| Definition | Nitrosomonas eutropha C91, complete genome. |
|---|---|
| Accession | NC_008344 |
| Length | 2,661,057 |
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The map label for this gene is polA [H]
Identifier: 114330791
GI number: 114330791
Start: 794818
End: 797538
Strand: Direct
Name: polA [H]
Synonym: Neut_0778
Alternate gene names: 114330791
Gene position: 794818-797538 (Clockwise)
Preceding gene: 114330786
Following gene: 114330792
Centisome position: 29.87
GC content: 47.78
Gene sequence:
>2721_bases ATGAAAACACTCGTGCTGGTTGATGGCTCTTCTTACCTGTATCGTGCCTTTCATGCGTTGCCCGATTTACGTAATCGCCT GAATGAACCGACTGGTGCAATTTATGGCGTACTGAATATGCTGCGTCGCTTGTATAAGGAATACCAGCCAGATTATAGCG CGTGTGTGTTTGACGCAAAAGGTAAAACGTTCCGGGAAGAGATTTATCCGCAATATAAAGCAAACCGTCCGCCGATGCCG GCAGATTTGGCTGGCCAGATAAATCCCTTGTATGCCTGTATCCGGGCAATGGGCTGGCCGCTGCTGGTGGAAGAGCAGGT CGAGGCGGATGATGTCATCGGTACGCTGGCCAGACGTGCAGCAGGTGAACGGATACAATGTGTTATATCGACGGGTGACA AGGATATTGCGCAATTGGTTGCGCCAGGTATACGACTGGTAAATACCATGAATAGTGAATCACTGGATGAGGCGGGTGTG CTGCAAAAATTCGGTGTGATGCCTGCGCAAATCATCGATTTTCTGGCGTTGGTGGGGGATAGTGTAGATAACATTCCCGG CGTAGAAAAAGTAGGCCCCAAAACTGCGGTGAAATGGCTTAATCAATATGGCTCGTTGAATAATCTGATCGCACATGCAC ATGAAATAAAGGGTGTGGTGGGTGAAAATTTGCGCAAGGCACTGGATTGGTTACAGGTTTCAAGAGAGTTGCTAACAATC AAGTGCGATGTTCCGCTTGCAATGGGTTGGCAGGATCTGGTTGCTGCTCCTCTTGATGTTGCTCGGTTGATGGCGCTTTA TGAGCATCTTGAATTCCGAAGTTGGTTACGTGAACTCAGGCAATCAGATTCTGAAACAGGCCCAGCAACTATACCTATGT ATGATCCATTAGTGCCGGGAGAAAGAAACAGCGATAGCCGGGATTATCAAATCATCCTGACTGATTCCCAATTTGAAGAA TGGGTCCAGCAATGCGAGTCTGCTGAACTGGTTTCGATTGATACCGAAACAACCAGCCTGAATCCAATGGAGGCTGAACT GGTGGGGCTTTCGTTATGTACGGAGCCGGGGCACGCAGCTTATATACCGCTGGCACATCATTATCAGGGTGTGCCTTCAC AGCTAAATCGTGAACAGGTACTGCAACGGTTGAAGCCCTGGCTGGAGAATAGTGATATACCCAAGGTAGGGCAGAATCTG AAATACGATCGGCATGTGTTTGCTAACCATGGGGTCATGCTGAACGGAATCGCGCACGATACGCTGTTGCAATCGTATGT GCTGGAATCCCATCTGTCACACGATCTGGACAGTCTGGCCCGCCGACATTTGGATATCCAGACCATCAGCTATGATGATG TAACCGGGAAGGGCGCAAAGCGTATCGGGTTTGAACAGGTGGAAATTCAACAAGCGGGGATTTATGCTGCTGAAGATGCT GATATTCCCTTGCGTCTGCATCATTTTTTGTATCCAATCATCAGTAAGGATGCTCATCTTGGACATATTTATCAGCAGAT TGAAATGCCATTGCTGGAGGTTCTGTTCCGGATAGAGCGTAATGGTGTGTTGCTTGATACCGATCTTCTACGGGAGCAAA GTGGTGAACTGGCGCGGCAACTGGTCATACTTGAGCAGCAGGCCCATTCCCTGGCTGGCCGTGCTTTTAATCTGAACTCA ACTAAACAGATACAAGAGATTTTATTTGGGCAGCATAAGTTGCCAGTTATTAAAAAAACACCAAAAGGTGCGCCATCCAC TGATGAAGAAGTTTTACAACAGCTGGCTCTGGATTATCCGTTACCCAAGATCTTGCTGGATTACCGGAGTCTTGCCAAGC TCAGATCGACTTATATCGATAAGCTACCGCAAATGGTGAATAGACAGACCGGTCGTGTCCATACAAATTATGCGCAGGCG GTGGCAGTTACCGGTCGCCTGGCGAGTAACGATCCCAATTTGCAGAATATTCCGGTACGCACACCGGAAGGCAGGCGTAT CCGTGAGGCATTTATTGCTCCGAGTGGCTGGTATATTATTTCAGCAGATTATTCCCAGATTGAGTTGCGTATCATGGCGC ATATTTCCCAGGACGCTGGGTTGATACGGGCATTTTTGGGAGGGCAGGACATTCATAGCGCTACTGCTGCCGAGGTATTT GGCATTTCTGTTGGTCAGGTGAATGCAGAGCAGCGCCGCTACGCTAAAGTCATCAATTTTGGTCTGATTTATGGCATGTC GGAATTTGGGCTGGCCACCCAGCTGGGTATTGATCGTAGCGCTGCGCGAGCGTTTATTGATCGTTATTTTGCGCGTTACC CAGGAGTGGCAGATTACATGCAACGTACAAGAGAGCTTGCGAGACAGAATGGATATGTTGAAACTGTGCTGGGAAGAAGA CTGCAGCTACCTGATATTCGCAGTAGCCAGCATAATCGCCGGATGGGAGCTGAACGGGCGGCGATTAATGCCCCAATGCA GGGTACAGCTGCTGATATTATTAAATTAGCAATGATTTCCGTACATCAATGGCTGGTAAAAGCGCCGCTTCAAAGTAAAT TGATCATGCAGGTACATGATGAATTGGTGTTGGAGGTGATTGAGGATGAACTATTTGTAGTTAAGGAAAATCTAATAATA TTGATGGAAAATACGTTTAAGTTGGATGTGCCATTAAAGGTTGAGGTCGGGGTTGGGAAAAATTGGGATCAGGCGCATTA A
Upstream 100 bases:
>100_bases CAATACCGAAAACCCGCCATGATTCTCTGGCTGACCAGGGTTTTTCCAGGGTAAGGGCATCCCCCAACTTTGCTTGTAAA CTCATAAAGGTCCTTATTTG
Downstream 100 bases:
>100_bases CTTTCTATTTGATCCACCTTCTTTTCGAGAATCTTATGAAATTTCTTGACTAATAAGTAGCATCTATTTAGATTGAGCTG TTATAGAATAATTTATGATT
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 906; Mature: 906
Protein sequence:
>906_residues MKTLVLVDGSSYLYRAFHALPDLRNRLNEPTGAIYGVLNMLRRLYKEYQPDYSACVFDAKGKTFREEIYPQYKANRPPMP ADLAGQINPLYACIRAMGWPLLVEEQVEADDVIGTLARRAAGERIQCVISTGDKDIAQLVAPGIRLVNTMNSESLDEAGV LQKFGVMPAQIIDFLALVGDSVDNIPGVEKVGPKTAVKWLNQYGSLNNLIAHAHEIKGVVGENLRKALDWLQVSRELLTI KCDVPLAMGWQDLVAAPLDVARLMALYEHLEFRSWLRELRQSDSETGPATIPMYDPLVPGERNSDSRDYQIILTDSQFEE WVQQCESAELVSIDTETTSLNPMEAELVGLSLCTEPGHAAYIPLAHHYQGVPSQLNREQVLQRLKPWLENSDIPKVGQNL KYDRHVFANHGVMLNGIAHDTLLQSYVLESHLSHDLDSLARRHLDIQTISYDDVTGKGAKRIGFEQVEIQQAGIYAAEDA DIPLRLHHFLYPIISKDAHLGHIYQQIEMPLLEVLFRIERNGVLLDTDLLREQSGELARQLVILEQQAHSLAGRAFNLNS TKQIQEILFGQHKLPVIKKTPKGAPSTDEEVLQQLALDYPLPKILLDYRSLAKLRSTYIDKLPQMVNRQTGRVHTNYAQA VAVTGRLASNDPNLQNIPVRTPEGRRIREAFIAPSGWYIISADYSQIELRIMAHISQDAGLIRAFLGGQDIHSATAAEVF GISVGQVNAEQRRYAKVINFGLIYGMSEFGLATQLGIDRSAARAFIDRYFARYPGVADYMQRTRELARQNGYVETVLGRR LQLPDIRSSQHNRRMGAERAAINAPMQGTAADIIKLAMISVHQWLVKAPLQSKLIMQVHDELVLEVIEDELFVVKENLII LMENTFKLDVPLKVEVGVGKNWDQAH
Sequences:
>Translated_906_residues MKTLVLVDGSSYLYRAFHALPDLRNRLNEPTGAIYGVLNMLRRLYKEYQPDYSACVFDAKGKTFREEIYPQYKANRPPMP ADLAGQINPLYACIRAMGWPLLVEEQVEADDVIGTLARRAAGERIQCVISTGDKDIAQLVAPGIRLVNTMNSESLDEAGV LQKFGVMPAQIIDFLALVGDSVDNIPGVEKVGPKTAVKWLNQYGSLNNLIAHAHEIKGVVGENLRKALDWLQVSRELLTI KCDVPLAMGWQDLVAAPLDVARLMALYEHLEFRSWLRELRQSDSETGPATIPMYDPLVPGERNSDSRDYQIILTDSQFEE WVQQCESAELVSIDTETTSLNPMEAELVGLSLCTEPGHAAYIPLAHHYQGVPSQLNREQVLQRLKPWLENSDIPKVGQNL KYDRHVFANHGVMLNGIAHDTLLQSYVLESHLSHDLDSLARRHLDIQTISYDDVTGKGAKRIGFEQVEIQQAGIYAAEDA DIPLRLHHFLYPIISKDAHLGHIYQQIEMPLLEVLFRIERNGVLLDTDLLREQSGELARQLVILEQQAHSLAGRAFNLNS TKQIQEILFGQHKLPVIKKTPKGAPSTDEEVLQQLALDYPLPKILLDYRSLAKLRSTYIDKLPQMVNRQTGRVHTNYAQA VAVTGRLASNDPNLQNIPVRTPEGRRIREAFIAPSGWYIISADYSQIELRIMAHISQDAGLIRAFLGGQDIHSATAAEVF GISVGQVNAEQRRYAKVINFGLIYGMSEFGLATQLGIDRSAARAFIDRYFARYPGVADYMQRTRELARQNGYVETVLGRR LQLPDIRSSQHNRRMGAERAAINAPMQGTAADIIKLAMISVHQWLVKAPLQSKLIMQVHDELVLEVIEDELFVVKENLII LMENTFKLDVPLKVEVGVGKNWDQAH >Mature_906_residues MKTLVLVDGSSYLYRAFHALPDLRNRLNEPTGAIYGVLNMLRRLYKEYQPDYSACVFDAKGKTFREEIYPQYKANRPPMP ADLAGQINPLYACIRAMGWPLLVEEQVEADDVIGTLARRAAGERIQCVISTGDKDIAQLVAPGIRLVNTMNSESLDEAGV LQKFGVMPAQIIDFLALVGDSVDNIPGVEKVGPKTAVKWLNQYGSLNNLIAHAHEIKGVVGENLRKALDWLQVSRELLTI KCDVPLAMGWQDLVAAPLDVARLMALYEHLEFRSWLRELRQSDSETGPATIPMYDPLVPGERNSDSRDYQIILTDSQFEE WVQQCESAELVSIDTETTSLNPMEAELVGLSLCTEPGHAAYIPLAHHYQGVPSQLNREQVLQRLKPWLENSDIPKVGQNL KYDRHVFANHGVMLNGIAHDTLLQSYVLESHLSHDLDSLARRHLDIQTISYDDVTGKGAKRIGFEQVEIQQAGIYAAEDA DIPLRLHHFLYPIISKDAHLGHIYQQIEMPLLEVLFRIERNGVLLDTDLLREQSGELARQLVILEQQAHSLAGRAFNLNS TKQIQEILFGQHKLPVIKKTPKGAPSTDEEVLQQLALDYPLPKILLDYRSLAKLRSTYIDKLPQMVNRQTGRVHTNYAQA VAVTGRLASNDPNLQNIPVRTPEGRRIREAFIAPSGWYIISADYSQIELRIMAHISQDAGLIRAFLGGQDIHSATAAEVF GISVGQVNAEQRRYAKVINFGLIYGMSEFGLATQLGIDRSAARAFIDRYFARYPGVADYMQRTRELARQNGYVETVLGRR LQLPDIRSSQHNRRMGAERAAINAPMQGTAADIIKLAMISVHQWLVKAPLQSKLIMQVHDELVLEVIEDELFVVKENLII LMENTFKLDVPLKVEVGVGKNWDQAH
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=439, Percent_Identity=32.1184510250569, Blast_Score=229, Evalue=1e-59, Organism=Homo sapiens, GI139394648, Length=518, Percent_Identity=29.3436293436293, Blast_Score=207, Evalue=3e-53, Organism=Escherichia coli, GI1790294, Length=925, Percent_Identity=54.1621621621622, Blast_Score=947, Evalue=0.0, Organism=Escherichia coli, GI226510970, Length=228, Percent_Identity=31.140350877193, Blast_Score=108, Evalue=1e-24, Organism=Caenorhabditis elegans, GI86563272, Length=422, Percent_Identity=27.4881516587678, Blast_Score=167, Evalue=3e-41, Organism=Drosophila melanogaster, GI17933644, Length=423, Percent_Identity=31.4420803782506, Blast_Score=194, Evalue=2e-49,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 101756; Mature: 101756
Theoretical pI: Translated: 6.20; Mature: 6.20
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKTLVLVDGSSYLYRAFHALPDLRNRLNEPTGAIYGVLNMLRRLYKEYQPDYSACVFDAK CCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCC GKTFREEIYPQYKANRPPMPADLAGQINPLYACIRAMGWPLLVEEQVEADDVIGTLARRA CCHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHC AGERIQCVISTGDKDIAQLVAPGIRLVNTMNSESLDEAGVLQKFGVMPAQIIDFLALVGD CCCEEEEEEECCCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC SVDNIPGVEKVGPKTAVKWLNQYGSLNNLIAHAHEIKGVVGENLRKALDWLQVSRELLTI CCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE KCDVPLAMGWQDLVAAPLDVARLMALYEHLEFRSWLRELRQSDSETGPATIPMYDPLVPG EECCCHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCC ERNSDSRDYQIILTDSQFEEWVQQCESAELVSIDTETTSLNPMEAELVGLSLCTEPGHAA CCCCCCCCEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHEEECCCCCEE YIPLAHHYQGVPSQLNREQVLQRLKPWLENSDIPKVGQNLKYDRHVFANHGVMLNGIAHD EEEHHHHCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCEEEECCHHH TLLQSYVLESHLSHDLDSLARRHLDIQTISYDDVTGKGAKRIGFEQVEIQQAGIYAAEDA HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCHHHHHHHCCEEEECCC DIPLRLHHFLYPIISKDAHLGHIYQQIEMPLLEVLFRIERNGVLLDTDLLREQSGELARQ CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHH LVILEQQAHSLAGRAFNLNSTKQIQEILFGQHKLPVIKKTPKGAPSTDEEVLQQLALDYP HHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCC LPKILLDYRSLAKLRSTYIDKLPQMVNRQTGRVHTNYAQAVAVTGRLASNDPNLQNIPVR CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHEECEECCCCCCCCCCCCC TPEGRRIREAFIAPSGWYIISADYSQIELRIMAHISQDAGLIRAFLGGQDIHSATAAEVF CCCCCHHHHHHCCCCCCEEEECCCCCEEEHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHH GISVGQVNAEQRRYAKVINFGLIYGMSEFGLATQLGIDRSAARAFIDRYFARYPGVADYM CCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHH QRTRELARQNGYVETVLGRRLQLPDIRSSQHNRRMGAERAAINAPMQGTAADIIKLAMIS HHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHH VHQWLVKAPLQSKLIMQVHDELVLEVIEDELFVVKENLIILMENTFKLDVPLKVEVGVGK HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCEEEECCEEEEECCCC NWDQAH CCCCCC >Mature Secondary Structure MKTLVLVDGSSYLYRAFHALPDLRNRLNEPTGAIYGVLNMLRRLYKEYQPDYSACVFDAK CCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCC GKTFREEIYPQYKANRPPMPADLAGQINPLYACIRAMGWPLLVEEQVEADDVIGTLARRA CCHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHC AGERIQCVISTGDKDIAQLVAPGIRLVNTMNSESLDEAGVLQKFGVMPAQIIDFLALVGD CCCEEEEEEECCCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC SVDNIPGVEKVGPKTAVKWLNQYGSLNNLIAHAHEIKGVVGENLRKALDWLQVSRELLTI CCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE KCDVPLAMGWQDLVAAPLDVARLMALYEHLEFRSWLRELRQSDSETGPATIPMYDPLVPG EECCCHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCC ERNSDSRDYQIILTDSQFEEWVQQCESAELVSIDTETTSLNPMEAELVGLSLCTEPGHAA CCCCCCCCEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHEEECCCCCEE YIPLAHHYQGVPSQLNREQVLQRLKPWLENSDIPKVGQNLKYDRHVFANHGVMLNGIAHD EEEHHHHCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCEEEECCHHH TLLQSYVLESHLSHDLDSLARRHLDIQTISYDDVTGKGAKRIGFEQVEIQQAGIYAAEDA HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCHHHHHHHCCEEEECCC DIPLRLHHFLYPIISKDAHLGHIYQQIEMPLLEVLFRIERNGVLLDTDLLREQSGELARQ CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHH LVILEQQAHSLAGRAFNLNSTKQIQEILFGQHKLPVIKKTPKGAPSTDEEVLQQLALDYP HHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCC LPKILLDYRSLAKLRSTYIDKLPQMVNRQTGRVHTNYAQAVAVTGRLASNDPNLQNIPVR CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHEECEECCCCCCCCCCCCC TPEGRRIREAFIAPSGWYIISADYSQIELRIMAHISQDAGLIRAFLGGQDIHSATAAEVF CCCCCHHHHHHCCCCCCEEEECCCCCEEEHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHH GISVGQVNAEQRRYAKVINFGLIYGMSEFGLATQLGIDRSAARAFIDRYFARYPGVADYM CCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHH QRTRELARQNGYVETVLGRRLQLPDIRSSQHNRRMGAERAAINAPMQGTAADIIKLAMIS HHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHH VHQWLVKAPLQSKLIMQVHDELVLEVIEDELFVVKENLIILMENTFKLDVPLKVEVGVGK HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCEEEECCEEEEECCCC NWDQAH CCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]