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The map label for this gene is ppnK

Identifier: 114797751

GI number: 114797751

Start: 2137753

End: 2138520

Strand: Direct

Name: ppnK

Synonym: HNE_2064

Alternate gene names: 114797751

Gene position: 2137753-2138520 (Clockwise)

Preceding gene: 114800329

Following gene: 114798002

Centisome position: 57.7

GC content: 64.32

Gene sequence:

>768_bases
ATGACCCCGCTGCGTATTGCTTTTTATGCTTCCAAACGGCCAGAGGCGCAGCAGGTGCTGCCGCAGCTGCGCGACAAGTA
CGGCCATTATTCCGAGGAAGAGGCCGACGTGATCGTCGCCCTGGGCGGAGACGGCGCCATGCTGGACACGCTTCGCAGGC
GCTTTGACGATGGCAAGCCGGTCTATGGCATGCATCTGGGCACCGTGGGTTTCCTGATGAATGACTTTCATGCCGATGGC
CTGCCCGAGCGAATCGAGGCGGCCGAGCGGGCCACGCTGTCACCCCTGCGGATGCAGGCGACGGACCTGGACGGCACGGT
TCACCGGGCGATGGCGATCAACGAGATTTCGCTGCTGCGCCAGACGGCGCAATCGGCCCGGCTGAAGATCATCGTGGATG
GGCGGGTGCGGATGGAGGAGCTGGTGTGCGACGGGCTGATGGTCGCGACGCCCGCCGGATCGACCGCGTATAATCTCTCC
GCCCATGGCCCGATCCTGCCGATCGGGGCAAAGCTGCTGGCGCTGACGCCGGTGAGCGCGTTCCGGCCCCGGCGCTGGCG
CGGCGCGCTGCTGAAGGCTGAAGCAAGGGTAGACATTGAAGTGGTGGCGCCTGACCGGCGGCCGGTGTCTGCGTCTGCGG
ACAATGAAGAAGTGCGCAATATTGCCAAGGTGACGGTGGAAACTGACCCAGCGCGCACCCTGAAGGTGCTGTTTGACCCC
GGCCATGCGCTGGATGAGCGAATTTTGCGGGAGCAGTTTGCTTTCTGA

Upstream 100 bases:

>100_bases
GCCGCGAGGGAAGGCCTGCCCCGCTTGGCAGACCCCATTCCCGGCCCCTGTTCGGGGCCCGCCTTGACGGGTGGGGCAAA
ACATTCCCACATGCCGCCGC

Downstream 100 bases:

>100_bases
AGCAGTTTCAACGGCTCGCGTCAGCGTAATGTTAAATCTTGTTGGTTAATCCTGCCCCTCAAGAAACGAGGGTGGAGTGA
TGCTGGGTAAATTCCTGAAA

Product: ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTPLRIAFYASKRPEAQQVLPQLRDKYGHYSEEEADVIVALGGDGAMLDTLRRRFDDGKPVYGMHLGTVGFLMNDFHADG
LPERIEAAERATLSPLRMQATDLDGTVHRAMAINEISLLRQTAQSARLKIIVDGRVRMEELVCDGLMVATPAGSTAYNLS
AHGPILPIGAKLLALTPVSAFRPRRWRGALLKAEARVDIEVVAPDRRPVSASADNEEVRNIAKVTVETDPARTLKVLFDP
GHALDERILREQFAF

Sequences:

>Translated_255_residues
MTPLRIAFYASKRPEAQQVLPQLRDKYGHYSEEEADVIVALGGDGAMLDTLRRRFDDGKPVYGMHLGTVGFLMNDFHADG
LPERIEAAERATLSPLRMQATDLDGTVHRAMAINEISLLRQTAQSARLKIIVDGRVRMEELVCDGLMVATPAGSTAYNLS
AHGPILPIGAKLLALTPVSAFRPRRWRGALLKAEARVDIEVVAPDRRPVSASADNEEVRNIAKVTVETDPARTLKVLFDP
GHALDERILREQFAF
>Mature_254_residues
TPLRIAFYASKRPEAQQVLPQLRDKYGHYSEEEADVIVALGGDGAMLDTLRRRFDDGKPVYGMHLGTVGFLMNDFHADGL
PERIEAAERATLSPLRMQATDLDGTVHRAMAINEISLLRQTAQSARLKIIVDGRVRMEELVCDGLMVATPAGSTAYNLSA
HGPILPIGAKLLALTPVSAFRPRRWRGALLKAEARVDIEVVAPDRRPVSASADNEEVRNIAKVTVETDPARTLKVLFDPG
HALDERILREQFAF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=156, Percent_Identity=32.6923076923077, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=172, Percent_Identity=29.6511627906977, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322509, Length=210, Percent_Identity=29.0476190476191, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_HYPNA (Q0C0I1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760762.1
- ProteinModelPortal:   Q0C0I1
- STRING:   Q0C0I1
- GeneID:   4288288
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2064
- NMPDR:   fig|228405.5.peg.1990
- TIGR:   HNE_2064
- eggNOG:   COG0061
- HOGENOM:   HBG294544
- OMA:   VIHPLLM
- BioCyc:   HNEP81032:HNE_2064-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 28134; Mature: 28003

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLRIAFYASKRPEAQQVLPQLRDKYGHYSEEEADVIVALGGDGAMLDTLRRRFDDGKP
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
VYGMHLGTVGFLMNDFHADGLPERIEAAERATLSPLRMQATDLDGTVHRAMAINEISLLR
EEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
QTAQSARLKIIVDGRVRMEELVCDGLMVATPAGSTAYNLSAHGPILPIGAKLLALTPVSA
HHHHHCEEEEEECCCHHHHHHHHCCEEEECCCCCCEEECCCCCCEEECCCEEEEECCHHH
FRPRRWRGALLKAEARVDIEVVAPDRRPVSASADNEEVRNIAKVTVETDPARTLKVLFDP
HCCCCHHCCEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCEEEEEEECC
GHALDERILREQFAF
CHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPLRIAFYASKRPEAQQVLPQLRDKYGHYSEEEADVIVALGGDGAMLDTLRRRFDDGKP
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
VYGMHLGTVGFLMNDFHADGLPERIEAAERATLSPLRMQATDLDGTVHRAMAINEISLLR
EEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHH
QTAQSARLKIIVDGRVRMEELVCDGLMVATPAGSTAYNLSAHGPILPIGAKLLALTPVSA
HHHHHCEEEEEECCCHHHHHHHHCCEEEECCCCCCEEECCCCCCEEECCCEEEEECCHHH
FRPRRWRGALLKAEARVDIEVVAPDRRPVSASADNEEVRNIAKVTVETDPARTLKVLFDP
HCCCCHHCCEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCEEEEEEECC
GHALDERILREQFAF
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA