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The map label for this gene is thyA

Identifier: 114331021

GI number: 114331021

Start: 1054635

End: 1055429

Strand: Reverse

Name: thyA

Synonym: Neut_1021

Alternate gene names: 114331021

Gene position: 1055429-1054635 (Counterclockwise)

Preceding gene: 114331024

Following gene: 114331020

Centisome position: 39.66

GC content: 47.04

Gene sequence:

>795_bases
ATGCATCCATACCTTGATCTAATGCGACATGTGTTGCAATACGGTCATAAAAAAACTGATCGCACCGGTACCGGGACACT
TTCCATCTTCGGTTATCAGATGCGTTTTGATCTGCAACAGGGGTTCCCGTTGGTAACAACCAAGCAATGCCATGTCAAAT
CGATCATCCACGAATTGCTGTGGTTTCTGCGCGGCGATACCAATATTGACTATCTGAAGAAAAATGGTATTTCCATCTGG
AATGAATGGGCAGATAAAAGCGGGGAGCTGGGACCGGTTTATGGCCACCAGTGGCGTTCCTGGACTGTTTCCGGTGACAA
ATCCATCGATCAGATTGCGCAAGTAATCCAGCAAATCAAAGCAACACCGGATTCACGCCGGATGATTATTTCTGCTTGGA
ACGTGGGAGATCTCGACAAAATGGCACTCGCTCCCTGCCATGTCCTGTTTCAGTTTTATGTGGTGGATGGCAAGCTCTCC
TGCCAACTTTATCAGCGCAGCGCGGATATTTTCCTGGGTGTTCCATTCAACATTGCTTCCTATTCGTTGCTGACACTAAT
GATCGCGCAGTGCTGCAATCTCAAGCCTGGTGAATTCATCCATACATTTGGAGATGCACACCTGTATCTGAATCACCTTG
AACAAGCTCGCCTGCAACTGACTCGTGAACCAAAATCCCTTCCCATAATGGAGCTGAATCCGGCAATCAGAAATATTCTT
GATTTCAGGTACGAGGATTTCACCTTACATAATTATGATCCACATCCGCCCATCAAGGCGCCTGTTGCCGTATGA

Upstream 100 bases:

>100_bases
TTATTTTTTTAAGTAAACTGGCTTTAAAAAAGCCACCTGTACTATTCAATTGATATAGCGCTCTATTATATCTGCTTGAT
TCATACTTTCAGGATTATTC

Downstream 100 bases:

>100_bases
CTATACTGCAACCTGCCACCTCCCGGCTTGCCATTCTAGCTGCCATATCAGCCAACCGAGTGATCGGATTGAATAATTCA
TTACCTTGGCACTTACCAGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHPYLDLMRHVLQYGHKKTDRTGTGTLSIFGYQMRFDLQQGFPLVTTKQCHVKSIIHELLWFLRGDTNIDYLKKNGISIW
NEWADKSGELGPVYGHQWRSWTVSGDKSIDQIAQVIQQIKATPDSRRMIISAWNVGDLDKMALAPCHVLFQFYVVDGKLS
CQLYQRSADIFLGVPFNIASYSLLTLMIAQCCNLKPGEFIHTFGDAHLYLNHLEQARLQLTREPKSLPIMELNPAIRNIL
DFRYEDFTLHNYDPHPPIKAPVAV

Sequences:

>Translated_264_residues
MHPYLDLMRHVLQYGHKKTDRTGTGTLSIFGYQMRFDLQQGFPLVTTKQCHVKSIIHELLWFLRGDTNIDYLKKNGISIW
NEWADKSGELGPVYGHQWRSWTVSGDKSIDQIAQVIQQIKATPDSRRMIISAWNVGDLDKMALAPCHVLFQFYVVDGKLS
CQLYQRSADIFLGVPFNIASYSLLTLMIAQCCNLKPGEFIHTFGDAHLYLNHLEQARLQLTREPKSLPIMELNPAIRNIL
DFRYEDFTLHNYDPHPPIKAPVAV
>Mature_264_residues
MHPYLDLMRHVLQYGHKKTDRTGTGTLSIFGYQMRFDLQQGFPLVTTKQCHVKSIIHELLWFLRGDTNIDYLKKNGISIW
NEWADKSGELGPVYGHQWRSWTVSGDKSIDQIAQVIQQIKATPDSRRMIISAWNVGDLDKMALAPCHVLFQFYVVDGKLS
CQLYQRSADIFLGVPFNIASYSLLTLMIAQCCNLKPGEFIHTFGDAHLYLNHLEQARLQLTREPKSLPIMELNPAIRNIL
DFRYEDFTLHNYDPHPPIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=49.8220640569395, Blast_Score=287, Evalue=8e-78,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.1515151515152, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=284, Percent_Identity=46.1267605633803, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=278, Percent_Identity=41.3669064748201, Blast_Score=201, Evalue=8e-53,
Organism=Drosophila melanogaster, GI17137556, Length=286, Percent_Identity=50.6993006993007, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_NITEC (Q0AHA4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747243.1
- ProteinModelPortal:   Q0AHA4
- SMR:   Q0AHA4
- STRING:   Q0AHA4
- GeneID:   4273864
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1021
- NMPDR:   fig|335283.3.peg.257
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q0AHA4
- ProtClustDB:   PRK01827
- BioCyc:   NEUT335283:NEUT_1021-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30373; Mature: 30373

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPYLDLMRHVLQYGHKKTDRTGTGTLSIFGYQMRFDLQQGFPLVTTKQCHVKSIIHELL
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEHHCCCCEEECCHHHHHHHHHHHH
WFLRGDTNIDYLKKNGISIWNEWADKSGELGPVYGHQWRSWTVSGDKSIDQIAQVIQQIK
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
ATPDSRRMIISAWNVGDLDKMALAPCHVLFQFYVVDGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCEEEEEEEECCCCEEEECCCCHHH
YSLLTLMIAQCCNLKPGEFIHTFGDAHLYLNHLEQARLQLTREPKSLPIMELNPAIRNIL
HHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHH
DFRYEDFTLHNYDPHPPIKAPVAV
HCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MHPYLDLMRHVLQYGHKKTDRTGTGTLSIFGYQMRFDLQQGFPLVTTKQCHVKSIIHELL
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEHHCCCCEEECCHHHHHHHHHHHH
WFLRGDTNIDYLKKNGISIWNEWADKSGELGPVYGHQWRSWTVSGDKSIDQIAQVIQQIK
HHHCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
ATPDSRRMIISAWNVGDLDKMALAPCHVLFQFYVVDGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCEEEEEEEECCCCEEEECCCCHHH
YSLLTLMIAQCCNLKPGEFIHTFGDAHLYLNHLEQARLQLTREPKSLPIMELNPAIRNIL
HHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHH
DFRYEDFTLHNYDPHPPIKAPVAV
HCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA