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The map label for this gene is hisF [H]

Identifier: 113475438

GI number: 113475438

Start: 2688586

End: 2689356

Strand: Direct

Name: hisF [H]

Synonym: Tery_1766

Alternate gene names: 113475438

Gene position: 2688586-2689356 (Clockwise)

Preceding gene: 113475436

Following gene: 113475439

Centisome position: 34.69

GC content: 42.54

Gene sequence:

>771_bases
ATGTTGGCCAAAAGAATTTTACCTTGTCTTGATGTTAATGCAGGTCGAGTGGTCAAAGGAGTTAACTTCGTGAATCTTCA
AGATGCTGGAGACCCTGTGGAACTAGCCCAAGTATATAATCACGCTGGTGCTGATGAGTTAGTATTTCTAGATATTACGG
CCACTCACGAAGACCGAGATACAATCATAGATGTGGTTTATCGAACTGCAGAGCAAGTTTTTATTCCTTTGACAGTAGGA
GGTGGTATTCAATCCTTAGAAAACATTAAAAATTTGTTAAGAGCAGGTGCAGACAAAGTTAGTATAAATTCAGCAGCAGT
ACGTAACCCTAACCTAATTAATCAGGCCAGTGACCGTTTTGGAAATCAATGTATTGTAGTAGCTATTGATGCTTGTCGTC
GTTGTGAACCAGACAACCTAGGATGGGATGTCTATGTGCGAGGTGGCCGCGAAAATACTGGCAAGGATGCGATCGCTTGG
GCAATAGAAGTAGAAAAAAGAGGAGCAGGAGAAGTCCTCCTCACAAGTATGGATGCTGATGGAACCCAAGCAGGCTATGA
CCTAGAGCTTACAAGAACTATAGCAGAAAGTGTTCAGATTCCAGTAATTGCCTCTGGGGGGGCAGGTAACTGCCAACATA
TCTTTCAGGCTTTAACTGAAGGTAAAGCAGAAGCAGCATTGCTGGCTTCTTTACTACATTATGGTCAACTAAGTATAGCA
GAAATTAAGGATTATCTGGCGGGCCATGAAGTCCCTGTAAGACAAAGTTGA

Upstream 100 bases:

>100_bases
AATAATCTCCCATAAAAACCAATTAGAATTAACTTGATCCATTAACCTCTACTCTAAAATCGGTAATAATATACTCTGAA
TTCTAAAGTAATTGATTTAT

Downstream 100 bases:

>100_bases
TTTGAGTTGACTTGTAACTGTATATTCAAGATCTTAATATTTCTTGATATTTAGAAGCATTTTTAATAAATATTGTTAGA
ATAAGAGAATTGTTACAAAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLAKRILPCLDVNAGRVVKGVNFVNLQDAGDPVELAQVYNHAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENIKNLLRAGADKVSINSAAVRNPNLINQASDRFGNQCIVVAIDACRRCEPDNLGWDVYVRGGRENTGKDAIAW
AIEVEKRGAGEVLLTSMDADGTQAGYDLELTRTIAESVQIPVIASGGAGNCQHIFQALTEGKAEAALLASLLHYGQLSIA
EIKDYLAGHEVPVRQS

Sequences:

>Translated_256_residues
MLAKRILPCLDVNAGRVVKGVNFVNLQDAGDPVELAQVYNHAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENIKNLLRAGADKVSINSAAVRNPNLINQASDRFGNQCIVVAIDACRRCEPDNLGWDVYVRGGRENTGKDAIAW
AIEVEKRGAGEVLLTSMDADGTQAGYDLELTRTIAESVQIPVIASGGAGNCQHIFQALTEGKAEAALLASLLHYGQLSIA
EIKDYLAGHEVPVRQS
>Mature_256_residues
MLAKRILPCLDVNAGRVVKGVNFVNLQDAGDPVELAQVYNHAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENIKNLLRAGADKVSINSAAVRNPNLINQASDRFGNQCIVVAIDACRRCEPDNLGWDVYVRGGRENTGKDAIAW
AIEVEKRGAGEVLLTSMDADGTQAGYDLELTRTIAESVQIPVIASGGAGNCQHIFQALTEGKAEAALLASLLHYGQLSIA
EIKDYLAGHEVPVRQS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.8992248062016, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=29.5833333333333, Blast_Score=81, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=33.6507936507937, Blast_Score=149, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27477; Mature: 27477

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRILPCLDVNAGRVVKGVNFVNLQDAGDPVELAQVYNHAGADELVFLDITATHEDRD
CCHHHCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TIIDVVYRTAEQVFIPLTVGGGIQSLENIKNLLRAGADKVSINSAAVRNPNLINQASDRF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
GNQCIVVAIDACRRCEPDNLGWDVYVRGGRENTGKDAIAWAIEVEKRGAGEVLLTSMDAD
CCEEEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCCC
GTQAGYDLELTRTIAESVQIPVIASGGAGNCQHIFQALTEGKAEAALLASLLHYGQLSIA
CCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
EIKDYLAGHEVPVRQS
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLAKRILPCLDVNAGRVVKGVNFVNLQDAGDPVELAQVYNHAGADELVFLDITATHEDRD
CCHHHCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TIIDVVYRTAEQVFIPLTVGGGIQSLENIKNLLRAGADKVSINSAAVRNPNLINQASDRF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
GNQCIVVAIDACRRCEPDNLGWDVYVRGGRENTGKDAIAWAIEVEKRGAGEVLLTSMDAD
CCEEEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCCC
GTQAGYDLELTRTIAESVQIPVIASGGAGNCQHIFQALTEGKAEAALLASLLHYGQLSIA
CCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
EIKDYLAGHEVPVRQS
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]