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The map label for this gene is nudF [H]

Identifier: 113474898

GI number: 113474898

Start: 1768286

End: 1768873

Strand: Reverse

Name: nudF [H]

Synonym: Tery_1124

Alternate gene names: 113474898

Gene position: 1768873-1768286 (Counterclockwise)

Preceding gene: 113474900

Following gene: 113474897

Centisome position: 22.82

GC content: 31.8

Gene sequence:

>588_bases
ATGATATTATGGTTAAAGTTGAGTTTTTTATGTTTTATGGAAAATCAAGAATCCCCTGAAATTATTAAACAACGTTTGTT
ATATAAAGGTAGAAAATTTAACTTTGATGTTAACTATTTACGTCTACCTAATGGAGTTGAAGGTGACTGGGAATGTATCC
GTCATCCTGGAGGTGCTTTATGTATTCCTGTTACCTCAGATGGAAAATTAGTTTTAGTAAAACAATATAGATTTGCTATT
CAAGGCAGGATTTTAGAATTTCCCGCAGGTACAATAGAGGAAAATGAAAATCCTGCTGACACTGTTAAACGAGAAATAGA
AGAAGAAACAGGTTATCGAGCAAATAAATGGCAAAAATTAGGAGAGTTTCCCCTTGCTCCTGGATATTCAGATGAATTTA
TTTATGCTTTTTTGGCGGAAGATTTAGAAAAGTTAGAAAATCCTCCAGCACAAGATATTGATGAAGATATGGAAACAGTT
TTAATGACTCCTCAAGAATTAGAAAAAGCTATCTTAGAAGGAGAATTTATAGATGCTAAATCAATCTCTAGTTTATTTTT
AGCAAAACCATTTTTATCACGCATATAA

Upstream 100 bases:

>100_bases
AATTTTATTGATTTCTGGGTAAGTAAAAAATAAATAGCTTAAACTAATTAGAATATTTATAACTATCAAGTTTAACCATT
TAACCTATATTTATACAAAG

Downstream 100 bases:

>100_bases
AAGCTTTACTAACAATGGTTGAAACGATTAAAGTAACCCTTAAAAATTGGGACCAATAGTAAAAATGATCATAGAGTTGT
TGGGTATGGGAAAATTGAAA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MILWLKLSFLCFMENQESPEIIKQRLLYKGRKFNFDVNYLRLPNGVEGDWECIRHPGGALCIPVTSDGKLVLVKQYRFAI
QGRILEFPAGTIEENENPADTVKREIEEETGYRANKWQKLGEFPLAPGYSDEFIYAFLAEDLEKLENPPAQDIDEDMETV
LMTPQELEKAILEGEFIDAKSISSLFLAKPFLSRI

Sequences:

>Translated_195_residues
MILWLKLSFLCFMENQESPEIIKQRLLYKGRKFNFDVNYLRLPNGVEGDWECIRHPGGALCIPVTSDGKLVLVKQYRFAI
QGRILEFPAGTIEENENPADTVKREIEEETGYRANKWQKLGEFPLAPGYSDEFIYAFLAEDLEKLENPPAQDIDEDMETV
LMTPQELEKAILEGEFIDAKSISSLFLAKPFLSRI
>Mature_195_residues
MILWLKLSFLCFMENQESPEIIKQRLLYKGRKFNFDVNYLRLPNGVEGDWECIRHPGGALCIPVTSDGKLVLVKQYRFAI
QGRILEFPAGTIEENENPADTVKREIEEETGYRANKWQKLGEFPLAPGYSDEFIYAFLAEDLEKLENPPAQDIDEDMETV
LMTPQELEKAILEGEFIDAKSISSLFLAKPFLSRI

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=180, Percent_Identity=26.1111111111111, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22425; Mature: 22425

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILWLKLSFLCFMENQESPEIIKQRLLYKGRKFNFDVNYLRLPNGVEGDWECIRHPGGAL
CEEEEEEEEHHEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEE
CIPVTSDGKLVLVKQYRFAIQGRILEFPAGTIEENENPADTVKREIEEETGYRANKWQKL
EEEECCCCCEEEEEEHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
GEFPLAPGYSDEFIYAFLAEDLEKLENPPAQDIDEDMETVLMTPQELEKAILEGEFIDAK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCHH
SISSLFLAKPFLSRI
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MILWLKLSFLCFMENQESPEIIKQRLLYKGRKFNFDVNYLRLPNGVEGDWECIRHPGGAL
CEEEEEEEEHHEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEE
CIPVTSDGKLVLVKQYRFAIQGRILEFPAGTIEENENPADTVKREIEEETGYRANKWQKL
EEEECCCCCEEEEEEHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
GEFPLAPGYSDEFIYAFLAEDLEKLENPPAQDIDEDMETVLMTPQELEKAILEGEFIDAK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCHH
SISSLFLAKPFLSRI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]