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The map label for this gene is hisZ

Identifier: 114330969

GI number: 114330969

Start: 996042

End: 997214

Strand: Direct

Name: hisZ

Synonym: Neut_0966

Alternate gene names: 114330969

Gene position: 996042-997214 (Clockwise)

Preceding gene: 114330968

Following gene: 114330970

Centisome position: 37.43

GC content: 48.42

Gene sequence:

>1173_bases
ATGCGTAACTGGTTGCTTCCTGAATATATCGAAGATGTACTGCCGCGTGACGCTTATCGTATAGAAAAGATTCGTCGTCT
TGTCATGGACAAGCTGTTTGTTCATGGATATCAGTTTGTGATGCCACCCCTGTTGGAATATGTGGAGTCTCTGCTGGCCG
GGAGTGGCAGTGGCATGAATCTGCGTATGTTCAAGGTTGTCGATCAACTGAGCGGCAGGATGATGGGGTTACGGGCAGAT
ATGACACCGCAGGCAGCCCGGATTGATGCACATTTGCTGAACACCAATGGTGTGACTCGGCTTTGTTATGCCAGCAGTGT
TGTTCATACGGTACCTGATGAAATTACCCGCACACGAGAGCCATTTCAGGTGGGGGCCGAATTATATGGGCATTCCGGTA
TCGAGAGTGATCTTGAAATCCAGCGTTTGCTATTGGAATGCTTGTCTGTTTCCGGTATCCAGTCGATTCATCTTGATCTT
GGACATATCAGAGTGTTCCGCAGTCTGATTTATGGTTCAGGAATCAGGCCCGAGTTTGAAATGGAGCTTTATGCTGCATT
GTGGGCCAAGGATACGAGTGCTCTTAGGGAGCTGGTGTACACTGGCCTGAATGACTTGGATAAGACGGTTCGGGAAGCAT
TGCTGCTGTTGCCGGAGTTGTACGGAGATGAAACTGTTCTTTTACGCGCACGCAAATACCTACCGAATTTTCCGGAGATC
AGGGAGGCTCTGGATCAACTGGAGCATGTTGCGAAGGCACTTGAACCTTATGTTGACAGGATAATTTTTGATCTGGCCGA
CTTGCGAGGTTATCACTATCACACGGGGATGGTGTTTGCCGCATATACACAGGGAAGCCCTGTCGCGATTGCATTGGGAG
GGCGCTATGATGAAATCGGTAAATCATTCGGTAGAGCGAGACCAGCAACCGGATTCAGCCTTGATTTGAAACAATTATCA
CGTCTGACTGATATAAATGATTATCCCCGAGGAATACTGGCACCGTGGAAGCCGCAGGATGATGAACTTGCAGCAATGAT
CAAGCAGCTGCGTATCGCAGGGCATATCGTGGTGACAGAATTGCCTGGAGAGAACAGTAAAGAAGTAGCCAGGTGTGACA
GAAATCTGGTTTTACGGAATGGCAAATGGGAAATTTGCCCTGTTTCAGGGTAA

Upstream 100 bases:

>100_bases
TTCTTTATCTGGTTAGTTGATTGATTGATATCTTTCCTCTGATAATTTTGACTGTTCCTGTTATTGATCCAGGTTTAATT
CATTCTGGGCAACATTAAAA

Downstream 100 bases:

>100_bases
ACAGACATCTGTAGTAGGGAATCTCTCTTTAAAGATAGAGGGCTCCGCGCTGTTGGAAGAATATGTCTAAAGCACCACGC
TGTAAACAGCGTGGTGCTTT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRNWLLPEYIEDVLPRDAYRIEKIRRLVMDKLFVHGYQFVMPPLLEYVESLLAGSGSGMNLRMFKVVDQLSGRMMGLRAD
MTPQAARIDAHLLNTNGVTRLCYASSVVHTVPDEITRTREPFQVGAELYGHSGIESDLEIQRLLLECLSVSGIQSIHLDL
GHIRVFRSLIYGSGIRPEFEMELYAALWAKDTSALRELVYTGLNDLDKTVREALLLLPELYGDETVLLRARKYLPNFPEI
REALDQLEHVAKALEPYVDRIIFDLADLRGYHYHTGMVFAAYTQGSPVAIALGGRYDEIGKSFGRARPATGFSLDLKQLS
RLTDINDYPRGILAPWKPQDDELAAMIKQLRIAGHIVVTELPGENSKEVARCDRNLVLRNGKWEICPVSG

Sequences:

>Translated_390_residues
MRNWLLPEYIEDVLPRDAYRIEKIRRLVMDKLFVHGYQFVMPPLLEYVESLLAGSGSGMNLRMFKVVDQLSGRMMGLRAD
MTPQAARIDAHLLNTNGVTRLCYASSVVHTVPDEITRTREPFQVGAELYGHSGIESDLEIQRLLLECLSVSGIQSIHLDL
GHIRVFRSLIYGSGIRPEFEMELYAALWAKDTSALRELVYTGLNDLDKTVREALLLLPELYGDETVLLRARKYLPNFPEI
REALDQLEHVAKALEPYVDRIIFDLADLRGYHYHTGMVFAAYTQGSPVAIALGGRYDEIGKSFGRARPATGFSLDLKQLS
RLTDINDYPRGILAPWKPQDDELAAMIKQLRIAGHIVVTELPGENSKEVARCDRNLVLRNGKWEICPVSG
>Mature_390_residues
MRNWLLPEYIEDVLPRDAYRIEKIRRLVMDKLFVHGYQFVMPPLLEYVESLLAGSGSGMNLRMFKVVDQLSGRMMGLRAD
MTPQAARIDAHLLNTNGVTRLCYASSVVHTVPDEITRTREPFQVGAELYGHSGIESDLEIQRLLLECLSVSGIQSIHLDL
GHIRVFRSLIYGSGIRPEFEMELYAALWAKDTSALRELVYTGLNDLDKTVREALLLLPELYGDETVLLRARKYLPNFPEI
REALDQLEHVAKALEPYVDRIIFDLADLRGYHYHTGMVFAAYTQGSPVAIALGGRYDEIGKSFGRARPATGFSLDLKQLS
RLTDINDYPRGILAPWKPQDDELAAMIKQLRIAGHIVVTELPGENSKEVARCDRNLVLRNGKWEICPVSG

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_NITEC (Q0AHF6)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747191.1
- ProteinModelPortal:   Q0AHF6
- STRING:   Q0AHF6
- GeneID:   4273538
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0966
- NMPDR:   fig|335283.3.peg.1554
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- BioCyc:   NEUT335283:NEUT_0966-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44086; Mature: 44086

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNWLLPEYIEDVLPRDAYRIEKIRRLVMDKLFVHGYQFVMPPLLEYVESLLAGSGSGMN
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LRMFKVVDQLSGRMMGLRADMTPQAARIDAHLLNTNGVTRLCYASSVVHTVPDEITRTRE
CHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHCCC
PFQVGAELYGHSGIESDLEIQRLLLECLSVSGIQSIHLDLGHIRVFRSLIYGSGIRPEFE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
MELYAALWAKDTSALRELVYTGLNDLDKTVREALLLLPELYGDETVLLRARKYLPNFPEI
HHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
REALDQLEHVAKALEPYVDRIIFDLADLRGYHYHTGMVFAAYTQGSPVAIALGGRYDEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCEEEEECCCHHHHH
KSFGRARPATGFSLDLKQLSRLTDINDYPRGILAPWKPQDDELAAMIKQLRIAGHIVVTE
HHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
LPGENSKEVARCDRNLVLRNGKWEICPVSG
CCCCCCHHHHHHCCCEEEECCCEEEEECCC
>Mature Secondary Structure
MRNWLLPEYIEDVLPRDAYRIEKIRRLVMDKLFVHGYQFVMPPLLEYVESLLAGSGSGMN
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LRMFKVVDQLSGRMMGLRADMTPQAARIDAHLLNTNGVTRLCYASSVVHTVPDEITRTRE
CHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHCCC
PFQVGAELYGHSGIESDLEIQRLLLECLSVSGIQSIHLDLGHIRVFRSLIYGSGIRPEFE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
MELYAALWAKDTSALRELVYTGLNDLDKTVREALLLLPELYGDETVLLRARKYLPNFPEI
HHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
REALDQLEHVAKALEPYVDRIIFDLADLRGYHYHTGMVFAAYTQGSPVAIALGGRYDEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCEEEEECCCHHHHH
KSFGRARPATGFSLDLKQLSRLTDINDYPRGILAPWKPQDDELAAMIKQLRIAGHIVVTE
HHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
LPGENSKEVARCDRNLVLRNGKWEICPVSG
CCCCCCHHHHHHCCCEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA