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The map label for this gene is epsD [H]

Identifier: 113968501

GI number: 113968501

Start: 177691

End: 179793

Strand: Direct

Name: epsD [H]

Synonym: Shewmr4_0156

Alternate gene names: 113968501

Gene position: 177691-179793 (Clockwise)

Preceding gene: 113968500

Following gene: 113968502

Centisome position: 3.78

GC content: 48.74

Gene sequence:

>2103_bases
ATGAATAACAAACGGATTCGACGCAAACTGATTGCTGGAGTTGTGGCTGGCGCCACTATGCTGACCTCGCAATTTGTTTG
GTCTGAACAATATGCGGCCAACTTTAAAGGCACTGATATCCAAGAGTTTATCAACATTGTTGGTAAAAACCTGAATAAAA
CCATCATTGTTGACCCGACCATTCGCGGCAAGATCAACGTGCGCAGTTATGATCTGCTCAACGATGAGCAGTATTACCAA
TTCTTCCTCAACGTGCTGCAAGTGTACGGTTATGCCATCGTCGAGATGGAAAACAACGTCATTAAAGTCATTAAAGACAA
AGATGCTAAAACCGCGGCGATTCGTGTTGCTAACGACAACGACCCAGGTTTAGGTGATGAAATGGTCACCCGCATCGTGG
CGCTGTACAACACCGAAGCCAAGCAATTAGCACCGTTATTACGTCAGTTAAACGATAACGCCGGTGGCGGTAACGTAGTG
AACTACGACCCATCGAACGTACTGATGTTGTCAGGCCGCGCCGCTGTGGTGAACAAGTTAGTTGAAATCGTTCGCCGTGT
AGATAAGCAGGGCGATACCTCGGTACAAGTGGTGCCGCTCGAATACGCCTCAGCCGGTGAAATGGTGCGAATTATCGATA
CTCTTTACCGCGCGACAGCTAACCAAGCGCAGCTCCCAGGACAAGCGCCCAAGGTCGTTGCCGACGAGCGCATTAACGCC
GTAGTGGTCAGTGGTGACGAAAAGAGCCGCCAACGCGTGGTTGAGCTTATCCATCGCCTCGATGCCGAGCAGGCCAGCAC
GGGCAATACTAAGGTACGTTATTTACGTTATGCCAAGGCTGAAGATTTAGTCGAGGTGCTGACCGGATTTGCGCAAAAGC
TTGAGGGTGAAAAAGATCCAAGCGCCCAGGCTGGCGGTAAACGTCGTAATGAAATCAATATTATGGCCCACACGGATACT
AACGCCTTAGTGATCAGCGCCGAGCCAGATCAAATGCGCACTATCGAGAGTGTGATTAACCAACTCGATATTCGCCGCGC
CCAAGTATTAGTCGAAGCCATTATCGTGGAAGTCGCCGAAGGCGATAACGTCGGTTTTGGTGTGCAATGGGCCGCTAAAG
CGGGCGGTGGTACTCAGTTCAATAACTTAGGCCCAACCATTGGTGAAATTGGCGCCGGTATTTGGCAGGCACAGGGAGAA
GATGGGACAACTGTTTGTACCGAGAATGGCACTTGTACTGAAAACCCAGATTCTAGAGGTGATGTGACTTTACTTGCCCA
AGCCTTAGGTAAAGTGAATGGTATGGCTTGGGGCGTGGCCATGGGCGACTTTGGCGCCTTAATACAAGCAGTATCGGCCG
ATACCAACTCAAACGTACTGGCGACACCTTCGATTACAACTCTAGATAACCAAGAAGCCTCGTTTATCGTCGGTGACGAA
GTGCCGATTCTGACCGGTTCTACCGCCAGCTCAAACAACAGTAACCCATTCCAAACCGTTGAACGTAAAGAGGTTGGGGT
GAAGTTAAAAGTTGTGCCTCAGATCAACGAAGGCAACGCGGTTAAGCTCGCCATTGAGCAAGAAGTCTCGGGTGTAAACG
GTAATACGGGTGTAGATATCTCCTTCGCGACTCGCCGTTTAACCACCACTGTGATGGCTGACTCAGGGCAAATCGTGGTA
CTCGGTGGTTTGATTAACGAAGAAGTCCAAGAAAGTGTCCAGAAAGTACCATTCCTCGGTGATATTCCGGTACTCGGACA
CTTATTCAAATCTTCTTCCAGTAAGAAGACCAAAAAGAACCTGATGATCTTCATCAAGCCCACCATTATCCGTGATGGTG
TGACTATGGAAGGCATCGCAGGCCGTAAATACAACTATTTCAGAGCATTACAACTTGAGCAGCAAGAGCGCGGCGTGAAC
CTAATGCCAAATACTCAAGTGCCAATTCTGGAAGAATGGAATCAATCTGAATATTTGCCGCCCGAAGTGAACGACATTTT
AGATCGTTACAAAGAAGGCAAAGGGTTAGACACCCAGATGCGTGAAACGGACTCGACCCTCAAGTCGCTGGATGAAAACA
AGAACAAAGATAAAACACAATGA

Upstream 100 bases:

>100_bases
TGAATTAACTGAAGTTTCCATCATGGTTGAGCGGGAAGGTCAATTGGTTGAAATCATGTTTAGTTTGCCGCAATAACACG
CGTTAGAGGAAAGTAAAATA

Downstream 100 bases:

>100_bases
GTGAAATACAAGTAACCCAAGTCGAGGATTTAAGCCTAGTTTCTAACGAACTAGGGCTTGAAGCCGAAGGTGATGAGGTA
TTTCGCTCCAGCAGCAAAGA

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQGE
DGTTVCTENGTCTENPDSRGDVTLLAQALGKVNGMAWGVAMGDFGALIQAVSADTNSNVLATPSITTLDNQEASFIVGDE
VPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQIVV
LGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLDTQMRETDSTLKSLDENKNKDKTQ

Sequences:

>Translated_700_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQGE
DGTTVCTENGTCTENPDSRGDVTLLAQALGKVNGMAWGVAMGDFGALIQAVSADTNSNVLATPSITTLDNQEASFIVGDE
VPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQIVV
LGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLDTQMRETDSTLKSLDENKNKDKTQ
>Mature_700_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQGE
DGTTVCTENGTCTENPDSRGDVTLLAQALGKVNGMAWGVAMGDFGALIQAVSADTNSNVLATPSITTLDNQEASFIVGDE
VPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQIVV
LGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLDTQMRETDSTLKSLDENKNKDKTQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=663, Percent_Identity=43.7405731523379, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789793, Length=367, Percent_Identity=25.6130790190736, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 76356; Mature: 76356

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
IRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDN
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
DPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHH
VEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCE
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDP
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
SAQAGGKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDNVGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQGEDGTTVCTENGTCTENPDSRG
CCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCCC
DVTLLAQALGKVNGMAWGVAMGDFGALIQAVSADTNSNVLATPSITTLDNQEASFIVGDE
CHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCEEEECCC
VPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDI
CEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEE
SFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKN
EEHHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVNLMPNTQVPILEEWNQSEYLP
EEEEEECEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNDILDRYKEGKGLDTQMRETDSTLKSLDENKNKDKTQ
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
IRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDN
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
DPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHH
VEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCE
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDP
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
SAQAGGKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDNVGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQGEDGTTVCTENGTCTENPDSRG
CCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCCC
DVTLLAQALGKVNGMAWGVAMGDFGALIQAVSADTNSNVLATPSITTLDNQEASFIVGDE
CHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCEEEECCC
VPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDI
CEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEE
SFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKN
EEHHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVNLMPNTQVPILEEWNQSEYLP
EEEEEECEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNDILDRYKEGKGLDTQMRETDSTLKSLDENKNKDKTQ
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]