQEEATDHVMGDPIQIQQVVINLIRNAMDALPNTQDAR
HHHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
IMVRVYSAEEPGFVAIEVCDNGPGLTDDLRENIFKPFATTKSQGMGLGLSICQTIVEAHG
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
GVIRAITPPEGGTCFRFTLRKDAGKSGS
CEEEEECCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SDTPKSTQPPVNLLDGATLQLHLASILASVPDGMVVIDESGKVMAFSRAAEALFGFTAE
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHCCCHH
EVIGQPVNMLMAGRDKVNHDNYIGNYLRTGERQIIGKGRVVIASRADGTQFPIDLKIGEA
HHCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHEECCCCEEEEECCCCCCCCEEEEECCC
RIGDHFLFTAFIRDLTEQQRSELRMQEMQSELVHFSRLSAVGTMASALAHELNQPLTAVA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NYLEASRDLLDSPDPEMKDVLREALTEAARQAVRAGEIVRKLRSYVSRGEVDARPLGLGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LLADAIALSKLSRDLADIPIKLDQEEATDHVMGDPIQIQQVVINLIRNAMDALPNTQDAR
HHHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
IMVRVYSAEEPGFVAIEVCDNGPGLTDDLRENIFKPFATTKSQGMGLGLSICQTIVEAHG
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
GVIRAITPPEGGTCFRFTLRKDAGKSGS
CEEEEECCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]