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The map label for this gene is uvrD [H]

Identifier: 114330786

GI number: 114330786

Start: 789463

End: 791709

Strand: Direct

Name: uvrD [H]

Synonym: Neut_0773

Alternate gene names: 114330786

Gene position: 789463-791709 (Clockwise)

Preceding gene: 114330785

Following gene: 114330791

Centisome position: 29.67

GC content: 49.0

Gene sequence:

>2247_bases
ATGACTGCTCTACTTACTGATCTCAACCCGGAACAGCTGGAAGCCGTAACCTGGCCACACCAGTCCGCTCTGATACTGGC
GGGTGCCGGAAGTGGCAAAACCCGAGTGCTGACTACACGGATTGCGTATTTACTGCAATCAGGCCTTACTCAGCCGCAGA
ATCTTCTGGCAGTTACATTCACTAATAAAGCGGCACGTGAAATGGTCGCGCGTATCAGCACGATGCTGCCTATCAATATT
CGTGGCATGTGGATTGGCACATTTCATGGTTTGTGTCATCGCATGCTGCGTGCCCATTATGAAGATGCAGGGCTGCCGCA
GGCATTCCAAATACTGGATATGGCCGATCAGCTGGCTGTTATCAAGCGAATACTGAAAGAGCGTTCGTTGGATGAAAAAG
TGCTGGCACCCAGACAACTGCAATGGTTTATCAATAACGCCAAGGAAGAAGGATTACGAGCCTCACAGGTTGATACGCGT
GGCAGTTTCAATCGGACTCTGGCTGAATGCTATCAGGTGTACGAAGCTGTCAGTATGCGGGGAGGTACTGTTGATTTTGC
AGAACTGTTGCTGCGTTGCTATGAGTTGTTGAGTCGCAATGAAATTCTACGGGACCATTATCGTGGCCGTTTCGAACATA
TATTGGTAGATGAATTTCAGGATACCAACCGATTGCAATATCAATGGATCAAATTACTGGCAGGCTCGGGCTCACAACAA
CATGCTGCTATTTTTGCTGTCGGGGATGATGACCAAAGCATCTATGCATTCCGGGGAGCGCATGTTGGAAATATGCGTGA
TCTTGAGAAAGATTTCAGTGTACCCAAAATTATCAGACTGGAACAGAATTATCGCTCGCAAGGCAATATTCTGGACGCAG
CCAATGCACTGATCGAGAATAACAAAGGGCGTCTAGGTAAGAATCTGTGGACTGCTGCGGGGAAAGGTGAGTCTGTGCGG
ATTTATTACGCCGCTACGGATGTGGATGAAACCAGCTTTATTGTTGATGAAATCAAGGCTCTGCACGCTGAAGGATTGTC
CCTCTCCGAGATAGCTCTGCTTTATCGTTCCAATGCGCAATCACGGGTGCTGGAACACGGTTTGTTCAATGCATCTATTT
CTTACCGTGTTTACGGTGGTATGCGTTTTTTCGATCGGCAGGAAGTAAAGCATGCACTGGCTTATTTGCGACTGGTTGCT
TCGCCGGATGATGACAATGCCCTACTGCGGGTTATCAATTTTCCTCCCAGAGGAATTGGTGCACGCACATTGGAGCAATT
GCAGGACCAGGCGGAAATACTTGGAACCAGTCTTTGGCAAGCGGCACTTAAGGTATGCGAGGGAAGGAAAGCTGTTACCA
CCAGGGGTGACCAGCCAGGAAGAGGGATAGCAGGTTTTGTTAATCTGGTTCTATCCATGCAGCAGGCATGTGAAGGCCAG
CCGTTGCCGGAAATAATCCGGCGTGTAGTGGATCAGAGTGGGCTGGTGGCACATTACCAGGCTGAGCGTGAGGGTAACAA
TGAGCGCCTGGAAAATCTTAAAGAGCTGGTCAATGCTGCGACCAGTTTTGTGCATGAAGCCGAAGATGACAGTCTCACGG
CTTTTCTGGCACATGCCTCACTTGAAGGTGGTGAGCATCAGGCGGAGGGCTATCAGGATGCAGTTCAATTGATGACAGTG
CATGCTGCAAAAGGATTGGAATTTCATTCAGTATTTATCAGTGGCCTGGAGGAAGGGTTGTTTCCTCATGAAAACAGTCG
TAATGAGCCGAATGGGCTGGAAGAAGAAAGACGTCTGATGTATGTGGCAATGACGCGCGCGCGTCAACGCTTGTATCTGA
GCTATGCAGAGAGCCGTATGTTACACGGCCAGGTACGGGTGAATGTGCCTTCCAGGTTTGTTGATGAAATTCCGGAGGAG
TTGCTGAAAAGGTTGCGATCATCCGGTTTTTCTGGAGCAGGGTATCGGCAGGGACTGTCAGGAACCGGACAAAATACTGT
TAGCACTGCCTATTCTCGCCAAGGAAGAGGGAAGCATACACCAGTTTCAGCAGCAGTCAGGGTGGCCCCGCAAGGACTGA
ATACGGCGGGGTTGCATATTGGTCAGCAGGTATCACACACAAAATTTGGTACGGGCACTATTGTAAATTATGAGGGCAAT
GGATCTGATATGCGGGTTCAGGTGAATTTCCACCAGACAGGTACAAAATGGCTCTCACTTACCTATGCAAAACTGGTTCC
TTTATAA

Upstream 100 bases:

>100_bases
GCTGATTCTGGATCTGGTGGCAAAAAGCAAGAAATAGTTTTTCTGATATCCCCCCGTCTGGCTCTGATAGCAGACGGGAA
ATTCGGCTGTTCCCTCTTAT

Downstream 100 bases:

>100_bases
ATTGGATTATTTTGGTTCATCGTTCTCAATCTGGCTGATATTTTCCTTGTTTCCACCTGTGTCCTGATCAGATCTGGTAT
TTTCGTCTTGTTCGTAGTGA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MTALLTDLNPEQLEAVTWPHQSALILAGAGSGKTRVLTTRIAYLLQSGLTQPQNLLAVTFTNKAAREMVARISTMLPINI
RGMWIGTFHGLCHRMLRAHYEDAGLPQAFQILDMADQLAVIKRILKERSLDEKVLAPRQLQWFINNAKEEGLRASQVDTR
GSFNRTLAECYQVYEAVSMRGGTVDFAELLLRCYELLSRNEILRDHYRGRFEHILVDEFQDTNRLQYQWIKLLAGSGSQQ
HAAIFAVGDDDQSIYAFRGAHVGNMRDLEKDFSVPKIIRLEQNYRSQGNILDAANALIENNKGRLGKNLWTAAGKGESVR
IYYAATDVDETSFIVDEIKALHAEGLSLSEIALLYRSNAQSRVLEHGLFNASISYRVYGGMRFFDRQEVKHALAYLRLVA
SPDDDNALLRVINFPPRGIGARTLEQLQDQAEILGTSLWQAALKVCEGRKAVTTRGDQPGRGIAGFVNLVLSMQQACEGQ
PLPEIIRRVVDQSGLVAHYQAEREGNNERLENLKELVNAATSFVHEAEDDSLTAFLAHASLEGGEHQAEGYQDAVQLMTV
HAAKGLEFHSVFISGLEEGLFPHENSRNEPNGLEEERRLMYVAMTRARQRLYLSYAESRMLHGQVRVNVPSRFVDEIPEE
LLKRLRSSGFSGAGYRQGLSGTGQNTVSTAYSRQGRGKHTPVSAAVRVAPQGLNTAGLHIGQQVSHTKFGTGTIVNYEGN
GSDMRVQVNFHQTGTKWLSLTYAKLVPL

Sequences:

>Translated_748_residues
MTALLTDLNPEQLEAVTWPHQSALILAGAGSGKTRVLTTRIAYLLQSGLTQPQNLLAVTFTNKAAREMVARISTMLPINI
RGMWIGTFHGLCHRMLRAHYEDAGLPQAFQILDMADQLAVIKRILKERSLDEKVLAPRQLQWFINNAKEEGLRASQVDTR
GSFNRTLAECYQVYEAVSMRGGTVDFAELLLRCYELLSRNEILRDHYRGRFEHILVDEFQDTNRLQYQWIKLLAGSGSQQ
HAAIFAVGDDDQSIYAFRGAHVGNMRDLEKDFSVPKIIRLEQNYRSQGNILDAANALIENNKGRLGKNLWTAAGKGESVR
IYYAATDVDETSFIVDEIKALHAEGLSLSEIALLYRSNAQSRVLEHGLFNASISYRVYGGMRFFDRQEVKHALAYLRLVA
SPDDDNALLRVINFPPRGIGARTLEQLQDQAEILGTSLWQAALKVCEGRKAVTTRGDQPGRGIAGFVNLVLSMQQACEGQ
PLPEIIRRVVDQSGLVAHYQAEREGNNERLENLKELVNAATSFVHEAEDDSLTAFLAHASLEGGEHQAEGYQDAVQLMTV
HAAKGLEFHSVFISGLEEGLFPHENSRNEPNGLEEERRLMYVAMTRARQRLYLSYAESRMLHGQVRVNVPSRFVDEIPEE
LLKRLRSSGFSGAGYRQGLSGTGQNTVSTAYSRQGRGKHTPVSAAVRVAPQGLNTAGLHIGQQVSHTKFGTGTIVNYEGN
GSDMRVQVNFHQTGTKWLSLTYAKLVPL
>Mature_747_residues
TALLTDLNPEQLEAVTWPHQSALILAGAGSGKTRVLTTRIAYLLQSGLTQPQNLLAVTFTNKAAREMVARISTMLPINIR
GMWIGTFHGLCHRMLRAHYEDAGLPQAFQILDMADQLAVIKRILKERSLDEKVLAPRQLQWFINNAKEEGLRASQVDTRG
SFNRTLAECYQVYEAVSMRGGTVDFAELLLRCYELLSRNEILRDHYRGRFEHILVDEFQDTNRLQYQWIKLLAGSGSQQH
AAIFAVGDDDQSIYAFRGAHVGNMRDLEKDFSVPKIIRLEQNYRSQGNILDAANALIENNKGRLGKNLWTAAGKGESVRI
YYAATDVDETSFIVDEIKALHAEGLSLSEIALLYRSNAQSRVLEHGLFNASISYRVYGGMRFFDRQEVKHALAYLRLVAS
PDDDNALLRVINFPPRGIGARTLEQLQDQAEILGTSLWQAALKVCEGRKAVTTRGDQPGRGIAGFVNLVLSMQQACEGQP
LPEIIRRVVDQSGLVAHYQAEREGNNERLENLKELVNAATSFVHEAEDDSLTAFLAHASLEGGEHQAEGYQDAVQLMTVH
AAKGLEFHSVFISGLEEGLFPHENSRNEPNGLEEERRLMYVAMTRARQRLYLSYAESRMLHGQVRVNVPSRFVDEIPEEL
LKRLRSSGFSGAGYRQGLSGTGQNTVSTAYSRQGRGKHTPVSAAVRVAPQGLNTAGLHIGQQVSHTKFGTGTIVNYEGNG
SDMRVQVNFHQTGTKWLSLTYAKLVPL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=751, Percent_Identity=49.5339547270306, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=37.962962962963, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=343, Percent_Identity=27.9883381924198, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=750, Percent_Identity=27.6, Blast_Score=234, Evalue=3e-62,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83361; Mature: 83229

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALLTDLNPEQLEAVTWPHQSALILAGAGSGKTRVLTTRIAYLLQSGLTQPQNLLAVTF
CCCEECCCCHHHHHEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
TNKAAREMVARISTMLPINIRGMWIGTFHGLCHRMLRAHYEDAGLPQAFQILDMADQLAV
CCHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IKRILKERSLDEKVLAPRQLQWFINNAKEEGLRASQVDTRGSFNRTLAECYQVYEAVSMR
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCC
GGTVDFAELLLRCYELLSRNEILRDHYRGRFEHILVDEFQDTNRLQYQWIKLLAGSGSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
HAAIFAVGDDDQSIYAFRGAHVGNMRDLEKDFSVPKIIRLEQNYRSQGNILDAANALIEN
CEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHC
NKGRLGKNLWTAAGKGESVRIYYAATDVDETSFIVDEIKALHAEGLSLSEIALLYRSNAQ
CCCCCCCHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
SRVLEHGLFNASISYRVYGGMRFFDRQEVKHALAYLRLVASPDDDNALLRVINFPPRGIG
HHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ARTLEQLQDQAEILGTSLWQAALKVCEGRKAVTTRGDQPGRGIAGFVNLVLSMQQACEGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCC
PLPEIIRRVVDQSGLVAHYQAEREGNNERLENLKELVNAATSFVHEAEDDSLTAFLAHAS
CHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LEGGEHQAEGYQDAVQLMTVHAAKGLEFHSVFISGLEEGLFPHENSRNEPNGLEEERRLM
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YVAMTRARQRLYLSYAESRMLHGQVRVNVPSRFVDEIPEELLKRLRSSGFSGAGYRQGLS
HHHHHHHHHHHHHHHHHHHHEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
GTGQNTVSTAYSRQGRGKHTPVSAAVRVAPQGLNTAGLHIGQQVSHTKFGTGTIVNYEGN
CCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCHHHHHCCCHHCCCCCCCCEEEEEECC
GSDMRVQVNFHQTGTKWLSLTYAKLVPL
CCEEEEEEEEECCCCHHHEEHHHHHCCC
>Mature Secondary Structure 
TALLTDLNPEQLEAVTWPHQSALILAGAGSGKTRVLTTRIAYLLQSGLTQPQNLLAVTF
CCEECCCCHHHHHEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
TNKAAREMVARISTMLPINIRGMWIGTFHGLCHRMLRAHYEDAGLPQAFQILDMADQLAV
CCHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IKRILKERSLDEKVLAPRQLQWFINNAKEEGLRASQVDTRGSFNRTLAECYQVYEAVSMR
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCC
GGTVDFAELLLRCYELLSRNEILRDHYRGRFEHILVDEFQDTNRLQYQWIKLLAGSGSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
HAAIFAVGDDDQSIYAFRGAHVGNMRDLEKDFSVPKIIRLEQNYRSQGNILDAANALIEN
CEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHC
NKGRLGKNLWTAAGKGESVRIYYAATDVDETSFIVDEIKALHAEGLSLSEIALLYRSNAQ
CCCCCCCHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
SRVLEHGLFNASISYRVYGGMRFFDRQEVKHALAYLRLVASPDDDNALLRVINFPPRGIG
HHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ARTLEQLQDQAEILGTSLWQAALKVCEGRKAVTTRGDQPGRGIAGFVNLVLSMQQACEGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCC
PLPEIIRRVVDQSGLVAHYQAEREGNNERLENLKELVNAATSFVHEAEDDSLTAFLAHAS
CHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LEGGEHQAEGYQDAVQLMTVHAAKGLEFHSVFISGLEEGLFPHENSRNEPNGLEEERRLM
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YVAMTRARQRLYLSYAESRMLHGQVRVNVPSRFVDEIPEELLKRLRSSGFSGAGYRQGLS
HHHHHHHHHHHHHHHHHHHHEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
GTGQNTVSTAYSRQGRGKHTPVSAAVRVAPQGLNTAGLHIGQQVSHTKFGTGTIVNYEGN
CCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCHHHHHCCCHHCCCCCCCCEEEEEECC
GSDMRVQVNFHQTGTKWLSLTYAKLVPL
CCEEEEEEEEECCCCHHHEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]