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The map label for this gene is bioB

Identifier: 114332103

GI number: 114332103

Start: 2267103

End: 2268119

Strand: Reverse

Name: bioB

Synonym: Neut_2138

Alternate gene names: 114332103

Gene position: 2268119-2267103 (Counterclockwise)

Preceding gene: 114332108

Following gene: 114332102

Centisome position: 85.23

GC content: 51.23

Gene sequence:

>1017_bases
ATGGAATCCCGAACCGATTTAATTTCTGAAAAACAGTGCGGATGCACATATTCCAACCCTGATTTGGCAGTACAGCAAGA
ATCATCGTCACGCTGGTTGGTGGCAGATGTTGAATCACTGTTGGGTTTGCCGTTTAATGATCTGATTTTTCAGGCGCAAA
CGGTGCACCGCCAATATCACGATGCTAATGGCGTGCAATTGTCTACGCTGCTGTCAGTAAAAACTGGTGGATGTTCTGAA
GACTGCGCCTATTGCCCGCAGGCGGCTCGTTATCATACCGGGGTGGAAAATCAAGCAATTTTGTCGCGTGAGGAAGTAGT
GATGGCGGCCACCAAAGCCAAGGAAAGTGGCGCAACGCGCTTCTGCATGGGAGCCGCCTGGCGGGGACCCAAGCAACGTG
ATATTGAGCACATGACCGAGCTCATCAGTGCAGTTAAGGATTTAGGGCTGGAAACCTGTGCTACATTGGGTATCCTGAAA
CCCGGGCAGGCCGTTCAACTCAAGCAGGCCGGGTTGGATTACTACAATCACAATCTCGATACCTCTCCTGAGTTTTATGG
CGAGGTCATTACTACACGCGAATATCAGAATCGCCTGGATACATTGGACGAAGTGCGCGGTGCCAATATCAATGTTTGTT
GTGGTGGCATTGTTGGCATGGGGGAATCACGTACAGCCCGAGCCGGCCTGATTGCACAACTGGCCAGCCTTGATCCTTAT
CCTGAATCAGTGCCGATCAATTACCTGGTGCAGGTCGAAGGCACACCTTTGTATGGTATACCTGAGCTCGACCCGTTTGA
ATTTGTCCGCTCTATCGCAGCAGCCCGTATCACCATGCCCAGAGCAAAGGTCAGGCTTTCTGCTGGCCGCCGACAGATGT
CGGAAGCTATTCAGGCACTGTGTTTTCTGGCCGGTGCCAACTCTATCTTTTATGGTGACAAACTGCTCACGACCGGCAAT
CCGGATACGGAACAGGACGCAGCTTTGCTCGAAAAACTGGGATTGCATGCACTTTAA

Upstream 100 bases:

>100_bases
GTTCAAAATTCCAGAAGAAAAAATTGACAACCTAAAAGGTATCCGTAATGATCGCCTATCATTTATTATAACTTGGAGCA
GGCTATCGCCGAGAATGATC

Downstream 100 bases:

>100_bases
CCACCATGCTGCCGGACTTGGCTGAAGCGCTGCAACAGCGCAGGCAGGAAGGGTTGTATCGTTTTCGCCAGGTGCTGGAA
GGGCCGCAATCCCCGCGTGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MESRTDLISEKQCGCTYSNPDLAVQQESSSRWLVADVESLLGLPFNDLIFQAQTVHRQYHDANGVQLSTLLSVKTGGCSE
DCAYCPQAARYHTGVENQAILSREEVVMAATKAKESGATRFCMGAAWRGPKQRDIEHMTELISAVKDLGLETCATLGILK
PGQAVQLKQAGLDYYNHNLDTSPEFYGEVITTREYQNRLDTLDEVRGANINVCCGGIVGMGESRTARAGLIAQLASLDPY
PESVPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQALCFLAGANSIFYGDKLLTTGN
PDTEQDAALLEKLGLHAL

Sequences:

>Translated_338_residues
MESRTDLISEKQCGCTYSNPDLAVQQESSSRWLVADVESLLGLPFNDLIFQAQTVHRQYHDANGVQLSTLLSVKTGGCSE
DCAYCPQAARYHTGVENQAILSREEVVMAATKAKESGATRFCMGAAWRGPKQRDIEHMTELISAVKDLGLETCATLGILK
PGQAVQLKQAGLDYYNHNLDTSPEFYGEVITTREYQNRLDTLDEVRGANINVCCGGIVGMGESRTARAGLIAQLASLDPY
PESVPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQALCFLAGANSIFYGDKLLTTGN
PDTEQDAALLEKLGLHAL
>Mature_338_residues
MESRTDLISEKQCGCTYSNPDLAVQQESSSRWLVADVESLLGLPFNDLIFQAQTVHRQYHDANGVQLSTLLSVKTGGCSE
DCAYCPQAARYHTGVENQAILSREEVVMAATKAKESGATRFCMGAAWRGPKQRDIEHMTELISAVKDLGLETCATLGILK
PGQAVQLKQAGLDYYNHNLDTSPEFYGEVITTREYQNRLDTLDEVRGANINVCCGGIVGMGESRTARAGLIAQLASLDPY
PESVPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQALCFLAGANSIFYGDKLLTTGN
PDTEQDAALLEKLGLHAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=59.1503267973856, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=45.9375, Blast_Score=304, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_NITEC (Q0AE72)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748325.1
- ProteinModelPortal:   Q0AE72
- SMR:   Q0AE72
- STRING:   Q0AE72
- GeneID:   4274180
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2138
- NMPDR:   fig|335283.3.peg.173
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q0AE72
- ProtClustDB:   CLSK2298556
- BioCyc:   NEUT335283:NEUT_2138-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36855; Mature: 36855

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRTDLISEKQCGCTYSNPDLAVQQESSSRWLVADVESLLGLPFNDLIFQAQTVHRQYH
CCCHHHHHCCCCCCCCCCCCCCEEEECCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHC
DANGVQLSTLLSVKTGGCSEDCAYCPQAARYHTGVENQAILSREEVVMAATKAKESGATR
CCCCCEEEEEEEECCCCCCHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCE
FCMGAAWRGPKQRDIEHMTELISAVKDLGLETCATLGILKPGQAVQLKQAGLDYYNHNLD
EEHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCHHHCCCCC
TSPEFYGEVITTREYQNRLDTLDEVRGANINVCCGGIVGMGESRTARAGLIAQLASLDPY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHHHHHHCCCC
PESVPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQAL
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CFLAGANSIFYGDKLLTTGNPDTEQDAALLEKLGLHAL
HHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MESRTDLISEKQCGCTYSNPDLAVQQESSSRWLVADVESLLGLPFNDLIFQAQTVHRQYH
CCCHHHHHCCCCCCCCCCCCCCEEEECCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHC
DANGVQLSTLLSVKTGGCSEDCAYCPQAARYHTGVENQAILSREEVVMAATKAKESGATR
CCCCCEEEEEEEECCCCCCHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCE
FCMGAAWRGPKQRDIEHMTELISAVKDLGLETCATLGILKPGQAVQLKQAGLDYYNHNLD
EEHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCHHHCCCCC
TSPEFYGEVITTREYQNRLDTLDEVRGANINVCCGGIVGMGESRTARAGLIAQLASLDPY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHHHHHHCCCC
PESVPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQAL
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CFLAGANSIFYGDKLLTTGNPDTEQDAALLEKLGLHAL
HHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA