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The map label for this gene is truA

Identifier: 114331148

GI number: 114331148

Start: 1190467

End: 1191300

Strand: Direct

Name: truA

Synonym: Neut_1150

Alternate gene names: 114331148

Gene position: 1190467-1191300 (Clockwise)

Preceding gene: 114331147

Following gene: 114331149

Centisome position: 44.74

GC content: 44.96

Gene sequence:

>834_bases
GTGAAGATCGTCCTGATTCTGGAATATGATGGCCGTGGCTATTGTGGCTGGCAAAAGCAACCTGATCGCATTTCTGTTCA
ATCCCGGCTGGAAAGCGCTTTATCGCGCATAGCAAGCAGTCAGATACAAGTTGTTGCGGCAGGACGAACAGATGCAGGTG
TGCATGCATTGTGTCAGGTCGTACATTTTGAAACGCATGTCATCCGCCCTTTAACAGCTTGGGTAAGAGGAGCTAATGCA
CTACTGCCGGATGATATTTCAGTATTGTGGGCTTCGGAAGTTAATGATGATTTTCATGCCCGATTCAGCGCAACCGAACG
TACTTATCTGTATTATCTGTTGAGTCGGCCTGCCCGCCCTGGAATTTTCCAGGGAAAAACAGGATGGACCCACTATTCGC
TTGATCTCGAAAAAATGCAGACGGCAGCAAGATTCCTGATTGGTGAGCATGATTTCAGTGCATTTCGCTCAGCAGAATGT
CAGGCAAAAAATGCGATACGAAAGCTTGTCAGGCTGGATATTAGTCAAAGTGAACAATTTTTTGTTTTTGAATTTTGTGC
TAATGCGTTCCTGCATCATATGGTTCGAAATATTCTTGGGGCATTGATTTATATTGGCCAGGGAAAGTATCCCCCTGAAT
GGATACAGATACTTCTGGGGAAGCGTGACCGCACACTTGCTGCACCAACATTTTCACCGGATGGATTGTATCTGGCTGGC
GTTCGATACGATGCCAGATGGAATTTGCCTACTTTTAACGTAATCCGGCCAGAGAATGTTATAAATATGATTGCAAGCTT
TAAATATTCACAGAATTGGGCTGGTTTGCGTTAA

Upstream 100 bases:

>100_bases
TATAGCCTACCGGAAGCTATCCTGATTGAGTGCTTTCTTGTATTCCCATATTTGTTGTGAAATTATTCATGAAGGATAAA
GTATTCCTTTATGAAGATCT

Downstream 100 bases:

>100_bases
AAGGAGATCTGGTGCGTATTCGTGTAAAAATATGCGGCATTACCCGGTTAGAAGATGCAATGACGGCTGTACACCATGGG
GTGGATGCTATCGGCTTTAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKIVLILEYDGRGYCGWQKQPDRISVQSRLESALSRIASSQIQVVAAGRTDAGVHALCQVVHFETHVIRPLTAWVRGANA
LLPDDISVLWASEVNDDFHARFSATERTYLYYLLSRPARPGIFQGKTGWTHYSLDLEKMQTAARFLIGEHDFSAFRSAEC
QAKNAIRKLVRLDISQSEQFFVFEFCANAFLHHMVRNILGALIYIGQGKYPPEWIQILLGKRDRTLAAPTFSPDGLYLAG
VRYDARWNLPTFNVIRPENVINMIASFKYSQNWAGLR

Sequences:

>Translated_277_residues
MKIVLILEYDGRGYCGWQKQPDRISVQSRLESALSRIASSQIQVVAAGRTDAGVHALCQVVHFETHVIRPLTAWVRGANA
LLPDDISVLWASEVNDDFHARFSATERTYLYYLLSRPARPGIFQGKTGWTHYSLDLEKMQTAARFLIGEHDFSAFRSAEC
QAKNAIRKLVRLDISQSEQFFVFEFCANAFLHHMVRNILGALIYIGQGKYPPEWIQILLGKRDRTLAAPTFSPDGLYLAG
VRYDARWNLPTFNVIRPENVINMIASFKYSQNWAGLR
>Mature_277_residues
MKIVLILEYDGRGYCGWQKQPDRISVQSRLESALSRIASSQIQVVAAGRTDAGVHALCQVVHFETHVIRPLTAWVRGANA
LLPDDISVLWASEVNDDFHARFSATERTYLYYLLSRPARPGIFQGKTGWTHYSLDLEKMQTAARFLIGEHDFSAFRSAEC
QAKNAIRKLVRLDISQSEQFFVFEFCANAFLHHMVRNILGALIYIGQGKYPPEWIQILLGKRDRTLAAPTFSPDGLYLAG
VRYDARWNLPTFNVIRPENVINMIASFKYSQNWAGLR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=250, Percent_Identity=33.2, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI199559785, Length=258, Percent_Identity=27.1317829457364, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=48.605577689243, Blast_Score=243, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321112, Length=202, Percent_Identity=32.6732673267327, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922728, Length=274, Percent_Identity=26.6423357664234, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_NITEC (Q0AGX7)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747370.1
- ProteinModelPortal:   Q0AGX7
- SMR:   Q0AGX7
- STRING:   Q0AGX7
- GeneID:   4273553
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1150
- NMPDR:   fig|335283.3.peg.967
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q0AGX7
- ProtClustDB:   PRK00021
- BioCyc:   NEUT335283:NEUT_1150-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31528; Mature: 31528

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLILEYDGRGYCGWQKQPDRISVQSRLESALSRIASSQIQVVAAGRTDAGVHALCQV
CEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
VHFETHVIRPLTAWVRGANALLPDDISVLWASEVNDDFHARFSATERTYLYYLLSRPARP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHEEECCCCCEEEEEEECCCCCC
GIFQGKTGWTHYSLDLEKMQTAARFLIGEHDFSAFRSAECQAKNAIRKLVRLDISQSEQF
CEECCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
FVFEFCANAFLHHMVRNILGALIYIGQGKYPPEWIQILLGKRDRTLAAPTFSPDGLYLAG
EHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEE
VRYDARWNLPTFNVIRPENVINMIASFKYSQNWAGLR
EEECCEECCCEEEEECHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIVLILEYDGRGYCGWQKQPDRISVQSRLESALSRIASSQIQVVAAGRTDAGVHALCQV
CEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
VHFETHVIRPLTAWVRGANALLPDDISVLWASEVNDDFHARFSATERTYLYYLLSRPARP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHEEECCCCCEEEEEEECCCCCC
GIFQGKTGWTHYSLDLEKMQTAARFLIGEHDFSAFRSAECQAKNAIRKLVRLDISQSEQF
CEECCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
FVFEFCANAFLHHMVRNILGALIYIGQGKYPPEWIQILLGKRDRTLAAPTFSPDGLYLAG
EHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEE
VRYDARWNLPTFNVIRPENVINMIASFKYSQNWAGLR
EEECCEECCCEEEEECHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA