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The map label for this gene is ruvC

Identifier: 114330309

GI number: 114330309

Start: 293493

End: 294029

Strand: Direct

Name: ruvC

Synonym: Neut_0282

Alternate gene names: 114330309

Gene position: 293493-294029 (Clockwise)

Preceding gene: 114330308

Following gene: 114330310

Centisome position: 11.03

GC content: 50.65

Gene sequence:

>537_bases
TTGTTACCTTCCGGCAAGAGCATCCGCATACTGGGGATTGACCCTGGCTTACGTATAACAGGTTTTGGTGTTATCGAAAA
AAATGGAAGCCAACTAACCTACCTTGGGAGTGGCTGCGTAGTAACCGAAAAATCCGGTTTGCCGGATCGCTTGAAGACCA
TCCTTGATGGACTGAATGAAATTATTTTGCAGCACCAACCGGAGCAGGTTGCTGTAGAGCAGGTTTTTGTCAATATCAAT
CCTAAATCGACGCTGTTGCTCGGACAGGCTCGCGGTGCCGCAATTAGCGCAGCAGTTCTTCATGAACTTTCCGTATACGA
ATATACTGCTCTGCAAATCAAGCAATCTGTAGTGGGGAATGGCCATGCCCGGAAAGAGCAGGTGCAGGAAATGGTGATGC
GATTGTTGAGGCTGGGAGAACGCCCTCGCCCGGATGCGGCTGATGCGTTGGCCTGTGCCATTTGCCATGCTCATGGAGGA
ACAGGCTTGCTTGCCCTGAGTGTGCGTAATCATCTGAAACGAGGTGGTTGTCCGTGA

Upstream 100 bases:

>100_bases
AGGGTGATGATGCAGCCAAAATGCAGAAGCTGTTGGATGCTCTGGAAGATATAGACGATGTACAGGATGTTTATACTACT
GCAGTCTTAGACATCTAATC

Downstream 100 bases:

>100_bases
TTGGCAGAATTACCGGGTTGCTGCTTGAAAAACATCCTCCGCTGGTGTTGGTTGATGTACAAGGTACCGGTTATGAAATT
GACGTGCCGATGAGTACTTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MLPSGKSIRILGIDPGLRITGFGVIEKNGSQLTYLGSGCVVTEKSGLPDRLKTILDGLNEIILQHQPEQVAVEQVFVNIN
PKSTLLLGQARGAAISAAVLHELSVYEYTALQIKQSVVGNGHARKEQVQEMVMRLLRLGERPRPDAADALACAICHAHGG
TGLLALSVRNHLKRGGCP

Sequences:

>Translated_178_residues
MLPSGKSIRILGIDPGLRITGFGVIEKNGSQLTYLGSGCVVTEKSGLPDRLKTILDGLNEIILQHQPEQVAVEQVFVNIN
PKSTLLLGQARGAAISAAVLHELSVYEYTALQIKQSVVGNGHARKEQVQEMVMRLLRLGERPRPDAADALACAICHAHGG
TGLLALSVRNHLKRGGCP
>Mature_178_residues
MLPSGKSIRILGIDPGLRITGFGVIEKNGSQLTYLGSGCVVTEKSGLPDRLKTILDGLNEIILQHQPEQVAVEQVFVNIN
PKSTLLLGQARGAAISAAVLHELSVYEYTALQIKQSVVGNGHARKEQVQEMVMRLLRLGERPRPDAADALACAICHAHGG
TGLLALSVRNHLKRGGCP

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=167, Percent_Identity=56.8862275449102, Blast_Score=156, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_NITEC (Q0AJA5)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746531.1
- ProteinModelPortal:   Q0AJA5
- SMR:   Q0AJA5
- STRING:   Q0AJA5
- GeneID:   4272125
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0282
- NMPDR:   fig|335283.3.peg.2237
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q0AJA5
- ProtClustDB:   PRK00039
- BioCyc:   NEUT335283:NEUT_0282-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19031; Mature: 19031

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSGKSIRILGIDPGLRITGFGVIEKNGSQLTYLGSGCVVTEKSGLPDRLKTILDGLNE
CCCCCCEEEEEEECCCCEEEEEEEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHH
IILQHQPEQVAVEQVFVNINPKSTLLLGQARGAAISAAVLHELSVYEYTALQIKQSVVGN
HHHCCCCHHEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHARKEQVQEMVMRLLRLGERPRPDAADALACAICHAHGGTGLLALSVRNHLKRGGCP
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCC
>Mature Secondary Structure
MLPSGKSIRILGIDPGLRITGFGVIEKNGSQLTYLGSGCVVTEKSGLPDRLKTILDGLNE
CCCCCCEEEEEEECCCCEEEEEEEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHH
IILQHQPEQVAVEQVFVNINPKSTLLLGQARGAAISAAVLHELSVYEYTALQIKQSVVGN
HHHCCCCHHEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHARKEQVQEMVMRLLRLGERPRPDAADALACAICHAHGGTGLLALSVRNHLKRGGCP
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA