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The map label for this gene is glcF [H]

Identifier: 114331859

GI number: 114331859

Start: 1999153

End: 2000415

Strand: Direct

Name: glcF [H]

Synonym: Neut_1884

Alternate gene names: 114331859

Gene position: 1999153-2000415 (Clockwise)

Preceding gene: 114331858

Following gene: 114331865

Centisome position: 75.13

GC content: 55.9

Gene sequence:

>1263_bases
ATGCAAACCCGTTTGACTGATCTGATTAAAGACACCCCGGAAGGCAAGGAAGCCGATGCCATTCTGCGCAGTTGTGTGCA
TTGTGGCTTCTGCCTTGCTACCTGCCCCACCTACCAACTGCTGGGAAACGAGCTGGATAGCCCGCGTGGGCGAATTTATC
TGATCAAGCAGATGCTGGAAGGCCAGGAGGTAACAGAAAAAACGCAGCTGCATCTCGATCGCTGCCTGACCTGTCGCGCC
TGCGAAACCACCTGTCCATCCGGGGTACAGTACGGGCGTCTGCTTGATATCGGCCGGGGCATGGTCGAACAGCAAATCCA
GCGTACGCCTTATGCAACATTCAAGCGCTATACCTTGCGCAAACTGCTGCCCAACCGTACTCTCTCCAGCTTTTTCATGG
GCGTTGCCCGCTTGAGTCGCCCGCTGCTCCCCGCCCGGCTGAAAAGAACGATCTTACCGAAGCCGGTAACTGCACGCAGC
CTCTCCGGCAAAACCCACACACGCTCCATGCTGATACTCGCTGGTTGTGTACAGCCGGCCATGACACCCAACACCAACCA
CGCTACCACACGCGTGCTGGACAGGCTCGGCATCTCGGTCATCACCGCGGAACAGGCCGGCTGCTGCGGTGCACTGGCTT
ATCACCTTAATGCGCAGGAAGAAGGATTGAACGCCATGCGCCGCAATATCGATGCATGGTGGCCTTTTGTCACGCGATCC
GAGAATCCGGTTGAGGCCATTATCGTCACGGCCAGCGGCTGCGGCGTCACTGTCAAGGATTACGGACATCTGCTGCAACA
CGACCCAGTTTATGCCGAGAAAGCAGAACGAATTTCCAGCCTGACCCGCGACATCAGTGAAATCATCACGGCCGAAACCT
CCACACTGATTCCGTTGTTGGAAGCAGCGAAAACCGCATCGAAATCCTCCTCGTTCCCACGCAACCTTGCCTTTCATACC
CCCTGCACCTTACAACATGGCATGAAACTGAAAGGAAAAGTCGAACCGATTCTGCAAGCAGCGGGCTTTAACCTCACCAA
AGTCGCTGACCCCCATCTCTGCTGCGGTTCAGCGGGCACTTATTCCATCCTGCAACCCAAACTTTCTCGTCAGCTACGTG
ATAACAAGATTACTGCATTGACCATGGAAAACCCCGATCTGGTGGTTACCGCCAATATCGGCTGCCAGATGCACCTGCAA
GGCGGCACCCGGCTGCCTGTACGCCACTGGATTGAACTGCTGGATGAAACCCTGGGCGGATAA

Upstream 100 bases:

>100_bases
GCTTTCCCCTGCCCTGCTTAAAATCCACCAGAGACTGAAGCAGCAGTTTGATCCGGCAGGTATTCTGAATCCGCAACGAC
TGTATGCCGAGTTTTAAACC

Downstream 100 bases:

>100_bases
TCCCCAAATATCAGGGAAACGCTCTTTATCCGCGCGGCCGCACCGCCGCCAGCGGCAATTGATTCAGCTCCGCCGTGGCC
TCGCGCCAGGCTGGATAATA

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQTRLTDLIKDTPEGKEADAILRSCVHCGFCLATCPTYQLLGNELDSPRGRIYLIKQMLEGQEVTEKTQLHLDRCLTCRA
CETTCPSGVQYGRLLDIGRGMVEQQIQRTPYATFKRYTLRKLLPNRTLSSFFMGVARLSRPLLPARLKRTILPKPVTARS
LSGKTHTRSMLILAGCVQPAMTPNTNHATTRVLDRLGISVITAEQAGCCGALAYHLNAQEEGLNAMRRNIDAWWPFVTRS
ENPVEAIIVTASGCGVTVKDYGHLLQHDPVYAEKAERISSLTRDISEIITAETSTLIPLLEAAKTASKSSSFPRNLAFHT
PCTLQHGMKLKGKVEPILQAAGFNLTKVADPHLCCGSAGTYSILQPKLSRQLRDNKITALTMENPDLVVTANIGCQMHLQ
GGTRLPVRHWIELLDETLGG

Sequences:

>Translated_420_residues
MQTRLTDLIKDTPEGKEADAILRSCVHCGFCLATCPTYQLLGNELDSPRGRIYLIKQMLEGQEVTEKTQLHLDRCLTCRA
CETTCPSGVQYGRLLDIGRGMVEQQIQRTPYATFKRYTLRKLLPNRTLSSFFMGVARLSRPLLPARLKRTILPKPVTARS
LSGKTHTRSMLILAGCVQPAMTPNTNHATTRVLDRLGISVITAEQAGCCGALAYHLNAQEEGLNAMRRNIDAWWPFVTRS
ENPVEAIIVTASGCGVTVKDYGHLLQHDPVYAEKAERISSLTRDISEIITAETSTLIPLLEAAKTASKSSSFPRNLAFHT
PCTLQHGMKLKGKVEPILQAAGFNLTKVADPHLCCGSAGTYSILQPKLSRQLRDNKITALTMENPDLVVTANIGCQMHLQ
GGTRLPVRHWIELLDETLGG
>Mature_420_residues
MQTRLTDLIKDTPEGKEADAILRSCVHCGFCLATCPTYQLLGNELDSPRGRIYLIKQMLEGQEVTEKTQLHLDRCLTCRA
CETTCPSGVQYGRLLDIGRGMVEQQIQRTPYATFKRYTLRKLLPNRTLSSFFMGVARLSRPLLPARLKRTILPKPVTARS
LSGKTHTRSMLILAGCVQPAMTPNTNHATTRVLDRLGISVITAEQAGCCGALAYHLNAQEEGLNAMRRNIDAWWPFVTRS
ENPVEAIIVTASGCGVTVKDYGHLLQHDPVYAEKAERISSLTRDISEIITAETSTLIPLLEAAKTASKSSSFPRNLAFHT
PCTLQHGMKLKGKVEPILQAAGFNLTKVADPHLCCGSAGTYSILQPKLSRQLRDNKITALTMENPDLVVTANIGCQMHLQ
GGTRLPVRHWIELLDETLGG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=418, Percent_Identity=54.7846889952153, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786497, Length=245, Percent_Identity=23.265306122449, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46300; Mature: 46300

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRLTDLIKDTPEGKEADAILRSCVHCGFCLATCPTYQLLGNELDSPRGRIYLIKQMLE
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHC
GQEVTEKTQLHLDRCLTCRACETTCPSGVQYGRLLDIGRGMVEQQIQRTPYATFKRYTLR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KLLPNRTLSSFFMGVARLSRPLLPARLKRTILPKPVTARSLSGKTHTRSMLILAGCVQPA
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
MTPNTNHATTRVLDRLGISVITAEQAGCCGALAYHLNAQEEGLNAMRRNIDAWWPFVTRS
CCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCHHHCCEEECC
ENPVEAIIVTASGCGVTVKDYGHLLQHDPVYAEKAERISSLTRDISEIITAETSTLIPLL
CCCEEEEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
EAAKTASKSSSFPRNLAFHTPCTLQHGMKLKGKVEPILQAAGFNLTKVADPHLCCGSAGT
HHHHHHHCCCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
YSILQPKLSRQLRDNKITALTMENPDLVVTANIGCQMHLQGGTRLPVRHWIELLDETLGG
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTRLTDLIKDTPEGKEADAILRSCVHCGFCLATCPTYQLLGNELDSPRGRIYLIKQMLE
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHC
GQEVTEKTQLHLDRCLTCRACETTCPSGVQYGRLLDIGRGMVEQQIQRTPYATFKRYTLR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KLLPNRTLSSFFMGVARLSRPLLPARLKRTILPKPVTARSLSGKTHTRSMLILAGCVQPA
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
MTPNTNHATTRVLDRLGISVITAEQAGCCGALAYHLNAQEEGLNAMRRNIDAWWPFVTRS
CCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCHHHCCEEECC
ENPVEAIIVTASGCGVTVKDYGHLLQHDPVYAEKAERISSLTRDISEIITAETSTLIPLL
CCCEEEEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
EAAKTASKSSSFPRNLAFHTPCTLQHGMKLKGKVEPILQAAGFNLTKVADPHLCCGSAGT
HHHHHHHCCCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
YSILQPKLSRQLRDNKITALTMENPDLVVTANIGCQMHLQGGTRLPVRHWIELLDETLGG
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]