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The map label for this gene is bioF

Identifier: 114332102

GI number: 114332102

Start: 2265934

End: 2267097

Strand: Reverse

Name: bioF

Synonym: Neut_2137

Alternate gene names: 114332102

Gene position: 2267097-2265934 (Counterclockwise)

Preceding gene: 114332103

Following gene: 114332101

Centisome position: 85.2

GC content: 56.44

Gene sequence:

>1164_bases
ATGCTGCCGGACTTGGCTGAAGCGCTGCAACAGCGCAGGCAGGAAGGGTTGTATCGTTTTCGCCAGGTGCTGGAAGGGCC
GCAATCCCCGCGTGTGACCATTGATGGCCGGGATTTTCTGGCTTTTTGCAGTAACGATTATCTGGGGCTGGCTAATCATC
CAGCCCTGATCGAAGCAGTAGCAGCAGGTGCACAACGTTACGGTGTGGGTTCGGGCGCTTCTCATCTGATTAGTGGTCAT
TCCCGCGCACATCATGAACTGGAAGAGGCACTGGCGGAGTTCGTCGGCCTTCCACGTACCCTGCTGTTTTCCACGGGCTA
CATGGCCAATATGGCGGTGGTGACTGCGCTCATGGGGCGGGAAGATGCGATTTTTGCAGATCGTCTCAATCATGCTTCAC
TCAATGACGCGGCTTTGCTGTCACGTGCCCGCTTCATTCGTTATCCCCATCTTGATCTTGTTACTCTGGAGAAGCAGCTG
AAGACGATACAAGCCCGGCGGCGGCTGATCGTCACCGACGCAGTATTCAGTATGGATGGTGACAGGGCGCCGGTTGCCGA
GCTGCTGGCGTTGTGTCAGCGCTTTGATGCCTGGTTGTTGCTGGATGATGCGCACGGATTTGGTGTGCTGGGTGAACAGG
GCAAAGGCAGTTTGTATGATCCGCAGGAGGTGGAGCGAAATGTACCACATCTGATTTACATGGCAACGCTGGGCAAGGCA
GCAGGAGTATCCGGCGCGTTCGTGGCAGCGCAAGCGTCAATGATTGAAACGCTCATTCAGCATAGCCGTACCTATGGCTA
TACCACGGCGGCAGCCCCGTTGCTGGCACATGCCTTGCTGACCAGTTTACAATTAATCAGTCAGGGTGTCTGGCGACGGG
AGCGGCTTGTTCAATTGATAGAGCAGTTGCGACAAAAGCTGCAATCTCTCCCCTGGCAACTATTGCTATCGGATACACCG
ATACAGCCATTGCTGGTGGGCGGAAGCCGGGAGGCCGTCCGGCTGGACCAGGCGTTGCGTGAGCGTGGTATCTGGGTGCC
GGCTATTCGCCCTCCGACAGTGCCGCAAGGCATGGCACGGTTGCGCATCAGTTTGTCTGCGGCTCATGCTGGGGAAGATG
TCGATCAATTGAGTGCAGCCTTGCATGATCTGGCTGGATGCTGA

Upstream 100 bases:

>100_bases
CTATCTTTTATGGTGACAAACTGCTCACGACCGGCAATCCGGATACGGAACAGGACGCAGCTTTGCTCGAAAAACTGGGA
TTGCATGCACTTTAACCACC

Downstream 100 bases:

>100_bases
TATGACCCGGTTGCATATTGAGACAAGCGGGAGCGGGCCTGATCTGGTGATGTTGCATGGCTGGGCCATGCACAGTGGTG
TATGGGAGTGTGTGTCAGAG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLPDLAEALQQRRQEGLYRFRQVLEGPQSPRVTIDGRDFLAFCSNDYLGLANHPALIEAVAAGAQRYGVGSGASHLISGH
SRAHHELEEALAEFVGLPRTLLFSTGYMANMAVVTALMGREDAIFADRLNHASLNDAALLSRARFIRYPHLDLVTLEKQL
KTIQARRRLIVTDAVFSMDGDRAPVAELLALCQRFDAWLLLDDAHGFGVLGEQGKGSLYDPQEVERNVPHLIYMATLGKA
AGVSGAFVAAQASMIETLIQHSRTYGYTTAAAPLLAHALLTSLQLISQGVWRRERLVQLIEQLRQKLQSLPWQLLLSDTP
IQPLLVGGSREAVRLDQALRERGIWVPAIRPPTVPQGMARLRISLSAAHAGEDVDQLSAALHDLAGC

Sequences:

>Translated_387_residues
MLPDLAEALQQRRQEGLYRFRQVLEGPQSPRVTIDGRDFLAFCSNDYLGLANHPALIEAVAAGAQRYGVGSGASHLISGH
SRAHHELEEALAEFVGLPRTLLFSTGYMANMAVVTALMGREDAIFADRLNHASLNDAALLSRARFIRYPHLDLVTLEKQL
KTIQARRRLIVTDAVFSMDGDRAPVAELLALCQRFDAWLLLDDAHGFGVLGEQGKGSLYDPQEVERNVPHLIYMATLGKA
AGVSGAFVAAQASMIETLIQHSRTYGYTTAAAPLLAHALLTSLQLISQGVWRRERLVQLIEQLRQKLQSLPWQLLLSDTP
IQPLLVGGSREAVRLDQALRERGIWVPAIRPPTVPQGMARLRISLSAAHAGEDVDQLSAALHDLAGC
>Mature_387_residues
MLPDLAEALQQRRQEGLYRFRQVLEGPQSPRVTIDGRDFLAFCSNDYLGLANHPALIEAVAAGAQRYGVGSGASHLISGH
SRAHHELEEALAEFVGLPRTLLFSTGYMANMAVVTALMGREDAIFADRLNHASLNDAALLSRARFIRYPHLDLVTLEKQL
KTIQARRRLIVTDAVFSMDGDRAPVAELLALCQRFDAWLLLDDAHGFGVLGEQGKGSLYDPQEVERNVPHLIYMATLGKA
AGVSGAFVAAQASMIETLIQHSRTYGYTTAAAPLLAHALLTSLQLISQGVWRRERLVQLIEQLRQKLQSLPWQLLLSDTP
IQPLLVGGSREAVRLDQALRERGIWVPAIRPPTVPQGMARLRISLSAAHAGEDVDQLSAALHDLAGC

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=378, Percent_Identity=32.8042328042328, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI284448556, Length=404, Percent_Identity=30.6930693069307, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI4758668, Length=354, Percent_Identity=30.5084745762712, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI119220554, Length=371, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI83977440, Length=380, Percent_Identity=28.4210526315789, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI83977442, Length=380, Percent_Identity=28.4210526315789, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI83977444, Length=380, Percent_Identity=28.4210526315789, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4502025, Length=344, Percent_Identity=29.0697674418605, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI40316939, Length=344, Percent_Identity=29.0697674418605, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI5454084, Length=206, Percent_Identity=28.1553398058252, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786993, Length=350, Percent_Identity=46.8571428571429, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790046, Length=375, Percent_Identity=37.3333333333333, Blast_Score=246, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71994529, Length=383, Percent_Identity=34.4647519582245, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17560914, Length=356, Percent_Identity=27.8089887640449, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560912, Length=356, Percent_Identity=27.8089887640449, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566772, Length=364, Percent_Identity=25.5494505494505, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71982617, Length=331, Percent_Identity=23.8670694864048, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=331, Percent_Identity=23.8670694864048, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320267, Length=357, Percent_Identity=29.6918767507003, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320438, Length=405, Percent_Identity=26.9135802469136, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323954, Length=299, Percent_Identity=26.7558528428094, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24662918, Length=368, Percent_Identity=34.5108695652174, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17137420, Length=357, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17136286, Length=372, Percent_Identity=26.8817204301075, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24653280, Length=309, Percent_Identity=26.8608414239482, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653276, Length=309, Percent_Identity=26.8608414239482, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653278, Length=309, Percent_Identity=26.8608414239482, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_NITEC (Q0AE73)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748324.1
- ProteinModelPortal:   Q0AE73
- STRING:   Q0AE73
- GeneID:   4274179
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2137
- NMPDR:   fig|335283.3.peg.172
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- PhylomeDB:   Q0AE73
- ProtClustDB:   CLSK586188
- BioCyc:   NEUT335283:NEUT_2137-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42405; Mature: 42405

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 19-19 BINDING 131-131 BINDING 177-177 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDLAEALQQRRQEGLYRFRQVLEGPQSPRVTIDGRDFLAFCSNDYLGLANHPALIEAV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHH
AAGAQRYGVGSGASHLISGHSRAHHELEEALAEFVGLPRTLLFSTGYMANMAVVTALMGR
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCC
EDAIFADRLNHASLNDAALLSRARFIRYPHLDLVTLEKQLKTIQARRRLIVTDAVFSMDG
CCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHHCCC
DRAPVAELLALCQRFDAWLLLDDAHGFGVLGEQGKGSLYDPQEVERNVPHLIYMATLGKA
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEHHCHH
AGVSGAFVAAQASMIETLIQHSRTYGYTTAAAPLLAHALLTSLQLISQGVWRRERLVQLI
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLRQKLQSLPWQLLLSDTPIQPLLVGGSREAVRLDQALRERGIWVPAIRPPTVPQGMAR
HHHHHHHHHCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LRISLSAAHAGEDVDQLSAALHDLAGC
HHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPDLAEALQQRRQEGLYRFRQVLEGPQSPRVTIDGRDFLAFCSNDYLGLANHPALIEAV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHH
AAGAQRYGVGSGASHLISGHSRAHHELEEALAEFVGLPRTLLFSTGYMANMAVVTALMGR
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCC
EDAIFADRLNHASLNDAALLSRARFIRYPHLDLVTLEKQLKTIQARRRLIVTDAVFSMDG
CCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHHCCC
DRAPVAELLALCQRFDAWLLLDDAHGFGVLGEQGKGSLYDPQEVERNVPHLIYMATLGKA
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEHHCHH
AGVSGAFVAAQASMIETLIQHSRTYGYTTAAAPLLAHALLTSLQLISQGVWRRERLVQLI
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLRQKLQSLPWQLLLSDTPIQPLLVGGSREAVRLDQALRERGIWVPAIRPPTVPQGMAR
HHHHHHHHHCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LRISLSAAHAGEDVDQLSAALHDLAGC
HHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA