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The map label for this gene is ada [H]

Identifier: 114331219

GI number: 114331219

Start: 1278376

End: 1279809

Strand: Reverse

Name: ada [H]

Synonym: Neut_1221

Alternate gene names: 114331219

Gene position: 1279809-1278376 (Counterclockwise)

Preceding gene: 114331223

Following gene: 114331218

Centisome position: 48.09

GC content: 47.77

Gene sequence:

>1434_bases
TTGGATTCTGTACGTTGTTATGCGGTACTTCAGGCGCGCGACACTAATTCTGATGGTTTGTTTTTTGTAGGTGTATCCAC
TACACGGATTTATTGCCGTCCCGTTTGTCGAGCACGTTTGCCACAAGTAGATTGGTGTACATTTTATTCTAGCGCAGCAC
GTGCGGAGCAGGATGGTTACCGTCCTTGTTTGCGTTGTCGGCCTGAACTTGCACCGGGCAATCTCAGAACAAGTGGAATC
GGATACGAGGTTCAGTCGACAGCAGAACGAATCCGGATGGGATTATTGATGCACAATAATATCGAAACATTGATGGATGA
GCTTGGGCTGCAGTCATATCAATTCCACCAGATCGTTGAACGTGAATATGGCGTAAACGCGACTGAGCTGGAAACAACCC
AGCGTCTGTTGCTGGCTAAACAATTACTCACGGATACAACTCTGGAAATAAATGATATAGCGCACACCTGTGGTTTTCCA
AGTGTTCCCCATTTTAGCGATACCTTTACATCCAGATACCGGTTAAATCCATTATCCCTACGCAAGAAATATCCGGTCAA
CGAGGAAACTACTATTTTGTTACGGCTGAGTTATCGCCCACCGCTGGCATGGAATGCCTTAATCCGTTTTCTCTGTAGTC
GCAGTAATCTACGCTTGAGTCAGATACAGAATGGCAACTATCTGCAAACAGTAAATCTTGATGGATGTCAGGGCTGGGTT
ACTGCAAAACATGACACAAAGCGCCATCAGATATATGTGCAAGCCTCACGGTCTTTATTACCTTGTCTTATCCGGTTGCA
AATGTATCTACGCCGTCTATTTGATTTGGACGCTAATCCGGCAATAATCGAAGCTCATCTGGGAAATGACGACATATTAA
AGCCGCTGATTGCCAATCATCCCGGTCTGCGTATCCCCGGAACATTGGATATTTTTGAACTGGGTCTACGCGCAATACTG
GGGCAACAGATAACCGTCAAGGCAGCCACTACATTGTTTGGCCGCTTTGTTGCAACCTTTGGTAAGCCAGTGGACACCCC
TTTCCCGGGGCTGGATCGTACTAGTCCGCCTGCAGAGCTCATCGCCGACACTTCTCTGCAAGCCCTCATCGATATTGGTC
TGACTGGGCGGCGAGCTCTGACTATTCAGCGATTTGCACAAACTATCGTGAATGGCGCACTGAAACCTGAGTCAATCGAT
CGAAACAAAATAATCGAACAACTACTGGAACTGCCGGGGATTGGCCCCTGGACTGCTCAATACATTGCAATACGGGCACT
GGGGGACTCCAATGCATTTCCAGCTTCAGATCTCGGTCTGTTACGCGGATTGCGGATGGAAAAACCGGCCGAACTGTTGC
GTCGTACTGAAAAATGGCAACCTTGGCGTGCATATGGTGCGATTCACTTATGGCATCAGTGTGCAGGAGAATGA

Upstream 100 bases:

>100_bases
TATGTTAAATTAAAGAAATAAATCTGGAAAAAGGGAATAATAATGCGTTTCTTACTCCCGCAACTTTCTTCATGGCAGCC
CTCTCGTGAACATTCGTCCT

Downstream 100 bases:

>100_bases
GAAAATAGCGCTATTAAGTATAGTCTGATTACATGATAATCCTACAAGGAGAGCAAGCAATGAATTATTACATCATTATG
GAAAGTCCCATTGATCCGTT

Product: transcriptional regulator, AraC family protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDSVRCYAVLQARDTNSDGLFFVGVSTTRIYCRPVCRARLPQVDWCTFYSSAARAEQDGYRPCLRCRPELAPGNLRTSGI
GYEVQSTAERIRMGLLMHNNIETLMDELGLQSYQFHQIVEREYGVNATELETTQRLLLAKQLLTDTTLEINDIAHTCGFP
SVPHFSDTFTSRYRLNPLSLRKKYPVNEETTILLRLSYRPPLAWNALIRFLCSRSNLRLSQIQNGNYLQTVNLDGCQGWV
TAKHDTKRHQIYVQASRSLLPCLIRLQMYLRRLFDLDANPAIIEAHLGNDDILKPLIANHPGLRIPGTLDIFELGLRAIL
GQQITVKAATTLFGRFVATFGKPVDTPFPGLDRTSPPAELIADTSLQALIDIGLTGRRALTIQRFAQTIVNGALKPESID
RNKIIEQLLELPGIGPWTAQYIAIRALGDSNAFPASDLGLLRGLRMEKPAELLRRTEKWQPWRAYGAIHLWHQCAGE

Sequences:

>Translated_477_residues
MDSVRCYAVLQARDTNSDGLFFVGVSTTRIYCRPVCRARLPQVDWCTFYSSAARAEQDGYRPCLRCRPELAPGNLRTSGI
GYEVQSTAERIRMGLLMHNNIETLMDELGLQSYQFHQIVEREYGVNATELETTQRLLLAKQLLTDTTLEINDIAHTCGFP
SVPHFSDTFTSRYRLNPLSLRKKYPVNEETTILLRLSYRPPLAWNALIRFLCSRSNLRLSQIQNGNYLQTVNLDGCQGWV
TAKHDTKRHQIYVQASRSLLPCLIRLQMYLRRLFDLDANPAIIEAHLGNDDILKPLIANHPGLRIPGTLDIFELGLRAIL
GQQITVKAATTLFGRFVATFGKPVDTPFPGLDRTSPPAELIADTSLQALIDIGLTGRRALTIQRFAQTIVNGALKPESID
RNKIIEQLLELPGIGPWTAQYIAIRALGDSNAFPASDLGLLRGLRMEKPAELLRRTEKWQPWRAYGAIHLWHQCAGE
>Mature_477_residues
MDSVRCYAVLQARDTNSDGLFFVGVSTTRIYCRPVCRARLPQVDWCTFYSSAARAEQDGYRPCLRCRPELAPGNLRTSGI
GYEVQSTAERIRMGLLMHNNIETLMDELGLQSYQFHQIVEREYGVNATELETTQRLLLAKQLLTDTTLEINDIAHTCGFP
SVPHFSDTFTSRYRLNPLSLRKKYPVNEETTILLRLSYRPPLAWNALIRFLCSRSNLRLSQIQNGNYLQTVNLDGCQGWV
TAKHDTKRHQIYVQASRSLLPCLIRLQMYLRRLFDLDANPAIIEAHLGNDDILKPLIANHPGLRIPGTLDIFELGLRAIL
GQQITVKAATTLFGRFVATFGKPVDTPFPGLDRTSPPAELIADTSLQALIDIGLTGRRALTIQRFAQTIVNGALKPESID
RNKIIEQLLELPGIGPWTAQYIAIRALGDSNAFPASDLGLLRGLRMEKPAELLRRTEKWQPWRAYGAIHLWHQCAGE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=279, Percent_Identity=32.9749103942652, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788542, Length=174, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 53797; Mature: 53797

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSVRCYAVLQARDTNSDGLFFVGVSTTRIYCRPVCRARLPQVDWCTFYSSAARAEQDGY
CCCCEEEEEEEECCCCCCCEEEEEECCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
RPCLRCRPELAPGNLRTSGIGYEVQSTAERIRMGLLMHNNIETLMDELGLQSYQFHQIVE
CHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
REYGVNATELETTQRLLLAKQLLTDTTLEINDIAHTCGFPSVPHFSDTFTSRYRLNPLSL
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCHHHCCCEECCHHHH
RKKYPVNEETTILLRLSYRPPLAWNALIRFLCSRSNLRLSQIQNGNYLQTVNLDGCQGWV
HCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCCCCE
TAKHDTKRHQIYVQASRSLLPCLIRLQMYLRRLFDLDANPAIIEAHLGNDDILKPLIANH
ECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCC
PGLRIPGTLDIFELGLRAILGQQITVKAATTLFGRFVATFGKPVDTPFPGLDRTSPPAEL
CCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
IADTSLQALIDIGLTGRRALTIQRFAQTIVNGALKPESIDRNKIIEQLLELPGIGPWTAQ
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
YIAIRALGDSNAFPASDLGLLRGLRMEKPAELLRRTEKWQPWRAYGAIHLWHQCAGE
EEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSVRCYAVLQARDTNSDGLFFVGVSTTRIYCRPVCRARLPQVDWCTFYSSAARAEQDGY
CCCCEEEEEEEECCCCCCCEEEEEECCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
RPCLRCRPELAPGNLRTSGIGYEVQSTAERIRMGLLMHNNIETLMDELGLQSYQFHQIVE
CHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
REYGVNATELETTQRLLLAKQLLTDTTLEINDIAHTCGFPSVPHFSDTFTSRYRLNPLSL
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCHHHCCCEECCHHHH
RKKYPVNEETTILLRLSYRPPLAWNALIRFLCSRSNLRLSQIQNGNYLQTVNLDGCQGWV
HCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCCCCE
TAKHDTKRHQIYVQASRSLLPCLIRLQMYLRRLFDLDANPAIIEAHLGNDDILKPLIANH
ECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCC
PGLRIPGTLDIFELGLRAILGQQITVKAATTLFGRFVATFGKPVDTPFPGLDRTSPPAEL
CCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
IADTSLQALIDIGLTGRRALTIQRFAQTIVNGALKPESIDRNKIIEQLLELPGIGPWTAQ
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
YIAIRALGDSNAFPASDLGLLRGLRMEKPAELLRRTEKWQPWRAYGAIHLWHQCAGE
EEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]