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The map label for this gene is oprM [H]

Identifier: 114332123

GI number: 114332123

Start: 2285378

End: 2286772

Strand: Direct

Name: oprM [H]

Synonym: Neut_2158

Alternate gene names: 114332123

Gene position: 2285378-2286772 (Clockwise)

Preceding gene: 114332122

Following gene: 114332124

Centisome position: 85.88

GC content: 49.53

Gene sequence:

>1395_bases
TTGTTAATAACATCTTGCGCCATGGGCCCTGACTATTCACGCCCTCAGATCGATACCACAGAAAAATTTCGTCTGTCTCA
AACAGAGGGGCGGTCCATTGCCAATCTTCCTTGGTGGGAGCTGCTCCGGGATGAAAAGCTGCAATTGCTGATTGATCAAG
CCTTGACCGAAAACAAGGATCTCAAGCAAGCAGCAGCTAGTGTTGAAGAACTTCAGGCGCGCTTGGGTATTACACAGATG
GACTTCATTCCGAACATTACCATCGATGGGAATGCGCCGATAGAAACCTTAGGAGGATTCAGCCGCAAAGGATTTCCGAC
ACCCTATAACTATTTCGGACAAACCATTTTGAACTGGGAATTGGATATCTGGGGCAGAATCCGCCGCTCCAATGAAGCCG
CTCGGGCAGATCTGCTGGCACGCGAAGAAAATCGTCGAGCCGTTGTTCTGACGTTAGTAAGCTCGGTAGCACAATCCTAT
TTCGATCTTTTGCAATTTGATACGCAACTAAAAATTGCCCATCACGCATTAACTTCATGGGAAGAATCTGTCGCCATCTC
CAGTGCGCAACTGGAAGGTGGATTGACTACCCGGCTTGATCTGGATCAGTTTGAAGCAGAGCGTGCTGCAGCTGCTGCCC
AGGTGGCAGAGCTGGAAAGACAGATTGCACAAAAAGAAAATGAGCTTAGCGTGCTGGCGGGCAGAACCCCCGCGCCGGTA
GCACGAGGATACTCACTGAGCGGACAATCCACCCCTCCCGATATTCCACCCGGACTGCCTTCTGAATTACTGCAACGGCG
CCCTGATATTTTACAGGCAGAACAAACGCTGAAAGCAGCAATTGCTAGAATCGGAGTCGCCAAGGCCACGCGCTTTCCCA
GAATAACGCTGACAGGATTTTTTGGATTTTCCAGCCCGGCACTGGCAAGTCTGCTGGATTCTGACAGTAAATTTGGCGCT
GCTGGATTCGGGCTTGCGGGACCACTGCTAAATGCACAAACACTCGGATACGAACAACGTGCTGCAGAAGCACAGGCAAA
ACAGGCATTGGCACAGTACGAACAAACCATCCTGGTAGCGTTCAAGGAAGTTGAAGATGCACTAGCAGCCATTCGAACAG
CAAATGATCAGTACAAAGCCAAACAGAAACAGGTTGAAGCATTAAGTTCGGCTTTACAGATAGCAAATTTGCGCTATCAG
GGCGGAATTACAAGCTACGTTGATGTGTTGCTTACAAAGCGAAAATTATTTGATGCCGAATCTGCACTTGTGATCGCTCG
CCATTTACATCTAGTTTCTGTCGTCCAACTTTACAAAGCACTGGGTGGAGGCTGGCTCGCTGATGGTACCGATGTACCAC
TGCCAATATCAACCGGGCTGCTGCGCAACAACTGA

Upstream 100 bases:

>100_bases
ACTACCTCGGCTAACTGCAATATCCTATTTTAAAAATAGAAGGGCTATTTCAACCAGGCCAAATCGTCGTCAAACACCTG
TTCTGCTGTTATGTTCGAGC

Downstream 100 bases:

>100_bases
AATAAACTATAAATAGTACTTTCATGACTTTTATCCCAACTTAAGAATATCTTTTGATTATTTCTTCAGAGCTATCGAGA
ATAACATTATGTATCAAACG

Product: RND efflux system, outer membrane lipoprotein, NodT family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLITSCAMGPDYSRPQIDTTEKFRLSQTEGRSIANLPWWELLRDEKLQLLIDQALTENKDLKQAAASVEELQARLGITQM
DFIPNITIDGNAPIETLGGFSRKGFPTPYNYFGQTILNWELDIWGRIRRSNEAARADLLAREENRRAVVLTLVSSVAQSY
FDLLQFDTQLKIAHHALTSWEESVAISSAQLEGGLTTRLDLDQFEAERAAAAAQVAELERQIAQKENELSVLAGRTPAPV
ARGYSLSGQSTPPDIPPGLPSELLQRRPDILQAEQTLKAAIARIGVAKATRFPRITLTGFFGFSSPALASLLDSDSKFGA
AGFGLAGPLLNAQTLGYEQRAAEAQAKQALAQYEQTILVAFKEVEDALAAIRTANDQYKAKQKQVEALSSALQIANLRYQ
GGITSYVDVLLTKRKLFDAESALVIARHLHLVSVVQLYKALGGGWLADGTDVPLPISTGLLRNN

Sequences:

>Translated_464_residues
MLITSCAMGPDYSRPQIDTTEKFRLSQTEGRSIANLPWWELLRDEKLQLLIDQALTENKDLKQAAASVEELQARLGITQM
DFIPNITIDGNAPIETLGGFSRKGFPTPYNYFGQTILNWELDIWGRIRRSNEAARADLLAREENRRAVVLTLVSSVAQSY
FDLLQFDTQLKIAHHALTSWEESVAISSAQLEGGLTTRLDLDQFEAERAAAAAQVAELERQIAQKENELSVLAGRTPAPV
ARGYSLSGQSTPPDIPPGLPSELLQRRPDILQAEQTLKAAIARIGVAKATRFPRITLTGFFGFSSPALASLLDSDSKFGA
AGFGLAGPLLNAQTLGYEQRAAEAQAKQALAQYEQTILVAFKEVEDALAAIRTANDQYKAKQKQVEALSSALQIANLRYQ
GGITSYVDVLLTKRKLFDAESALVIARHLHLVSVVQLYKALGGGWLADGTDVPLPISTGLLRNN
>Mature_464_residues
MLITSCAMGPDYSRPQIDTTEKFRLSQTEGRSIANLPWWELLRDEKLQLLIDQALTENKDLKQAAASVEELQARLGITQM
DFIPNITIDGNAPIETLGGFSRKGFPTPYNYFGQTILNWELDIWGRIRRSNEAARADLLAREENRRAVVLTLVSSVAQSY
FDLLQFDTQLKIAHHALTSWEESVAISSAQLEGGLTTRLDLDQFEAERAAAAAQVAELERQIAQKENELSVLAGRTPAPV
ARGYSLSGQSTPPDIPPGLPSELLQRRPDILQAEQTLKAAIARIGVAKATRFPRITLTGFFGFSSPALASLLDSDSKFGA
AGFGLAGPLLNAQTLGYEQRAAEAQAKQALAQYEQTILVAFKEVEDALAAIRTANDQYKAKQKQVEALSSALQIANLRYQ
GGITSYVDVLLTKRKLFDAESALVIARHLHLVSVVQLYKALGGGWLADGTDVPLPISTGLLRNN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=449, Percent_Identity=33.630289532294, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI2367348, Length=474, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50759; Mature: 50759

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITSCAMGPDYSRPQIDTTEKFRLSQTEGRSIANLPWWELLRDEKLQLLIDQALTENKD
CEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
LKQAAASVEELQARLGITQMDFIPNITIDGNAPIETLGGFSRKGFPTPYNYFGQTILNWE
HHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCHHHHCCCCCCCCCCCHHHHCCCCEEEE
LDIWGRIRRSNEAARADLLAREENRRAVVLTLVSSVAQSYFDLLQFDTQLKIAHHALTSW
HHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EESVAISSAQLEGGLTTRLDLDQFEAERAAAAAQVAELERQIAQKENELSVLAGRTPAPV
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
ARGYSLSGQSTPPDIPPGLPSELLQRRPDILQAEQTLKAAIARIGVAKATRFPRITLTGF
HCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
FGFSSPALASLLDSDSKFGAAGFGLAGPLLNAQTLGYEQRAAEAQAKQALAQYEQTILVA
CCCCCHHHHHHHCCCCCCCCCCCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FKEVEDALAAIRTANDQYKAKQKQVEALSSALQIANLRYQGGITSYVDVLLTKRKLFDAE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
SALVIARHLHLVSVVQLYKALGGGWLADGTDVPLPISTGLLRNN
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLITSCAMGPDYSRPQIDTTEKFRLSQTEGRSIANLPWWELLRDEKLQLLIDQALTENKD
CEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
LKQAAASVEELQARLGITQMDFIPNITIDGNAPIETLGGFSRKGFPTPYNYFGQTILNWE
HHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCHHHHCCCCCCCCCCCHHHHCCCCEEEE
LDIWGRIRRSNEAARADLLAREENRRAVVLTLVSSVAQSYFDLLQFDTQLKIAHHALTSW
HHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EESVAISSAQLEGGLTTRLDLDQFEAERAAAAAQVAELERQIAQKENELSVLAGRTPAPV
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
ARGYSLSGQSTPPDIPPGLPSELLQRRPDILQAEQTLKAAIARIGVAKATRFPRITLTGF
HCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
FGFSSPALASLLDSDSKFGAAGFGLAGPLLNAQTLGYEQRAAEAQAKQALAQYEQTILVA
CCCCCHHHHHHHCCCCCCCCCCCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FKEVEDALAAIRTANDQYKAKQKQVEALSSALQIANLRYQGGITSYVDVLLTKRKLFDAE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
SALVIARHLHLVSVVQLYKALGGGWLADGTDVPLPISTGLLRNN
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]