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The map label for this gene is pcm [H]

Identifier: 113969465

GI number: 113969465

Start: 1315404

End: 1316039

Strand: Direct

Name: pcm [H]

Synonym: Shewmr4_1121

Alternate gene names: 113969465

Gene position: 1315404-1316039 (Clockwise)

Preceding gene: 113969464

Following gene: 113969466

Centisome position: 27.95

GC content: 51.26

Gene sequence:

>636_bases
ATGACTCGAGTTGCCTTAACATCGGCGGTGAATTTAGCTAAAAAGCTTCAGGAGGCGGGGATCCGTCATCCAGCCGTTCT
TAAGGCAATATCCCATACCCCGCGAGAACTGTTTCTTGATAATGCGCTGGCCCATAAAGCCTACGAAAATACCGCCTTGC
CCATAGGCCAAGGACAAACCATTTCGCAGCCGTATATTGTTGCACGTATGACTGAGTTACTGCTGCAACATCAGCCGCAA
AAGGTGCTTGAGGTGGGGACGGGCTCTGGCTACCAAGCGGCTATTCTCGCACAACTGGTGCCAGAACTGTGCACCATTGA
GCGTATTAAAGGTTTACAGATCCAAGCGAGGCAAAGATTAAAGCGACTCGATCTGCATAATGTGTCATTCAAATATGGCG
ATGGCTGGCAGGGCTGGCCGAATCGCAGCCCCTTTGATGGCATTATGGTCACGGCAGCAGCGGCCAAGGTTCCCGAGGCC
TTATTATCCCAGTTAGCCGAAGGCGGCGTGCTGATCATCCCTGTGGGTGAAGAGACGCAACAATTGATGCGCTTTACCCG
CCGCTCGGATCGTTTTAGTTCTGAAGTGATAGAAACCGTCAAATTTGTTCCCTTGGTCAATGGCGAACTCGCTTAA

Upstream 100 bases:

>100_bases
TCCATGCGATTGCCCATGGTTATGTGTCGATCACTCCCTTAACCGTGGACTTGACCGCGTATAGACAATTATCGGTATTG
CAAGATTGGGTAGATAAAAT

Downstream 100 bases:

>100_bases
TTTATTTGCTTAAGTTTTTCGCTTAGGTCACTTAGTTAAATACTTGACCTTTTATGATTAGGTAAAACAGTAAGATAGCC
CTAAAGAGGAGTTTTTATTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTRVALTSAVNLAKKLQEAGIRHPAVLKAISHTPRELFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLQHQPQ
KVLEVGTGSGYQAAILAQLVPELCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWQGWPNRSPFDGIMVTAAAAKVPEA
LLSQLAEGGVLIIPVGEETQQLMRFTRRSDRFSSEVIETVKFVPLVNGELA

Sequences:

>Translated_211_residues
MTRVALTSAVNLAKKLQEAGIRHPAVLKAISHTPRELFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLQHQPQ
KVLEVGTGSGYQAAILAQLVPELCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWQGWPNRSPFDGIMVTAAAAKVPEA
LLSQLAEGGVLIIPVGEETQQLMRFTRRSDRFSSEVIETVKFVPLVNGELA
>Mature_210_residues
TRVALTSAVNLAKKLQEAGIRHPAVLKAISHTPRELFLDNALAHKAYENTALPIGQGQTISQPYIVARMTELLLQHQPQK
VLEVGTGSGYQAAILAQLVPELCTIERIKGLQIQARQRLKRLDLHNVSFKYGDGWQGWPNRSPFDGIMVTAAAAKVPEAL
LSQLAEGGVLIIPVGEETQQLMRFTRRSDRFSSEVIETVKFVPLVNGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=193, Percent_Identity=34.1968911917098, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1789100, Length=200, Percent_Identity=61, Blast_Score=252, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71983477, Length=174, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=139, Percent_Identity=34.5323741007194, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=31.4814814814815, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23241; Mature: 23110

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVALTSAVNLAKKLQEAGIRHPAVLKAISHTPRELFLDNALAHKAYENTALPIGQGQT
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
ISQPYIVARMTELLLQHQPQKVLEVGTGSGYQAAILAQLVPELCTIERIKGLQIQARQRL
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRLDLHNVSFKYGDGWQGWPNRSPFDGIMVTAAAAKVPEALLSQLAEGGVLIIPVGEETQ
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
QLMRFTRRSDRFSSEVIETVKFVPLVNGELA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRVALTSAVNLAKKLQEAGIRHPAVLKAISHTPRELFLDNALAHKAYENTALPIGQGQT
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
ISQPYIVARMTELLLQHQPQKVLEVGTGSGYQAAILAQLVPELCTIERIKGLQIQARQRL
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KRLDLHNVSFKYGDGWQGWPNRSPFDGIMVTAAAAKVPEALLSQLAEGGVLIIPVGEETQ
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
QLMRFTRRSDRFSSEVIETVKFVPLVNGELA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]