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The map label for this gene is pnp [H]

Identifier: 114332122

GI number: 114332122

Start: 2282692

End: 2284809

Strand: Direct

Name: pnp [H]

Synonym: Neut_2157

Alternate gene names: 114332122

Gene position: 2282692-2284809 (Clockwise)

Preceding gene: 114332121

Following gene: 114332123

Centisome position: 85.78

GC content: 46.98

Gene sequence:

>2118_bases
TTGAAACCGATAAAAAAAAGCATAACTTACGGACACCATACGCTCACCATTGAAACTGGAGAAATTGCGAAACAGGCACA
TGGTGCTGTCATGGTTTCCATGGATGACACGGTTGTGCTGGTTACAGTAGTAGGAGATAAAAAAACCAAACCTGGACAGG
ATTTTTTCCCGCTCACAGTTGATTATCAGGAAAAATTTTATTCTGCCGGGCGCATTCCAGGAAGCTTCTTTAAACGTGAA
GGTCGTCCTTCTGAAAAGGAAATACTAACATCCCGGCTGATTGATCGGCCGATACGTCCGCTCTTCCCCGATGGTTTTTA
CAATGAGGTACAAGTCATCGCAATGGTATTGTCATCAGATGCTGAAATCGATGCTGACATTCCTGCAACGATCGGCGCAT
CTGCGGCACTGATACTCTCCGGTATCCCATTTGATGGGCCTATCGGTGCAGCACGCGTAGGTTATATTGACAACGAATAT
GTCCTTAATCCGACCACTACCCAGCTCAATAAATCGCAACTCAATCTCGTAGTGGCAGGCACCCAAAAAGCTGTGCTGAT
GGTTGAGTCAGAAGCTAATGAACTCTCCGAAGATGTCATGCTCGGAGCAGTAACCTATGGCCATGATCAAATGCAGCAGG
TAATCAACATGATCAATGAACTGGCTGATGAGACTGGTGTAACCGCATGGGATTGGCAACCGGCAGAAAAAGATCATTCA
CTGATTGAAAAAATTACTCAACTGGCTGAAGCGGATTTGCGCGATGCATTCCGGCTGAAACAAAAATCGGTCAGAACAGA
AGCAATTAGTGAAATCTGGCAACGTGTATTTACCGAATTGAAAGCAGGTACTGAGGAAGGCCCCAGTGAGCAAACGATCA
AGGAAATCGGTTTTGCGCTTGAAGCCAAAATTGTGCGTAACAGCATCCTTGATGGTGAATCCCGCATTGACGGCCGCGGA
ACACGTACAGTTAGACCAATTACCATTCGCCATGGCGTATTACCACGTACTCATGGTTCAGCACTGTTCACACGAGGTGA
GACGCAGGCTTTGGTAGTGACCACACTGGGAACTGCCCGAGATGAGCAGAAAATTGATGCACTCCAAGGGGATTATTCCG
AACGTTTCATGCTGCATTACAACATGCCTCCTTTTGCCACGGGTGAAACCGGAAGAGTGGGATCACCCAAGCGGCGTGAA
ATTGGCCACGGACGATTGGCCAAGCGTGCTCTGCTTGCTGTCCTGCCGCCAGTGGAAGAATTCGGCTATGCGATGCGGGT
AGTCTCTGAAGTTACGGAATCAAATGGTTCAAGTTCGATGGCTTCTGTCTGCGGAGGTTGTCTTGCACTGATGGATGCGG
GAGCTCCCCTGAAAGCTCATGTCGCTGGTATTGCTATGGGGCTGATCAAGGAAGGCAATCGATTTGCCGTACTGACTGAT
ATCCTTGGTGATGAAGATCATCTGGGCGATATGGATTTTAAGGTCGCAGGGACTGAAGAGGGTATCACTGCATTACAAAT
GGATATAAAAATCCAGGGAATCACGAAAGAAATCATGCAGGTAGCCTTACTTCAAGCAAAAGAAGGGCGACTCCATATTC
TGGATATCATGAAACAATCTCTCCCAATAGCAAGAGATAGCGTATCGGTTCACGCACCACGTATTATCAAATTCAAAATT
AATCCTGAAAAAATACGTGATGTGATCGGTAAAGGCGGGGCAGTTATTCGCGCACTGACCGAGGAAACCGGAACCACTAT
CGATATCTCCGATGATGGTTCTGTGACAATTGCCAGCATCAGCAATGAAGGTGGGGAACAGGCGAAAAGACGTATCGAAA
ATATTACTGCAGATGTTGAGGTTGGCAAGATTTATGAGGGAACAGTACTCAAATTGCTTGATTTCGGTGCTATTGTCAGC
ATTCTTCCTGGAAAAGATGGTCTGCTCCATATCTCGCAAATTGCCAATGAACGAGTCGAAAGCGTTGCTGACCACCTCAA
GGAAGGCCAGATTATTCGGGTCAAAGTACTGGAAGCAGACGATAAAGGTCGGTTACGACTCAGCATGAAGGCAGCCAGCA
CAGAAGCAGCAGCCACTCCTGAAAACGGTGAAAAATAA

Upstream 100 bases:

>100_bases
AGTCGTCTGCACTCTAATTTGAATAACCCTGATCGATTTGCACTTAAATATAATTGTGCAGTCGATCTTGGGCATGATTT
GATCAAAGAAGGATTATTAA

Downstream 100 bases:

>100_bases
GCTGGTGTTATCCGTTTAATAAATCACAATCTGTCTCCACTTCGTAATGTTCTCCGGAGACCATGCTTATTTCAATCAAG
AAATAGCACCTCCGGAGAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKPIKKSITYGHHTLTIETGEIAKQAHGAVMVSMDDTVVLVTVVGDKKTKPGQDFFPLTVDYQEKFYSAGRIPGSFFKRE
GRPSEKEILTSRLIDRPIRPLFPDGFYNEVQVIAMVLSSDAEIDADIPATIGASAALILSGIPFDGPIGAARVGYIDNEY
VLNPTTTQLNKSQLNLVVAGTQKAVLMVESEANELSEDVMLGAVTYGHDQMQQVINMINELADETGVTAWDWQPAEKDHS
LIEKITQLAEADLRDAFRLKQKSVRTEAISEIWQRVFTELKAGTEEGPSEQTIKEIGFALEAKIVRNSILDGESRIDGRG
TRTVRPITIRHGVLPRTHGSALFTRGETQALVVTTLGTARDEQKIDALQGDYSERFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALLAVLPPVEEFGYAMRVVSEVTESNGSSSMASVCGGCLALMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTEEGITALQMDIKIQGITKEIMQVALLQAKEGRLHILDIMKQSLPIARDSVSVHAPRIIKFKI
NPEKIRDVIGKGGAVIRALTEETGTTIDISDDGSVTIASISNEGGEQAKRRIENITADVEVGKIYEGTVLKLLDFGAIVS
ILPGKDGLLHISQIANERVESVADHLKEGQIIRVKVLEADDKGRLRLSMKAASTEAAATPENGEK

Sequences:

>Translated_705_residues
MKPIKKSITYGHHTLTIETGEIAKQAHGAVMVSMDDTVVLVTVVGDKKTKPGQDFFPLTVDYQEKFYSAGRIPGSFFKRE
GRPSEKEILTSRLIDRPIRPLFPDGFYNEVQVIAMVLSSDAEIDADIPATIGASAALILSGIPFDGPIGAARVGYIDNEY
VLNPTTTQLNKSQLNLVVAGTQKAVLMVESEANELSEDVMLGAVTYGHDQMQQVINMINELADETGVTAWDWQPAEKDHS
LIEKITQLAEADLRDAFRLKQKSVRTEAISEIWQRVFTELKAGTEEGPSEQTIKEIGFALEAKIVRNSILDGESRIDGRG
TRTVRPITIRHGVLPRTHGSALFTRGETQALVVTTLGTARDEQKIDALQGDYSERFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALLAVLPPVEEFGYAMRVVSEVTESNGSSSMASVCGGCLALMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTEEGITALQMDIKIQGITKEIMQVALLQAKEGRLHILDIMKQSLPIARDSVSVHAPRIIKFKI
NPEKIRDVIGKGGAVIRALTEETGTTIDISDDGSVTIASISNEGGEQAKRRIENITADVEVGKIYEGTVLKLLDFGAIVS
ILPGKDGLLHISQIANERVESVADHLKEGQIIRVKVLEADDKGRLRLSMKAASTEAAATPENGEK
>Mature_705_residues
MKPIKKSITYGHHTLTIETGEIAKQAHGAVMVSMDDTVVLVTVVGDKKTKPGQDFFPLTVDYQEKFYSAGRIPGSFFKRE
GRPSEKEILTSRLIDRPIRPLFPDGFYNEVQVIAMVLSSDAEIDADIPATIGASAALILSGIPFDGPIGAARVGYIDNEY
VLNPTTTQLNKSQLNLVVAGTQKAVLMVESEANELSEDVMLGAVTYGHDQMQQVINMINELADETGVTAWDWQPAEKDHS
LIEKITQLAEADLRDAFRLKQKSVRTEAISEIWQRVFTELKAGTEEGPSEQTIKEIGFALEAKIVRNSILDGESRIDGRG
TRTVRPITIRHGVLPRTHGSALFTRGETQALVVTTLGTARDEQKIDALQGDYSERFMLHYNMPPFATGETGRVGSPKRRE
IGHGRLAKRALLAVLPPVEEFGYAMRVVSEVTESNGSSSMASVCGGCLALMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTEEGITALQMDIKIQGITKEIMQVALLQAKEGRLHILDIMKQSLPIARDSVSVHAPRIIKFKI
NPEKIRDVIGKGGAVIRALTEETGTTIDISDDGSVTIASISNEGGEQAKRRIENITADVEVGKIYEGTVLKLLDFGAIVS
ILPGKDGLLHISQIANERVESVADHLKEGQIIRVKVLEADDKGRLRLSMKAASTEAAATPENGEK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=39.6358543417367, Blast_Score=444, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=63.5057471264368, Blast_Score=893, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=731, Percent_Identity=33.515731874145, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17535281, Length=74, Percent_Identity=47.2972972972973, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281362905, Length=719, Percent_Identity=38.1084840055633, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=719, Percent_Identity=38.1084840055633, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=719, Percent_Identity=38.1084840055633, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=665, Percent_Identity=37.8947368421053, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76601; Mature: 76601

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIKKSITYGHHTLTIETGEIAKQAHGAVMVSMDDTVVLVTVVGDKKTKPGQDFFPLTV
CCCCHHHCCCCCEEEEEECHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCCCCCCEEE
DYQEKFYSAGRIPGSFFKREGRPSEKEILTSRLIDRPIRPLFPDGFYNEVQVIAMVLSSD
CHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
AEIDADIPATIGASAALILSGIPFDGPIGAARVGYIDNEYVLNPTTTQLNKSQLNLVVAG
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEC
TQKAVLMVESEANELSEDVMLGAVTYGHDQMQQVINMINELADETGVTAWDWQPAEKDHS
CCEEEEEEECCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHH
LIEKITQLAEADLRDAFRLKQKSVRTEAISEIWQRVFTELKAGTEEGPSEQTIKEIGFAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EAKIVRNSILDGESRIDGRGTRTVRPITIRHGVLPRTHGSALFTRGETQALVVTTLGTAR
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCC
DEQKIDALQGDYSERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALLAVLPPVEE
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHH
FGYAMRVVSEVTESNGSSSMASVCGGCLALMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
ILGDEDHLGDMDFKVAGTEEGITALQMDIKIQGITKEIMQVALLQAKEGRLHILDIMKQS
CCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHC
LPIARDSVSVHAPRIIKFKINPEKIRDVIGKGGAVIRALTEETGTTIDISDDGSVTIASI
CCCCCCCCEECCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEE
SNEGGEQAKRRIENITADVEVGKIYEGTVLKLLDFGAIVSILPGKDGLLHISQIANERVE
CCCCHHHHHHHHHHCCCCEEECCEECCHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
SVADHLKEGQIIRVKVLEADDKGRLRLSMKAASTEAAATPENGEK
HHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKPIKKSITYGHHTLTIETGEIAKQAHGAVMVSMDDTVVLVTVVGDKKTKPGQDFFPLTV
CCCCHHHCCCCCEEEEEECHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCCCCCCEEE
DYQEKFYSAGRIPGSFFKREGRPSEKEILTSRLIDRPIRPLFPDGFYNEVQVIAMVLSSD
CHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
AEIDADIPATIGASAALILSGIPFDGPIGAARVGYIDNEYVLNPTTTQLNKSQLNLVVAG
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEC
TQKAVLMVESEANELSEDVMLGAVTYGHDQMQQVINMINELADETGVTAWDWQPAEKDHS
CCEEEEEEECCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHH
LIEKITQLAEADLRDAFRLKQKSVRTEAISEIWQRVFTELKAGTEEGPSEQTIKEIGFAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EAKIVRNSILDGESRIDGRGTRTVRPITIRHGVLPRTHGSALFTRGETQALVVTTLGTAR
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCC
DEQKIDALQGDYSERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALLAVLPPVEE
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHH
FGYAMRVVSEVTESNGSSSMASVCGGCLALMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
ILGDEDHLGDMDFKVAGTEEGITALQMDIKIQGITKEIMQVALLQAKEGRLHILDIMKQS
CCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHC
LPIARDSVSVHAPRIIKFKINPEKIRDVIGKGGAVIRALTEETGTTIDISDDGSVTIASI
CCCCCCCCEECCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEE
SNEGGEQAKRRIENITADVEVGKIYEGTVLKLLDFGAIVSILPGKDGLLHISQIANERVE
CCCCHHHHHHHHHHCCCCEEECCEECCHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
SVADHLKEGQIIRVKVLEADDKGRLRLSMKAASTEAAATPENGEK
HHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]