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The map label for this gene is proB [H]

Identifier: 113969296

GI number: 113969296

Start: 1114486

End: 1115604

Strand: Direct

Name: proB [H]

Synonym: Shewmr4_0952

Alternate gene names: 113969296

Gene position: 1114486-1115604 (Clockwise)

Preceding gene: 113969295

Following gene: 113969297

Centisome position: 23.68

GC content: 50.67

Gene sequence:

>1119_bases
ATGAATTTAAGTGAGATAGCGTATCGCCGCGTGGTAGTGAAGTTAGGGACGAGTGTGCTGACTTCTGGCAGTAAACAGCT
GGATAAAGCCCATATGGTTGAGCTTGCTCGTCAAATGGCCGCACTGATGAGATCAGGGGTCGAAGTCGTATTATGCACTT
CTGGCGCAATTGCCGCCGGACGCGAACATCTCCAATACCCGGTATTGCCCGACACTATGGCTAACAAACAGTTATTAGCC
GCTGTCGGGCAGAGTCAGCTTATTCTCGCTTGGGCACAACTCTTTAGTATCTATGGTTTGCATGTGGGGCAATTGCTGCT
AACCCGTGCCGATCTACACGATAGAGAGCGCTATTTAAATGCCCGCGATACGCTTAATGCCTTGCTGGCAAACAATATTA
TCCCCATCATCAACGAAAACGATGCCGTCGCTACTAATGAGATCAAAGTGGGCGACAACGATAACTTGTCGGCGCGTGCT
GCACTGCTGTGTGATGCCGACCTGTTGATTTTGTTGACGGATCAAAAGGGCTTATTCGATGCCGATCCGCGCACTAACCC
GAATGCTAAGCTGATTTCTCAGGTCGAGAAAATTGATGACAGTCTGCGTCTGTTAGCGGGTGGTTCGGTGTCGGGCTTAG
GTACGGGCGGAATGTCGACTAAGCTAGAAGCTGCGGATATTGCTCGCCGTGCGGGCATTGAAGTGGTGATTGCCTCGGGT
CATCACCCCGATGTGATTAAAAAAGTCGTCGGCAAAGAATCTGTCGGCACGCATTTTAGTGCCATCGAAAATCCGTTAGA
GAGCCGCAAACAGTGGATTTTAGCGGGACCCGCCGCTCAAGGTTCATTGGTGCTCGATGCGGGCGCGGTGAAAGCCGTGA
CCGAAAAAGGTCGCAGCCTGCTCTCTAAGGGGATTATTGGCGTAAAAGGTGAATTTGAACGTGGTGCAACCCTGCAATTG
GTTGACCAAAAAGGCAAAATCATTGCCAGAGGCATCACCCGTTACTGTGGTGAGGCCTTAGGGGTAATTGCGGGTAAACA
TTCCGACGAAATCGAGTCAGTGCTCGGTTACGATTATGGTGACGCAATAGTGCACCGCAACGACATGGTCGTCTTATAA

Upstream 100 bases:

>100_bases
AAGCAGAATTGAAAATTTCTACCAACATCCTGCCCACTAACTACCAATTGCTCTGCGGATTAGGTATAAGGATTATCCGG
TTATTTTCTACATATGAATA

Downstream 100 bases:

>100_bases
GCGAGGTCTGTTTTGAGTCAAACTAATCAAGCGCAATATTTACAACAACTGGGCAGTCAGGCTAAACAAGCCAGCTATGC
CTTAGCAAATCTTAGCGCCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMRSGVEVVLCTSGAIAAGREHLQYPVLPDTMANKQLLA
AVGQSQLILAWAQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLANNIIPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVEKIDDSLRLLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVGKESVGTHFSAIENPLESRKQWILAGPAAQGSLVLDAGAVKAVTEKGRSLLSKGIIGVKGEFERGATLQL
VDQKGKIIARGITRYCGEALGVIAGKHSDEIESVLGYDYGDAIVHRNDMVVL

Sequences:

>Translated_372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMRSGVEVVLCTSGAIAAGREHLQYPVLPDTMANKQLLA
AVGQSQLILAWAQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLANNIIPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVEKIDDSLRLLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVGKESVGTHFSAIENPLESRKQWILAGPAAQGSLVLDAGAVKAVTEKGRSLLSKGIIGVKGEFERGATLQL
VDQKGKIIARGITRYCGEALGVIAGKHSDEIESVLGYDYGDAIVHRNDMVVL
>Mature_372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMRSGVEVVLCTSGAIAAGREHLQYPVLPDTMANKQLLA
AVGQSQLILAWAQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLANNIIPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVEKIDDSLRLLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVGKESVGTHFSAIENPLESRKQWILAGPAAQGSLVLDAGAVKAVTEKGRSLLSKGIIGVKGEFERGATLQL
VDQKGKIIARGITRYCGEALGVIAGKHSDEIESVLGYDYGDAIVHRNDMVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=330, Percent_Identity=34.5454545454545, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI62912457, Length=328, Percent_Identity=34.4512195121951, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786437, Length=361, Percent_Identity=63.4349030470914, Blast_Score=453, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.4569288389513, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.4569288389513, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=36.144578313253, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=34.7931873479319, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=35.5172413793103, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39673; Mature: 39673

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMRSGVEVVLCTSGAIAAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHC
REHLQYPVLPDTMANKQLLAAVGQSQLILAWAQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ARDTLNALLANNIIPIINENDAVATNEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRTNPNAKLISQVEKIDDSLRLLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
CCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
HHPDVIKKVVGKESVGTHFSAIENPLESRKQWILAGPAAQGSLVLDAGAVKAVTEKGRSL
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHH
LSKGIIGVKGEFERGATLQLVDQKGKIIARGITRYCGEALGVIAGKHSDEIESVLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
DAIVHRNDMVVL
CCEEECCCEEEC
>Mature Secondary Structure
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMRSGVEVVLCTSGAIAAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHC
REHLQYPVLPDTMANKQLLAAVGQSQLILAWAQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ARDTLNALLANNIIPIINENDAVATNEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRTNPNAKLISQVEKIDDSLRLLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
CCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
HHPDVIKKVVGKESVGTHFSAIENPLESRKQWILAGPAAQGSLVLDAGAVKAVTEKGRSL
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHH
LSKGIIGVKGEFERGATLQLVDQKGKIIARGITRYCGEALGVIAGKHSDEIESVLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
DAIVHRNDMVVL
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]