Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 114332048

GI number: 114332048

Start: 2203488

End: 2204210

Strand: Reverse

Name: phoB [H]

Synonym: Neut_2082

Alternate gene names: 114332048

Gene position: 2204210-2203488 (Counterclockwise)

Preceding gene: 114332049

Following gene: 114332047

Centisome position: 82.83

GC content: 48.69

Gene sequence:

>723_bases
ATGTCTGCAACGGTTTTGGTGGTAGAAGATGAGCCAGCAATTCAGGAGTTGATTGCCTATAATCTGCGCAATGCGAACTA
TATTGTTGTATGTGTAAATAGTGCCGAACAAGCGGTAGTAAAGATTAATGAGATATTGCCGGACCTGATATTACTGGACT
GGATGCTCCTGGGTAAAAGTGGCATCGAATTTGCGCGATCACTGCGCCGAGATCCGCGTACCAGGTCGATTCCTGTCATT
ATGCTGACTGCCCGGGTAGAGGAAGGTGACAAGGTGTCTGGGCTGGAAGCAGGAGCGGATGATTACATTACCAAACCATT
TTCACCACGCGAGCTGATCGCCCGGATAAAGGCAGTTTTGCGTAGGAGTCTGCCCGAAGCTTCTGATGAAATTCTCAATG
CTGAGGGAGTGAGGCTGAACCCGATGACGCATCGGGTAACAGTCATTAATCAGGAAGATGGATTTCGAGAAAAGGAATTA
CTGGTCAGTCCCACTGAATTTCGATTGCTGCATTTTCTGATGGCTCATGCTGAGCGTGTGCATACTCGCGCCCAGTTATT
AGATCGGGTATGGGGTGATCATGTTTTTGTCGAAGATCGGACGGTTGACGTTCACATACGCCGGTTGCGCAAGGTGCTGG
AGGTTGCTGGAAAAGCTGATTTGATCCAGACAGTAAGAGGGGCCGGGTATCGTTTTTCCACGCGAAATGAAGATGCTTTC
TGA

Upstream 100 bases:

>100_bases
CTATATTGTCAACAAAACAAGTTTTCAGGAAGTCATTGCTATGTCATCATTGCCTTGCATAATTCGTGCTGTTAGCCATA
TCCCCTCAGTGAGATGAAAG

Downstream 100 bases:

>100_bases
AATTGGGATGCGCTGGCCAATGAGATATCGGTAAGTTATACTGCCTTGACAAGCGCCATAGGTGGATGAGCCATATTAAT
GGCAATTGCAGGGCGCTTTT

Product: two component transcriptional regulator, winged helix family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSATVLVVEDEPAIQELIAYNLRNANYIVVCVNSAEQAVVKINEILPDLILLDWMLLGKSGIEFARSLRRDPRTRSIPVI
MLTARVEEGDKVSGLEAGADDYITKPFSPRELIARIKAVLRRSLPEASDEILNAEGVRLNPMTHRVTVINQEDGFREKEL
LVSPTEFRLLHFLMAHAERVHTRAQLLDRVWGDHVFVEDRTVDVHIRRLRKVLEVAGKADLIQTVRGAGYRFSTRNEDAF

Sequences:

>Translated_240_residues
MSATVLVVEDEPAIQELIAYNLRNANYIVVCVNSAEQAVVKINEILPDLILLDWMLLGKSGIEFARSLRRDPRTRSIPVI
MLTARVEEGDKVSGLEAGADDYITKPFSPRELIARIKAVLRRSLPEASDEILNAEGVRLNPMTHRVTVINQEDGFREKEL
LVSPTEFRLLHFLMAHAERVHTRAQLLDRVWGDHVFVEDRTVDVHIRRLRKVLEVAGKADLIQTVRGAGYRFSTRNEDAF
>Mature_239_residues
SATVLVVEDEPAIQELIAYNLRNANYIVVCVNSAEQAVVKINEILPDLILLDWMLLGKSGIEFARSLRRDPRTRSIPVIM
LTARVEEGDKVSGLEAGADDYITKPFSPRELIARIKAVLRRSLPEASDEILNAEGVRLNPMTHRVTVINQEDGFREKELL
VSPTEFRLLHFLMAHAERVHTRAQLLDRVWGDHVFVEDRTVDVHIRRLRKVLEVAGKADLIQTVRGAGYRFSTRNEDAF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=235, Percent_Identity=56.5957446808511, Blast_Score=267, Evalue=4e-73,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=40.0862068965517, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=38.6266094420601, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789809, Length=236, Percent_Identity=35.5932203389831, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786911, Length=231, Percent_Identity=36.7965367965368, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082012, Length=228, Percent_Identity=34.6491228070175, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790863, Length=240, Percent_Identity=35, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=37.1794871794872, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787375, Length=231, Percent_Identity=31.6017316017316, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=31.4159292035398, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1789402, Length=231, Percent_Identity=31.1688311688312, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790552, Length=234, Percent_Identity=32.9059829059829, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=238, Percent_Identity=32.7731092436975, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI145693140, Length=242, Percent_Identity=33.4710743801653, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27247; Mature: 27116

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVLVVEDEPAIQELIAYNLRNANYIVVCVNSAEQAVVKINEILPDLILLDWMLLGKS
CCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
GIEFARSLRRDPRTRSIPVIMLTARVEEGDKVSGLEAGADDYITKPFSPRELIARIKAVL
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRSLPEASDEILNAEGVRLNPMTHRVTVINQEDGFREKELLVSPTEFRLLHFLMAHAERV
HHHCCCHHHHHHCCCCEEECCCEEEEEEEECCCCCCCHHHEECCHHHHHHHHHHHHHHHH
HTRAQLLDRVWGDHVFVEDRTVDVHIRRLRKVLEVAGKADLIQTVRGAGYRFSTRNEDAF
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCCC
>Mature Secondary Structure 
SATVLVVEDEPAIQELIAYNLRNANYIVVCVNSAEQAVVKINEILPDLILLDWMLLGKS
CEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
GIEFARSLRRDPRTRSIPVIMLTARVEEGDKVSGLEAGADDYITKPFSPRELIARIKAVL
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRSLPEASDEILNAEGVRLNPMTHRVTVINQEDGFREKELLVSPTEFRLLHFLMAHAERV
HHHCCCHHHHHHCCCCEEECCCEEEEEEEECCCCCCCHHHEECCHHHHHHHHHHHHHHHH
HTRAQLLDRVWGDHVFVEDRTVDVHIRRLRKVLEVAGKADLIQTVRGAGYRFSTRNEDAF
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]