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The map label for this gene is cysI

Identifier: 114331182

GI number: 114331182

Start: 1234902

End: 1236623

Strand: Reverse

Name: cysI

Synonym: Neut_1184

Alternate gene names: 114331182

Gene position: 1236623-1234902 (Counterclockwise)

Preceding gene: 114331183

Following gene: 114331180

Centisome position: 46.47

GC content: 48.72

Gene sequence:

>1722_bases
ATGGCAGACACACTCCCTCCCACTGATCGCAGCTGCGATATTTCCCAACCACTTGAGCGCCTAAGCCCGGATGAGGCACT
GAAGGCTGGAAGTGAATACCTGCGTGGCACAATCGCACTTGGACTGCTTGACCGTATTACATCTGCTGTACCAGGCGACG
ATATCAAGCTGATGAAGTTTCACGGTATTTATCAACAAGATGACCGGGATGTTCGTGATGAACGCCGTCGCCAAAAACTG
GAACCCGCTTTTCAGTTCATGATCCGTGTGCGTTTGCCGGGGGGTATCTGCACAACCGAACGCTGGCTGAAGATTTCAGA
GCTTGCCTGTGCGCACGGTAATGAAACGTTACGCATGACCACCAGACAGACTTTCCAGTTTCATTGGGTGCTCAAGCACA
ATATTGTTCCGGTTATTCGTGGTCTTCATGAAGTATTGCTCGATACAGTTGCTGCATGCGGAGATGATTCACGCGGTGTA
ATGGCTACGGTTAATCCTCAATTTCCGGCTCTGCAGGCAGAACTGGCAGCACTGGCAAAAACAGTAAGTGATCATGTCAT
TCCCAAAACCAGGGGCTATCATGAAATCTGGTATGGTGAAGAACGTATTGCTTCATCTGAACCGGAAGAACCATTCTACG
GGCGAACCTATATGCCCCGTAAGTTCAAGATTGGATTTGTGATCCCGCCCAATAATGATATTGATATTTATGCTCAGGAT
CTGGGTTATATCGCAATCGCGGGTAAAGATGGAAAAATAGCGGGATTCAATATAGCAATCGGCGGTGGAATGGGGCGTAC
CGACAAGGTACCGCATACCTATCCGCGCACCGCATCAGTTATTGGCTTCATTACGCCAGACAGACTTATTTCTGTTACCG
AAGCTGTCATGGGAGTTCAGCGCGACTATGGCAATCGCGCTGACCGGTCACGTGCACGCTTTAAATACACAATCGATGAT
AAAGGACTGGACTGGATCAAACTGGCAATTGAAGATCGTGCCGGATTGCTCGAACCCGCCCGCCCTTACCACTTCACATC
CAATGCAGATATCTATGGTTGGGTAGAATCGGATGATGGCTATCATCATTTCACGTTGTTTGTTGAGAACGGCCGGCTCA
ACCGCGATACGCTCGACAAAATTGCACGGATTGCACATGTTCACAAAGGGCACTTTCGCCTGACTCCCAATCAAAATCTC
ATGATTGCCAACGTTGCCACTGCCGACAAACCGAAGATAGATGCATTACTGGTGGGATCGGGACTGATTGCTTTTAATGA
ACACAGCGTGCTGCGTCTGAATTCGATGGCGTGTGTCGCTTTTCCAACCTGTGGCTTGGCAATGGCAGACAGTGAACGTT
ATCTGCCTACTTTGATTACGAAGATTGAAGGAATTCTCACTCGGTACAATCTGCAAGATGAGCCAATTACGCTCAGAATG
ACAGGATGTCCTAACGGGTGCAGCCGCCCTTTCATTGCGGAAATTGGACTTACCGGCCGCGCACCGGGTAAATACAATCT
TTATCTGGGTGGCGGTTTTCACGGCCAGCGCCTCAATCGCCTTTATCGTGAAAATATCGGCGAGGCCGCAATTCTTGAAG
CGCTTGATGAAGTACTTCGTCACTATGCGACAGAACGATTGCCTGACGAGCATTTTGGTGATTTTACGATTCGCGCAGGA
ATTATTCGTGAAGTAACAGAAGGACGTTTTTCAAATGACTAA

Upstream 100 bases:

>100_bases
TCGAAGAACAAGCAAGCGTTCAGCGCGAAGAAGCAGAAGAGTATCTTCGCAACCTGCAACGCGATGGGCGCTATCAGCGC
GACGTTTATTGAGAATAGAA

Downstream 100 bases:

>100_bases
CTGATGCTATGCGGCGATACTCTACATATTGCGTAAAAATCTCACAAAAGTGAGGTTATGCGTACAGCAGTGACTCAGAA
GAAGCTACTCTTGCTGTATA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MADTLPPTDRSCDISQPLERLSPDEALKAGSEYLRGTIALGLLDRITSAVPGDDIKLMKFHGIYQQDDRDVRDERRRQKL
EPAFQFMIRVRLPGGICTTERWLKISELACAHGNETLRMTTRQTFQFHWVLKHNIVPVIRGLHEVLLDTVAACGDDSRGV
MATVNPQFPALQAELAALAKTVSDHVIPKTRGYHEIWYGEERIASSEPEEPFYGRTYMPRKFKIGFVIPPNNDIDIYAQD
LGYIAIAGKDGKIAGFNIAIGGGMGRTDKVPHTYPRTASVIGFITPDRLISVTEAVMGVQRDYGNRADRSRARFKYTIDD
KGLDWIKLAIEDRAGLLEPARPYHFTSNADIYGWVESDDGYHHFTLFVENGRLNRDTLDKIARIAHVHKGHFRLTPNQNL
MIANVATADKPKIDALLVGSGLIAFNEHSVLRLNSMACVAFPTCGLAMADSERYLPTLITKIEGILTRYNLQDEPITLRM
TGCPNGCSRPFIAEIGLTGRAPGKYNLYLGGGFHGQRLNRLYRENIGEAAILEALDEVLRHYATERLPDEHFGDFTIRAG
IIREVTEGRFSND

Sequences:

>Translated_573_residues
MADTLPPTDRSCDISQPLERLSPDEALKAGSEYLRGTIALGLLDRITSAVPGDDIKLMKFHGIYQQDDRDVRDERRRQKL
EPAFQFMIRVRLPGGICTTERWLKISELACAHGNETLRMTTRQTFQFHWVLKHNIVPVIRGLHEVLLDTVAACGDDSRGV
MATVNPQFPALQAELAALAKTVSDHVIPKTRGYHEIWYGEERIASSEPEEPFYGRTYMPRKFKIGFVIPPNNDIDIYAQD
LGYIAIAGKDGKIAGFNIAIGGGMGRTDKVPHTYPRTASVIGFITPDRLISVTEAVMGVQRDYGNRADRSRARFKYTIDD
KGLDWIKLAIEDRAGLLEPARPYHFTSNADIYGWVESDDGYHHFTLFVENGRLNRDTLDKIARIAHVHKGHFRLTPNQNL
MIANVATADKPKIDALLVGSGLIAFNEHSVLRLNSMACVAFPTCGLAMADSERYLPTLITKIEGILTRYNLQDEPITLRM
TGCPNGCSRPFIAEIGLTGRAPGKYNLYLGGGFHGQRLNRLYRENIGEAAILEALDEVLRHYATERLPDEHFGDFTIRAG
IIREVTEGRFSND
>Mature_572_residues
ADTLPPTDRSCDISQPLERLSPDEALKAGSEYLRGTIALGLLDRITSAVPGDDIKLMKFHGIYQQDDRDVRDERRRQKLE
PAFQFMIRVRLPGGICTTERWLKISELACAHGNETLRMTTRQTFQFHWVLKHNIVPVIRGLHEVLLDTVAACGDDSRGVM
ATVNPQFPALQAELAALAKTVSDHVIPKTRGYHEIWYGEERIASSEPEEPFYGRTYMPRKFKIGFVIPPNNDIDIYAQDL
GYIAIAGKDGKIAGFNIAIGGGMGRTDKVPHTYPRTASVIGFITPDRLISVTEAVMGVQRDYGNRADRSRARFKYTIDDK
GLDWIKLAIEDRAGLLEPARPYHFTSNADIYGWVESDDGYHHFTLFVENGRLNRDTLDKIARIAHVHKGHFRLTPNQNLM
IANVATADKPKIDALLVGSGLIAFNEHSVLRLNSMACVAFPTCGLAMADSERYLPTLITKIEGILTRYNLQDEPITLRMT
GCPNGCSRPFIAEIGLTGRAPGKYNLYLGGGFHGQRLNRLYRENIGEAAILEALDEVLRHYATERLPDEHFGDFTIRAGI
IREVTEGRFSND

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=552, Percent_Identity=48.5507246376812, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322597, Length=585, Percent_Identity=43.7606837606838, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_NITEC (Q0AGU3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747404.1
- STRING:   Q0AGU3
- GeneID:   4273888
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1184
- NMPDR:   fig|335283.3.peg.933
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   FIAISEN
- PhylomeDB:   Q0AGU3
- ProtClustDB:   PRK13504
- BioCyc:   NEUT335283:NEUT_1184-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 64209; Mature: 64078

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLPPTDRSCDISQPLERLSPDEALKAGSEYLRGTIALGLLDRITSAVPGDDIKLMKF
CCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HGIYQQDDRDVRDERRRQKLEPAFQFMIRVRLPGGICTTERWLKISELACAHGNETLRMT
ECCCCCCCCCHHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEE
TRQTFQFHWVLKHNIVPVIRGLHEVLLDTVAACGDDSRGVMATVNPQFPALQAELAALAK
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
TVSDHVIPKTRGYHEIWYGEERIASSEPEEPFYGRTYMPRKFKIGFVIPPNNDIDIYAQD
HHHHCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEC
LGYIAIAGKDGKIAGFNIAIGGGMGRTDKVPHTYPRTASVIGFITPDRLISVTEAVMGVQ
CCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RDYGNRADRSRARFKYTIDDKGLDWIKLAIEDRAGLLEPARPYHFTSNADIYGWVESDDG
HHCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCC
YHHFTLFVENGRLNRDTLDKIARIAHVHKGHFRLTPNQNLMIANVATADKPKIDALLVGS
EEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCEEEEEC
GLIAFNEHSVLRLNSMACVAFPTCGLAMADSERYLPTLITKIEGILTRYNLQDEPITLRM
CEEEECCCCEEEECCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
TGCPNGCSRPFIAEIGLTGRAPGKYNLYLGGGFHGQRLNRLYRENIGEAAILEALDEVLR
CCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
HYATERLPDEHFGDFTIRAGIIREVTEGRFSND
HHHHHCCCCHHCCCEEEEHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADTLPPTDRSCDISQPLERLSPDEALKAGSEYLRGTIALGLLDRITSAVPGDDIKLMKF
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HGIYQQDDRDVRDERRRQKLEPAFQFMIRVRLPGGICTTERWLKISELACAHGNETLRMT
ECCCCCCCCCHHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEE
TRQTFQFHWVLKHNIVPVIRGLHEVLLDTVAACGDDSRGVMATVNPQFPALQAELAALAK
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
TVSDHVIPKTRGYHEIWYGEERIASSEPEEPFYGRTYMPRKFKIGFVIPPNNDIDIYAQD
HHHHCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEC
LGYIAIAGKDGKIAGFNIAIGGGMGRTDKVPHTYPRTASVIGFITPDRLISVTEAVMGVQ
CCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RDYGNRADRSRARFKYTIDDKGLDWIKLAIEDRAGLLEPARPYHFTSNADIYGWVESDDG
HHCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCC
YHHFTLFVENGRLNRDTLDKIARIAHVHKGHFRLTPNQNLMIANVATADKPKIDALLVGS
EEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCEEEEEC
GLIAFNEHSVLRLNSMACVAFPTCGLAMADSERYLPTLITKIEGILTRYNLQDEPITLRM
CEEEECCCCEEEECCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
TGCPNGCSRPFIAEIGLTGRAPGKYNLYLGGGFHGQRLNRLYRENIGEAAILEALDEVLR
CCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
HYATERLPDEHFGDFTIRAGIIREVTEGRFSND
HHHHHCCCCHHCCCEEEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA