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The map label for this gene is dus1 [H]

Identifier: 113475058

GI number: 113475058

Start: 2022108

End: 2023112

Strand: Reverse

Name: dus1 [H]

Synonym: Tery_1325

Alternate gene names: 113475058

Gene position: 2023112-2022108 (Counterclockwise)

Preceding gene: 113475059

Following gene: 113475055

Centisome position: 26.1

GC content: 37.41

Gene sequence:

>1005_bases
ATGACTATTCTATCTCCTGAATTAAAGAAAAAATTATCTACTCCACTAAAAATTGGCAACTTTGAAGTTAAAAGTCGTGT
CCTTCAATCACCATTATCAGGAGTAACAGACTTAGTATTTCGTCGTCTAGTACGTCGTCATGCTCCAGAGTCAATGATGT
ACACTGAAATGGTAAATGCTACAGGGTTACATTATGTAAAAGAATTACCTCAAATAATGGAGGTTGATAATAATGAGCGA
CCAATAAGTATTCAATTATTTGATTGTCGTCCAGATTTCTTAGCAGAAGCAGCAGAAATGGCTGTAAAAGAAGGAGCTGA
TACTGTTGATATTAATATGGGTTGTCCTGTTAATAAAATCACAAAAAACGGCGGGGGTTCTTCATTATTAAGACAACCAG
AATTAGCTGGAGAAATTGTTAGTAAAGTTGTTAAAGCTGTTCCTATTCCTGTTACTGTAAAAACAAGAATTGGTTGGAGT
AATCAAGAAATTAATATTCTCGAATTTGCTCAAAGAATGGAAGATGCAGGTGCCCAAATGTTAACTTTACATGGTCGGAC
TCGTGCCCAAGGATATAATGGCCCGGCCAGATGGGAATGGATTACAAAAGTTAAGGAAATACTTTCTATCCCAGTTATTG
CTAATGGAGATATTTTCTCTGTTGATGCTGCAATACGTTGTTTGGAGGAAACTAGAGCAGATGGAGTAATGTGCTCCCGT
GGAACTTTAGGTTATCCTTTTTTAGTGGGAGAAATTGATTATTTTTTGAAAAATGGTGTAAAAAAAGTATCACCAACTGC
AGCAGAAAAACTGGAGTGTGCTAAAGAGCATTTACGGGCACTATGGGAATATAAAGGCGATCGCGGGATTCGTCAAGCTC
GTAAGCATTTGACTTGGTATTCTAAGGGTTTTCCAGGAGCAGGTGAGTTGCGGGGTCAGTTGGCAAAAGTACAAACTGTA
GCGCAAGGTTGTGATTTGATTGATAAGGTAATTGAAAATTTATAG

Upstream 100 bases:

>100_bases
GGATAAAAAAAACGCATTACGTATTTTCTTGCCTCCTAATTTATTCAGAGGTACTGATAACTGATAAGTGATAAGTAATA
ACTGATAAGTAATAACTGAA

Downstream 100 bases:

>100_bases
TAGATAGTTTTGTGGCATGGATATCTTGCTTGTTCTTGATCATAAACCTGTTTTGTTATCAAAAAAGTTTGTAGGAGAGC
TTGAATTGTATCTATCTTAG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTILSPELKKKLSTPLKIGNFEVKSRVLQSPLSGVTDLVFRRLVRRHAPESMMYTEMVNATGLHYVKELPQIMEVDNNER
PISIQLFDCRPDFLAEAAEMAVKEGADTVDINMGCPVNKITKNGGGSSLLRQPELAGEIVSKVVKAVPIPVTVKTRIGWS
NQEINILEFAQRMEDAGAQMLTLHGRTRAQGYNGPARWEWITKVKEILSIPVIANGDIFSVDAAIRCLEETRADGVMCSR
GTLGYPFLVGEIDYFLKNGVKKVSPTAAEKLECAKEHLRALWEYKGDRGIRQARKHLTWYSKGFPGAGELRGQLAKVQTV
AQGCDLIDKVIENL

Sequences:

>Translated_334_residues
MTILSPELKKKLSTPLKIGNFEVKSRVLQSPLSGVTDLVFRRLVRRHAPESMMYTEMVNATGLHYVKELPQIMEVDNNER
PISIQLFDCRPDFLAEAAEMAVKEGADTVDINMGCPVNKITKNGGGSSLLRQPELAGEIVSKVVKAVPIPVTVKTRIGWS
NQEINILEFAQRMEDAGAQMLTLHGRTRAQGYNGPARWEWITKVKEILSIPVIANGDIFSVDAAIRCLEETRADGVMCSR
GTLGYPFLVGEIDYFLKNGVKKVSPTAAEKLECAKEHLRALWEYKGDRGIRQARKHLTWYSKGFPGAGELRGQLAKVQTV
AQGCDLIDKVIENL
>Mature_333_residues
TILSPELKKKLSTPLKIGNFEVKSRVLQSPLSGVTDLVFRRLVRRHAPESMMYTEMVNATGLHYVKELPQIMEVDNNERP
ISIQLFDCRPDFLAEAAEMAVKEGADTVDINMGCPVNKITKNGGGSSLLRQPELAGEIVSKVVKAVPIPVTVKTRIGWSN
QEINILEFAQRMEDAGAQMLTLHGRTRAQGYNGPARWEWITKVKEILSIPVIANGDIFSVDAAIRCLEETRADGVMCSRG
TLGYPFLVGEIDYFLKNGVKKVSPTAAEKLECAKEHLRALWEYKGDRGIRQARKHLTWYSKGFPGAGELRGQLAKVQTVA
QGCDLIDKVIENL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=319, Percent_Identity=31.6614420062696, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI239788483, Length=287, Percent_Identity=27.5261324041812, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI239788462, Length=293, Percent_Identity=27.3037542662116, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.8888888888889, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI8923374, Length=157, Percent_Identity=35.031847133758, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=34.3653250773994, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=37.2384937238494, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=25.7861635220126, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=310, Percent_Identity=29.6774193548387, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=251, Percent_Identity=28.2868525896414, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=255, Percent_Identity=30.5882352941176, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=33.1896551724138, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323437, Length=324, Percent_Identity=26.5432098765432, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=319, Percent_Identity=24.1379310344828, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=38.0530973451327, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=38.0530973451327, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=30.7359307359307, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585320, Length=292, Percent_Identity=29.4520547945205, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=136, Percent_Identity=33.0882352941176, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37107; Mature: 36975

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILSPELKKKLSTPLKIGNFEVKSRVLQSPLSGVTDLVFRRLVRRHAPESMMYTEMVNA
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TGLHYVKELPQIMEVDNNERPISIQLFDCRPDFLAEAAEMAVKEGADTVDINMGCPVNKI
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TKNGGGSSLLRQPELAGEIVSKVVKAVPIPVTVKTRIGWSNQEINILEFAQRMEDAGAQM
HCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEE
LTLHGRTRAQGYNGPARWEWITKVKEILSIPVIANGDIFSVDAAIRCLEETRADGVMCSR
EEECCCHHCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHCCCCEEECC
GTLGYPFLVGEIDYFLKNGVKKVSPTAAEKLECAKEHLRALWEYKGDRGIRQARKHLTWY
CCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SKGFPGAGELRGQLAKVQTVAQGCDLIDKVIENL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TILSPELKKKLSTPLKIGNFEVKSRVLQSPLSGVTDLVFRRLVRRHAPESMMYTEMVNA
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TGLHYVKELPQIMEVDNNERPISIQLFDCRPDFLAEAAEMAVKEGADTVDINMGCPVNKI
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TKNGGGSSLLRQPELAGEIVSKVVKAVPIPVTVKTRIGWSNQEINILEFAQRMEDAGAQM
HCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEE
LTLHGRTRAQGYNGPARWEWITKVKEILSIPVIANGDIFSVDAAIRCLEETRADGVMCSR
EEECCCHHCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHCCCCEEECC
GTLGYPFLVGEIDYFLKNGVKKVSPTAAEKLECAKEHLRALWEYKGDRGIRQARKHLTWY
CCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SKGFPGAGELRGQLAKVQTVAQGCDLIDKVIENL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]