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The map label for this gene is ruvB

Identifier: 114330311

GI number: 114330311

Start: 294629

End: 295669

Strand: Direct

Name: ruvB

Synonym: Neut_0284

Alternate gene names: 114330311

Gene position: 294629-295669 (Clockwise)

Preceding gene: 114330310

Following gene: 114330312

Centisome position: 11.07

GC content: 49.86

Gene sequence:

>1041_bases
ATGATTGAATCGGATCGGATTATCACTGCCTCTCCGTTTTCTTCCCAAGAAGAAGTAATAGAACGCGCACTTCGTCCTGT
TCAGCTGGATGACTATGTCGGTCAGGAAAAGATCCGTGAGCAATTAAAGATTTTTATTGAGGCTGCCAGACAAAGGCAAG
AAGCACTGGATCATGTGCTGCTGTTTGGCCCGCCCGGATTGGGTAAAACGACACTGGCTCATATTATTGCTCGCGAAATG
GGTGTAAATTTGCGCCATACTTCCGGCCCAGTACTAGAGCGCGCAGGTGATCTGGCCGCTTTGCTGACCAATCTGGAAGC
CAATGATGTATTGTTCATTGATGAGATTCACCGGCTTTCTCCCGTTGTTGAGGAAATTCTGTATCCGGCGATGGAGGATT
ACCAGCTGGATATCATGATCGGCGAGGGAGTGGCAGCAAGATCAGTCAAGATTGATTTGCCAAGTTTTACTCTAGTGGGG
GCGACTACGCGAGCGGGTATGTTGACCAACCCACTGAGAGACCGCTTTGGAATCGTTTCCCGCCTGGAATTTTATACTTC
AGATGAGCTCAGCAAGATTGTTACCCGTTCTGCCGGGTTACTTGATGTCGATATTACTGCCGATGGCGCCAGAGAGATTG
CATGCAGGTCACGGGGCACGCCACGGATCGCCAATCGATTACTGCGCAGAGTCCGTGATTTTGCTGAAGTCCGGGCGAAC
GGACGTATTGATCGCCAGGTAGCAGATGCTGCATTGCAGATGCTGGATGTTGATGCTGCTGGTCTGGATGTACTGGATAG
AAAGCTTCTGCTAGCAGTGCTGGAAAAATTTGGCGGTGGTCCGGTAGGAGTGGATAATCTTGCTGCGGCTATCAATGAAG
AGCGTGACACGATTGAAGAAGTGCTGGAGCCTTATCTTATTCAGCAGGGTTTTCTGCAGCGTACGCCTCGTGGCCGAATG
GCAACAACCATGACTTATCAGCATTTCGATATCATCCCTTTACAGCAAGCCGCTACACCCGGTCTGTTTAACCCAGATTA
A

Upstream 100 bases:

>100_bases
GGCATTAGAGCAATTGTCGGAAGGTGTTACGGTATCTGACGGCATTATGCAGTCACTTAAATTTTTGTCGAAAGTAAAAT
AACGTTTTGGCTGATCTTAA

Downstream 100 bases:

>100_bases
AAGCTAGTAATAACCCGCAAACCAAGATTCAATATCTTTCTGAAGAGTATTTTTACAAAAATGAAAGCTGCTTTTTTTTA
TCCCGTGCGTGTCTACTACC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MIESDRIITASPFSSQEEVIERALRPVQLDDYVGQEKIREQLKIFIEAARQRQEALDHVLLFGPPGLGKTTLAHIIAREM
GVNLRHTSGPVLERAGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGVAARSVKIDLPSFTLVG
ATTRAGMLTNPLRDRFGIVSRLEFYTSDELSKIVTRSAGLLDVDITADGAREIACRSRGTPRIANRLLRRVRDFAEVRAN
GRIDRQVADAALQMLDVDAAGLDVLDRKLLLAVLEKFGGGPVGVDNLAAAINEERDTIEEVLEPYLIQQGFLQRTPRGRM
ATTMTYQHFDIIPLQQAATPGLFNPD

Sequences:

>Translated_346_residues
MIESDRIITASPFSSQEEVIERALRPVQLDDYVGQEKIREQLKIFIEAARQRQEALDHVLLFGPPGLGKTTLAHIIAREM
GVNLRHTSGPVLERAGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGVAARSVKIDLPSFTLVG
ATTRAGMLTNPLRDRFGIVSRLEFYTSDELSKIVTRSAGLLDVDITADGAREIACRSRGTPRIANRLLRRVRDFAEVRAN
GRIDRQVADAALQMLDVDAAGLDVLDRKLLLAVLEKFGGGPVGVDNLAAAINEERDTIEEVLEPYLIQQGFLQRTPRGRM
ATTMTYQHFDIIPLQQAATPGLFNPD
>Mature_346_residues
MIESDRIITASPFSSQEEVIERALRPVQLDDYVGQEKIREQLKIFIEAARQRQEALDHVLLFGPPGLGKTTLAHIIAREM
GVNLRHTSGPVLERAGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGVAARSVKIDLPSFTLVG
ATTRAGMLTNPLRDRFGIVSRLEFYTSDELSKIVTRSAGLLDVDITADGAREIACRSRGTPRIANRLLRRVRDFAEVRAN
GRIDRQVADAALQMLDVDAAGLDVLDRKLLLAVLEKFGGGPVGVDNLAAAINEERDTIEEVLEPYLIQQGFLQRTPRGRM
ATTMTYQHFDIIPLQQAATPGLFNPD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=333, Percent_Identity=71.4714714714715, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1787119, Length=240, Percent_Identity=27.5, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_NITEC (Q0AJA3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746533.1
- ProteinModelPortal:   Q0AJA3
- SMR:   Q0AJA3
- STRING:   Q0AJA3
- GeneID:   4272057
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0284
- NMPDR:   fig|335283.3.peg.2239
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q0AJA3
- ProtClustDB:   PRK00080
- BioCyc:   NEUT335283:NEUT_0284-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38334; Mature: 38334

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESDRIITASPFSSQEEVIERALRPVQLDDYVGQEKIREQLKIFIEAARQRQEALDHVL
CCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEE
LFGPPGLGKTTLAHIIAREMGVNLRHTSGPVLERAGDLAALLTNLEANDVLFIDEIHRLS
EECCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
PVVEEILYPAMEDYQLDIMIGEGVAARSVKIDLPSFTLVGATTRAGMLTNPLRDRFGIVS
HHHHHHHHCCCCCCEEEEEECCCCEECEEEEECCCEEEEECCHHCCHHHHHHHHHHHHHH
RLEFYTSDELSKIVTRSAGLLDVDITADGAREIACRSRGTPRIANRLLRRVRDFAEVRAN
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GRIDRQVADAALQMLDVDAAGLDVLDRKLLLAVLEKFGGGPVGVDNLAAAINEERDTIEE
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VLEPYLIQQGFLQRTPRGRMATTMTYQHFDIIPLQQAATPGLFNPD
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEECHHHCCCCCCCCC
>Mature Secondary Structure
MIESDRIITASPFSSQEEVIERALRPVQLDDYVGQEKIREQLKIFIEAARQRQEALDHVL
CCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEE
LFGPPGLGKTTLAHIIAREMGVNLRHTSGPVLERAGDLAALLTNLEANDVLFIDEIHRLS
EECCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
PVVEEILYPAMEDYQLDIMIGEGVAARSVKIDLPSFTLVGATTRAGMLTNPLRDRFGIVS
HHHHHHHHCCCCCCEEEEEECCCCEECEEEEECCCEEEEECCHHCCHHHHHHHHHHHHHH
RLEFYTSDELSKIVTRSAGLLDVDITADGAREIACRSRGTPRIANRLLRRVRDFAEVRAN
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GRIDRQVADAALQMLDVDAAGLDVLDRKLLLAVLEKFGGGPVGVDNLAAAINEERDTIEE
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VLEPYLIQQGFLQRTPRGRMATTMTYQHFDIIPLQQAATPGLFNPD
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEECHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA