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The map label for this gene is ogt [H]

Identifier: 114331218

GI number: 114331218

Start: 1277813

End: 1278316

Strand: Reverse

Name: ogt [H]

Synonym: Neut_1220

Alternate gene names: 114331218

Gene position: 1278316-1277813 (Counterclockwise)

Preceding gene: 114331219

Following gene: 114331213

Centisome position: 48.04

GC content: 44.44

Gene sequence:

>504_bases
ATGAATTATTACATCATTATGGAAAGTCCCATTGATCCGTTGTTGCTCACGAGTGATGGTGAATTTCTTACTGGGTTATA
TATGGAAAAGGAAGCTCAGAAACTGTTATCCGGCATGAAAGATGGCTGGCTACAGAATACAGATGCTATCCCTTTCAAAG
AAGCTATTGCCCAGTTAAATGCTTATTTTTCCGGGGAACTGCATGAATTTGATTTGCCACTCAAAGCAACTGGCACCCCT
TTTCAGGAAATGGTCTGGCAGGCATTAACCTCTATTCCTTATGGCAAGACAGTTAGTTACAAAGATATTGCCGAGCACGT
TCATCTGCCCAGAGCTGCACGTGCGGTAGGAATGGCAAACGGTCGTAACCCTATTTCCATTATTATTCCCTGTCATCGCG
TCATTGGCGCCAACGGCAAACTAACGGGGTACGGAGGCGGTATCCACCGCAAGGAGTGGCTGCTTGAACATGAACATAAA
GATAAGCAAACTACTCTTATCTGA

Upstream 100 bases:

>100_bases
ATGGTGCGATTCACTTATGGCATCAGTGTGCAGGAGAATGAGAAAATAGCGCTATTAAGTATAGTCTGATTACATGATAA
TCCTACAAGGAGAGCAAGCA

Downstream 100 bases:

>100_bases
TTTTATGGATGTGGCGGGTAATTGCTGAATATTGCTCAAGCGATAAATTTTCTGCCCGCAGCCCCGGATCAATCCTCAGC
TGGTCAAAATCCTTGATAGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MNYYIIMESPIDPLLLTSDGEFLTGLYMEKEAQKLLSGMKDGWLQNTDAIPFKEAIAQLNAYFSGELHEFDLPLKATGTP
FQEMVWQALTSIPYGKTVSYKDIAEHVHLPRAARAVGMANGRNPISIIIPCHRVIGANGKLTGYGGGIHRKEWLLEHEHK
DKQTTLI

Sequences:

>Translated_167_residues
MNYYIIMESPIDPLLLTSDGEFLTGLYMEKEAQKLLSGMKDGWLQNTDAIPFKEAIAQLNAYFSGELHEFDLPLKATGTP
FQEMVWQALTSIPYGKTVSYKDIAEHVHLPRAARAVGMANGRNPISIIIPCHRVIGANGKLTGYGGGIHRKEWLLEHEHK
DKQTTLI
>Mature_167_residues
MNYYIIMESPIDPLLLTSDGEFLTGLYMEKEAQKLLSGMKDGWLQNTDAIPFKEAIAQLNAYFSGELHEFDLPLKATGTP
FQEMVWQALTSIPYGKTVSYKDIAEHVHLPRAARAVGMANGRNPISIIIPCHRVIGANGKLTGYGGGIHRKEWLLEHEHK
DKQTTLI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=115, Percent_Identity=40, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=159, Percent_Identity=43.3962264150943, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1788542, Length=111, Percent_Identity=45.045045045045, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=97, Percent_Identity=43.298969072165, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=48.75, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=45.2380952380952, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18696; Mature: 18696

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00374 MGMT ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYIIMESPIDPLLLTSDGEFLTGLYMEKEAQKLLSGMKDGWLQNTDAIPFKEAIAQLN
CCEEEEEECCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AYFSGELHEFDLPLKATGTPFQEMVWQALTSIPYGKTVSYKDIAEHVHLPRAARAVGMAN
HHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHCCC
GRNPISIIIPCHRVIGANGKLTGYGGGIHRKEWLLEHEHKDKQTTLI
CCCCEEEEEECHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNYYIIMESPIDPLLLTSDGEFLTGLYMEKEAQKLLSGMKDGWLQNTDAIPFKEAIAQLN
CCEEEEEECCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AYFSGELHEFDLPLKATGTPFQEMVWQALTSIPYGKTVSYKDIAEHVHLPRAARAVGMAN
HHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHCCC
GRNPISIIIPCHRVIGANGKLTGYGGGIHRKEWLLEHEHKDKQTTLI
CCCCEEEEEECHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]