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The map label for this gene is purA

Identifier: 114330970

GI number: 114330970

Start: 997350

End: 998648

Strand: Direct

Name: purA

Synonym: Neut_0967

Alternate gene names: 114330970

Gene position: 997350-998648 (Clockwise)

Preceding gene: 114330969

Following gene: 114330976

Centisome position: 37.48

GC content: 47.88

Gene sequence:

>1299_bases
ATGGTCAGGAATGTTGTAGTCATTGGAACGCAGTGGGGGGATGAGGGTAAGGGCAAGATTGTCGACTGGCTGACCGATCG
GGCTGCAGGCGTTATCCGTTTCCAGGGAGGACACAATGCTGGGCATACGCTGGTAGTAAATGGTGAGAAAACTGTGCTGC
ATTTGATCCCTTCAGGAATATTGCGCAAGGATGTTATTTGTTACATTGGTAACGGTGTGGTTTTGTCTCCTGGTGCATTG
TTGGACGAAATGAGTATGCTGGAGCAGGCTGGTGTGGATGTGTCCGGCAGGCTACGTATCAGCGAGGCTTGTCCGTTAAT
TTTGCCCTATCACATCGCAGTAGATGGCGCTCGTGAGCTGGCAAAAGGGCCGGAAAAGATTGGCACAACTGGCAGGGGGA
TTGGCCCGGCTTACGAGGACAAGATTGCGCGGCGCGCAATACGTCTGCAGGATCTGTTTTACCCAGAACGATTTGCCAGC
AAATTGAAAGAAGTACTCGACTACCATAATTTCTTGCTGAAGAACTATTACCATACAGCAACGGTAGACTACGACCAGAT
ACTGGATGAATGTCTGAAAAAAGCTGAAAGAATTCAGCCAATGGTCGCAGATGTTCCTAAACTGCTGTTTGAAGCCAACA
AAGCTGGAAACAATCTGTTGTTTGAGGGTGCACAGGGAGCTTTGTTGGATATTGACCATGGCACCTATCCTTTTGTTACT
TCCAGTAATTGTATTGCTGGTGCAGCTTCAGTAGGCAGCGGTGTCGGGCCACAGTTGCTGGGTTACGTACTGGGTATTAC
CAAAGCCTACACCACTCGCGTAGGTTCGGGGCCTTTTCCTACTGAGTTGGCAGATACGACTGGTGAATACTTGGCACAGC
GAGGCAATGAATTTGGCTCCACTACCGGGCGCCCGCGCCGTTGTGGCTGGTTTGATGCAGTGGCTACCAGACGTTCAATT
CAGATCAACGGTGTTTCAGGATTATGTATTACCAAGCTTGATGTGCTTGATGGCTTGGAGAAATTAAAGATTTGTACGGG
TTATAAGTCAAAACAGTCAGACAGATTGTATGATGCTTTGCCATTTGGTGCGGATGATCTGGCTGATATTGAACCAGTTT
ATGAGGAATTGCCAGGATGGCAGGCATGTACGGCGGGAATCAAGGATTTTGATCAACTTCCCAAGACCGCACAAAATTAC
CTCAAACGTATAGAAGAAGTCTGCCAGACACCCATATCGATGATTTCAACCGGTCCTGATCGAATAGAAACAATCGTTTT
TCATCATCCTTTCGGATAA

Upstream 100 bases:

>100_bases
TAGAGGGCTCCGCGCTGTTGGAAGAATATGTCTAAAGCACCACGCTGTAAACAGCGTGGTGCTTTATTTTGGAAATAATT
TCTGAGAGATCGATGAGTGT

Downstream 100 bases:

>100_bases
ATGCAGCGTAATCTGAGCTGGTGGGCACTGCCTGACAATCTCCCCGTATGGCCTTATTCCCTAATTCCTATTGATGCCAT
TTCTGGTAAAGCTCTTCGGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVRNVVVIGTQWGDEGKGKIVDWLTDRAAGVIRFQGGHNAGHTLVVNGEKTVLHLIPSGILRKDVICYIGNGVVLSPGAL
LDEMSMLEQAGVDVSGRLRISEACPLILPYHIAVDGARELAKGPEKIGTTGRGIGPAYEDKIARRAIRLQDLFYPERFAS
KLKEVLDYHNFLLKNYYHTATVDYDQILDECLKKAERIQPMVADVPKLLFEANKAGNNLLFEGAQGALLDIDHGTYPFVT
SSNCIAGAASVGSGVGPQLLGYVLGITKAYTTRVGSGPFPTELADTTGEYLAQRGNEFGSTTGRPRRCGWFDAVATRRSI
QINGVSGLCITKLDVLDGLEKLKICTGYKSKQSDRLYDALPFGADDLADIEPVYEELPGWQACTAGIKDFDQLPKTAQNY
LKRIEEVCQTPISMISTGPDRIETIVFHHPFG

Sequences:

>Translated_432_residues
MVRNVVVIGTQWGDEGKGKIVDWLTDRAAGVIRFQGGHNAGHTLVVNGEKTVLHLIPSGILRKDVICYIGNGVVLSPGAL
LDEMSMLEQAGVDVSGRLRISEACPLILPYHIAVDGARELAKGPEKIGTTGRGIGPAYEDKIARRAIRLQDLFYPERFAS
KLKEVLDYHNFLLKNYYHTATVDYDQILDECLKKAERIQPMVADVPKLLFEANKAGNNLLFEGAQGALLDIDHGTYPFVT
SSNCIAGAASVGSGVGPQLLGYVLGITKAYTTRVGSGPFPTELADTTGEYLAQRGNEFGSTTGRPRRCGWFDAVATRRSI
QINGVSGLCITKLDVLDGLEKLKICTGYKSKQSDRLYDALPFGADDLADIEPVYEELPGWQACTAGIKDFDQLPKTAQNY
LKRIEEVCQTPISMISTGPDRIETIVFHHPFG
>Mature_432_residues
MVRNVVVIGTQWGDEGKGKIVDWLTDRAAGVIRFQGGHNAGHTLVVNGEKTVLHLIPSGILRKDVICYIGNGVVLSPGAL
LDEMSMLEQAGVDVSGRLRISEACPLILPYHIAVDGARELAKGPEKIGTTGRGIGPAYEDKIARRAIRLQDLFYPERFAS
KLKEVLDYHNFLLKNYYHTATVDYDQILDECLKKAERIQPMVADVPKLLFEANKAGNNLLFEGAQGALLDIDHGTYPFVT
SSNCIAGAASVGSGVGPQLLGYVLGITKAYTTRVGSGPFPTELADTTGEYLAQRGNEFGSTTGRPRRCGWFDAVATRRSI
QINGVSGLCITKLDVLDGLEKLKICTGYKSKQSDRLYDALPFGADDLADIEPVYEELPGWQACTAGIKDFDQLPKTAQNY
LKRIEEVCQTPISMISTGPDRIETIVFHHPFG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=43.1235431235431, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=42.4593967517401, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=41.8136020151133, Blast_Score=292, Evalue=4e-79,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=61.9489559164733, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=42.3255813953488, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=42.3255813953488, Blast_Score=325, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=40.7494145199063, Blast_Score=308, Evalue=9e-85,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.1176470588235, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NITEC (Q0AHF5)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747192.1
- ProteinModelPortal:   Q0AHF5
- SMR:   Q0AHF5
- STRING:   Q0AHF5
- GeneID:   4273539
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0967
- NMPDR:   fig|335283.3.peg.1555
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q0AHF5
- ProtClustDB:   PRK01117
- BioCyc:   NEUT335283:NEUT_0967-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47088; Mature: 47088

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRNVVVIGTQWGDEGKGKIVDWLTDRAAGVIRFQGGHNAGHTLVVNGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRKDVICYIGNGVVLSPGALLDEMSMLEQAGVDVSGRLRISEACPLILPYHIAVDGAREL
HHHCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEEEEEECCHHHH
AKGPEKIGTTGRGIGPAYEDKIARRAIRLQDLFYPERFASKLKEVLDYHNFLLKNYYHTA
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHE
TVDYDQILDECLKKAERIQPMVADVPKLLFEANKAGNNLLFEGAQGALLDIDHGTYPFVT
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCEE
SSNCIAGAASVGSGVGPQLLGYVLGITKAYTTRVGSGPFPTELADTTGEYLAQRGNEFGS
CCCCHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTGRPRRCGWFDAVATRRSIQINGVSGLCITKLDVLDGLEKLKICTGYKSKQSDRLYDAL
CCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
PFGADDLADIEPVYEELPGWQACTAGIKDFDQLPKTAQNYLKRIEEVCQTPISMISTGPD
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHCCCHH
RIETIVFHHPFG
HEEEEEEECCCC
>Mature Secondary Structure
MVRNVVVIGTQWGDEGKGKIVDWLTDRAAGVIRFQGGHNAGHTLVVNGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRKDVICYIGNGVVLSPGALLDEMSMLEQAGVDVSGRLRISEACPLILPYHIAVDGAREL
HHHCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEEEEEECCHHHH
AKGPEKIGTTGRGIGPAYEDKIARRAIRLQDLFYPERFASKLKEVLDYHNFLLKNYYHTA
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHE
TVDYDQILDECLKKAERIQPMVADVPKLLFEANKAGNNLLFEGAQGALLDIDHGTYPFVT
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCEE
SSNCIAGAASVGSGVGPQLLGYVLGITKAYTTRVGSGPFPTELADTTGEYLAQRGNEFGS
CCCCHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCC
TTGRPRRCGWFDAVATRRSIQINGVSGLCITKLDVLDGLEKLKICTGYKSKQSDRLYDAL
CCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
PFGADDLADIEPVYEELPGWQACTAGIKDFDQLPKTAQNYLKRIEEVCQTPISMISTGPD
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHCCCHH
RIETIVFHHPFG
HEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA