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The map label for this gene is pcnB [H]

Identifier: 114332256

GI number: 114332256

Start: 2421166

End: 2422554

Strand: Reverse

Name: pcnB [H]

Synonym: Neut_2296

Alternate gene names: 114332256

Gene position: 2422554-2421166 (Counterclockwise)

Preceding gene: 114332257

Following gene: 114332255

Centisome position: 91.04

GC content: 49.6

Gene sequence:

>1389_bases
ATGATCCGTAAATTTCTGCATCATATTTTTTCCTTCGGCTCTACAGTTTCTGTTACAGCTGCCCCGGCATTCCGGGCCAT
TGCATGTGAGCGGCATGGAATTTCGCGCAGCAGCATTAGTGCCGGTTCGCTGAAAGTTGCACTGACTTTACAGCAAGCAG
GTTATTCTGCCTATATTGTGGGCGGTGCCGTCCGTGATCTGCTGTTAGGGCTGAAGCCCAAGGATTATGATATTGTCACT
GATGCCACGCCCGAAGAAGTGCGCGCAATCTTCCGTCATTCACGCATTATTGGTCGGCGTTTCCGGCTGGTGCACGTCAT
GAGCAAGGGAGAAACAGTGGAAGTATCCACTTTTCGCGGTAAGGCGGCTGATGATCTGATTCAGTCTGACAAAGAGTGTG
CCTATATCGATGCCACCGGACGGTTGTTGCACGATAACTTTTTTGGCAGCCAGGAAGAGGATGTAAGACGTCGTGATTTC
ACTATCAATGCCTTGTTCTATAATCCTGCTACAGAAGAGATCATTGATTATCTAAGTGGTTTCGAAGATGTCATGGCAAA
GCGGTTGAACATCATCGGAGATCCTGAGCAGCGTTACCGGGAAGATCCGGTACGGATGTTGCGGGCAGTGCGTCTTGCTG
CCAAACTGGGTATTCAGATCGATGACAGGACAGCTGCACCTATTGGTGATCTTGCGCCATTGTTACTGAATGTTCCAGCG
GCACGCCTGTTTGACGAGATGCTGAAGCTATTGTTTTCTGGTCATGCACTGACAGCTGTGATTGATCTGCGAACGCGAGG
ATTGCATCATGGGTTACTGCCGATGCTGGATGTAATTCTGGAGCAGCCTCTGGGTGAACGATTTATTTCTCTGGCACTAA
AAAATACAGATGAGCGGGTGCGGCAGGGTAAACCGGTTTCTCCCGGATTCTTATTTGCAGCGTTATTGTGGCATGAAGTG
TTGGCGGTATGGAATATTCATCTGAAGGCGGGTGAAAAAAATATTCCCGCATTATTACATAGAGCGATGGGCGATGTTCT
GGCGGCTCAGCGCCGCCGATTGGCCATTCCGCGCAGGCATGATGGCGTTATTTATGATATCTGGTCCTTGCAGCCAAGGT
TTCTGGCGCGCGCAGGTCGTAAGCCATTTCGCCTGCTGGAGCATCCACATTTCCCTGCCGCCTATGATTTTATGCTGTTG
CGGTGTAAAAGCGGAGAAATCGACGAAGAGCTGGGGCAATGGTGGCAAACATTCAAAAATACCGATAATGCTGCCCGGGA
AGCAATGTTGCTGCAGGAAGCCCCGACAAAACGTCGCAGAAAACGTCGCCGTAGCAGTCAATCGGAAAGTGTAAAAGTAG
ACTCTCCCATTAATCAGGATATTTCATGA

Upstream 100 bases:

>100_bases
CAATGCCCGATTGCCATTTTCTTTTGTGGGCCAGTGTTTCATCAAAGTCTAATTTGTTACCAGGTGTATAATTTGTCGTT
CACAGGTATTGCATCCAGGC

Downstream 100 bases:

>100_bases
CATCTGATTTTCATCCTGAATCTATCAGCCGTATTTTTATTGCATTAGGCAGCAACCTGGAAAATCCTTTGTCTCAGGTC
AGGCGTGGAATACTGCTGTT

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MIRKFLHHIFSFGSTVSVTAAPAFRAIACERHGISRSSISAGSLKVALTLQQAGYSAYIVGGAVRDLLLGLKPKDYDIVT
DATPEEVRAIFRHSRIIGRRFRLVHVMSKGETVEVSTFRGKAADDLIQSDKECAYIDATGRLLHDNFFGSQEEDVRRRDF
TINALFYNPATEEIIDYLSGFEDVMAKRLNIIGDPEQRYREDPVRMLRAVRLAAKLGIQIDDRTAAPIGDLAPLLLNVPA
ARLFDEMLKLLFSGHALTAVIDLRTRGLHHGLLPMLDVILEQPLGERFISLALKNTDERVRQGKPVSPGFLFAALLWHEV
LAVWNIHLKAGEKNIPALLHRAMGDVLAAQRRRLAIPRRHDGVIYDIWSLQPRFLARAGRKPFRLLEHPHFPAAYDFMLL
RCKSGEIDEELGQWWQTFKNTDNAAREAMLLQEAPTKRRRKRRRSSQSESVKVDSPINQDIS

Sequences:

>Translated_462_residues
MIRKFLHHIFSFGSTVSVTAAPAFRAIACERHGISRSSISAGSLKVALTLQQAGYSAYIVGGAVRDLLLGLKPKDYDIVT
DATPEEVRAIFRHSRIIGRRFRLVHVMSKGETVEVSTFRGKAADDLIQSDKECAYIDATGRLLHDNFFGSQEEDVRRRDF
TINALFYNPATEEIIDYLSGFEDVMAKRLNIIGDPEQRYREDPVRMLRAVRLAAKLGIQIDDRTAAPIGDLAPLLLNVPA
ARLFDEMLKLLFSGHALTAVIDLRTRGLHHGLLPMLDVILEQPLGERFISLALKNTDERVRQGKPVSPGFLFAALLWHEV
LAVWNIHLKAGEKNIPALLHRAMGDVLAAQRRRLAIPRRHDGVIYDIWSLQPRFLARAGRKPFRLLEHPHFPAAYDFMLL
RCKSGEIDEELGQWWQTFKNTDNAAREAMLLQEAPTKRRRKRRRSSQSESVKVDSPINQDIS
>Mature_462_residues
MIRKFLHHIFSFGSTVSVTAAPAFRAIACERHGISRSSISAGSLKVALTLQQAGYSAYIVGGAVRDLLLGLKPKDYDIVT
DATPEEVRAIFRHSRIIGRRFRLVHVMSKGETVEVSTFRGKAADDLIQSDKECAYIDATGRLLHDNFFGSQEEDVRRRDF
TINALFYNPATEEIIDYLSGFEDVMAKRLNIIGDPEQRYREDPVRMLRAVRLAAKLGIQIDDRTAAPIGDLAPLLLNVPA
ARLFDEMLKLLFSGHALTAVIDLRTRGLHHGLLPMLDVILEQPLGERFISLALKNTDERVRQGKPVSPGFLFAALLWHEV
LAVWNIHLKAGEKNIPALLHRAMGDVLAAQRRRLAIPRRHDGVIYDIWSLQPRFLARAGRKPFRLLEHPHFPAAYDFMLL
RCKSGEIDEELGQWWQTFKNTDNAAREAMLLQEAPTKRRRKRRRSSQSESVKVDSPINQDIS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=30.5825242718447, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI87081691, Length=418, Percent_Identity=45.4545454545455, Blast_Score=338, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI71995920, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=195, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281360112, Length=253, Percent_Identity=27.6679841897233, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357337, Length=253, Percent_Identity=27.6679841897233, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52251; Mature: 52251

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKFLHHIFSFGSTVSVTAAPAFRAIACERHGISRSSISAGSLKVALTLQQAGYSAYIV
CHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEE
GGAVRDLLLGLKPKDYDIVTDATPEEVRAIFRHSRIIGRRFRLVHVMSKGETVEVSTFRG
HHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCC
KAADDLIQSDKECAYIDATGRLLHDNFFGSQEEDVRRRDFTINALFYNPATEEIIDYLSG
CHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHCEEEEEEEECCCHHHHHHHHHC
FEDVMAKRLNIIGDPEQRYREDPVRMLRAVRLAAKLGIQIDDRTAAPIGDLAPLLLNVPA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHCCCH
ARLFDEMLKLLFSGHALTAVIDLRTRGLHHGLLPMLDVILEQPLGERFISLALKNTDERV
HHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
RQGKPVSPGFLFAALLWHEVLAVWNIHLKAGEKNIPALLHRAMGDVLAAQRRRLAIPRRH
HCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
DGVIYDIWSLQPRFLARAGRKPFRLLEHPHFPAAYDFMLLRCKSGEIDEELGQWWQTFKN
CCEEEECCCCCHHHHHHCCCCCHHHHCCCCCCCHHHHHEEEECCCCCHHHHHHHHHHHHC
TDNAAREAMLLQEAPTKRRRKRRRSSQSESVKVDSPINQDIS
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MIRKFLHHIFSFGSTVSVTAAPAFRAIACERHGISRSSISAGSLKVALTLQQAGYSAYIV
CHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEE
GGAVRDLLLGLKPKDYDIVTDATPEEVRAIFRHSRIIGRRFRLVHVMSKGETVEVSTFRG
HHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCC
KAADDLIQSDKECAYIDATGRLLHDNFFGSQEEDVRRRDFTINALFYNPATEEIIDYLSG
CHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHCEEEEEEEECCCHHHHHHHHHC
FEDVMAKRLNIIGDPEQRYREDPVRMLRAVRLAAKLGIQIDDRTAAPIGDLAPLLLNVPA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHCCCH
ARLFDEMLKLLFSGHALTAVIDLRTRGLHHGLLPMLDVILEQPLGERFISLALKNTDERV
HHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
RQGKPVSPGFLFAALLWHEVLAVWNIHLKAGEKNIPALLHRAMGDVLAAQRRRLAIPRRH
HCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
DGVIYDIWSLQPRFLARAGRKPFRLLEHPHFPAAYDFMLLRCKSGEIDEELGQWWQTFKN
CCEEEECCCCCHHHHHHCCCCCHHHHCCCCCCCHHHHHEEEECCCCCHHHHHHHHHHHHC
TDNAAREAMLLQEAPTKRRRKRRRSSQSESVKVDSPINQDIS
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]