Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 113969775

GI number: 113969775

Start: 1675222

End: 1677342

Strand: Direct

Name: topB [H]

Synonym: Shewmr4_1433

Alternate gene names: 113969775

Gene position: 1675222-1677342 (Clockwise)

Preceding gene: 113969772

Following gene: 113969777

Centisome position: 35.6

GC content: 51.34

Gene sequence:

>2121_bases
ATGATCCTCTATATTGCTGAAAAACCAAGCCTAGGGCGTGCAATCGCCGATGTGCTGCCTAAACCCCACAAAAAAGGTGA
CGGGTTTATCGAAGACGCAAACGGTAACTGTGTGTCTTGGTGTGTCGGGCACTTGCTCGAACAGGCTGAGCCCGATGCCT
ATAATCCCGAGTTTAAATCGTGGAAGTTTGAGCATTTACCCATAGTGCCAGAGAAGTGGCAGCTTAAACCTAAGGCCGCG
ACCCGCAGCCAACTCACAGTACTTAAAAAGCTGGTGAAGCAGGCCGATACTTTAGTGAATGCGGGTGATCCCGACAGGGA
AGGGCAGTTATTGGTTGATGAAGTGATCGCCTATTTGGGCGTGACGGGCGATAAATTACAGCAAACGCAGCGACTCCTTA
TCAGCGACTTAAACCCGCAGGCGGTGAAACGTGCCTTGTCACAACTCCGCAGTAATCGTGAGTTTATTCCTTTGTCGACC
TCGGCACTTGCCAGAAGCCGCGCCGATTGGCTCTATGGCATGAATATGACGCGGGCCTACACTATTCAAGGGAAAAAGGT
TGGCTATCAGGGCGTACTATCGGTCGGCCGAGTACAGACCCCGCTGCTTGGGCTTATCGTGCGCCGCGATGAGGAAATCG
CAAATTTTCAATCTAAGCCTTTTTATGAGGTTCTGGCCCACTTAGTGACGGACAAAGATCAGGCTTTCACGGCGAAGTGG
CAACCCAGTGAAGCCTGTTTACCCTATATGGATGAGGAAGGGCGCGTGCTCGCTAAGGGCCTAGCACAAAATGTGGTGCG
TCGGATCACCGATAAACCCGCCAAAGTGACCCAGTTAAGCACTAAGGATAAGCAGCAGAATCCGCCTTTACCCTATAGTT
TATCGGCGCTGCAAATAGATGCCGCCAAGCGCTTCGGTATGAGCGCCAAGGACGTGCTCGATACCTGCCAGAGCTTATAC
GAGCGCCATAAGCTAATCACTTATCCACGCTCAGACAGTCGTTATTTGCCCACCGAGCAGCATGGGCTTGCAAGTGGGGT
ATTAGCGGCCATTACGGGCGGTGCGCCCGAGTTAGTCGCACATGCTGAGGCGCCCAATCCTCGGCTTAAATCTAAGGCGT
GGGATGATAAAAAAGTCGATGCTCACCACGCCATTATTCCAACGGAAAAACAGCCCAATCTAGCTGGTTTAAGCCAGCGG
GAAAGGCAGCTTTATCTGCATATTGCTCGCCAGTATTTGGCGCAGTTTTACCCTGCCTATTGCTACAGTGAAACCGCGGT
CGAAGTGACGATCGAAGGTGGCCTTTTTAAAACTAAGGCAAAACAGGATAAGTCTCTCGGTTGGAAACAACTGTTTGCCA
GAGAAGAGAACGAACGAAAGGACGCATCATCCGCAGCGAGTTTTGCGCAAGGCGACAAGGACACAGAGGAAGGGGAATAT
CTTGGTCAGTTACCGCCTCTTACCTTAGGACAAGTTCTTCATTGTCACCAAGGTGAATTAGTCGAGAAGCAAACTCAACC
TCCCAAAGCCTTTACCGATGCTACCTTGCTGAGTGCGATGACGGGGATTAACCGTTATGTGACTGACCCAGAGATCCGCA
AAATTTTAAAGGAAACCGATGGCTTAGGTACGGAAGCGACTCGCGCAGGCATTATTGAACTGCTGTTTAAACGGGGCTTT
ATTCAGCGTCAGGGCAAGTCGATTGTGGCCACGGAGGTGGGCAAGGGCTTAATTAATAGTTTACCCCTTAGCGCCACGAC
ACCCGATATGACGGCGCTGTGGGAGGCAAGTCTAAACGGTATCTGCCATAAAGAAATCTCATATCAAGGCTTTATGCAAC
CACTGCTGGGGACGCTAAATAACTTAATTGAAAATGCGGGCGCTCAATTACCCTCAGCCCTCAATGGCTTAAAAGGGCAG
GGCTTTAAGCGTGCAGCAACGAAAAAGTCCGGCTATCGAAAAGCTGGAGCCAAAGGGGCAGGATGGAAAAAATCCGGCAC
TAAAAACGCGGCAGTGGGAAGCTCGGCCGCTAAAACTACCGCGGCTAAATCGACGGTCTCGACATCCGGTACGCCTACTA
CGCCTAGACGGCGAGCGAGCAAAGCGCCCGCCCAAGCTTGA

Upstream 100 bases:

>100_bases
AGAGCGAGTTAAATCGGCCCTTGAGGTAAAACGGCGGTTTGCACTCTTGTCCGCTTTGGGCTAAATTACACTGTAATTAT
ATCCAGTATTTAATGTAGAT

Downstream 100 bases:

>100_bases
TGACACAAATGTAATCAAACTGTCGCTAATGCTCGCGATCTAGTAACGATATTTAATATATTCCGCGATATTTAATTGCC
GCCAATCACGATATTTAGCG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDANGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQAFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHGLASGVLAAITGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDTEEGEY
LGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEVGKGLINSLPLSATTPDMTALWEASLNGICHKEISYQGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGAKGAGWKKSGTKNAAVGSSAAKTTAAKSTVSTSGTPTTPRRRASKAPAQA

Sequences:

>Translated_706_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDANGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQAFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHGLASGVLAAITGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDTEEGEY
LGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEVGKGLINSLPLSATTPDMTALWEASLNGICHKEISYQGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGAKGAGWKKSGTKNAAVGSSAAKTTAAKSTVSTSGTPTTPRRRASKAPAQA
>Mature_706_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDANGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQAFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHGLASGVLAAITGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDTEEGEY
LGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEVGKGLINSLPLSATTPDMTALWEASLNGICHKEISYQGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGAKGAGWKKSGTKNAAVGSSAAKTTAAKSTVSTSGTPTTPRRRASKAPAQA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=638, Percent_Identity=23.1974921630094, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788061, Length=677, Percent_Identity=55.6868537666174, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=551, Percent_Identity=24.3194192377495, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=647, Percent_Identity=23.8021638330757, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585251, Length=536, Percent_Identity=24.0671641791045, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77389; Mature: 77389

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDANGNCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPEKWQLKPKAATRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQAFTAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCCEEECC
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEECHHEEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPTEQHGLASGVLAAITGGAPELVA
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHE
HAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQRERQLYLHIARQYLAQFYPAY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDTEEGEY
HCCCCEEEEEECCCEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
LGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETD
HCCCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLGTEATRAGIIELLFKRGFIQRQGKSIVATEVGKGLINSLPLSATTPDMTALWEASLNG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ICHKEISYQGFMQPLLGTLNNLIENAGAQLPSALNGLKGQGFKRAATKKSGYRKAGAKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHCCCHHCCCCCC
GWKKSGTKNAAVGSSAAKTTAAKSTVSTSGTPTTPRRRASKAPAQA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDANGNCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPEKWQLKPKAATRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQAFTAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCCEEECC
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEECHHEEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPTEQHGLASGVLAAITGGAPELVA
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHE
HAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQRERQLYLHIARQYLAQFYPAY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDTEEGEY
HCCCCEEEEEECCCEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
LGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETD
HCCCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLGTEATRAGIIELLFKRGFIQRQGKSIVATEVGKGLINSLPLSATTPDMTALWEASLNG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ICHKEISYQGFMQPLLGTLNNLIENAGAQLPSALNGLKGQGFKRAATKKSGYRKAGAKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHCCCHHCCCCCC
GWKKSGTKNAAVGSSAAKTTAAKSTVSTSGTPTTPRRRASKAPAQA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]