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The map label for this gene is cobD [H]

Identifier: 114797305

GI number: 114797305

Start: 1553199

End: 1554149

Strand: Direct

Name: cobD [H]

Synonym: HNE_1519

Alternate gene names: 114797305

Gene position: 1553199-1554149 (Clockwise)

Preceding gene: 114797990

Following gene: 114798819

Centisome position: 41.92

GC content: 59.94

Gene sequence:

>951_bases
ATGACACCCGCTCTGCTCATGCTGGCTTCCTGGGCGGTCGAGGCGGTGTTCGGCTGGCCTAACTGGCTTTATTGGCGCGT
GCGCCACCCAGTGGTCTGGCTGGGAGTCTTGATCGGCTGGCTAGAACAGGGCCTCAACAGACAGGCATTTTCGCATTGGG
TGCGTTACATGTTCGGTGGGTTTACCAGCTTGCTCGCGGTGACGCTTGCCACTTGGTTGGCAGTGCTTTTGGTGCACAGT
CTTCCTGATGGATGGACAGGCTATGTGGTCCAGGCGGCTATTGCGTCCAGCCTTCTTGCATCGCGCAGTCTCTACCAGCA
TGTAGCCGCGGTTGCCGTGCCGCTTTCGGTTGGCGACCAGTCCGCAGCCCGCAAGGCGGTGTCGCTGATCGTCGGCCGCG
ATCCCAGCCGGCTGGATGAGGCCGGCATCGCCCGCGCGTCGATTGAAAGCCTCGCTGAAAATGCCTCAGACGGTGTCATC
GCACCACTTTTTTGGGGCGCGCTTTTTGGAATTCCCGGTCTCGTCGCCTACAAAACCATAAATACTCTGGACTCGATCAT
TGGCCATCGCAATGAACGGTACGAAGCATTTGGCGGCTTCGCGGCTCGGCTTGATGATGTCGCCAATCTGATCCCAGCGC
GCATGACGGGTCTGTTCGTTGTAATCGCTTCTATGCGTTTCTCAGCATTGGCAATAGTGGTGAGAGACGCGCGAAAACAT
CGGTCGCCGAACGCTGGCTGGCCTGAAGCGGCGATGGCTGGCGCGCTCGGCATCCGCCTCTCGGGTCCGCGTATATACGG
TGACAGGTGCAGCGAGGAGCCATGGCTCAATCCCGAAGCGCGAGACCCCGGCGCCTCTGATATTCAAAGGGCGCTGCGGC
TTTACATACTGGCGCTGGCGCTGGTCGGTCTCACACTGATTGGCGTTGTTCTGTTGGGGGCGTCCAGGTGA

Upstream 100 bases:

>100_bases
CCCATGGCGCGCGCTCGGCGCATGGCCTCATCGAGGGCACCTATCTGCACGGCGCGTTCTCGAACGATGCGTTTCGCCGT
GGTTGGCAACCGGCGACGTG

Downstream 100 bases:

>100_bases
AACCGGATCTTGTTCATGGCGGCGCGCTAGACCGGATGCGTGCGGCCTTCCCTGATGCGCCTGAGCCCTGGATCGACCTT
TCGACCGGGATCAATCCATG

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTPALLMLASWAVEAVFGWPNWLYWRVRHPVVWLGVLIGWLEQGLNRQAFSHWVRYMFGGFTSLLAVTLATWLAVLLVHS
LPDGWTGYVVQAAIASSLLASRSLYQHVAAVAVPLSVGDQSAARKAVSLIVGRDPSRLDEAGIARASIESLAENASDGVI
APLFWGALFGIPGLVAYKTINTLDSIIGHRNERYEAFGGFAARLDDVANLIPARMTGLFVVIASMRFSALAIVVRDARKH
RSPNAGWPEAAMAGALGIRLSGPRIYGDRCSEEPWLNPEARDPGASDIQRALRLYILALALVGLTLIGVVLLGASR

Sequences:

>Translated_316_residues
MTPALLMLASWAVEAVFGWPNWLYWRVRHPVVWLGVLIGWLEQGLNRQAFSHWVRYMFGGFTSLLAVTLATWLAVLLVHS
LPDGWTGYVVQAAIASSLLASRSLYQHVAAVAVPLSVGDQSAARKAVSLIVGRDPSRLDEAGIARASIESLAENASDGVI
APLFWGALFGIPGLVAYKTINTLDSIIGHRNERYEAFGGFAARLDDVANLIPARMTGLFVVIASMRFSALAIVVRDARKH
RSPNAGWPEAAMAGALGIRLSGPRIYGDRCSEEPWLNPEARDPGASDIQRALRLYILALALVGLTLIGVVLLGASR
>Mature_315_residues
TPALLMLASWAVEAVFGWPNWLYWRVRHPVVWLGVLIGWLEQGLNRQAFSHWVRYMFGGFTSLLAVTLATWLAVLLVHSL
PDGWTGYVVQAAIASSLLASRSLYQHVAAVAVPLSVGDQSAARKAVSLIVGRDPSRLDEAGIARASIESLAENASDGVIA
PLFWGALFGIPGLVAYKTINTLDSIIGHRNERYEAFGGFAARLDDVANLIPARMTGLFVVIASMRFSALAIVVRDARKHR
SPNAGWPEAAMAGALGIRLSGPRIYGDRCSEEPWLNPEARDPGASDIQRALRLYILALALVGLTLIGVVLLGASR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34161; Mature: 34030

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPALLMLASWAVEAVFGWPNWLYWRVRHPVVWLGVLIGWLEQGLNRQAFSHWVRYMFGG
CCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FTSLLAVTLATWLAVLLVHSLPDGWTGYVVQAAIASSLLASRSLYQHVAAVAVPLSVGDQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SAARKAVSLIVGRDPSRLDEAGIARASIESLAENASDGVIAPLFWGALFGIPGLVAYKTI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHH
NTLDSIIGHRNERYEAFGGFAARLDDVANLIPARMTGLFVVIASMRFSALAIVVRDARKH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RSPNAGWPEAAMAGALGIRLSGPRIYGDRCSEEPWLNPEARDPGASDIQRALRLYILALA
CCCCCCCCHHHHHHHHCEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LVGLTLIGVVLLGASR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPALLMLASWAVEAVFGWPNWLYWRVRHPVVWLGVLIGWLEQGLNRQAFSHWVRYMFGG
CHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FTSLLAVTLATWLAVLLVHSLPDGWTGYVVQAAIASSLLASRSLYQHVAAVAVPLSVGDQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SAARKAVSLIVGRDPSRLDEAGIARASIESLAENASDGVIAPLFWGALFGIPGLVAYKTI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHH
NTLDSIIGHRNERYEAFGGFAARLDDVANLIPARMTGLFVVIASMRFSALAIVVRDARKH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RSPNAGWPEAAMAGALGIRLSGPRIYGDRCSEEPWLNPEARDPGASDIQRALRLYILALA
CCCCCCCCHHHHHHHHCEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LVGLTLIGVVLLGASR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]