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The map label for this gene is rfbB [H]

Identifier: 114332356

GI number: 114332356

Start: 2514002

End: 2515063

Strand: Direct

Name: rfbB [H]

Synonym: Neut_2400

Alternate gene names: 114332356

Gene position: 2514002-2515063 (Clockwise)

Preceding gene: 114332355

Following gene: 114332357

Centisome position: 94.47

GC content: 45.76

Gene sequence:

>1062_bases
ATGTCAACGATCTTGATTACCGGCGGCGCCGGTTTTATAGGAACAAATTTCATACTGGATTGGGTCAAGAATACGGATGA
GAATATCGTTAACCTTGACAAATTGACCTACGCTGGAAATCTGCATAATCTTGTTTCACTCACCAATAATCCTCAGCATA
TCTTTGTGCAGGGGGACATCTGCGATAGTGAGAGAATATCCACTCTCCTTGAACGCTATCAACCTCGTGCCATTATTCAT
ATGGCGGCAGAAAGCCATGTGGATCGCTCTATCCATGGGCCCGAATATTTTATTCAGACCAACATCACCGGCACATTCCG
GTTGCTGGAAGCAGCTCGTCATTACTGGGGAGATTTGTCTGTCTCACGCAAGGAAAATTTCCGATTCCTGCATGTTTCTA
CAGATGAGGTTTTCGGCACATTAACCAAGGATGCTCCTGCATTTACTGAAAATCACTGCTTTCAACCTAATAGCCCTTAT
TCCGCCAGCAAGGCCTCCAGTGACTGTCTTGTCCGTGCCTACCACCATACTTATGGCCTGCCGGTACTGACTACACATTG
TTCCAACAACTATGGGGCATATCAGTTTCCTGAGAAACTGATTCCTCTGATGATCGTCAATGCTCTAGCAGGCAAACCGC
TGCCTGTTTATGGGGATGGCCAGCAGATCCGCGACTGGCTGTATGTAAATGATCACTGCCAAGCTATTCGTGTTGTTCTG
GAAAAAGGACAACCAGGGGAAACCTACAATATTGGAGGCTGGAATGAAAAAAGTAATCTTGAAATCATACACACCATTTG
CGACGTACTTCAGCAATTTCAACAAAAAGATTATCGCGCACTAATTACTCATGTGGCTGATCGCCCTGGCCACGATCGTC
GCTATGCGATCAACGCACATAAAATAGAACGAGAACTTGGCTGGAGGCCGCTGGAAACATTTGAGACTGGTATCCGTAAA
ACTATTCAATGGTATCTGGAAAATCATGACTGGGTTGCAGAAGTGCAATCGGGCGACTACCGGAAATGGGTTGAAATTAA
CTATACCGGGCGATCACAGTGA

Upstream 100 bases:

>100_bases
AATCGGCATCGACTGTAACTTTCAGGCACCAATGGGAAGAGCCGGGCTAGTGGTAACATGGCGCCCCGATCAGACCAATC
AATCTTCAATTCACTGATAT

Downstream 100 bases:

>100_bases
AAATATTGCTCTTTGGTAAAAATGGCCAGGTCGGCTGGGAGCTACAGCGTAGCCTGGCTCCGTTGGGCAAATTGATTGCG
CTGGATCGACGGGATTTACA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSTILITGGAGFIGTNFILDWVKNTDENIVNLDKLTYAGNLHNLVSLTNNPQHIFVQGDICDSERISTLLERYQPRAIIH
MAAESHVDRSIHGPEYFIQTNITGTFRLLEAARHYWGDLSVSRKENFRFLHVSTDEVFGTLTKDAPAFTENHCFQPNSPY
SASKASSDCLVRAYHHTYGLPVLTTHCSNNYGAYQFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVNDHCQAIRVVL
EKGQPGETYNIGGWNEKSNLEIIHTICDVLQQFQQKDYRALITHVADRPGHDRRYAINAHKIERELGWRPLETFETGIRK
TIQWYLENHDWVAEVQSGDYRKWVEINYTGRSQ

Sequences:

>Translated_353_residues
MSTILITGGAGFIGTNFILDWVKNTDENIVNLDKLTYAGNLHNLVSLTNNPQHIFVQGDICDSERISTLLERYQPRAIIH
MAAESHVDRSIHGPEYFIQTNITGTFRLLEAARHYWGDLSVSRKENFRFLHVSTDEVFGTLTKDAPAFTENHCFQPNSPY
SASKASSDCLVRAYHHTYGLPVLTTHCSNNYGAYQFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVNDHCQAIRVVL
EKGQPGETYNIGGWNEKSNLEIIHTICDVLQQFQQKDYRALITHVADRPGHDRRYAINAHKIERELGWRPLETFETGIRK
TIQWYLENHDWVAEVQSGDYRKWVEINYTGRSQ
>Mature_352_residues
STILITGGAGFIGTNFILDWVKNTDENIVNLDKLTYAGNLHNLVSLTNNPQHIFVQGDICDSERISTLLERYQPRAIIHM
AAESHVDRSIHGPEYFIQTNITGTFRLLEAARHYWGDLSVSRKENFRFLHVSTDEVFGTLTKDAPAFTENHCFQPNSPYS
ASKASSDCLVRAYHHTYGLPVLTTHCSNNYGAYQFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVNDHCQAIRVVLE
KGQPGETYNIGGWNEKSNLEIIHTICDVLQQFQQKDYRALITHVADRPGHDRRYAINAHKIERELGWRPLETFETGIRKT
IQWYLENHDWVAEVQSGDYRKWVEINYTGRSQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=39.1566265060241, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=23.5294117647059, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=24.3553008595989, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=57.5418994413408, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=57.9250720461095, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=25.7971014492754, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788366, Length=203, Percent_Identity=31.0344827586207, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=35.9882005899705, Blast_Score=200, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=32.8358208955224, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI133901786, Length=286, Percent_Identity=25.5244755244755, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=282, Percent_Identity=24.468085106383, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=286, Percent_Identity=25.5244755244755, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=286, Percent_Identity=25.5244755244755, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=286, Percent_Identity=25.5244755244755, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=286, Percent_Identity=25.5244755244755, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=21.4067278287462, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=25.4189944134078, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=24.7252747252747, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24158427, Length=280, Percent_Identity=26.4285714285714, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=24.1590214067278, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40451; Mature: 40320

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILITGGAGFIGTNFILDWVKNTDENIVNLDKLTYAGNLHNLVSLTNNPQHIFVQGDI
CCEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHEEECCCCHHHEEECCCCCEEEEECCC
CDSERISTLLERYQPRAIIHMAAESHVDRSIHGPEYFIQTNITGTFRLLEAARHYWGDLS
CCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCE
VSRKENFRFLHVSTDEVFGTLTKDAPAFTENHCFQPNSPYSASKASSDCLVRAYHHTYGL
EECCCCEEEEEEEHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCC
PVLTTHCSNNYGAYQFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVNDHCQAIRVVL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEECHHHHHHHHHH
EKGQPGETYNIGGWNEKSNLEIIHTICDVLQQFQQKDYRALITHVADRPGHDRRYAINAH
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
KIERELGWRPLETFETGIRKTIQWYLENHDWVAEVQSGDYRKWVEINYTGRSQ
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
STILITGGAGFIGTNFILDWVKNTDENIVNLDKLTYAGNLHNLVSLTNNPQHIFVQGDI
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHEEECCCCHHHEEECCCCCEEEEECCC
CDSERISTLLERYQPRAIIHMAAESHVDRSIHGPEYFIQTNITGTFRLLEAARHYWGDLS
CCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCE
VSRKENFRFLHVSTDEVFGTLTKDAPAFTENHCFQPNSPYSASKASSDCLVRAYHHTYGL
EECCCCEEEEEEEHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCC
PVLTTHCSNNYGAYQFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVNDHCQAIRVVL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEECHHHHHHHHHH
EKGQPGETYNIGGWNEKSNLEIIHTICDVLQQFQQKDYRALITHVADRPGHDRRYAINAH
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
KIERELGWRPLETFETGIRKTIQWYLENHDWVAEVQSGDYRKWVEINYTGRSQ
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]