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The map label for this gene is bioH [H]

Identifier: 114332101

GI number: 114332101

Start: 2265174

End: 2265953

Strand: Reverse

Name: bioH [H]

Synonym: Neut_2136

Alternate gene names: 114332101

Gene position: 2265953-2265174 (Counterclockwise)

Preceding gene: 114332102

Following gene: 114332100

Centisome position: 85.15

GC content: 52.18

Gene sequence:

>780_bases
ATGATCTGGCTGGATGCTGATATGACCCGGTTGCATATTGAGACAAGCGGGAGCGGGCCTGATCTGGTGATGTTGCATGG
CTGGGCCATGCACAGTGGTGTATGGGAGTGTGTGTCAGAGCCGCTCTCCCGGCGTTTCCATCTGCACTGCATTGATTTGC
CGGGGCACGGTGCTAGCCGTGATTGTGCGCTGGATTCTCTGGAGCAGATGACCGAAGTGATTGCAGACCATTTGCCTGAT
AACAGCATTGTGTGCGGCTGGTCGCTCGGTGGGCAAGTGGCGATCAGGCTGGCATTACAGATGCCGGAACGGGTACAACA
ATTAGTACTGGTTGCCAGTACCCCTTGTTTCGTCAAACGTGCCAACTGGCCATGGGGGATGGAATCATTAACCCTGACCC
TGTTTATGGAAAATCTGGCACGTGACTATATGCAAACCCTCAATCGTTTCTTGACACTGCAAGTTAGCGGTAGCGAGGAT
CAGACCCGGGTACTGGCACGGCTGCGCAAGAGTATGTTGAGTGGTTTGTCACCGGAGTTTGCAACGTTGCAGGCAGGATT
AAAGATTCTGCAAACTAGTGATTTGCGAGCTGAACTGGATCAAATTAAACAGCCTGTGCTGCTGATTCATGGCCAGAATG
ATGTTATCGCGCCTGTTGGTGCTGCTGAATGGACGCAGCAGCACTTGTCCCAGGCGCAATTGAAGCTGTTTCCTCATTGC
GGCCACGCGCCGTTTCTTTCTTTTCCTGAGCAATTTGTGGGTTGTTTCGATGCTTTATGA

Upstream 100 bases:

>100_bases
ATTCGCCCTCCGACAGTGCCGCAAGGCATGGCACGGTTGCGCATCAGTTTGTCTGCGGCTCATGCTGGGGAAGATGTCGA
TCAATTGAGTGCAGCCTTGC

Downstream 100 bases:

>100_bases
TCATGTACTCGACAAGCGCATGTTGCGCCGGTCATTTGAACAGGCGGCGACCAGTTATGACCAGTCTGCCGTACTGCAGC
GGGAAATATGCGACCGTATG

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIWLDADMTRLHIETSGSGPDLVMLHGWAMHSGVWECVSEPLSRRFHLHCIDLPGHGASRDCALDSLEQMTEVIADHLPD
NSIVCGWSLGGQVAIRLALQMPERVQQLVLVASTPCFVKRANWPWGMESLTLTLFMENLARDYMQTLNRFLTLQVSGSED
QTRVLARLRKSMLSGLSPEFATLQAGLKILQTSDLRAELDQIKQPVLLIHGQNDVIAPVGAAEWTQQHLSQAQLKLFPHC
GHAPFLSFPEQFVGCFDAL

Sequences:

>Translated_259_residues
MIWLDADMTRLHIETSGSGPDLVMLHGWAMHSGVWECVSEPLSRRFHLHCIDLPGHGASRDCALDSLEQMTEVIADHLPD
NSIVCGWSLGGQVAIRLALQMPERVQQLVLVASTPCFVKRANWPWGMESLTLTLFMENLARDYMQTLNRFLTLQVSGSED
QTRVLARLRKSMLSGLSPEFATLQAGLKILQTSDLRAELDQIKQPVLLIHGQNDVIAPVGAAEWTQQHLSQAQLKLFPHC
GHAPFLSFPEQFVGCFDAL
>Mature_259_residues
MIWLDADMTRLHIETSGSGPDLVMLHGWAMHSGVWECVSEPLSRRFHLHCIDLPGHGASRDCALDSLEQMTEVIADHLPD
NSIVCGWSLGGQVAIRLALQMPERVQQLVLVASTPCFVKRANWPWGMESLTLTLFMENLARDYMQTLNRFLTLQVSGSED
QTRVLARLRKSMLSGLSPEFATLQAGLKILQTSDLRAELDQIKQPVLLIHGQNDVIAPVGAAEWTQQHLSQAQLKLFPHC
GHAPFLSFPEQFVGCFDAL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=259, Percent_Identity=25.4826254826255, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1789817, Length=253, Percent_Identity=40.3162055335968, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28877; Mature: 28877

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWLDADMTRLHIETSGSGPDLVMLHGWAMHSGVWECVSEPLSRRFHLHCIDLPGHGASR
CEEECCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DCALDSLEQMTEVIADHLPDNSIVCGWSLGGQVAIRLALQMPERVQQLVLVASTPCFVKR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHCCCCCEEEE
ANWPWGMESLTLTLFMENLARDYMQTLNRFLTLQVSGSEDQTRVLARLRKSMLSGLSPEF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
ATLQAGLKILQTSDLRAELDQIKQPVLLIHGQNDVIAPVGAAEWTQQHLSQAQLKLFPHC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCC
GHAPFLSFPEQFVGCFDAL
CCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIWLDADMTRLHIETSGSGPDLVMLHGWAMHSGVWECVSEPLSRRFHLHCIDLPGHGASR
CEEECCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DCALDSLEQMTEVIADHLPDNSIVCGWSLGGQVAIRLALQMPERVQQLVLVASTPCFVKR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHCCCCCEEEE
ANWPWGMESLTLTLFMENLARDYMQTLNRFLTLQVSGSEDQTRVLARLRKSMLSGLSPEF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
ATLQAGLKILQTSDLRAELDQIKQPVLLIHGQNDVIAPVGAAEWTQQHLSQAQLKLFPHC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCC
GHAPFLSFPEQFVGCFDAL
CCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]