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The map label for this gene is cocE [H]

Identifier: 113474308

GI number: 113474308

Start: 688950

End: 690572

Strand: Reverse

Name: cocE [H]

Synonym: Tery_0437

Alternate gene names: 113474308

Gene position: 690572-688950 (Counterclockwise)

Preceding gene: 113474311

Following gene: 113474306

Centisome position: 8.91

GC content: 35.8

Gene sequence:

>1623_bases
ATGAAAATGAAAATCTTACCCAAAGAAACTACTTCTATATTTACTAGAGATGGTATACGTTTAGATGCTGATATTTATCG
CCCTGATGCAGCAGGTGAATTTCCAGTAATATTAATGCGACAACCTTATGGCAGAGCGATCGCCTCAACGGTAGTCTATG
CTCACCCAACATGGTATGCTGCTCATGGTTATATTGTGATTATTCAAGATGTGCGGGGTCGGGGTACATCAGAGGGTGAA
TTTGAATTATTTGCCCATGAAATTGAAGATGGAATTGATACTATTAATTGGGCTGCAAATTTACCAGGTAGCACTGGGGA
AATAGGAATGTATGGATTTTCTTATCAAGGCATGACTCAACTATATGCTGCTAGTGCTAAATCTCCTGTTATAAAAACAA
TTTGTCCGGCAATGATTGGTTATAATTTATATACAGACTGGGCTTATGAAGGAGGGGCTTTTTGTCTACAAACAAATCTA
GGTTGGGCAGTTCAACTAGCAACAGAAACTGCTCATTTACGAGGAGATGAACAAGCCTATCAAGAGCTTTATCAAGCTTC
GAAAAATCTGCCTTTATCTACTACAATTCCTGCTTTACCTGAAGTGATTAATGAGTATGGAAAAGATTCTTTTTATCATC
AGTGGATAGAAAATTATCAACCTGGTGAATATTGGCAAAACTTATCACCAAATATGAAAAATGTAGATCTACCAATTCTA
CATATCGGCGGTTGGTTTGATACTTATTTACGGGGAACAATTCATTTATATAAAGAAATGGCTGGTCGGAGTAAATTCTT
ACAAAAATTAATTGTAGGTCCTTGGTCTCATATTCCTTGGGGCAGAAAAGTAGGAGCTGTAAATTATGGTTTAGGAGCTT
CTAGTTTTATAGATGATATACAAATATTATGGTTTAATCAGTTTCTCAAAGGAATAAATACAGGTATATTTGAGCGATCG
CCTGTTTCTTTATTTGAGATGGGAAGTAATGAATGGCGTAATTTAAATTCCTGGCCGAGTAACAATTACAAGTTCTATTA
TTTGGCTAGTACAGGACTTGCTAATGTTAGAGAAGATTCGGGGAGTCTCACGCAAAGCTGTCCTAATATTTCTGCTGATG
ATAAATTCGTACATGATCCTTGGCGACCTGTTCCAGCATTAGGGGGTCATGCAACGTTTCCTGCTGGTTCTTTTGAACGT
TCAGAATTAGATTGTCGTACAGATATTTTGACTTATACTTCTGAGTTTTTCAAGGAAGATTTACACATAGCAGGAGACAT
TTCTGTAGATTTACATTGTACAGCAGATACAAGTAGTTTTGACCTTTCTGCAGTGTTATCTGAAGTGACAAAAAATGGGA
ATGTTTACAATTTTACTCAAGGTTATATCAAGGTTATTGATAATAATTTACCTGTGACAATTTGTTTGCAACCAACTTGT
ATGAAAATTCAGAAAGGTAATGCTATAAGATTGAGCTTAAGCGCAGCTTGTTTTCCTGCTTATATAATTAATCCAGGAAT
AGATAAATCACCAGGGGAAGTAAGATTAATTGACTCCAAAATTATTACGATAACTGTTAGTTCAGGAAAAAATTATCCTT
CTGTAATTTCTCTGTCTGTCTAA

Upstream 100 bases:

>100_bases
GGATTGCTATATATTGTTTTATAATTGCTTTTATCCTTGCTACTAAGTCCCTATTCCCTACTCCCTAAAAATACTTTTTT
AGCAAACCCTATTTACAAAT

Downstream 100 bases:

>100_bases
ATTGAAATAGTTGAAGATTAGGAATAATAAATAAGGGGAAAAATAACGTAAATTTGTTTACATATTCCTAGCCATAAACT
CTCAGGGATCCTCACTTTAT

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKMKILPKETTSIFTRDGIRLDADIYRPDAAGEFPVILMRQPYGRAIASTVVYAHPTWYAAHGYIVIIQDVRGRGTSEGE
FELFAHEIEDGIDTINWAANLPGSTGEIGMYGFSYQGMTQLYAASAKSPVIKTICPAMIGYNLYTDWAYEGGAFCLQTNL
GWAVQLATETAHLRGDEQAYQELYQASKNLPLSTTIPALPEVINEYGKDSFYHQWIENYQPGEYWQNLSPNMKNVDLPIL
HIGGWFDTYLRGTIHLYKEMAGRSKFLQKLIVGPWSHIPWGRKVGAVNYGLGASSFIDDIQILWFNQFLKGINTGIFERS
PVSLFEMGSNEWRNLNSWPSNNYKFYYLASTGLANVREDSGSLTQSCPNISADDKFVHDPWRPVPALGGHATFPAGSFER
SELDCRTDILTYTSEFFKEDLHIAGDISVDLHCTADTSSFDLSAVLSEVTKNGNVYNFTQGYIKVIDNNLPVTICLQPTC
MKIQKGNAIRLSLSAACFPAYIINPGIDKSPGEVRLIDSKIITITVSSGKNYPSVISLSV

Sequences:

>Translated_540_residues
MKMKILPKETTSIFTRDGIRLDADIYRPDAAGEFPVILMRQPYGRAIASTVVYAHPTWYAAHGYIVIIQDVRGRGTSEGE
FELFAHEIEDGIDTINWAANLPGSTGEIGMYGFSYQGMTQLYAASAKSPVIKTICPAMIGYNLYTDWAYEGGAFCLQTNL
GWAVQLATETAHLRGDEQAYQELYQASKNLPLSTTIPALPEVINEYGKDSFYHQWIENYQPGEYWQNLSPNMKNVDLPIL
HIGGWFDTYLRGTIHLYKEMAGRSKFLQKLIVGPWSHIPWGRKVGAVNYGLGASSFIDDIQILWFNQFLKGINTGIFERS
PVSLFEMGSNEWRNLNSWPSNNYKFYYLASTGLANVREDSGSLTQSCPNISADDKFVHDPWRPVPALGGHATFPAGSFER
SELDCRTDILTYTSEFFKEDLHIAGDISVDLHCTADTSSFDLSAVLSEVTKNGNVYNFTQGYIKVIDNNLPVTICLQPTC
MKIQKGNAIRLSLSAACFPAYIINPGIDKSPGEVRLIDSKIITITVSSGKNYPSVISLSV
>Mature_540_residues
MKMKILPKETTSIFTRDGIRLDADIYRPDAAGEFPVILMRQPYGRAIASTVVYAHPTWYAAHGYIVIIQDVRGRGTSEGE
FELFAHEIEDGIDTINWAANLPGSTGEIGMYGFSYQGMTQLYAASAKSPVIKTICPAMIGYNLYTDWAYEGGAFCLQTNL
GWAVQLATETAHLRGDEQAYQELYQASKNLPLSTTIPALPEVINEYGKDSFYHQWIENYQPGEYWQNLSPNMKNVDLPIL
HIGGWFDTYLRGTIHLYKEMAGRSKFLQKLIVGPWSHIPWGRKVGAVNYGLGASSFIDDIQILWFNQFLKGINTGIFERS
PVSLFEMGSNEWRNLNSWPSNNYKFYYLASTGLANVREDSGSLTQSCPNISADDKFVHDPWRPVPALGGHATFPAGSFER
SELDCRTDILTYTSEFFKEDLHIAGDISVDLHCTADTSSFDLSAVLSEVTKNGNVYNFTQGYIKVIDNNLPVTICLQPTC
MKIQKGNAIRLSLSAACFPAYIINPGIDKSPGEVRLIDSKIITITVSSGKNYPSVISLSV

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 60171; Mature: 60171

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKILPKETTSIFTRDGIRLDADIYRPDAAGEFPVILMRQPYGRAIASTVVYAHPTWYA
CCEEECCCCHHHEEECCCEEEECEEECCCCCCCCCEEEEECCCCHHHHHEEEEECCCEEE
AHGYIVIIQDVRGRGTSEGEFELFAHEIEDGIDTINWAANLPGSTGEIGMYGFSYQGMTQ
CCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCHHH
LYAASAKSPVIKTICPAMIGYNLYTDWAYEGGAFCLQTNLGWAVQLATETAHLRGDEQAY
HHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCEEEEEEEHHHHCCCCHHHH
QELYQASKNLPLSTTIPALPEVINEYGKDSFYHQWIENYQPGEYWQNLSPNMKNVDLPIL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEE
HIGGWFDTYLRGTIHLYKEMAGRSKFLQKLIVGPWSHIPWGRKVGAVNYGLGASSFIDDI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEECCCCCHHHHHHH
QILWFNQFLKGINTGIFERSPVSLFEMGSNEWRNLNSWPSNNYKFYYLASTGLANVREDS
HHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
GSLTQSCPNISADDKFVHDPWRPVPALGGHATFPAGSFERSELDCRTDILTYTSEFFKED
CCHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LHIAGDISVDLHCTADTSSFDLSAVLSEVTKNGNVYNFTQGYIKVIDNNLPVTICLQPTC
CCEEECEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCCEEEEECCCE
MKIQKGNAIRLSLSAACFPAYIINPGIDKSPGEVRLIDSKIITITVSSGKNYPSVISLSV
EEEECCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECEEEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure
MKMKILPKETTSIFTRDGIRLDADIYRPDAAGEFPVILMRQPYGRAIASTVVYAHPTWYA
CCEEECCCCHHHEEECCCEEEECEEECCCCCCCCCEEEEECCCCHHHHHEEEEECCCEEE
AHGYIVIIQDVRGRGTSEGEFELFAHEIEDGIDTINWAANLPGSTGEIGMYGFSYQGMTQ
CCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCHHH
LYAASAKSPVIKTICPAMIGYNLYTDWAYEGGAFCLQTNLGWAVQLATETAHLRGDEQAY
HHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCEEEEEEEHHHHCCCCHHHH
QELYQASKNLPLSTTIPALPEVINEYGKDSFYHQWIENYQPGEYWQNLSPNMKNVDLPIL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEE
HIGGWFDTYLRGTIHLYKEMAGRSKFLQKLIVGPWSHIPWGRKVGAVNYGLGASSFIDDI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEECCCCCHHHHHHH
QILWFNQFLKGINTGIFERSPVSLFEMGSNEWRNLNSWPSNNYKFYYLASTGLANVREDS
HHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
GSLTQSCPNISADDKFVHDPWRPVPALGGHATFPAGSFERSELDCRTDILTYTSEFFKED
CCHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LHIAGDISVDLHCTADTSSFDLSAVLSEVTKNGNVYNFTQGYIKVIDNNLPVTICLQPTC
CCEEECEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCCEEEEECCCE
MKIQKGNAIRLSLSAACFPAYIINPGIDKSPGEVRLIDSKIITITVSSGKNYPSVISLSV
EEEECCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECEEEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]