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The map label for this gene is cusR [H]

Identifier: 114330053

GI number: 114330053

Start: 20033

End: 20716

Strand: Reverse

Name: cusR [H]

Synonym: Neut_0018

Alternate gene names: 114330053

Gene position: 20716-20033 (Counterclockwise)

Preceding gene: 114330054

Following gene: 114330052

Centisome position: 0.78

GC content: 53.65

Gene sequence:

>684_bases
ATGAAATTGCTCGTCGTTGAAGATGAGATAAAAACTGGCGAATACGTACGCCAAGGGCTGGTTGAGTCCGGTTTTATAGT
CGATCTGGCCCGTACGGGCCTGGATGGGCACCACCTCGCCCTGACAGAGGACTATGATTTAATCATCCTAGATGTCATGC
TGCCAGATGTTGACGGTTGGCGTATTGTTCAAGCCTTGCGTGAAGCGAAATCGGCGACACCGGTATTGTTTTTGACGGCG
CGCGATAGCGTAGAGGACCGGGTTAAGGGTTTGGAACTAGGCGCCGATGATTATTTGATTAAACCTTTTGCATTTTCGGA
GCTGCTCGCACGAGTGCGTACACTGCTGCGCCGAGGCGCAGCGCCCGCTCTGAGTGATCAATTGCAAGTGGCCGACCTGG
TGCTGGACATTCCACGTCGGCGGGCGAGGCGGCAAGAGGTACGCATTAACCTGACAAACAAAGAATTTGCGCTTCTGGAG
CTTTTGGTGCGCCGCCAAGGTGAGGTGTTACCGCGCTCTCTGATTGCCTCACAGGTGTGGGACATGAATTTTGACAGCGA
CACCAACGTCATTGACGTGGCGATTCGGCGCCTGCGCGCCAAAATCGATGACGCCTTTGAACCCAAGCTGATCCATACCG
TTCGCGGTATGGGATATATGCTTGATGTCAGCAGCGTAGGTTGA

Upstream 100 bases:

>100_bases
TGCCACGGGTAGAGTCTCGTCATGAGTTCTGACGCTTCTAGGTCAGGGGGCAATCCCTTAAGTCAGGCACAAAACAGAAG
CCTATCGCAGGAGCGATCCC

Downstream 100 bases:

>100_bases
AAAGAGTGGCACGCCGCCCTGCGTCGCTGGCCCTGCGTCTGACCGTATCCATTGGAGCGGTCATCACAGTGGTGCTTCTC
ACGTTTGGATGGATGGTCCA

Product: two component heavy metal response transcriptional regulator, winged helix family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKLLVVEDEIKTGEYVRQGLVESGFIVDLARTGLDGHHLALTEDYDLIILDVMLPDVDGWRIVQALREAKSATPVLFLTA
RDSVEDRVKGLELGADDYLIKPFAFSELLARVRTLLRRGAAPALSDQLQVADLVLDIPRRRARRQEVRINLTNKEFALLE
LLVRRQGEVLPRSLIASQVWDMNFDSDTNVIDVAIRRLRAKIDDAFEPKLIHTVRGMGYMLDVSSVG

Sequences:

>Translated_227_residues
MKLLVVEDEIKTGEYVRQGLVESGFIVDLARTGLDGHHLALTEDYDLIILDVMLPDVDGWRIVQALREAKSATPVLFLTA
RDSVEDRVKGLELGADDYLIKPFAFSELLARVRTLLRRGAAPALSDQLQVADLVLDIPRRRARRQEVRINLTNKEFALLE
LLVRRQGEVLPRSLIASQVWDMNFDSDTNVIDVAIRRLRAKIDDAFEPKLIHTVRGMGYMLDVSSVG
>Mature_227_residues
MKLLVVEDEIKTGEYVRQGLVESGFIVDLARTGLDGHHLALTEDYDLIILDVMLPDVDGWRIVQALREAKSATPVLFLTA
RDSVEDRVKGLELGADDYLIKPFAFSELLARVRTLLRRGAAPALSDQLQVADLVLDIPRRRARRQEVRINLTNKEFALLE
LLVRRQGEVLPRSLIASQVWDMNFDSDTNVIDVAIRRLRAKIDDAFEPKLIHTVRGMGYMLDVSSVG

Specific function: Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA in response to increasing levels of copper ions, and probably silver ions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=70.4035874439462, Blast_Score=326, Evalue=9e-91,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=57.9908675799087, Blast_Score=255, Evalue=1e-69,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=41.1764705882353, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=38.6666666666667, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1790860, Length=220, Percent_Identity=39.0909090909091, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786911, Length=220, Percent_Identity=38.1818181818182, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786599, Length=221, Percent_Identity=35.7466063348416, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=35.2422907488987, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=32.7354260089686, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=33.0396475770925, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=37.5, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=35.1351351351351, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=29.646017699115, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=32.4675324675325, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=32.7102803738318, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=35.0877192982456, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=185, Percent_Identity=29.1891891891892, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR006291
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25603; Mature: 25603

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVEDEIKTGEYVRQGLVESGFIVDLARTGLDGHHLALTEDYDLIILDVMLPDVDGW
CEEEEEECCCCCHHHHHHHHHCCCCEEEHHHCCCCCCEEEEECCCCEEEEEEECCCCCHH
RIVQALREAKSATPVLFLTARDSVEDRVKGLELGADDYLIKPFAFSELLARVRTLLRRGA
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCCC
APALSDQLQVADLVLDIPRRRARRQEVRINLTNKEFALLELLVRRQGEVLPRSLIASQVW
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHH
DMNFDSDTNVIDVAIRRLRAKIDDAFEPKLIHTVRGMGYMLDVSSVG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure
MKLLVVEDEIKTGEYVRQGLVESGFIVDLARTGLDGHHLALTEDYDLIILDVMLPDVDGW
CEEEEEECCCCCHHHHHHHHHCCCCEEEHHHCCCCCCEEEEECCCCEEEEEEECCCCCHH
RIVQALREAKSATPVLFLTARDSVEDRVKGLELGADDYLIKPFAFSELLARVRTLLRRGA
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCCC
APALSDQLQVADLVLDIPRRRARRQEVRINLTNKEFALLELLVRRQGEVLPRSLIASQVW
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHH
DMNFDSDTNVIDVAIRRLRAKIDDAFEPKLIHTVRGMGYMLDVSSVG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]