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The map label for this gene is dfrA [H]

Identifier: 114331020

GI number: 114331020

Start: 1054108

End: 1054638

Strand: Reverse

Name: dfrA [H]

Synonym: Neut_1020

Alternate gene names: 114331020

Gene position: 1054638-1054108 (Counterclockwise)

Preceding gene: 114331021

Following gene: 114331018

Centisome position: 39.63

GC content: 48.4

Gene sequence:

>531_bases
ATGACTATACTGCAACCTGCCACCTCCCGGCTTGCCATTCTAGCTGCCATATCAGCCAACCGAGTGATCGGATTGAATAA
TTCATTACCTTGGCACTTACCAGCAGATCTGAAGCATTTTAAACAGCTAACCACCGGTCATATCGTTGTGATGGGGCGTC
GTACATTTGATTCCATTGGCAAACCTCTGCCTGACCGCACCAATGTTGTGCTCTCTCGTCAGCATGTTCTTGATGTACCA
GGAATTATCACGGCACACTCGATTCAGGAAGTGCTGGATCACTTTGCCAATGATGACAGGCAGATTTTTGTCATTGGAGG
TGCTGAGCTATACCGACAAACACTACCACTGTGTCAGCGCCTGTACCTGACCGAGATTCAGGAGGATTTTGAAGGCGATA
CTTTTTTCCCGGAGTACGATCACGATGACTGGCAGGAAATTTCTCGCGAAATCCACCATGAAACAGGAGGTAGTGGCCTG
GAATACCACTTTGTGATGCTGGATCACAAGCTGCCAGATTCTGGTGGCTGA

Upstream 100 bases:

>100_bases
AGCTGAATCCGGCAATCAGAAATATTCTTGATTTCAGGTACGAGGATTTCACCTTACATAATTATGATCCACATCCGCCC
ATCAAGGCGCCTGTTGCCGT

Downstream 100 bases:

>100_bases
ACAACATAACCTGGCCTACCTGCTGAAGAACTCTACTGCGCTTGCTCCTCACCCCCGGAAACGAGTGATTCAGCTTCTAT
TCGAGCACGCCACTTTTTCA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MTILQPATSRLAILAAISANRVIGLNNSLPWHLPADLKHFKQLTTGHIVVMGRRTFDSIGKPLPDRTNVVLSRQHVLDVP
GIITAHSIQEVLDHFANDDRQIFVIGGAELYRQTLPLCQRLYLTEIQEDFEGDTFFPEYDHDDWQEISREIHHETGGSGL
EYHFVMLDHKLPDSGG

Sequences:

>Translated_176_residues
MTILQPATSRLAILAAISANRVIGLNNSLPWHLPADLKHFKQLTTGHIVVMGRRTFDSIGKPLPDRTNVVLSRQHVLDVP
GIITAHSIQEVLDHFANDDRQIFVIGGAELYRQTLPLCQRLYLTEIQEDFEGDTFFPEYDHDDWQEISREIHHETGGSGL
EYHFVMLDHKLPDSGG
>Mature_175_residues
TILQPATSRLAILAAISANRVIGLNNSLPWHLPADLKHFKQLTTGHIVVMGRRTFDSIGKPLPDRTNVVLSRQHVLDVPG
IITAHSIQEVLDHFANDDRQIFVIGGAELYRQTLPLCQRLYLTEIQEDFEGDTFFPEYDHDDWQEISREIHHETGGSGLE
YHFVMLDHKLPDSGG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=157, Percent_Identity=33.1210191082803, Blast_Score=81, Evalue=5e-16,
Organism=Homo sapiens, GI28827793, Length=154, Percent_Identity=33.1168831168831, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=41.875, Blast_Score=137, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=30.8823529411765, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116008026, Length=183, Percent_Identity=31.1475409836066, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647458, Length=183, Percent_Identity=31.1475409836066, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19917; Mature: 19786

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILQPATSRLAILAAISANRVIGLNNSLPWHLPADLKHFKQLTTGHIVVMGRRTFDSIG
CCCCCCCHHHEEHEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHC
KPLPDRTNVVLSRQHVLDVPGIITAHSIQEVLDHFANDDRQIFVIGGAELYRQTLPLCQR
CCCCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LYLTEIQEDFEGDTFFPEYDHDDWQEISREIHHETGGSGLEYHFVMLDHKLPDSGG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
TILQPATSRLAILAAISANRVIGLNNSLPWHLPADLKHFKQLTTGHIVVMGRRTFDSIG
CCCCCCHHHEEHEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHC
KPLPDRTNVVLSRQHVLDVPGIITAHSIQEVLDHFANDDRQIFVIGGAELYRQTLPLCQR
CCCCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LYLTEIQEDFEGDTFFPEYDHDDWQEISREIHHETGGSGLEYHFVMLDHKLPDSGG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]