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The map label for this gene is ruvA

Identifier: 114330310

GI number: 114330310

Start: 294026

End: 294610

Strand: Direct

Name: ruvA

Synonym: Neut_0283

Alternate gene names: 114330310

Gene position: 294026-294610 (Clockwise)

Preceding gene: 114330309

Following gene: 114330311

Centisome position: 11.05

GC content: 46.32

Gene sequence:

>585_bases
GTGATTGGCAGAATTACCGGGTTGCTGCTTGAAAAACATCCTCCGCTGGTGTTGGTTGATGTACAAGGTACCGGTTATGA
AATTGACGTGCCGATGAGTACTTTCTGCAAGTTGCCTGATATTGGCAAAAAGGTAACGCTCCATACCCATTTCTGGGTAC
GAGAGGATGTACATTTGCTGTTTGGTTTTATGACGGAGCAGGAACGTGCGCTTTTTCGCCAGCTTACAAAAATTTCTGGT
ATTGGTGCGAGAACAGGTTTGGCGATTTTGTCCGGCTTGAGCGTCACAGATCTCCATCAAGCTGTGGTATCGCAGGACAG
TATTCGCCTAACCAAAATCCCCGGAATTGGAAAGAAGACGGCGGAGCGTTTATTACTGGAGTTGCGCGATAAGATCGATC
CAGTAGCGATCCTTTCTGAAGCGGGAGCGGCTGCATCTAACGTTGACAAAGACATTCTTTCTGCACTATTAGCACTGGGC
TATAATGGCCGCGAAGTTAACCGGGCATTAGAGCAATTGTCGGAAGGTGTTACGGTATCTGACGGCATTATGCAGTCACT
TAAATTTTTGTCGAAAGTAAAATAA

Upstream 100 bases:

>100_bases
ATGCGGCTGATGCGTTGGCCTGTGCCATTTGCCATGCTCATGGAGGAACAGGCTTGCTTGCCCTGAGTGTGCGTAATCAT
CTGAAACGAGGTGGTTGTCC

Downstream 100 bases:

>100_bases
CGTTTTGGCTGATCTTAAATGATTGAATCGGATCGGATTATCACTGCCTCTCCGTTTTCTTCCCAAGAAGAAGTAATAGA
ACGCGCACTTCGTCCTGTTC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MIGRITGLLLEKHPPLVLVDVQGTGYEIDVPMSTFCKLPDIGKKVTLHTHFWVREDVHLLFGFMTEQERALFRQLTKISG
IGARTGLAILSGLSVTDLHQAVVSQDSIRLTKIPGIGKKTAERLLLELRDKIDPVAILSEAGAAASNVDKDILSALLALG
YNGREVNRALEQLSEGVTVSDGIMQSLKFLSKVK

Sequences:

>Translated_194_residues
MIGRITGLLLEKHPPLVLVDVQGTGYEIDVPMSTFCKLPDIGKKVTLHTHFWVREDVHLLFGFMTEQERALFRQLTKISG
IGARTGLAILSGLSVTDLHQAVVSQDSIRLTKIPGIGKKTAERLLLELRDKIDPVAILSEAGAAASNVDKDILSALLALG
YNGREVNRALEQLSEGVTVSDGIMQSLKFLSKVK
>Mature_194_residues
MIGRITGLLLEKHPPLVLVDVQGTGYEIDVPMSTFCKLPDIGKKVTLHTHFWVREDVHLLFGFMTEQERALFRQLTKISG
IGARTGLAILSGLSVTDLHQAVVSQDSIRLTKIPGIGKKTAERLLLELRDKIDPVAILSEAGAAASNVDKDILSALLALG
YNGREVNRALEQLSEGVTVSDGIMQSLKFLSKVK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=185, Percent_Identity=43.7837837837838, Blast_Score=167, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_NITEC (Q0AJA4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746532.1
- ProteinModelPortal:   Q0AJA4
- SMR:   Q0AJA4
- STRING:   Q0AJA4
- GeneID:   4272126
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0283
- NMPDR:   fig|335283.3.peg.2238
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- PhylomeDB:   Q0AJA4
- ProtClustDB:   PRK00116
- BioCyc:   NEUT335283:NEUT_0283-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21155; Mature: 21155

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRITGLLLEKHPPLVLVDVQGTGYEIDVPMSTFCKLPDIGKKVTLHTHFWVREDVHLL
CCCHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHCCCCCCCEEEEEEEHHHHHHHHHH
FGFMTEQERALFRQLTKISGIGARTGLAILSGLSVTDLHQAVVSQDSIRLTKIPGIGKKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHH
AERLLLELRDKIDPVAILSEAGAAASNVDKDILSALLALGYNGREVNRALEQLSEGVTVS
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
DGIMQSLKFLSKVK
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRITGLLLEKHPPLVLVDVQGTGYEIDVPMSTFCKLPDIGKKVTLHTHFWVREDVHLL
CCCHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHCCCCCCCEEEEEEEHHHHHHHHHH
FGFMTEQERALFRQLTKISGIGARTGLAILSGLSVTDLHQAVVSQDSIRLTKIPGIGKKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHH
AERLLLELRDKIDPVAILSEAGAAASNVDKDILSALLALGYNGREVNRALEQLSEGVTVS
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
DGIMQSLKFLSKVK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA