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The map label for this gene is glcE [H]

Identifier: 114331858

GI number: 114331858

Start: 1998049

End: 1999149

Strand: Direct

Name: glcE [H]

Synonym: Neut_1883

Alternate gene names: 114331858

Gene position: 1998049-1999149 (Clockwise)

Preceding gene: 114331857

Following gene: 114331859

Centisome position: 75.08

GC content: 55.4

Gene sequence:

>1101_bases
ATGCAAGATCAAACCATTCTCGATCAGCTCATCCAGACCGTTACAGAAGCAGCCGAATATAAACAGGCGTTATGCATCCG
GGGAGGCGGCAGCAAGGATTTTTACGGCAACCCGCAGGCGTTGCAACCTCCCGCGCTCAACACAGCTACCTGGCAGGGAA
TCGTCGATTATGAACCCAGTGAGTTGGTTATCACTGCTCGCGCCGGAACACCGCTGGCCGAGCTGGAGAAGCTGCTGCAT
GATCATGGTCAGATGCTGGCTTTTGAACCTCCTTGCTTCAATACATCCGCTACGCTGGGCGGCTGTGTCGCTGCCGGATT
ATCCGGCCCCAGACGCGCCTACACCGGCGCAGTACGCGACTATGTATTAGGTATCGGATTGCTGGATGGCCAGGGCAGAA
CCCTGTCTTTTGGGGGGCGTGTCATGAAGAACGTGGCCGGTTATGATGTCTCCCGCCTGATGACAGGCGCCATGGGCACG
CTGGGGGTATTGCTCGAAGTGTCACTTAAGGTACTCCCTAAGCCGGCTGTAGAACGCACCCTGTGCATTCAGACCAACAT
GCCCCAAGCGATCGGAATCATGCGCCGCTGTGCAGCTGAACCGCTGCCGGTTTCCGCTACCTGTTTTCATGAGAATCAGC
TTTATGTCCGGCTTTCCGGTGCTGAATCCGCCGTGCACACGGCACATGCCAGACTGGGCGGAGATGAAATTAAAGATAAG
GGAAGTTGTGAGGAAAATTTCTGGAAATCCGTCAGGGATCACACCCATCCGTTTTTCCAGCAAGGAAAATCGAATGGCAA
ATCGCTGTGGCGGCTGTCGATCCAATCGACCACCCCTCCGCTTTCGCTGCCAGGTGAACAACTGATTGAATGGGGCGGTG
CACTGCGCTGGCTGGTGACAGACGAAGATACCGATGCTGCCAAGATCCGCAACCTTGCTTCCGGTGCCGGCGGCCATGCC
ACTCTACTCCATGGCAATAAAACCGCAACCCCCGTATTTCACCCGCTTTCCCCTGCCCTGCTTAAAATCCACCAGAGACT
GAAGCAGCAGTTTGATCCGGCAGGTATTCTGAATCCGCAACGACTGTATGCCGAGTTTTAA

Upstream 100 bases:

>100_bases
GCACCGTTGCGCCGAATTCGGGGCGATGCATGTCCATCGAGGCGCTGAAAAATTTCCAGACATACCCAGGTTCTAATCAG
ACATACCCAAGTTCTAATTT

Downstream 100 bases:

>100_bases
ACCATGCAAACCCGTTTGACTGATCTGATTAAAGACACCCCGGAAGGCAAGGAAGCCGATGCCATTCTGCGCAGTTGTGT
GCATTGTGGCTTCTGCCTTG

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQDQTILDQLIQTVTEAAEYKQALCIRGGGSKDFYGNPQALQPPALNTATWQGIVDYEPSELVITARAGTPLAELEKLLH
DHGQMLAFEPPCFNTSATLGGCVAAGLSGPRRAYTGAVRDYVLGIGLLDGQGRTLSFGGRVMKNVAGYDVSRLMTGAMGT
LGVLLEVSLKVLPKPAVERTLCIQTNMPQAIGIMRRCAAEPLPVSATCFHENQLYVRLSGAESAVHTAHARLGGDEIKDK
GSCEENFWKSVRDHTHPFFQQGKSNGKSLWRLSIQSTTPPLSLPGEQLIEWGGALRWLVTDEDTDAAKIRNLASGAGGHA
TLLHGNKTATPVFHPLSPALLKIHQRLKQQFDPAGILNPQRLYAEF

Sequences:

>Translated_366_residues
MQDQTILDQLIQTVTEAAEYKQALCIRGGGSKDFYGNPQALQPPALNTATWQGIVDYEPSELVITARAGTPLAELEKLLH
DHGQMLAFEPPCFNTSATLGGCVAAGLSGPRRAYTGAVRDYVLGIGLLDGQGRTLSFGGRVMKNVAGYDVSRLMTGAMGT
LGVLLEVSLKVLPKPAVERTLCIQTNMPQAIGIMRRCAAEPLPVSATCFHENQLYVRLSGAESAVHTAHARLGGDEIKDK
GSCEENFWKSVRDHTHPFFQQGKSNGKSLWRLSIQSTTPPLSLPGEQLIEWGGALRWLVTDEDTDAAKIRNLASGAGGHA
TLLHGNKTATPVFHPLSPALLKIHQRLKQQFDPAGILNPQRLYAEF
>Mature_366_residues
MQDQTILDQLIQTVTEAAEYKQALCIRGGGSKDFYGNPQALQPPALNTATWQGIVDYEPSELVITARAGTPLAELEKLLH
DHGQMLAFEPPCFNTSATLGGCVAAGLSGPRRAYTGAVRDYVLGIGLLDGQGRTLSFGGRVMKNVAGYDVSRLMTGAMGT
LGVLLEVSLKVLPKPAVERTLCIQTNMPQAIGIMRRCAAEPLPVSATCFHENQLYVRLSGAESAVHTAHARLGGDEIKDK
GSCEENFWKSVRDHTHPFFQQGKSNGKSLWRLSIQSTTPPLSLPGEQLIEWGGALRWLVTDEDTDAAKIRNLASGAGGHA
TLLHGNKTATPVFHPLSPALLKIHQRLKQQFDPAGILNPQRLYAEF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=357, Percent_Identity=46.218487394958, Blast_Score=313, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6320027, Length=127, Percent_Identity=30.7086614173228, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24653753, Length=214, Percent_Identity=27.1028037383178, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 39587; Mature: 39587

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQTILDQLIQTVTEAAEYKQALCIRGGGSKDFYGNPQALQPPALNTATWQGIVDYEPS
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
ELVITARAGTPLAELEKLLHDHGQMLAFEPPCFNTSATLGGCVAAGLSGPRRAYTGAVRD
EEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
YVLGIGLLDGQGRTLSFGGRVMKNVAGYDVSRLMTGAMGTLGVLLEVSLKVLPKPAVERT
HHHEEEEECCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCE
LCIQTNMPQAIGIMRRCAAEPLPVSATCFHENQLYVRLSGAESAVHTAHARLGGDEIKDK
EEEECCCCHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHCCCHHCCCC
GSCEENFWKSVRDHTHPFFQQGKSNGKSLWRLSIQSTTPPLSLPGEQLIEWGGALRWLVT
CCCHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCEEEEEEE
DEDTDAAKIRNLASGAGGHATLLHGNKTATPVFHPLSPALLKIHQRLKQQFDPAGILNPQ
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
RLYAEF
HHHHCC
>Mature Secondary Structure
MQDQTILDQLIQTVTEAAEYKQALCIRGGGSKDFYGNPQALQPPALNTATWQGIVDYEPS
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
ELVITARAGTPLAELEKLLHDHGQMLAFEPPCFNTSATLGGCVAAGLSGPRRAYTGAVRD
EEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
YVLGIGLLDGQGRTLSFGGRVMKNVAGYDVSRLMTGAMGTLGVLLEVSLKVLPKPAVERT
HHHEEEEECCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCE
LCIQTNMPQAIGIMRRCAAEPLPVSATCFHENQLYVRLSGAESAVHTAHARLGGDEIKDK
EEEECCCCHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHCCCHHCCCC
GSCEENFWKSVRDHTHPFFQQGKSNGKSLWRLSIQSTTPPLSLPGEQLIEWGGALRWLVT
CCCHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCEEEEEEE
DEDTDAAKIRNLASGAGGHATLLHGNKTATPVFHPLSPALLKIHQRLKQQFDPAGILNPQ
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
RLYAEF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]