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The map label for this gene is mexA [H]

Identifier: 114332124

GI number: 114332124

Start: 2286861

End: 2288066

Strand: Direct

Name: mexA [H]

Synonym: Neut_2159

Alternate gene names: 114332124

Gene position: 2286861-2288066 (Clockwise)

Preceding gene: 114332123

Following gene: 114332125

Centisome position: 85.94

GC content: 48.26

Gene sequence:

>1206_bases
ATGTATCAAACGCTTCGTCAAACAACCACGCAAGCCAATGCGGTTATAACAAGTTTCTTGTTGCTCCTTGCTCTTCTGGC
CGGTTGCTCAAAATCATCTACTGATTCTCCTCCTCCTGCTCCGCAGATTGAAGTCATCACTGTCACCACTCGTACAATTG
CGGAAGAACCTGAATTCATTGGCCAAACCGAATCTTTCCGGCCGGTTGAAATTCGTTCACAGGTAAATGGCATTATCAAA
AAGATATTCTTTATCGAAGGCCGAAATATCAGAAAAGGTGACAAGCTGTACTTGATTGATCCAACACCCTTCAAAGCAGC
CTATCTGGGTAGCAAAGCAATGGTTGCCCAGGCACGTGCCCGATTGGAGCAAGCCAACAAGGATCTTGCCCGGGTGCAAC
CCCTGCTGAAACAAAAAGCAGTCAGCCAAAAAGATGTTGACGATGCTATCGCAGAAGTACGTAGTGCAAAAGCAACTCTG
GATACGGCACAGAACGACGTAATCAAGGCCAAATTTGACCTGGATAACACGCTCATCACCGCTCCGGTAGAAGGCCGGAT
CAACCGCAGTCAATTTTACGAGGGGCGTCTGATCGAAGCCCAAACCGATCTACTGACCACCATTGATCAGCTAGACCCCA
TGTATGTCAACGTAAATGTTCCGGAAAGTTATTTGCTGCGCCTTCGCCGCGAACTTTCCGAGCACAAACTTGAAAGACCT
GAAAGTATTTTCCAGTTGCGCGGTGTAATGACTTTCTCTGATGGCAGTATTTATCCGGAAGAAGGGCTACTGGATTTTCA
AGATATTGTAATACGACCCGAAACTGGCATGCTGCTAGGACGCTTCGCGTTTCCCAACCCTGCTAGTAAGAATGCTCCCG
GAGAAGCTCATTTCTATCCAGGCCAGTTTGTTAAAGTTCGTATTAAAGGTTACAGCAGAGCAGATGCCATTCTGATTCCT
CAGCGTGCCGTGCAACAACGACCAAGCGGCTCGTTCGTTTATATCATCAATGAAGGAAAGGTTGAACTCCGCCCGGTACT
GGCCAGCGCATGGCGCGGCAGTGAATGGCTGATTGAAAGCGGCCTGCGCACGGGAGAACAAGTGGTTGTAGAAGGGATTC
ACAGAATCCATCCGGGAATCCAAGTAAACCCTGTTCCCTACCAGAGAGAAGAAAGCCAGACTTCCTCAAGCAACCATCCA
GAATAA

Upstream 100 bases:

>100_bases
CGCAACAACTGAAATAAACTATAAATAGTACTTTCATGACTTTTATCCCAACTTAAGAATATCTTTTGATTATTTCTTCA
GAGCTATCGAGAATAACATT

Downstream 100 bases:

>100_bases
ACCGCGTAAGGCGTGCCATGAATTCTCGTTTTTTTATTGAACGACCGATTTTTGCATCGGTACTATCCATCATCATTGTT
ATTGTCGGCCTGGTTTCCTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MYQTLRQTTTQANAVITSFLLLLALLAGCSKSSTDSPPPAPQIEVITVTTRTIAEEPEFIGQTESFRPVEIRSQVNGIIK
KIFFIEGRNIRKGDKLYLIDPTPFKAAYLGSKAMVAQARARLEQANKDLARVQPLLKQKAVSQKDVDDAIAEVRSAKATL
DTAQNDVIKAKFDLDNTLITAPVEGRINRSQFYEGRLIEAQTDLLTTIDQLDPMYVNVNVPESYLLRLRRELSEHKLERP
ESIFQLRGVMTFSDGSIYPEEGLLDFQDIVIRPETGMLLGRFAFPNPASKNAPGEAHFYPGQFVKVRIKGYSRADAILIP
QRAVQQRPSGSFVYIINEGKVELRPVLASAWRGSEWLIESGLRTGEQVVVEGIHRIHPGIQVNPVPYQREESQTSSSNHP
E

Sequences:

>Translated_401_residues
MYQTLRQTTTQANAVITSFLLLLALLAGCSKSSTDSPPPAPQIEVITVTTRTIAEEPEFIGQTESFRPVEIRSQVNGIIK
KIFFIEGRNIRKGDKLYLIDPTPFKAAYLGSKAMVAQARARLEQANKDLARVQPLLKQKAVSQKDVDDAIAEVRSAKATL
DTAQNDVIKAKFDLDNTLITAPVEGRINRSQFYEGRLIEAQTDLLTTIDQLDPMYVNVNVPESYLLRLRRELSEHKLERP
ESIFQLRGVMTFSDGSIYPEEGLLDFQDIVIRPETGMLLGRFAFPNPASKNAPGEAHFYPGQFVKVRIKGYSRADAILIP
QRAVQQRPSGSFVYIINEGKVELRPVLASAWRGSEWLIESGLRTGEQVVVEGIHRIHPGIQVNPVPYQREESQTSSSNHP
E
>Mature_401_residues
MYQTLRQTTTQANAVITSFLLLLALLAGCSKSSTDSPPPAPQIEVITVTTRTIAEEPEFIGQTESFRPVEIRSQVNGIIK
KIFFIEGRNIRKGDKLYLIDPTPFKAAYLGSKAMVAQARARLEQANKDLARVQPLLKQKAVSQKDVDDAIAEVRSAKATL
DTAQNDVIKAKFDLDNTLITAPVEGRINRSQFYEGRLIEAQTDLLTTIDQLDPMYVNVNVPESYLLRLRRELSEHKLERP
ESIFQLRGVMTFSDGSIYPEEGLLDFQDIVIRPETGMLLGRFAFPNPASKNAPGEAHFYPGQFVKVRIKGYSRADAILIP
QRAVQQRPSGSFVYIINEGKVELRPVLASAWRGSEWLIESGLRTGEQVVVEGIHRIHPGIQVNPVPYQREESQTSSSNHP
E

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=37.2972972972973, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1786668, Length=381, Percent_Identity=34.1207349081365, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=30.8900523560209, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082036, Length=368, Percent_Identity=29.0760869565217, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87081796, Length=356, Percent_Identity=24.438202247191, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081951, Length=119, Percent_Identity=34.453781512605, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44842; Mature: 44842

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQTLRQTTTQANAVITSFLLLLALLAGCSKSSTDSPPPAPQIEVITVTTRTIAEEPEFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHCCHHHC
GQTESFRPVEIRSQVNGIIKKIFFIEGRNIRKGDKLYLIDPTPFKAAYLGSKAMVAQARA
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHCCCHHHHHHHHH
RLEQANKDLARVQPLLKQKAVSQKDVDDAIAEVRSAKATLDTAQNDVIKAKFDLDNTLIT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
APVEGRINRSQFYEGRLIEAQTDLLTTIDQLDPMYVNVNVPESYLLRLRRELSEHKLERP
ECCCCCCCHHHHCCCEEEEHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCH
ESIFQLRGVMTFSDGSIYPEEGLLDFQDIVIRPETGMLLGRFAFPNPASKNAPGEAHFYP
HHHHHHHCEEEECCCCCCCCCCCCCHHHEEECCCCCCEEEEECCCCCCCCCCCCCCEECC
GQFVKVRIKGYSRADAILIPQRAVQQRPSGSFVYIINEGKVELRPVLASAWRGSEWLIES
CCEEEEEEECCCCCCEEEECHHHHHCCCCCCEEEEEECCCEEEEHHHHHHCCCCHHHHHH
GLRTGEQVVVEGIHRIHPGIQVNPVPYQREESQTSSSNHPE
CCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYQTLRQTTTQANAVITSFLLLLALLAGCSKSSTDSPPPAPQIEVITVTTRTIAEEPEFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHCCHHHC
GQTESFRPVEIRSQVNGIIKKIFFIEGRNIRKGDKLYLIDPTPFKAAYLGSKAMVAQARA
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHCCCHHHHHHHHH
RLEQANKDLARVQPLLKQKAVSQKDVDDAIAEVRSAKATLDTAQNDVIKAKFDLDNTLIT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
APVEGRINRSQFYEGRLIEAQTDLLTTIDQLDPMYVNVNVPESYLLRLRRELSEHKLERP
ECCCCCCCHHHHCCCEEEEHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCH
ESIFQLRGVMTFSDGSIYPEEGLLDFQDIVIRPETGMLLGRFAFPNPASKNAPGEAHFYP
HHHHHHHCEEEECCCCCCCCCCCCCHHHEEECCCCCCEEEEECCCCCCCCCCCCCCEECC
GQFVKVRIKGYSRADAILIPQRAVQQRPSGSFVYIINEGKVELRPVLASAWRGSEWLIES
CCEEEEEEECCCCCCEEEECHHHHHCCCCCCEEEEEECCCEEEEHHHHHHCCCCHHHHHH
GLRTGEQVVVEGIHRIHPGIQVNPVPYQREESQTSSSNHPE
CCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]