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The map label for this gene is tilS [H]

Identifier: 114332355

GI number: 114332355

Start: 2512742

End: 2513998

Strand: Direct

Name: tilS [H]

Synonym: Neut_2399

Alternate gene names: 114332355

Gene position: 2512742-2513998 (Clockwise)

Preceding gene: 114332354

Following gene: 114332356

Centisome position: 94.43

GC content: 45.66

Gene sequence:

>1257_bases
GTGGTATTGCTACACCTGCTTATAAAGTTTTCCAAATTAATGCCACTGGATGTATCTGCAATTCATGTTGAACATGGTAT
CAGCACATATTCAGGTCAATGGAGTGCTTTCTGCCAAACACTTTGCGACAGTGTTGCTATTCCCTTATCAATTCATCGAT
TAAAGATCAGCAAGCAGCCACAGGAAAGCCTGGAAGCGGTTGCCAGAGAAGCTCGTTATCAAATATTCAGGCAAATTCAG
GCAGATTATGTCTTGTTGGCGCAACATCAGGATGATCAGGTTGAGACATTGCTACTGCAGCTATTGCGTGGTGCAGGTGT
TAAAGGGCTCAGTGCTATGCCGACAGTCAGATTATTTGAATCAGGCAAAGCCATCAAATTACTCAGACCCTTACTTAATG
TTCCGCGCTCAGAAATCATGAAATATGCAAAGTTGCATGGTTTGTCATGGGTAACCGATGAAAGCAATCTGGATGTTTCT
TATGACCGTAATTTTTTACGGCACCGGATTTTACCTTTAATAGAACAGCGCTACCCCTCTTACCGCAAGATATTGTCCAG
ATCTGCCCGACATCTTGGTGAAGCAGCTCATTTACTGGATGAACTGGCAAAAATAGATGCTGAAAATACTATGATCGTAG
ATAAATTGAGCTTGCGAGGGCTGCGTGAGCTTGATCTAGCCCGTGCTGGGAATTTGCTGCGTTATTTGTTAGCACAGCAA
ATGGTGCGGTTGCCAAGTTCCGCAAAACTGGAAGAGATTTTGCGTCAGCTGCATAACATACAAGCTGATACCCATTTTCA
GTTCGAGGTAGATGCTTTGGAAATTCGCTGCTACCGTGGACTAATCGAATTTCTGCCTGCTGATTCCCTGTCGGAATCAA
TATCGCCTGTTATCTGGCAAGGCCAGCAACGCTTGGCTATTGAACCATTGAAAGGCATACTTGAGTTTACTCGGCAAGAC
AACGCGGGAATTGACCTGGCCAAACTGGATCAGCAGATTGTTACAATCCGTTCACGATCCGGTGGAGAACGATTCCAGCC
AGATTGTAAACGTCCTCGTCGCAGCCTGAAAAAAATTCTACAGGAAGCCTCACTTCCTCCGTGGAAGCGCAACACATTGC
CGTTACTTTTCTGTGGGGATCAGCTTGTCTGGGTAGCTGGAATCGGCATCGACTGTAACTTTCAGGCACCAATGGGAAGA
GCCGGGCTAGTGGTAACATGGCGCCCCGATCAGACCAATCAATCTTCAATTCACTGA

Upstream 100 bases:

>100_bases
CCCGATTAATGATATAACCAGCTGTTTTTTCCATAATCTGAATGCACAAATCAGGCCTGGTGATTGTCTGGTAGTTGCTC
TGAGTGGTGGCGTAGATTCT

Downstream 100 bases:

>100_bases
TATATGTCAACGATCTTGATTACCGGCGGCGCCGGTTTTATAGGAACAAATTTCATACTGGATTGGGTCAAGAATACGGA
TGAGAATATCGTTAACCTTG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MVLLHLLIKFSKLMPLDVSAIHVEHGISTYSGQWSAFCQTLCDSVAIPLSIHRLKISKQPQESLEAVAREARYQIFRQIQ
ADYVLLAQHQDDQVETLLLQLLRGAGVKGLSAMPTVRLFESGKAIKLLRPLLNVPRSEIMKYAKLHGLSWVTDESNLDVS
YDRNFLRHRILPLIEQRYPSYRKILSRSARHLGEAAHLLDELAKIDAENTMIVDKLSLRGLRELDLARAGNLLRYLLAQQ
MVRLPSSAKLEEILRQLHNIQADTHFQFEVDALEIRCYRGLIEFLPADSLSESISPVIWQGQQRLAIEPLKGILEFTRQD
NAGIDLAKLDQQIVTIRSRSGGERFQPDCKRPRRSLKKILQEASLPPWKRNTLPLLFCGDQLVWVAGIGIDCNFQAPMGR
AGLVVTWRPDQTNQSSIH

Sequences:

>Translated_418_residues
MVLLHLLIKFSKLMPLDVSAIHVEHGISTYSGQWSAFCQTLCDSVAIPLSIHRLKISKQPQESLEAVAREARYQIFRQIQ
ADYVLLAQHQDDQVETLLLQLLRGAGVKGLSAMPTVRLFESGKAIKLLRPLLNVPRSEIMKYAKLHGLSWVTDESNLDVS
YDRNFLRHRILPLIEQRYPSYRKILSRSARHLGEAAHLLDELAKIDAENTMIVDKLSLRGLRELDLARAGNLLRYLLAQQ
MVRLPSSAKLEEILRQLHNIQADTHFQFEVDALEIRCYRGLIEFLPADSLSESISPVIWQGQQRLAIEPLKGILEFTRQD
NAGIDLAKLDQQIVTIRSRSGGERFQPDCKRPRRSLKKILQEASLPPWKRNTLPLLFCGDQLVWVAGIGIDCNFQAPMGR
AGLVVTWRPDQTNQSSIH
>Mature_418_residues
MVLLHLLIKFSKLMPLDVSAIHVEHGISTYSGQWSAFCQTLCDSVAIPLSIHRLKISKQPQESLEAVAREARYQIFRQIQ
ADYVLLAQHQDDQVETLLLQLLRGAGVKGLSAMPTVRLFESGKAIKLLRPLLNVPRSEIMKYAKLHGLSWVTDESNLDVS
YDRNFLRHRILPLIEQRYPSYRKILSRSARHLGEAAHLLDELAKIDAENTMIVDKLSLRGLRELDLARAGNLLRYLLAQQ
MVRLPSSAKLEEILRQLHNIQADTHFQFEVDALEIRCYRGLIEFLPADSLSESISPVIWQGQQRLAIEPLKGILEFTRQD
NAGIDLAKLDQQIVTIRSRSGGERFQPDCKRPRRSLKKILQEASLPPWKRNTLPLLFCGDQLVWVAGIGIDCNFQAPMGR
AGLVVTWRPDQTNQSSIH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=416, Percent_Identity=35.3365384615385, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47536; Mature: 47536

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLHLLIKFSKLMPLDVSAIHVEHGISTYSGQWSAFCQTLCDSVAIPLSIHRLKISKQP
CHHHHHHHHHHHCCCCCHHEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
QESLEAVAREARYQIFRQIQADYVLLAQHQDDQVETLLLQLLRGAGVKGLSAMPTVRLFE
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
SGKAIKLLRPLLNVPRSEIMKYAKLHGLSWVTDESNLDVSYDRNFLRHRILPLIEQRYPS
CCCHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCH
YRKILSRSARHLGEAAHLLDELAKIDAENTMIVDKLSLRGLRELDLARAGNLLRYLLAQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHH
MVRLPSSAKLEEILRQLHNIQADTHFQFEVDALEIRCYRGLIEFLPADSLSESISPVIWQ
HHHCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHCCHHHHC
GQQRLAIEPLKGILEFTRQDNAGIDLAKLDQQIVTIRSRSGGERFQPDCKRPRRSLKKIL
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QEASLPPWKRNTLPLLFCGDQLVWVAGIGIDCNFQAPMGRAGLVVTWRPDQTNQSSIH
HHCCCCCCCCCCCEEEEECCCEEEEEECCEECCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MVLLHLLIKFSKLMPLDVSAIHVEHGISTYSGQWSAFCQTLCDSVAIPLSIHRLKISKQP
CHHHHHHHHHHHCCCCCHHEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
QESLEAVAREARYQIFRQIQADYVLLAQHQDDQVETLLLQLLRGAGVKGLSAMPTVRLFE
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
SGKAIKLLRPLLNVPRSEIMKYAKLHGLSWVTDESNLDVSYDRNFLRHRILPLIEQRYPS
CCCHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCH
YRKILSRSARHLGEAAHLLDELAKIDAENTMIVDKLSLRGLRELDLARAGNLLRYLLAQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHH
MVRLPSSAKLEEILRQLHNIQADTHFQFEVDALEIRCYRGLIEFLPADSLSESISPVIWQ
HHHCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHCCHHHHC
GQQRLAIEPLKGILEFTRQDNAGIDLAKLDQQIVTIRSRSGGERFQPDCKRPRRSLKKIL
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QEASLPPWKRNTLPLLFCGDQLVWVAGIGIDCNFQAPMGRAGLVVTWRPDQTNQSSIH
HHCCCCCCCCCCCEEEEECCCEEEEEECCEECCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]