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The map label for this gene is epsE [H]

Identifier: 113968502

GI number: 113968502

Start: 179790

End: 181355

Strand: Direct

Name: epsE [H]

Synonym: Shewmr4_0157

Alternate gene names: 113968502

Gene position: 179790-181355 (Clockwise)

Preceding gene: 113968501

Following gene: 113968503

Centisome position: 3.82

GC content: 52.3

Gene sequence:

>1566_bases
ATGAGTGAAATACAAGTAACCCAAGTCGAGGATTTAAGCCTAGTTTCTAACGAACTAGGGCTTGAAGCCGAAGGTGATGA
GGTATTTCGCTCCAGCAGCAAAGAGCGTTTACCCTTTGCCTTTGCCCACCGCCATGAGGTGATTTTAGCCTTTGGCGAGG
ATGGTGCCTTGAGCCTGTTTTATACCGCCAAAACCCCGTTAACGGCCATGCTCGAAGCGCGCCGTTACTCGGGTGTGGAT
TTGCCGCTGGTATTGCTCGAACCGGGCAAGTTTGAGGCGAAATTAACTCAGGCCTATCAAGCCAACTCCTCCGAAGCGCA
GCAGCTGATGGAAGATATTGGCAACGAGATGGATTTATTCACCCTCGCCGAAGAGCTGCCGCAGACCGAAGATCTGCTCG
AAGGCGATGATGATGCGCCTATTATCAAGCTGATTAACGCCTTGTTATCTGAAGCGATTAAAGAAGAAGCCTCGGATATC
CATATCGAAACCTACGAGAAGCAGTTGGTCGTGCGTTTCCGTATCGACGGTGTGCTGAAGGAAGTGCTTAAGCCGAATCG
TAAGTTGTCATCCTTGTTGGTGTCGCGGATCAAGGTGATGGCGCGTCTCGATATCGCCGAGAAACGTGTGCCACAAGACG
GCCGTATTTCGCTGCGGATTGCGGGTCGTGCGGTGGATGTGCGGGTATCGACCATGCCATCGAGCCATGGCGAGCGTGTG
GTGCTGCGTCTGTTAGATAAAAACGCCGGTAATCTGGATTTAAAACAATTGGGTATGACCGACAGTATCCGTGCCAAGTT
CGACGAGCTTATTCGTCGTCCCCACGGCATTATTCTGGTCACAGGCCCTACGGGTTCCGGTAAGAGTACTACGCTGTACG
CCGGGCTTACCGAAATTAACTCCAAAGATACCAACATCTTAACCGTTGAAGATCCGATCGAATACGAGTTAGAAGGCATA
GGTCAAACCCAAGTGAACACTAAGGCTGACATGACCTTCGCCCGTGGTCTGCGTGCGATTCTGCGTCAGGACCCGGATGT
GGTGATGATCGGCGAAATCCGTGACCTAGAAACCGCCCAAATTGCGGTGCAGGCATCCTTGACCGGTCACTTAGTGATTT
CAACCCTGCATACCAACACGGCTTCTGGTGCAATTACCCGTTTGCAAGACATGGGCGTAGAGCCATTCCTAGTCTCATCG
AGTTTGCTTGGGGTATTAGCCCAGCGTTTAATTCGTACTCTCTGTCCAAAATGTAAGACAGAGCATGTGCCCGATGCCCG
TGAGCGTGAGCTGCTGGGAATGGCGGCAGACGATCATCGTCATATTTATCGCGCCAATGGCTGTAAGGCCTGTGGTAGCA
GTGGCTACCGTGGTCGTACGGGTATCCATGAGCTATTGCTGGTCGATGATAATGTGCGCGAGCTGATCCACGGTGGACGT
GGCGAACTGGCGATTGAGAAATATATCCGCCAGTCGGTGCCAAGCATTCGCCACGATGGCATGAGCAAGGTGCTCTCAGG
TATCACCACTCTGGAAGAAGTCCTGAGGGTGACCCGCGAGGAGTAA

Upstream 100 bases:

>100_bases
TAGATCGTTACAAAGAAGGCAAAGGGTTAGACACCCAGATGCGTGAAACGGACTCGACCCTCAAGTCGCTGGATGAAAAC
AAGAACAAAGATAAAACACA

Downstream 100 bases:

>100_bases
GCTATGCCAGCATTTGAATATAAGGCGCTGGACGCCAAGGGAAAACAGCTAAAAGGGGTGATTGAGGCGGATACCGCCAG
ACATGCTCGTAGTCAGCTGC

Product: type II secretion system protein E (GspE)

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAFGEDGALSLFYTAKTPLTAMLEARRYSGVD
LPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLKQLGMTDSIRAKFDELIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKTEHVPDARERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE

Sequences:

>Translated_521_residues
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAFGEDGALSLFYTAKTPLTAMLEARRYSGVD
LPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLKQLGMTDSIRAKFDELIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKTEHVPDARERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
>Mature_520_residues
SEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAFGEDGALSLFYTAKTPLTAMLEARRYSGVDL
PLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIH
IETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERVV
LRLLDKNAGNLDLKQLGMTDSIRAKFDELIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGIG
QTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSS
LLGVLAQRLIRTLCPKCKTEHVPDARERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGRG
ELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=57.8288100208768, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=43.75, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI87082188, Length=245, Percent_Identity=37.1428571428571, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57555; Mature: 57424

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAFGEDGALSLF
CCCCEEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEE
YTAKTPLTAMLEARRYSGVDLPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLKQLGMTDSIRAKFDELIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELEGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKT
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDARERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAFGEDGALSLF
CCCEEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEE
YTAKTPLTAMLEARRYSGVDLPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLKQLGMTDSIRAKFDELIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELEGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKT
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDARERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]