Click here to switch to the map view.

The map label for this gene is ytcB [H]

Identifier: 113475902

GI number: 113475902

Start: 3519633

End: 3520676

Strand: Direct

Name: ytcB [H]

Synonym: Tery_2262

Alternate gene names: 113475902

Gene position: 3519633-3520676 (Clockwise)

Preceding gene: 113475901

Following gene: 113475903

Centisome position: 45.41

GC content: 38.22

Gene sequence:

>1044_bases
ATGTCTTGCTTGGCTGGAAAAAAAATGTTGATAACTGGAGGTGCAGGTTTTTTAGGTTATTACTTGGTTAAGTCAGCTTT
ATTCTGGAATCAGTCTGTAGAAAAGTCTCGAAAAATAGATTTGACAGTTTATGACAATTATATCCGTGGCGTACCTAGTT
GGTTAACCGATTTAGAAGAAAACAGTTTTAGCTTGGTAAAGCACGATATTACTCAACCACTGCCTAGTAATATTGATGAT
TTTCAGTACATAATTCATGCAGCTTCCATAGCTTCTCCGACATACTATCGTAAGTATCCTATTGAAACGATGGATGCTAA
TGTTAATGGGTTAAGGTTTTTGTTGGAATATTGCCAACAACAAAAACATAAACCTCAACCAGTGGAAGGTTTCCTATTTT
ATTCGACTAGCGAAATTTACGGCGATCCGACTCCTGAAAATATCCCTACTCCAGAAACCTACAGAGGAAATGTTTCTAGC
ACTGGTCCGAGGGCTTGTTATGATGAATCTAAACGCTATGGTGAAACTCTGTGCGTGAATTTTGCCCAGCAGTACAATTT
GCCGATTAAAATAGCACGTCCTTTTAATAATTATGGCCCTGGGTTAAAAATTACTGATAAAAGGGTAATTCCTGATTTCG
CTCGCGATATCCTGGCGGGTAAGGATATTGTCATGCTTTCTGATGGTTCCCCCACTAGGACTTTTTGTTATGTCGCCGAT
GCAATTATTGGCTATTACAAGATTTTAACTAAGGGTAAGCAAGGTGAAGCTTACAATATCGGTGTGGAAAAACCAGAGAT
TTCTATGCTTGATTTGGCAAATAAGATTGTGGAGTTAGCAGGAGATTTATTCAATTATTCTGGTAAAGTTGTTCGTCAAG
CTAGTGCTGACCAAAATTACTTGGTTGATAATCCTAATCGTCGTTGTCCTGTCATTGCTAAAGCTAGAAATGATTTGGGA
TACAATCCTGGTATTTCTCTTGATGAAGGGCTAAAAAGATCGCTGATATGGTATCAAGATAATTCAGAAGCAGCGGAGGC
ATAA

Upstream 100 bases:

>100_bases
TTTGTCATAAATTTTCAACATAATAGAAATAATGGTAAATACTATCCAAACAGTTGTCGAGAAAGATTTAGAGTACATCT
GCACTCTTCTTAAGGAAGAG

Downstream 100 bases:

>100_bases
ATGAAAGTTTCTATGATTGGTACGGGATATGTAGGTCTAGTGTCTGGGGTTTGCTTTGCCGAAAAGGGGCATTCGGTTAT
TTGTGTAGATATTGACCAAG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSCLAGKKMLITGGAGFLGYYLVKSALFWNQSVEKSRKIDLTVYDNYIRGVPSWLTDLEENSFSLVKHDITQPLPSNIDD
FQYIIHAASIASPTYYRKYPIETMDANVNGLRFLLEYCQQQKHKPQPVEGFLFYSTSEIYGDPTPENIPTPETYRGNVSS
TGPRACYDESKRYGETLCVNFAQQYNLPIKIARPFNNYGPGLKITDKRVIPDFARDILAGKDIVMLSDGSPTRTFCYVAD
AIIGYYKILTKGKQGEAYNIGVEKPEISMLDLANKIVELAGDLFNYSGKVVRQASADQNYLVDNPNRRCPVIAKARNDLG
YNPGISLDEGLKRSLIWYQDNSEAAEA

Sequences:

>Translated_347_residues
MSCLAGKKMLITGGAGFLGYYLVKSALFWNQSVEKSRKIDLTVYDNYIRGVPSWLTDLEENSFSLVKHDITQPLPSNIDD
FQYIIHAASIASPTYYRKYPIETMDANVNGLRFLLEYCQQQKHKPQPVEGFLFYSTSEIYGDPTPENIPTPETYRGNVSS
TGPRACYDESKRYGETLCVNFAQQYNLPIKIARPFNNYGPGLKITDKRVIPDFARDILAGKDIVMLSDGSPTRTFCYVAD
AIIGYYKILTKGKQGEAYNIGVEKPEISMLDLANKIVELAGDLFNYSGKVVRQASADQNYLVDNPNRRCPVIAKARNDLG
YNPGISLDEGLKRSLIWYQDNSEAAEA
>Mature_346_residues
SCLAGKKMLITGGAGFLGYYLVKSALFWNQSVEKSRKIDLTVYDNYIRGVPSWLTDLEENSFSLVKHDITQPLPSNIDDF
QYIIHAASIASPTYYRKYPIETMDANVNGLRFLLEYCQQQKHKPQPVEGFLFYSTSEIYGDPTPENIPTPETYRGNVSST
GPRACYDESKRYGETLCVNFAQQYNLPIKIARPFNNYGPGLKITDKRVIPDFARDILAGKDIVMLSDGSPTRTFCYVADA
IIGYYKILTKGKQGEAYNIGVEKPEISMLDLANKIVELAGDLFNYSGKVVRQASADQNYLVDNPNRRCPVIAKARNDLGY
NPGISLDEGLKRSLIWYQDNSEAAEA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=34.3108504398827, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=25.4385964912281, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=27.0893371757925, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=25.9259259259259, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788589, Length=357, Percent_Identity=22.4089635854342, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=32.9376854599407, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=351, Percent_Identity=26.7806267806268, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=35.5029585798817, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 38983; Mature: 38851

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCLAGKKMLITGGAGFLGYYLVKSALFWNQSVEKSRKIDLTVYDNYIRGVPSWLTDLEE
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCHHHHHHCC
NSFSLVKHDITQPLPSNIDDFQYIIHAASIASPTYYRKYPIETMDANVNGLRFLLEYCQQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHCCCCHHHHHHHHHHHHH
QKHKPQPVEGFLFYSTSEIYGDPTPENIPTPETYRGNVSSTGPRACYDESKRYGETLCVN
HCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAQQYNLPIKIARPFNNYGPGLKITDKRVIPDFARDILAGKDIVMLSDGSPTRTFCYVAD
HHHHCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
AIIGYYKILTKGKQGEAYNIGVEKPEISMLDLANKIVELAGDLFNYSGKVVRQASADQNY
HHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
LVDNPNRRCPVIAKARNDLGYNPGISLDEGLKRSLIWYQDNSEAAEA
EECCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEECCCHHCCC
>Mature Secondary Structure 
SCLAGKKMLITGGAGFLGYYLVKSALFWNQSVEKSRKIDLTVYDNYIRGVPSWLTDLEE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCHHHHHHCC
NSFSLVKHDITQPLPSNIDDFQYIIHAASIASPTYYRKYPIETMDANVNGLRFLLEYCQQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHCCCCHHHHHHHHHHHHH
QKHKPQPVEGFLFYSTSEIYGDPTPENIPTPETYRGNVSSTGPRACYDESKRYGETLCVN
HCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAQQYNLPIKIARPFNNYGPGLKITDKRVIPDFARDILAGKDIVMLSDGSPTRTFCYVAD
HHHHCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
AIIGYYKILTKGKQGEAYNIGVEKPEISMLDLANKIVELAGDLFNYSGKVVRQASADQNY
HHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
LVDNPNRRCPVIAKARNDLGYNPGISLDEGLKRSLIWYQDNSEAAEA
EECCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]