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The map label for this gene is proS

Identifier: 113475053

GI number: 113475053

Start: 2011162

End: 2012967

Strand: Reverse

Name: proS

Synonym: Tery_1318

Alternate gene names: 113475053

Gene position: 2012967-2011162 (Counterclockwise)

Preceding gene: 113475055

Following gene: 113475052

Centisome position: 25.97

GC content: 39.53

Gene sequence:

>1806_bases
ATGCGACTATCTCAAATGTTATTGGTCACTTTACGGGATGACCCTGCTGAAGCAGAAATTCCTAGCCACAAACTCCTCAT
CCGTGCTGGATATATTCGTCGTATTGGTAATGGTATTTACGCATATCTCCCCTTAATGTTGCGAGTCATCAATAAAGTTT
CCACAATTGTTCGAGAAGAAATGAACGCTACTGGCGCCCAAGAATGTTTGCTTCCCCAACTCCAACCCGCCGAACTTTGG
CAAGAGTCTGGTCGCTGGGATACTTATACCCAGGCTGAGGGTATCATGTTTTCTTTAATTGACCGTCAAAATCGAGAATT
AGGTTTAGGGCCAACTCATGAAGAGGTAATAACTACTATTGCTAAAGATATTATTCGTTCCTATAGGCAACTACCCATAC
ATCTCTATCAACTTCAAACTAAATTCCGAGATGAAATTCGTCCTAGATTTGGGTTAATGCGAGGTCGAGAATTTATTATG
AAAGATGGCTATTCCTTTCATGCTGATGAGGAAAGTCTGAAAAAAACTTATCAGGATATGGATAAGGCTTATCGAAATAT
GTTACGTCGCAGTGGGTTACAGTTTCGGGCCGTTGATGCTGATTCTGGGGCCATAGGTGGTTCTGCTTCCCAAGAATTTA
TGGTTTTGGCCGATGCTGGCGAGGATGAAATTCTCTATACCCAAGATGGTAAGTATGCGGCAAATATGGAAAAGGCTATC
TCGTTGCCTGTAGATGTAGAAGCTTCACCTTTTGATACTTATGACAAATTGGAAACTCCTGGTACAGAAACTATTGAAAA
GATGTGTGAGTTTCTCCATTGTTCTGCAACTAATATAGTCAAAAATGTTTTGTACCAAGCCGTTTATGATAATGGGATTA
GTGTTTTAGTATTGGTGAGTATTCGGGGAGACCAAGATGTTAATGATGTGAAGTTGTTGAATGAGTTGACTAGGTTAGCT
GGAAATTATGAGGCCAAAACTGTTTTGGCTTTGAGTGTACCGGATGTGGAAGCTCAAAAGAAATGGGCAGCTAAGTCTTT
ACCTTTGGGTTATATTGCTCCAGATTTATCTGATGATTATATCACTTCTAGTAAACAGGTTAGTCCTAAGTTCCTACGAA
TGGTCGATAAAACAGCAGTATACTTGACAAATTTTGCAACTGGTAGTAATGAACTAGGTAATCATATTGTGGGGGCAAAT
TGGGGTCAAGAATTTGCTTTGCCAAAGTTGGTGGTAGATGTGCGAAAGGCTAAAAAAGGCGATCGCGCTGTACATGACCC
TAGTAAAACTCTAGAAACTGCTAGAGGCATTGAGGTGGGTCATATATTTCAGTTGGGGACAAAATATTCTGAAGCTATGG
GAGCAACCTATACAAATGAACAGGGCCAGGAAGTACCATTGTTAATGGGATGTTATGGTGTGGGAGTGTCTCGTTTAGCC
CAAGCGGCAGTAGAACAGTCTTATGACAAAGATGGTATAATTTGGCCTGTGGCTATTGCTCCCTATCATGTAGTTATTTG
TATTCCTAATATTAAAGATACTCAACAAATAGAAGTAGCAGGAAATCTTTATAAGGAATTAAATGAAGCAGGAATAGACA
CTATTTTAGATGACCGAGATGAACGTGCAGGTGTTAAATTTAAAGATGCAGATTTAATTGGTATTCCCTATCGAATAGTT
ACTGGGCGATCATTAAAATCTGGCAAGGTTGAACTTATAGAAAGATCTACTCACCAATGCCAGGAAATTGTTGTCGCGCA
AGTATTATCTACCTTAAAAGACTTAATTGAGAAAGCATTAAAATAA

Upstream 100 bases:

>100_bases
GTAATAAACGATAAAGTATTACCCTTTGCTTTTTGTTTAGCAATATACTAGATGTCTTAAACTAATCTGAGATAATCATT
ATTTTTACCAAGGAGCAACA

Downstream 100 bases:

>100_bases
TTTATTCACCCTTGAAAAATTCAAATATAATTTTTCCCTTATTAATTATTGCTGTGTTTTTACTAATAGGACTTACACCA
CTTAATCAGTCAAATTGAAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MRLSQMLLVTLRDDPAEAEIPSHKLLIRAGYIRRIGNGIYAYLPLMLRVINKVSTIVREEMNATGAQECLLPQLQPAELW
QESGRWDTYTQAEGIMFSLIDRQNRELGLGPTHEEVITTIAKDIIRSYRQLPIHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHADEESLKKTYQDMDKAYRNMLRRSGLQFRAVDADSGAIGGSASQEFMVLADAGEDEILYTQDGKYAANMEKAI
SLPVDVEASPFDTYDKLETPGTETIEKMCEFLHCSATNIVKNVLYQAVYDNGISVLVLVSIRGDQDVNDVKLLNELTRLA
GNYEAKTVLALSVPDVEAQKKWAAKSLPLGYIAPDLSDDYITSSKQVSPKFLRMVDKTAVYLTNFATGSNELGNHIVGAN
WGQEFALPKLVVDVRKAKKGDRAVHDPSKTLETARGIEVGHIFQLGTKYSEAMGATYTNEQGQEVPLLMGCYGVGVSRLA
QAAVEQSYDKDGIIWPVAIAPYHVVICIPNIKDTQQIEVAGNLYKELNEAGIDTILDDRDERAGVKFKDADLIGIPYRIV
TGRSLKSGKVELIERSTHQCQEIVVAQVLSTLKDLIEKALK

Sequences:

>Translated_601_residues
MRLSQMLLVTLRDDPAEAEIPSHKLLIRAGYIRRIGNGIYAYLPLMLRVINKVSTIVREEMNATGAQECLLPQLQPAELW
QESGRWDTYTQAEGIMFSLIDRQNRELGLGPTHEEVITTIAKDIIRSYRQLPIHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHADEESLKKTYQDMDKAYRNMLRRSGLQFRAVDADSGAIGGSASQEFMVLADAGEDEILYTQDGKYAANMEKAI
SLPVDVEASPFDTYDKLETPGTETIEKMCEFLHCSATNIVKNVLYQAVYDNGISVLVLVSIRGDQDVNDVKLLNELTRLA
GNYEAKTVLALSVPDVEAQKKWAAKSLPLGYIAPDLSDDYITSSKQVSPKFLRMVDKTAVYLTNFATGSNELGNHIVGAN
WGQEFALPKLVVDVRKAKKGDRAVHDPSKTLETARGIEVGHIFQLGTKYSEAMGATYTNEQGQEVPLLMGCYGVGVSRLA
QAAVEQSYDKDGIIWPVAIAPYHVVICIPNIKDTQQIEVAGNLYKELNEAGIDTILDDRDERAGVKFKDADLIGIPYRIV
TGRSLKSGKVELIERSTHQCQEIVVAQVLSTLKDLIEKALK
>Mature_601_residues
MRLSQMLLVTLRDDPAEAEIPSHKLLIRAGYIRRIGNGIYAYLPLMLRVINKVSTIVREEMNATGAQECLLPQLQPAELW
QESGRWDTYTQAEGIMFSLIDRQNRELGLGPTHEEVITTIAKDIIRSYRQLPIHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHADEESLKKTYQDMDKAYRNMLRRSGLQFRAVDADSGAIGGSASQEFMVLADAGEDEILYTQDGKYAANMEKAI
SLPVDVEASPFDTYDKLETPGTETIEKMCEFLHCSATNIVKNVLYQAVYDNGISVLVLVSIRGDQDVNDVKLLNELTRLA
GNYEAKTVLALSVPDVEAQKKWAAKSLPLGYIAPDLSDDYITSSKQVSPKFLRMVDKTAVYLTNFATGSNELGNHIVGAN
WGQEFALPKLVVDVRKAKKGDRAVHDPSKTLETARGIEVGHIFQLGTKYSEAMGATYTNEQGQEVPLLMGCYGVGVSRLA
QAAVEQSYDKDGIIWPVAIAPYHVVICIPNIKDTQQIEVAGNLYKELNEAGIDTILDDRDERAGVKFKDADLIGIPYRIV
TGRSLKSGKVELIERSTHQCQEIVVAQVLSTLKDLIEKALK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=231, Percent_Identity=42.4242424242424, Blast_Score=192, Evalue=6e-49,
Organism=Escherichia coli, GI1786392, Length=595, Percent_Identity=43.8655462184874, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI115532348, Length=254, Percent_Identity=33.8582677165354, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=223, Percent_Identity=37.6681614349776, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=217, Percent_Identity=37.7880184331797, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_TRIEI (Q116D3)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_721114.1
- ProteinModelPortal:   Q116D3
- SMR:   Q116D3
- STRING:   Q116D3
- GeneID:   4242469
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_1318
- NMPDR:   fig|203124.1.peg.4857
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q116D3
- ProtClustDB:   PRK09194
- BioCyc:   TERY203124:TERY_1318-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 67125; Mature: 67125

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQMLLVTLRDDPAEAEIPSHKLLIRAGYIRRIGNGIYAYLPLMLRVINKVSTIVREE
CCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNATGAQECLLPQLQPAELWQESGRWDTYTQAEGIMFSLIDRQNRELGLGPTHEEVITTI
HCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHCCHHHEECCCCCCCCCCCCCHHHHHHHH
AKDIIRSYRQLPIHLYQLQTKFRDEIRPRFGLMRGREFIMKDGYSFHADEESLKKTYQDM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHH
DKAYRNMLRRSGLQFRAVDADSGAIGGSASQEFMVLADAGEDEILYTQDGKYAANMEKAI
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHCHHHHH
SLPVDVEASPFDTYDKLETPGTETIEKMCEFLHCSATNIVKNVLYQAVYDNGISVLVLVS
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQDVNDVKLLNELTRLAGNYEAKTVLALSVPDVEAQKKWAAKSLPLGYIAPDLSDDY
ECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEECCCCCCHH
ITSSKQVSPKFLRMVDKTAVYLTNFATGSNELGNHIVGANWGQEFALPKLVVDVRKAKKG
CCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DRAVHDPSKTLETARGIEVGHIFQLGTKYSEAMGATYTNEQGQEVPLLMGCYGVGVSRLA
CCCCCCCHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHH
QAAVEQSYDKDGIIWPVAIAPYHVVICIPNIKDTQQIEVAGNLYKELNEAGIDTILDDRD
HHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCC
ERAGVKFKDADLIGIPYRIVTGRSLKSGKVELIERSTHQCQEIVVAQVLSTLKDLIEKAL
HHCCCEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MRLSQMLLVTLRDDPAEAEIPSHKLLIRAGYIRRIGNGIYAYLPLMLRVINKVSTIVREE
CCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNATGAQECLLPQLQPAELWQESGRWDTYTQAEGIMFSLIDRQNRELGLGPTHEEVITTI
HCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHCCHHHEECCCCCCCCCCCCCHHHHHHHH
AKDIIRSYRQLPIHLYQLQTKFRDEIRPRFGLMRGREFIMKDGYSFHADEESLKKTYQDM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHH
DKAYRNMLRRSGLQFRAVDADSGAIGGSASQEFMVLADAGEDEILYTQDGKYAANMEKAI
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHCHHHHH
SLPVDVEASPFDTYDKLETPGTETIEKMCEFLHCSATNIVKNVLYQAVYDNGISVLVLVS
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQDVNDVKLLNELTRLAGNYEAKTVLALSVPDVEAQKKWAAKSLPLGYIAPDLSDDY
ECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEECCCCCCHH
ITSSKQVSPKFLRMVDKTAVYLTNFATGSNELGNHIVGANWGQEFALPKLVVDVRKAKKG
CCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DRAVHDPSKTLETARGIEVGHIFQLGTKYSEAMGATYTNEQGQEVPLLMGCYGVGVSRLA
CCCCCCCHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHH
QAAVEQSYDKDGIIWPVAIAPYHVVICIPNIKDTQQIEVAGNLYKELNEAGIDTILDDRD
HHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCC
ERAGVKFKDADLIGIPYRIVTGRSLKSGKVELIERSTHQCQEIVVAQVLSTLKDLIEKAL
HHCCCEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA