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The map label for this gene is cusS [H]

Identifier: 114330052

GI number: 114330052

Start: 18615

End: 20027

Strand: Reverse

Name: cusS [H]

Synonym: Neut_0017

Alternate gene names: 114330052

Gene position: 20027-18615 (Counterclockwise)

Preceding gene: 114330053

Following gene: 114330050

Centisome position: 0.75

GC content: 49.33

Gene sequence:

>1413_bases
GTGGCACGCCGCCCTGCGTCGCTGGCCCTGCGTCTGACCGTATCCATTGGAGCGGTCATCACAGTGGTGCTTCTCACGTT
TGGATGGATGGTCCAACGATCCATTAATAGTCACTTCGTTCAGCAGGATGTTGATGAGCTTAATGCTGTTGTTCAATCGC
TGACGCAGTCGCTCTCCGAGCCGTCAGCCAGTCAAGAGTCGGATGTATTGAAGCGTCGGCTGGCGGCTGCGATCTCAGGT
CATCGTAATGCACAATTTCGAGTGTCGGATAGTCATGGCAATGTGATTTACGCTACGCCTAATTCGGATCTGGATGGTTT
TACACGGTTGGTTCCCTTTACCGGCGTAATCACCATTGACTCGGTGAAAATCTGGCGGGATAAGGGAGAGACCTATCGAG
GTGCTGTTGTGCAGCTAATGAACAAAGGTCTTCAAGGCGCCGAGCCTTTAACCTTAGCCGTTGCCACTGGCATCAATTTT
CACTTGCACTATCTTGAAAGCTTTCGCAGTTACCTGAGAGTTATTACGACCGCAGCCTGTCTGATTGCTATTCTGGCGAC
ATGGTTTGCTGTGTATCAGGGTCATGCGCCGATACGGCGCATCAGCCGAGAGATTCGTCGAATCAAATCTGATCAGCTTT
TTATTCGGCTCGCACCTGGTACCGTTCCGGTGGAGTTAACTGAACTGGCCGTGTCATTCAACGACATGCTGGATCGCATC
GAAGATGGTTTTCAACGCCTGTCGAACTTTTCGGCTGATATCGCTCATGAGCTGCGTACGCCCATAACGAATCTGAAAAC
GCAGACGGAAGTGGCACTCTCTCAATCGCGCGATGTCGAGCAGTATCGAGAAATTTTATATTCGAATCTGGAAGAATACG
AACGTATGGCCAAAATGGTCGGCGATATGCTGTTTTTGGCGCAAGCCGATAATAATCAGCTCAAACCGGAACTGGTCAGC
GTGAATTTGGTTACTGAAGTCCAGGAGCTATTTGATTACTTCGAGGCATGGGCCGAAGAACGCTCCGTTTCATTGATATG
TAAAGGTCCAGTTATATGTGTTCAGGGTGATAGATTGATGATGCGCCGCGCGCTAAGCAATCTTTTATCCAATGCCATAC
GTTACACCCCTCCAGGGAGAGCAGTTACAGTCTCGCTAGCTGCTAACAGCAACGACACGATTGCTATTAGTGTAGAAAAC
CCGGGACATACGATCAATTCCGAGCATCTACCAAGATTGTTTGACCGGTTTTACCGCGCGGATCCTTCACGTCAACGTAA
AGGGGACGGAGCTGGTTTAGGTCTAGCTATTGTCAAGTCAATTATTGACGCACATGGCGGCACCATTACTGCAAAAGAGG
TGGATGGAAATATGATTTTTGAGGTCGCTCTTCCTAAAGCGACGGCCAAGTAA

Upstream 100 bases:

>100_bases
GCCTGCGCGCCAAAATCGATGACGCCTTTGAACCCAAGCTGATCCATACCGTTCGCGGTATGGGATATATGCTTGATGTC
AGCAGCGTAGGTTGAAAAGA

Downstream 100 bases:

>100_bases
ATTTTTAACTAGGTATCGCTGATTAACAATGTGCTACACGCTGTAGCGGCTCTGGTTATAGCGGTGGGTACAGAAATAAT
TTGGCTGAGGAATACAACAT

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MARRPASLALRLTVSIGAVITVVLLTFGWMVQRSINSHFVQQDVDELNAVVQSLTQSLSEPSASQESDVLKRRLAAAISG
HRNAQFRVSDSHGNVIYATPNSDLDGFTRLVPFTGVITIDSVKIWRDKGETYRGAVVQLMNKGLQGAEPLTLAVATGINF
HLHYLESFRSYLRVITTAACLIAILATWFAVYQGHAPIRRISREIRRIKSDQLFIRLAPGTVPVELTELAVSFNDMLDRI
EDGFQRLSNFSADIAHELRTPITNLKTQTEVALSQSRDVEQYREILYSNLEEYERMAKMVGDMLFLAQADNNQLKPELVS
VNLVTEVQELFDYFEAWAEERSVSLICKGPVICVQGDRLMMRRALSNLLSNAIRYTPPGRAVTVSLAANSNDTIAISVEN
PGHTINSEHLPRLFDRFYRADPSRQRKGDGAGLGLAIVKSIIDAHGGTITAKEVDGNMIFEVALPKATAK

Sequences:

>Translated_470_residues
MARRPASLALRLTVSIGAVITVVLLTFGWMVQRSINSHFVQQDVDELNAVVQSLTQSLSEPSASQESDVLKRRLAAAISG
HRNAQFRVSDSHGNVIYATPNSDLDGFTRLVPFTGVITIDSVKIWRDKGETYRGAVVQLMNKGLQGAEPLTLAVATGINF
HLHYLESFRSYLRVITTAACLIAILATWFAVYQGHAPIRRISREIRRIKSDQLFIRLAPGTVPVELTELAVSFNDMLDRI
EDGFQRLSNFSADIAHELRTPITNLKTQTEVALSQSRDVEQYREILYSNLEEYERMAKMVGDMLFLAQADNNQLKPELVS
VNLVTEVQELFDYFEAWAEERSVSLICKGPVICVQGDRLMMRRALSNLLSNAIRYTPPGRAVTVSLAANSNDTIAISVEN
PGHTINSEHLPRLFDRFYRADPSRQRKGDGAGLGLAIVKSIIDAHGGTITAKEVDGNMIFEVALPKATAK
>Mature_469_residues
ARRPASLALRLTVSIGAVITVVLLTFGWMVQRSINSHFVQQDVDELNAVVQSLTQSLSEPSASQESDVLKRRLAAAISGH
RNAQFRVSDSHGNVIYATPNSDLDGFTRLVPFTGVITIDSVKIWRDKGETYRGAVVQLMNKGLQGAEPLTLAVATGINFH
LHYLESFRSYLRVITTAACLIAILATWFAVYQGHAPIRRISREIRRIKSDQLFIRLAPGTVPVELTELAVSFNDMLDRIE
DGFQRLSNFSADIAHELRTPITNLKTQTEVALSQSRDVEQYREILYSNLEEYERMAKMVGDMLFLAQADNNQLKPELVSV
NLVTEVQELFDYFEAWAEERSVSLICKGPVICVQGDRLMMRRALSNLLSNAIRYTPPGRAVTVSLAANSNDTIAISVENP
GHTINSEHLPRLFDRFYRADPSRQRKGDGAGLGLAIVKSIIDAHGGTITAKEVDGNMIFEVALPKATAK

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=489, Percent_Identity=45.1942740286299, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788279, Length=320, Percent_Identity=32.5, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=31.25, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786600, Length=199, Percent_Identity=35.1758793969849, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790346, Length=248, Percent_Identity=30.6451612903226, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790551, Length=271, Percent_Identity=28.4132841328413, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789403, Length=253, Percent_Identity=32.4110671936759, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=32.2448979591837, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=26.6375545851528, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=28.1938325991189, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=29.9559471365639, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=25.3424657534247, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=252, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787374, Length=311, Percent_Identity=24.7588424437299, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=24.8275862068966, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=26.3392857142857, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789149, Length=219, Percent_Identity=29.6803652968037, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=23.5807860262009, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52155; Mature: 52024

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPASLALRLTVSIGAVITVVLLTFGWMVQRSINSHFVQQDVDELNAVVQSLTQSLSE
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSASQESDVLKRRLAAAISGHRNAQFRVSDSHGNVIYATPNSDLDGFTRLVPFTGVITID
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHCCCCEEEEC
SVKIWRDKGETYRGAVVQLMNKGLQGAEPLTLAVATGINFHLHYLESFRSYLRVITTAAC
CEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LIAILATWFAVYQGHAPIRRISREIRRIKSDQLFIRLAPGTVPVELTELAVSFNDMLDRI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDGFQRLSNFSADIAHELRTPITNLKTQTEVALSQSRDVEQYREILYSNLEEYERMAKMV
HHHHHHHHCCCHHHHHHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GDMLFLAQADNNQLKPELVSVNLVTEVQELFDYFEAWAEERSVSLICKGPVICVQGDRLM
HHHEEEEECCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHH
MRRALSNLLSNAIRYTPPGRAVTVSLAANSNDTIAISVENPGHTINSEHLPRLFDRFYRA
HHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
DPSRQRKGDGAGLGLAIVKSIIDAHGGTITAKEVDGNMIFEVALPKATAK
CCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ARRPASLALRLTVSIGAVITVVLLTFGWMVQRSINSHFVQQDVDELNAVVQSLTQSLSE
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSASQESDVLKRRLAAAISGHRNAQFRVSDSHGNVIYATPNSDLDGFTRLVPFTGVITID
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHCCCCEEEEC
SVKIWRDKGETYRGAVVQLMNKGLQGAEPLTLAVATGINFHLHYLESFRSYLRVITTAAC
CEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LIAILATWFAVYQGHAPIRRISREIRRIKSDQLFIRLAPGTVPVELTELAVSFNDMLDRI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDGFQRLSNFSADIAHELRTPITNLKTQTEVALSQSRDVEQYREILYSNLEEYERMAKMV
HHHHHHHHCCCHHHHHHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GDMLFLAQADNNQLKPELVSVNLVTEVQELFDYFEAWAEERSVSLICKGPVICVQGDRLM
HHHEEEEECCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHH
MRRALSNLLSNAIRYTPPGRAVTVSLAANSNDTIAISVENPGHTINSEHLPRLFDRFYRA
HHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
DPSRQRKGDGAGLGLAIVKSIIDAHGGTITAKEVDGNMIFEVALPKATAK
CCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]